Successful orthotopic uterine allotransplantation in a rabbit model using aorta and cava anastomoses: a short-term viability study

This study is to investigate the effect of bisphosphonates on the osseointegration of dental implants in a rabbit model. Twenty female New Zealand White rabbits were equally assigned into control and experiment groups which received saline or zoledronic acid treatment 4 weeks prior to surgery. Titanium dental implant was placed on the calvarial bone. Zoledronic acid or saline treatment continued after surgery for 4 weeks (short-term subgroup) or 8 weeks (long-term subgroup) until sacrifice. Three different fluorochrome labeling solutions were administrated for assessing bone growth rates. Samples of the calvarial bone and mandible were subjected to microcomputed tomography (micro-CT), confocal microscope, and histology analysis. Zoledronic acid treatment significantly reduced bone growth rates in the calvarial bone, but had no significant influence in bone mineral density and trabecular microarchitecture. Significantly lower bone-to-implant contact ratios were found in zoledronic acid-treated animals compared to controls at week 4 but not at week 8. Oncologic dose zoledronic acid suppresses the bone growth rates of the calvarial bone; ZA may have an adverse effect on osseointegration of dental implant in short term, but this effect tends to diminish in long term.

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Earlier preterm birth is associated with a worse neurocognitive outcome in a rabbit model

PLoS ONE ◽

10.1371/journal.pone.0246008 ◽

2021 ◽

Vol 16 (1) ◽

pp. e0246008

Author(s):

Johannes van der Merwe ◽

Lennart van der Veeken ◽

Analisa Inversetti ◽

Angela Galgano ◽

Jaan Toelen ◽

...

Keyword(s):

Preterm Birth ◽

Rabbit Model ◽

Late Preterm ◽

Short Term ◽

Policy Makers ◽

Mortality And Morbidity ◽

Short Term Mortality ◽

Neurobehavioral Testing ◽

Postconceptional Age ◽

Cross Fostering

Background Preterm birth (PTB) and particularly late preterm PTB has become a research focus for obstetricians, perinatologists, neonatologists, pediatricians and policy makers alike. Translational models are useful tools to expedite and guide clinical but presently no model exists that contextualizes the late PTB scenario. Herein we aimed to develop a rabbit model that echo’s the clinical neurocognitive phenotypes of early and late PTB. Methods Time mated rabbit does underwent caesarean delivery at a postconceptional age (PCA) of either 28 (n = 6), 29 (n = 5), 30 (n = 4) or 31 (n = 4) days, term = 31 d. Newborn rabbits were mixed and randomly allocated to be raised by cross fostering and underwent short term neurobehavioral testing on corrected post-natal day 1. Open field (OFT), spontaneous alteration (TMT) and novel object recognition (NORT) tests were subsequently performed at 4 and 8 weeks of age. Results PTB was associated with a significant gradient of short-term mortality and morbidity inversely related to the PCA. On postnatal day 1 PTB was associated with a significant sensory deficit in all groups but a clear motor insult was only noted in the PCA 29d and PCA 28d groups. Furthermore, PCA 29d and PCA 28d rabbits had a persistent neurobehavioral deficit with less exploration and hyperanxious state in the OFT, less alternation in TMT and lower discriminatory index in the NORT. While PCA 30d rabbits had some anxiety behavior and lower spontaneous alteration at 4 weeks, however at 8 weeks only mild anxiety driven behavior was observed in some of these rabbits. Conclusions In this rabbit model, delivery at PCA 29d and PCA 28d mimics the clinical phenotype of early PTB while delivery at PCA 30d resembles that of late PTB. This could serve as a model to investigate perinatal insults during the early and late preterm period.

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Changes of Ocular Surface and the Inflammatory Response in a Rabbit Model of Short-Term Exposure Keratopathy

PLoS ONE ◽

10.1371/journal.pone.0137186 ◽

2015 ◽

Vol 10 (9) ◽

pp. e0137186 ◽

Cited By ~ 5

Author(s):

Chun-Ting Lai ◽

Wei-Chieng Yao ◽

Szu-Yuan Lin ◽

Hsin-Yu Liu ◽

Huai-Wen Chang ◽

...

Keyword(s):

Inflammatory Response ◽

Ocular Surface ◽

Rabbit Model ◽

Short Term ◽

Short Term Exposure

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Abstract 3035: Decrease Of Aortic Valve Area In A Hypercholesterolemic Rabbit Model Is Reversed By Short-term ApoAI-treatment

Circulation ◽

10.1161/circ.116.suppl_16.ii_680 ◽

2007 ◽

Vol 116 (suppl_16) ◽

Author(s):

Walter S Speidl ◽

Borja Ibanez ◽

Carlos G Santos-Gallego ◽

Mario J Garcia ◽

Valentin Fuster ◽

...

Keyword(s):

Aortic Valve ◽

Transthoracic Echocardiography ◽

Heart Surgery ◽

Rabbit Model ◽

Atherogenic Diet ◽

Mutant Form ◽

Aortic Valve Area ◽

Short Term ◽

Atherosclerotic Lesions ◽

Array Probe

Background. Aortic valve stenosis (AVS) is the second most common indication for heart surgery. Althoug recent observations linked AVS and atherosclerosis, medical treatment of AVS is limited. ApoAI-Milano (ApoAI-M) is a mutant form of ApoAI. We have recently shown that ApoAI-M rapidly regressed established atherosclerotic lesions in a hypercholesteremic rabbit model. The aim of this study was to test whether hypercholestermia decreases AVA in rabbits and whether this can be reversed by short-term ApoAI-M-treatment. Methods. We used transthoracic echocardiography (Philips HD 11 XE echograph with a 12-MHz phased-array probe) to compare AVA, as determined by continuity equation, of 14 white New Zeeland rabbits, fed with atherogenic diet (0.2 % of cholesterol) for nine month, with 6 healthy controls. The hypercholesterolemic animals were then randomized into ApoAI-M (ETC-216; Pfizer): 2 injections (75mg/Kg) in 4 days, or placebo. Six days after the last dose, we assessed aortic valve morphology by echocardiography and histopathology. Results. Hypercholesteremic diet resulted in a markedly reduced AVA compared to controls (0.17±0.02cm2 vs. 0.21±0.03cm2, p<0.01). ApoAI-M treatment increased AVA by 27 % (p<0.05) whereas placebo had no effect, resulting in significantly higher AVA in treated rabbits (0.22±0.03cm2 vs 0.16±0.02cm2, p<0.005). Histopathologic cross-examination showed markedly reduced thickness of aortic valve leaflets in ApoAI-M-treated rabbits. Conclusion. We show that reduction of AVA can be induced experimentally in hypercholesterolemic rabbits and can be investigated by transthoracic echocardiography. In addition, this study provides for the first time evidence that AVA can be increased pharmacologically, namely by treatment with ApoAI-M.

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