Maternal plasma and amniotic fluid dehydroepiandrosterone-sulfate concentrations in preterm labor and delivery

1996 ◽  
Vol 259 (1) ◽  
pp. 7-12
Author(s):  
M. Mazor ◽  
F. Ghezzi ◽  
J. Cohen ◽  
R. Hershkovitz ◽  
J. E. Tolosa ◽  
...  
Keyword(s):  
Amniotic Fluid ◽  
Preterm Labor ◽  
Dehydroepiandrosterone Sulfate ◽  
Maternal Plasma ◽  
Labor And Delivery

Maternal plasma and amniotic fluid dehydroepiandrosterone-sulfate concentrations in preterm labor and delivery

1996 ◽  
Vol 259 (1) ◽  
pp. 7-12 ◽  
Author(s):  
M. Mazor ◽  
F. Ghezzi ◽  
J. Cohen ◽  
R. Hershkovitz ◽  
J. E. Tolosa ◽  
...  
Keyword(s):  
Amniotic Fluid ◽  
Preterm Labor ◽  
Dehydroepiandrosterone Sulfate ◽  
Maternal Plasma ◽  
Labor And Delivery

Inflammatory Proteins in the Amniotic Fluid, Plasma, and Cervicovaginal Fluid for the Prediction of Intra-amniotic Infection/Inflammation and Imminent Preterm Birth in Preterm Labor

2020 ◽  
Author(s):  
Su A. Kim ◽  
Kyo H. Park ◽  
Seung M. Lee ◽  
Yu M. Kim ◽  
Subeen Hong
Keyword(s):  
Amniotic Fluid ◽  
Preterm Labor ◽  
Maternal Plasma ◽  
High Plasma ◽  
Clinical Parameters ◽  
Plasma Samples ◽  
Protein Levels ◽  
Inflammatory Protein ◽  
Associated Proteins ◽  
Cervicovaginal Fluid

Objective This study was aimed to develop models using multiple cytokine/chemokine levels in cervicovaginal fluid (CVF) and plasma and widely used noninvasive parameters that have better accuracy for predicting intra-amniotic infection and/or inflammation (IAI) and imminent spontaneous preterm delivery (SPTD, ≤48 hours) in women with preterm labor (PTL). Study Design This was a retrospective cohort study of 95 singleton pregnant women with PTL (23–34 weeks) who underwent amniocentesis. Both CVF and plasma samples were obtained at the time of amniocentesis, and serum C-reactive protein (CRP) levels were measured. The amniotic fluid (AF), CVF, and plasma samples were assayed for interleukin (IL)-6, IL-8, IL-10, monocyte chemotactic protein-1 (MCP-1), and macrophage inflammatory protein-1β (MIP-1β) levels using a multiplex immunoassay kit. Results The levels of most cytokines/chemokines measured in the AF and CVF were significantly higher in the women with than in those without IAI and imminent SPTD, whereas only high-plasma IL-10 level showed a significant association with imminent SPTD. In predicting IAI, proteins in AF had significantly higher areas under the curves (AUCs) than those in CVF and plasma. However, for predicting imminent SPTD, no significant differences in the AUCs of the outcome-associated proteins were observed among the measurements in AF, CVF, and maternal plasma. By using stepwise regression analyses, noninvasive models (using protein levels in CVF and baseline clinical parameters) were developed for the prediction of IAI and imminent SPTD. The AUC of these noninvasive models were similar to those of the invasive models (using AF protein levels and baseline clinical parameters). Conclusion Noninvasive models based on CVF cytokine/chemokine levels and widely used noninvasive parameters (especially CRP) act as good indicators for predicting the risk of IAI and imminent SPTD in women with PTL. Evaluation of cytokine/chemokine levels in plasma samples did not add valuable information regarding the two outcome measures in the PTL setting. Key Points

scholarly journals Chronic inflammatory lesions of the placenta are associated with an up-regulation of amniotic fluid CXCR3: A marker of allograft rejection

2018 ◽  
Vol 46 (2) ◽  
pp. 123-137 ◽  
Author(s):  
Eli Maymon ◽  
Roberto Romero ◽  
Gaurav Bhatti ◽  
Piya Chaemsaithong ◽  
Nardhy Gomez-Lopez ◽  
...  
Keyword(s):  
Amniotic Fluid ◽  
Allograft Rejection ◽  
Preterm Labor ◽  
Case Control Study ◽  
Case Control ◽  
Labor And Delivery ◽  
Retrospective Case ◽  
Inflammatory Lesions ◽  
Show Evidence ◽  
Control Study

