Bacillus methylotrophicus M4-96 Stimulates the Growth of Strawberry (Fragaria × ananassa ‘Aromas’) Plants In Vitro and Slows Botrytis cinerea Infection by Two Different Methods of Interaction

2018 ◽  
Vol 38 (3) ◽  
pp. 765-777 ◽  
Author(s):  
Alondra Vicente-Hernández ◽  
Rafael Salgado-Garciglia ◽  
Eduardo Valencia-Cantero ◽  
Arturo Ramírez-Ordorica ◽  
Alejandra Hernández-García ◽  
...  
Keyword(s):  
Botrytis Cinerea ◽  
Fragaria Ananassa ◽  
Bacillus Methylotrophicus

Endophytic bacteria Arthrobacter agilis UMCV2 and Bacillus methylotrophicus M4-96 stimulate achene germination, in vitro growth, and greenhouse yield of strawberry (Fragaria × ananassa)

2020 ◽  
Vol 261 ◽  
pp. 109005
Author(s):  
Christian Hernández-Soberano ◽  
León Francisco Ruíz-Herrera ◽  
Eduardo Valencia-Cantero
Keyword(s):  
Endophytic Bacteria ◽  
In Vitro Growth ◽  
Fragaria Ananassa ◽  
Bacillus Methylotrophicus ◽  
Arthrobacter Agilis

scholarly journals CONTROLE DE BOTRYTIS CINEREA NA PÓS-COLHEITA DO MORANGUEIRO (FRAGARIA SP.) COM DIFERENTES ÓLEOS ESSENCIAIS

2020 ◽  
Vol 1 (13) ◽  
Author(s):  
Monalisa Cristina de Cól ◽  
Elisiane Fachin Tozatti ◽  
Talita Durante Bosetti ◽  
Ian Carlos Galon ◽  
Ronaldo Paolo Paludo ◽  
...  
Keyword(s):  
Botrytis Cinerea ◽  
Rosmarinus Officinalis ◽  
Mentha Piperita ◽  
Fragaria Ananassa ◽  
Cinnamomum Cassia ◽  
Cedrus Atlantica

O morango (Fragaria × ananassa) é uma cultura cultivada em todo o mundo, alcançando cerca de 372.000 hectares (ha), sendo no Brasil utilizado 390ha para cultivo com rendimento de 8.926,8 kg/ha. Dentre as doenças fúngicas o mofo cinzento, ocasionado pelo patógeno Botrytis cinerea, é considerado de maior importância na pós colheita do morango. Essas recorrentes deteriorações pós-colheita influenciam na comercialização e preço do produto. Para o controle do B. cinerea muitas vezes são necessárias repetidas pulverizações de fungicidas. Assim, como alternativa, o controle utilizando óleos essenciais (OEs) pode fornecer benefícios ao substituir ou complementar os fungicidas. Nesse contexto, o objetivo desse trabalho foi avaliar a eficácia dos OEs de cedro, canela, menta e alecrim contra B. cinerea. O experimento foi realizado in vitro e conduzido em delineamento inteiramente casualizado, em esquema fatorial 4 x 4 (óleo x concentração), com três repetições, avaliado em triplicata. Foram utilizados OEs de Cedrus atlantica (Cedro Atlas), Cinnamomum cassia (Canela), Mentha piperita (Menta Piperita), e Rosmarinus officinalis (Alecrim). Para os testesde suscetibilidade de B. cinerea aos OEs foi feita a inoculação do fungo em ágar BDA contendo cada óleo nas concentrações de 0,1, 0,2, 0,4 e 0,8%, seguido da incubação em BOD à 24ºC. As medições do diâmetro das colônias foram realizadas a cada 48 horas durante 10 dias. Os valores obtidos foram utilizados para o cálculo da porcentagem de inibição do crescimento micelial (PIC). Os dados foram submetidos à análise de variância (ANOVA) e as médias relativas aos diferentes tipos de OEs foram comparadas pelo teste Scott-Knott (p<0,05) e as médias relativas as diferentes concentrações dos OEs foram submetidas a análise de regressão utilizando o software R. A análise de variância demonstrou que houve efeito significativo (p< 0,05) da interação óleo x concentração sobre a variável PIC. Considerando a média geral, os valores de PIC foram de 68% para cedro, 74% para alecrim, 93% para canela e 94% para menta. Para os OEs cedro, canela e menta, o aumento da concentração não resultou em diferença significativa na porcentagem de inibição sobre o crescimento do fungo, sendo todas as concentrações consideradas fungitóxicas. Já o óleo essencial (OE) de alecrim, à medida que se aumentou a concentração, houve aumento na porcentagem de inibição. Para a concentração 0,1% os OEs de canela e menta apresentaram maior PIC que alecrim e cedro. Na concentração 0,4% de canela, menta e alecrim obteve-se PIC superior ao cedro, enquanto que nas concentrações 0,2 e 0,8% não houve diferença significativa de PIC entre os OEs. O estudo demonstrou que todos os OEs testados possuem atividade antifúngica contra B. cinerea, contudo, os OEs de menta e canela, nas condições propostas neste estudo, demonstraram ser a alternativa mais promissora, para estudos visando aplicações in vivo.

