In this paper are presented the results of some research made to obtain cellular lines tolerating salinity from embryogen callus and non embryogen red clover, from the di- and tetraploid genotypes to verify if the potential mutants, shown by us, may manifest some salinity tolerance. Our studies aimed both untreated biological material and chemical mutagens (DES and DMS).
It was observed that salty environment influences in a negative way the development of callus by decreasing growth and by necrosis appearance on areas more or less extended.
Under the aspect of the genotype reaction towards the salinity, the tetraploid cultivar has proved more tolerant, compared to the diploid one.
At a concentration of 0,5% NaCl the percentage of survival of the red clover callus is of 53,2% at Napoca-tetra cultivar, 23,4% at Apollo-tetra and 23,4% at Roxana diplod, respectively 21,0% at Select. At the concentration of 1,0% NaCl the callus growing is strongly inhibited, up to 19,3% at Napoca-tetra, 17,4% at Apollo-tetra and 4,3 and 3,2% at Roxana (2n), respectively Select (2n).
Following the genotypes behavior of red clover, which are potential mutants, resulted after the treatment with DMS and DES, pointed out positive reaction of the obtained callus at these plants, on medium culture with 0,5% NaCl.
The transfer of callus on culture medium with NaCl 1,0% points out also a growing of the callus surface, but in a low percent, especially at Roxana cultivar (2n).
Rapid in vitro selection of salt-tolerant genotypes of the potentially medicinal plant Centaurium maritimum (L.) fritsch