Physiological and biochemical responses of potato (Solanum tuberosum) to silver nanoparticles and silver nitrate treatments under in vitro conditions

2015 ◽  
Vol 20 (4) ◽  
pp. 353-359 ◽  
Author(s):  
Mozafar Bagherzadeh Homaee ◽  
Ali Akbar Ehsanpour
Keyword(s):  
Silver Nanoparticles ◽  
Solanum Tuberosum ◽  
Silver Nitrate ◽  
Biochemical Responses ◽  
Physiological And Biochemical

scholarly journals The Design, Characterization and Antibacterial Activity of Heat and Silver Crosslinked Poly(Vinyl Alcohol) Hydrogel Forming Dressings Containing Silver Nanoparticles

Nanomaterials ◽  
2021 ◽  
Vol 11 (1) ◽  
pp. 96
Author(s):  
John Jackson ◽  
Helen Burt ◽  
Dirk Lange ◽  
In Whang ◽  
Robin Evans ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
Antibacterial Activity ◽  
Silver Nitrate ◽  
Vinyl Alcohol ◽  
Unmet Need ◽  
Chemical Characterization ◽  
Wound Dressings ◽  
Poly Vinyl Alcohol ◽  
Burn Wound

The prompt treatment of burn wounds is essential but can be challenging in remote parts of Africa, where burns from open fires are a constant hazard for children and suitable medical care may be far away. Consequently, there is an unmet need for an economical burn wound dressing with a sustained antimicrobial activity that might be manufactured locally at low cost. This study describes and characterizes the novel preparation of a silver nitrate-loaded/poly(vinyl alcohol) (PVA) film. Using controlled heating cycles, films may be crosslinked with in situ silver nanoparticle production using only a low heat oven and little technical expertise. Our research demonstrated that heat-curing of PVA/silver nitrate films converted the silver to nanoparticles. These films swelled in water to form a robust, wound-compatible hydrogel which exhibited controlled release of the antibacterial silver nanoparticles. An optimal formulation was obtained using 5% (w/w) silver nitrate in PVA membrane films that had been heated at 140 °C for 90 min. Physical and chemical characterization of such films was complemented by in vitro studies that confirmed the effective antibacterial activity of the released silver nanoparticles against both gram positive and negative bacteria. Overall, these findings provide economical and simple methods to manufacture stable, hydrogel forming wound dressings that release antibiotic silver over prolonged periods suitable for emergency use in remote locations.

Physiological and Biochemical Responses of Merwilla plumbea Cultured In Vitro with Different Cytokinins After 1 Year of Growth Under Ex Vitro Conditions

2016 ◽  
Vol 36 (1) ◽  
pp. 83-95
Author(s):  
Mack Moyo ◽  
Stephen O. Amoo ◽  
Adeyemi O. Aremu ◽  
Lucie Plíhalová ◽  
Jiří Gruz ◽  
...  
Keyword(s):  
Ex Vitro ◽  
Biochemical Responses ◽  
Physiological And Biochemical

Viburnum nervosum Leaf Extract Mediated Green Synthesis of Silver Nanoparticles: A Viable Approach to Increase the Efficacy of Anticancer Drug

2020 ◽  
Vol 20 ◽  
Author(s):  
Irfana Zahoor ◽  
Farhat Jan ◽  
Ujjawal Sharma ◽  
Kiran K. Sahu ◽  
Amita Sharma ◽  
...  
Keyword(s):  
Drug Delivery ◽  
Silver Nanoparticles ◽  
Green Synthesis ◽  
Silver Nitrate ◽  
Leaf Extract ◽  
Analytical Techniques ◽  
Therapeutic Interventions ◽  
A431 Cells ◽  
Anti Cancer

Background:: There is an urgent need to devise improved alternatives for the efficient delivery of drugs to develop improved therapeutic interventions against cancers. Nanotechnology based drug delivery vehicles are in-use with obvious issues of toxicity and bio-distribution. Therefore, green synthetic routes are being deployed to replace the conventional nanoparticle formulations for effective drug delivery aiming at developing interventional strategies against cancer. Objective:: A simple, viable and fast approach was used for the green synthesis of silver nanoparticles (AgNPs) using aqueous leaf-extract of Viburnum nervosum (VN) and to explore the anti-cancer potential of the crude extract of VN. Methods:: Silver NPs were synthesized by reacting silver nitrate (AgNO3) with leaf extract of VN. Various analytical techniques were used to characterize the AgNPs. Finally, the anti-cancer potential of VN was observed when delivered through AgNPs. Results:: The surface plasmon spectra for AgNPs exhibited absorbance peak at 445 nm, and Fourier-Transform Infrared Spectroscopy investigation revealed presence of biomolecules acting as an effective reducing and capping agent for converting silver nitrate to AgNPs. Further, our results suggest the spherical size of synthesized AgNPs ranging from 12-17 nm. Moreover, in vitro studies conducted for VN extract with breast (MCF-7) and epidermal carcinoma (A431) cells showed biocompatibility. Conclusion:: Doxorubicin loaded AgNPs documented an increased bioavailability of drug compared to the free drug, suggesting the use of AgNPs as “novel drug delivery vectors”.

