Assessment of absorbed dose of gamma rays using the simultaneous determination of inactive hemoglobin derivatives as a biological dosimeter

2019 ◽  
Vol 59 (1) ◽  
pp. 131-144 ◽  
Author(s):  
A. M. M. Attia ◽  
W. M. Aboulthana ◽  
G. M. Hassan ◽  
E. Aboelezz
Keyword(s):  
Simultaneous Determination ◽  
Gamma Rays ◽  
Absorbed Dose ◽  
Hemoglobin Derivatives

Multicomponent analysis of hemoglobin derivatives with reversed-optics spectrophotometer.

1984 ◽  
Vol 30 (3) ◽  
pp. 373-379 ◽  
Author(s):  
A Zwart ◽  
A Buursma ◽  
E J van Kampen ◽  
W G Zijlstra
Keyword(s):  
Simultaneous Determination ◽  
Separate Determination ◽  
Overdetermined System ◽  
Chemical Research ◽  
Chemical Laboratory ◽  
Multicomponent Analysis ◽  
Hemoglobin Derivatives ◽  
Clinical Chemical ◽  
Human Blood Samples

Abstract We describe a method for the simultaneous determination of the five clinically relevant hemoglobin derivatives (Hb, HbO2, HbCO, Hi, SHb) in a blood sample by means of a reversed-optics spectrophotometer (Hewlett-Packard HP8450 A UV/Vis). A built-in computer program is used for multicomponent analysis in an overdetermined system, i.e., a system in which the number of independent equations used exceeds the number of unknowns to be determined. First, the spectra of the five hemoglobin derivatives are measured in a series of different human blood samples. Thereafter, the multicomponent method for the simultaneous determination of the five hemoglobin derivatives is tested by comparison with conventional methods for the separate determination of oxygen saturation, HbCO, Hi, and SHb fractions. The multicomponent (multiwavelength) method is sufficiently reliable, accurate, and easy to justify its use in physiological chemical research as well as its routine application in the clinical chemical laboratory.

scholarly journals Simultaneous determination of hemoglobin derivatives, oxygen content, oxygen capacity, and oxygen saturation in 10 microliters of whole blood.

1977 ◽  
Vol 23 (7) ◽  
pp. 1215-1225 ◽  
Author(s):  
L Rossi-Bernardi ◽  
M Perella ◽  
M Luzzana ◽  
M Samaja ◽  
I Raffaele
Keyword(s):  
Oxygen Saturation ◽  
Simultaneous Determination ◽  
Oxygen Content ◽  
Oxygen Pressure ◽  
Whole Blood ◽  
Sample Volume ◽  
New Method ◽  
Hemoglobin Derivatives ◽  
Oxygen Capacity

Abstract We describe a new method for simultaneous determination of four hemoglobin derivatives (deoxyhemoglobin, Hb; oxyhemoglobin, HbO2; methemoglobin, Hb+; and carbon monoxide hemoglobin, HbCO) and total oxygen content in 10 microliters of whole blood. Percentage HbO2, HbCO, Hb+, total hemoglobin (Hbt), and oxygen capacity can also be obtained from the experimental data by simple calculations. Total analysis time is 1 min. Blood is diluted 100-fold with a buffer contained in a quasi-anaerobic cuvette, where simultaneous measurements of oxygen pressure (by a po2 electrode) and adsorbance (at 497, 565, and 620 nm) are made. The decrease of oxygen pressure, as recorded by the oxygen electrode, is proportional to the amount of deoxyhemoglobin. The concentrations of HbO2, HbCO, and Hb+ can be obtained from absorbance measurements at the specified wavelengths. The new method eliminates the use of short-path optical cells and, due to the low sample volume requirement, makes possible the automated measurement of hemoglobin derivatives and oxygen saturation in arterialized capillary blood.

A general computer program for the extended plate overlap algorithm

1978 ◽  
Vol 48 ◽  
pp. 287-293 ◽  
Author(s):  
Chr. de Vegt ◽  
E. Ebner ◽  
K. von der Heide
Keyword(s):  
Computer Program ◽  
Simultaneous Determination ◽  
General Computer Program ◽  
General Computer

In contrast to the adjustment of single plates a block adjustment is a simultaneous determination of all unknowns associated with many overlapping plates (star positions and plate constants etc. ) by one large adjustment. This plate overlap technique was introduced by Eichhorn and reviewed by Googe et. al. The author now has developed a set of computer programmes which allows the adjustment of any set of contemporaneous overlapping plates. There is in principle no limit for the number of plates, the number of stars, the number of individual plate constants for each plate, and for the overlapping factor.

Sign in / Sign up

Export Citation Format

Share Document