Influence of salicylic acid on organogenesis of in vitro plants Fragaria ananassa Duch.

2016 ◽  
Vol 48 (1) ◽  
pp. 26-33
Author(s):  
O.V. Subin ◽  
◽  
M.D. Melnychuk ◽  
A.F. Likhanov ◽  
O.L. Klyachenko ◽  
...  
Keyword(s):  
Salicylic Acid ◽  
Fragaria Ananassa ◽  
In Vitro Plants

An Attempt at Biological Control of Blossom Blight of Rose Caused by Botrytis cinerea Using some Local Trichoderma spp. Strains

2020 ◽  
Vol 55 (1) ◽  
pp. 27-34
Author(s):  
G. Zadehdabagh ◽  
K. Karimi ◽  
M. Rezabaigi ◽  
F. Ajamgard
Keyword(s):  
Botrytis Cinerea ◽  
Mycelial Growth ◽  
Limiting Factor ◽  
Dual Culture ◽  
Trichoderma Spp ◽  
Khuzestan Province ◽  
Inhibitory Potential ◽  
Cut Rose

The northern of Khuzestan province in Iran is mainly considered as one of the major areas of miniature rose production. Blossom blight caused by Botrytis cinerea has recently become a serious limiting factor in rose production in pre and post-harvest. In current study, an attempt was made to evaluate the inhibitory potential of some local Trichoderma spp. strains against B. cinerea under in vitro and in vivo conditions. The in vitro results showed that all Trichoderma spp. strains were significantly able to reduce the mycelial growth of the pathogen in dual culture, volatile and non-volatile compounds tests compared with control, with superiority of T. atroviride Tsafi than others. Under in vivo condition, the selected strain of T. atroviride Tsafi had much better performance than T. harzianum IRAN 523C in reduction of disease severity compared with the untreated control. Overall, the findings of this study showed that the application of Trichoderma-based biocontrol agents such as T. atroviride Tsafi can be effective to protect cut rose flowers against blossom blight.

scholarly journals Synergistic Effect of Combined Application of a New Fungicide Fluopimomide with a Biocontrol Agent Bacillus methylotrophicus TA-1 for Management of Gray Mold in Tomato

Plant Disease ◽  
2019 ◽  
Vol 103 (8) ◽  
pp. 1991-1997 ◽  
Author(s):  
Xiaoxue Ji ◽  
Jingjing Li ◽  
Zhen Meng ◽  
Shouan Zhang ◽  
Bei Dong ◽  
...  
Keyword(s):  
Field Experiments ◽  
Biocontrol Agent ◽  
Severe Disease ◽  
Combined Treatment ◽  
Field Trials ◽  
Antimicrobial Activities ◽  
Gray Mold ◽  
Control Efficacy ◽  
Bacillus Methylotrophicus

Gray mold caused by Botrytis cinerea can be a severe disease of tomato infecting leaves and fruits of tomato plants. Chemical control is currently the most effective and reliable method; however, application of fungicides has many drawbacks. The combination of biological control agents with newly developed fungicides may be a practicable method to control B. cinerea. Fluopimomide is a newly developed fungicide with a novel mode of action. Bacillus methylotrophicus TA-1, isolated from rhizosphere soil of tomato, is a bacterial strain with a broad spectrum of antimicrobial activities. Little information is currently available about the effect of fluopimomide and its integrated effect on B. cinerea. Therefore, laboratory, pot, and field experiments were carried out to determine the effects of fluopimomide alone and in combination with B. methylotrophicus TA-1 against gray mold on tomato. The in vitro growth of B. methylotrophicus TA-1 was unaffected by 100 mg liter−1 fluopimomide. Inhibition of B. cinerea mycelial growth was significantly increased under combined treatment of fluopimomide and B. methylotrophicus TA-1. In greenhouse experiments, efficacy against gray mold was significantly greater by an integration of fluopimomide and B. methylotrophicus TA-1 than by either alone; control efficacy of fluopimomide at 50 and 100 g ha−1 in combination with B. methylotrophicus TA-1 at 108 colony-forming units (cfu) ml−1 reached 70.16 and 69.32%, respectively, compared with the untreated control. In both field trials during 2017 and 2018, control efficacy was significantly higher for the combination of fluopimomide at 50 and 100 g ha−1 in combination with B. methylotrophicus TA-1 than for either treatment alone. The results from this study indicated that integration of the new fungicide fluopimomide with the biocontrol agent B. methylotrophicus TA-1 synergistically increased control efficacy of the fungicide against gray mold of tomato.