AbstractObjective:The objective of this study is to determine whether the amniotic fluid (AF) concentration of soluble CXCR3 and its ligands CXCL9 and CXCL10 changes in patients whose placentas show evidence of chronic chorioamnionitis or other placental lesions consistent with maternal anti-fetal rejection.Methods:This retrospective case-control study included 425 women with (1) preterm delivery (n=92); (2) term in labor (n=68); and (3) term not in labor (n=265). Amniotic fluid CXCR3, CXCL9 and CXCL10 concentrations were determined by ELISA.Results:(1) Amniotic fluid concentrations of CXCR3 and its ligands CXCL9 and CXCL10 are higher in patients with preterm labor and maternal anti-fetal rejection lesions than in those without these lesions [CXCR3: preterm labor and delivery with maternal anti-fetal rejection placental lesions (median, 17.24 ng/mL; IQR, 6.79–26.68) vs. preterm labor and delivery without these placental lesions (median 8.79 ng/mL; IQR, 4.98–14.7; P=0.028)]; (2) patients with preterm labor and chronic chorioamnionitis had higher AF concentrations of CXCL9 and CXCL10, but not CXCR3, than those without this lesion [CXCR3: preterm labor with chronic chorioamnionitis (median, 17.02 ng/mL; IQR, 5.57–26.68) vs. preterm labor without chronic chorioamnionitis (median, 10.37 ng/mL; IQR 5.01–17.81; P=0.283)]; (3) patients with preterm labor had a significantly higher AF concentration of CXCR3 than those in labor at term regardless of the presence or absence of placental lesions.Conclusion:Our findings support a role for maternal anti-fetal rejection in a subset of patients with preterm labor.

A Rapid Amniotic Fluid Interleukin-6 Assessment for the Identification of Intra-Amniotic Inflammation in Women with Preterm Labor and Intact Membranes

2021 ◽  
pp. 1-6
Author(s):  
Teresa Cobo ◽  
Victoria Aldecoa ◽  
Magdalena Holeckova ◽  
Ctirad Andrys ◽  
Xavier Filella ◽  
...  
Keyword(s):  
Amniotic Fluid ◽  
Predictive Model ◽  
Predictive Value ◽  
Preterm Labor ◽  
Diagnostic Performance ◽  
Clinical Signs ◽  
Entire Study ◽  
Microbial Invasion ◽  
Automated Method ◽  
Spontaneous Delivery

<b><i>Objectives:</i></b> A multivariable predictive model has recently been developed with good accuracy to predict spontaneous preterm delivery within 7 days in women with preterm labor (PTL) and intact membranes. However, this model measures amniotic fluid (AF) interleukin (IL)-6 concentrations using the ELISA method, thereby limiting clinical implementation. The main objectives of this study were to validate the automated immunoassay as a quantitative method to measure AF IL-6 in women with PTL and to evaluate the diagnostic performance of AF IL-6 alone and as part of a multivariable predictive model to predict spontaneous delivery in 7 days with this automated method. <b><i>Study Design:</i></b> This is a retrospective observational study in women with PTL below 34 weeks who underwent amniocentesis to rule out microbial invasion of the amniotic cavity. Women with clinical signs of chorioamnionitis, cervical length measurement at admission &#x3e;5th centile, maternal age &#x3c;18 years, and no consent to perform amniocentesis for this indication were excluded. The local Institutional Review Boards approved the study (HCB/2019/0940). <b><i>Analysis of AF IL-6 Concentrations:</i></b> AF IL-6 concentrations were measured using an automated Cobas e602 electrochemiluminescence immunoanalyzer and Human IL-6 Quantikine ELISA kit. <b><i>Results:</i></b> Of the entire study group (<i>n</i> = 100), 38 women spontaneously delivered within 7 days after admission. Both laboratory methods showed good agreement (intraclass correlation coefficient: 0.937 (95% confidence interval [CI] 0.908–0.957); <i>p</i> &#x3c; 0.001). Diagnostic performance of AF IL-6 to predict spontaneous delivery within 7 days when it was included in the multivariable predictive model showed an area under the receiver operating characteristic curve of 0.894 (95% CI 0.799–0.955), sensitivity of 97%, specificity of 74%, positive predictive value of 73%, negative predictive value of 97%, positive likelihood ratio (LR) of 3.7, and negative LR of 0.045. <b><i>Conclusion:</i></b> While both analytical methods were comparable for measuring AF IL-6 concentrations in women with PTL, the Cobas immunoanalyzer provided rapid diagnosis of intra-amniotic inflammation within minutes. The predictive model showed a good diagnostic performance to target women at high risk of spontaneous delivery within 7 days.

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