Physiological and biochemical responses of in vitro Fusarium oxysporum f.sp. niveum to benzoic acid

2009 ◽  
Vol 54 (2) ◽  
pp. 115-122 ◽  
Author(s):  
H. -S. Wu ◽  
Y. Wang ◽  
C. -Y. Zhang ◽  
M. Gu ◽  
Y. -X. Liu ◽  
...  
Keyword(s):  
Benzoic Acid ◽  
Fusarium Oxysporum ◽  
Biochemical Responses ◽  
Physiological And Biochemical

scholarly journals Effect of water deficit on some physiological and biochemical responses of the yellow diploid potato (Solanum tuberosum L. Group Phureja)

2020 ◽  
Vol 38 (1) ◽  
pp. 36-44
Author(s):  
Wilmar Ariza ◽  
Luis Ernesto Rodríguez ◽  
Darwin Moreno-Echeverry ◽  
Carlos Arturo Guerrero ◽  
Liz Patricia Moreno
Keyword(s):  
Solanum Tuberosum ◽  
Stomatal Conductance ◽  
Water Deficit ◽  
Solanum Tuberosum L ◽  
Tuber Initiation ◽  
Diploid Potato ◽  
Biochemical Responses ◽  
Shoot Ratio ◽  
Root Shoot Ratio ◽  
Physiological And Biochemical

Water availability is one of the main limitations of potato yields due to the high sensitivity of this crop to water deficit. The objective of this study was to determine the effect of water deficit on some physiological and biochemical responses in yellow diploid potato plants (Solanum tuberosum L. Group Phureja) of the cultivars Criolla Colombia, Criolla Dorada and Criolla Ocarina. Plants at tuber initiation were subjected to two treatments: continuous irrigation and water deficit imposed by withholding water at tuber initiation for 17 d. The results showed that plants under water deficit increased chlorophyll concentration, malondialdehyde and proline content. However, these plants showed a decrease in stomatal conductance, leafarea, total dry mass and exhibited a higher root/shoot ratio in all potato cultivars. In addition, all the cultivars also showed a decrease in yield, which was associated with sensitivity to water stress. Although the high content of proline and high root/shoot ratio may be associated with tolerance to water deficit, this association was not observed in these cultivars, probably due to the high reduction of stomatal conductance, which limited the production of photoassimilates, plant growth, and,therefore, the yield.

scholarly journals Synthesis of Chitosan Stabilized Silver Nanoparticles and Evaluation of the in vitro Antibacterial Activity Against Xanthomonas oryzae pv. oryzae Causing Blight Disease of Rice

2021 ◽  
Author(s):  
Le Thi Hien ◽  
Nguyen Thi Phuong Hue ◽  
Le Trong Duc ◽  
Vu Thi Huyen ◽  
Le Thi Van ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
Antibacterial Activity ◽  
Silver Nitrate ◽  
Bacterial Blight ◽  
Xanthomonas Oryzae ◽  
Diffusion Method ◽  
Colloidal Systems ◽  
Potential Measurement ◽  
In Vitro Antibacterial Activity

Bacterial blight is one of the most devastating rice diseases that cause huge economic loss worldwide. The cause of rice blight is Gram negative bacteria Xanthomonas oryzae pv. oryzae (X. oryzae pv. Oryzae). Since both silver nanoparticles and chitosan have antibacterial, antifungal and growth-stimulating effect, this work has focused on synthesizing chitosan stabilized silver nanoparticles (AgCSs) with small sizes and in vitro evaluating antibacterial activity against X. oryzae pv. oryzae bacteria. AgCSs were chemically synthesized by reducing silver nitrate by borohydride sodium in the presence of chitosan with optimization of the concentration of the reactants. AgCSs were characterized by UV/vis absorption spectra, field emission scanning electronic microscopy (FESEM), ImageJ software, zeta potential measurement, Fourier-transform infrared spectroscopy (FTIR) and X-ray diffraction. AgCSs have spherical configuration and narrow size distributions with different average sizes from 15 nm to 25 nm depending on the initial concentration of silver nitrate. All AgCSs colloidal systems were stable and exhibited no tendency for coagulation more than 5 months. It was the first time that chitosan-stabilized silver nanoparticles were assessed the in vitro antibacterial activity against bacterial blight VXO_281 strain. The disc diffusion method demonstrated that the smallest size silver nanoparticles (AgCS1) showed high antibacterial effect against the X. oryzae pv. oryzae VXO_281 strain with a concentration of more than 5 µg/mL and the inhibition zone was dose-dependent. The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of AgCS1 against X. oryzae pv. oryzae VXO_281 were 2.5 µg/mL and 20 µg/mL, respectively.

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