scholarly journals Characterization of Postharvest Fungicide-Resistant Botrytis cinerea Isolates From Commercially Stored Apple Fruit

2017 ◽  
Vol 107 (3) ◽  
pp. 362-368 ◽  
Author(s):  
Wayne M. Jurick ◽  
Otilia Macarisin ◽  
Verneta L. Gaskins ◽  
Eunhee Park ◽  
Jiujiang Yu ◽  
...  
Keyword(s):  
Botrytis Cinerea ◽  
Gray Mold ◽  
Apple Fruit ◽  
Sublethal Dose ◽  
Long Term Storage ◽  
Pose Control ◽  
First Time

Botrytis cinerea causes gray mold and is an economically important postharvest pathogen of fruit, vegetables, and ornamentals. Fludioxonil-sensitive B. cinerea isolates were collected in 2011 and 2013 from commercial storage in Pennsylvania. Eight isolates had values for effective concentrations for inhibiting 50% of mycelial growth of 0.0004 to 0.0038 μg/ml for fludioxonil and were dual resistant to pyrimethanil and thiabendazole. Resistance was generated in vitro, following exposure to a sublethal dose of fludioxonil, in seven of eight dual-resistant B. cinerea isolates. Three vigorously growing B. cinerea isolates with multiresistance to postharvest fungicides were further characterized and found to be osmosensitive and retained resistance in the absence of selection pressure. A representative multiresistant B. cinerea strain caused decay on apple fruit treated with postharvest fungicides, which confirmed the in vitro results. The R632I mutation in the Mrr1 gene, associated with fludioxonil resistance in B. cinerea, was not detected in multipostharvest fungicide-resistant B. cinerea isolates, suggesting that the fungus may be using additional mechanisms to mediate resistance. Results from this study show for the first time that B. cinerea with dual resistance to pyrimethanil and thiabendazole can also rapidly develop resistance to fludioxonil, which may pose control challenges in the packinghouse environment and during long-term storage.

Yeasts from intertidal zone marine sediment demonstrate antagonistic activities against Botrytis cinerea in vitro and in strawberry fruit

2021 ◽  
pp. 104612
Author(s):  
Xiurong Zou ◽  
Yingying Wei ◽  
Kun Dai ◽  
Feng Xu ◽  
Hongfei Wang ◽  
...  
Keyword(s):  
Botrytis Cinerea ◽  
Marine Sediment ◽  
Intertidal Zone ◽  
Strawberry Fruit

Pathogenicity of postfire ascomycetes (Pezizales) on seeds and germinants of lodgepole pine

1986 ◽  
Vol 64 (10) ◽  
pp. 2368-2371 ◽  
Author(s):  
Keith N. Egger ◽  
J. W. Paden
Keyword(s):  
Seed Germination ◽  
Botrytis Cinerea ◽  
Pinus Contorta ◽  
Lodgepole Pine ◽  
Seed Infection

Forty isolates of postfire ascomycetes (Pezizales) were tested for in vitro pathogenicity on seeds and germinants of lodgepole pine, Pinus contorta Dougl. Two known pathogens, Caloscypha fulgens (Pers.) Boud. and Botrytis cinerea Pers., were included as a check of the method. Caloscypha fulgens was the only fungus that caused serious declines in seed germination, although several species of postfire Pezizales were capable of preemergence seed infection. Rhizina undulata Fr. and Pyropyxis rubra (Peck) Egger were strongly pathogenic on germinants. Pyropyxis rubra was not previously known to be pathogenic. Many isolates were weakly pathogenic on germinants under the conditions tested.

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