In situ detection of the mast cell proteases chymase and tryptase in human lung tissue using light and electron microscopy

2001 ◽  
Vol 116 (6) ◽  
pp. 483-493 ◽  
Author(s):  
Waltraud J. Beil ◽  
Johannes Pammer
Keyword(s):  
Electron Microscopy ◽  
Mast Cell ◽  
Lung Tissue ◽  
Human Lung ◽  
Light And Electron Microscopy ◽  
Human Lung Tissue ◽  
In Situ Detection

Mast Cell Heterogeneity in Human Lung Tissue

1989 ◽  
Vol 77 (3) ◽  
pp. 297-304 ◽  
Author(s):  
F. J. Van Overveld ◽  
L. A. M. J. Houben ◽  
F. E. M. Schmitz du Moulin ◽  
P. L. B. Bruijnzeel ◽  
J. A. M. Raaijmakers ◽  
...  
Keyword(s):  
Mast Cell ◽  
Mast Cells ◽  
Alcian Blue ◽  
Lung Tissue ◽  
Human Lung ◽  
Immunoglobulin E ◽  
Prostaglandin D2 ◽  
Leukotriene C4 ◽  
Human Lung Tissue ◽  
No Release

1. In this study mast cells were found to comprise 2.1% of total cells recovered by enzymatic digestion of human lung tissue. 2. This mast cell population consisted of 79% formalin-sensitive, Alcian Blue-positive mast cells and 21% formalin-insensitive, Alcian Blue-positive mast cells. 3. By the use of centrifugal elutriation and subsequent Percoll gradient centrifugation, separate mixed cell populations could be obtained in which the mast cell constituents were either of the formalin-sensitive or -insensitive type. 4. Cell suspensions in which formalin-sensitive cells comprised 97% of mast cells contained approximately 1.34 pg of histamine per mast cell, whereas in preparations in which mast cells were 84% formalin-resistant the histamine content was approximately 4.17 pg of histamine per mast cell. 5. The histamine release upon anti-immunoglobulin E challenge of formalin-sensitive mast cells was greater than the release by formalin-insensitive mast cells. 6. After challenge with opsonized zymosan, only formalin-sensitive mast cells were able to release histamine. 7. Leukotriene C4 release was observed when formalin-sensitive mast cells were challenged with antiimmunoglobulin E. Formalin-insensitive mast cells showed no release of leukotriene C4. 8. Prostaglandin D2 release was observed when formalin-insensitive mast cells were challenged with antiimmunoglobulin E. Formalin-sensitive mast cells showed no release of prostaglandin D2.

scholarly journals A labelled-ubiquicidin antimicrobial peptide for immediate in situ optical detection of live bacteria in human alveolar lung tissue

2015 ◽  
Vol 6 (12) ◽  
pp. 6971-6979 ◽  
Author(s):  
Ahsan R. Akram ◽  
Nicolaos Avlonitis ◽  
Annamaria Lilienkampf ◽  
Ana M. Perez-Lopez ◽  
Neil McDonald ◽  
...  
Keyword(s):  
Antimicrobial Peptide ◽  
Lung Tissue ◽  
Human Lung ◽  
Optical Detection ◽  
Human Lung Tissue ◽  
Bacterial Detection ◽  
Live Bacteria

A fluorescently labelled ubiquicidin peptide enables bacterial detection in human lung tissuein vitro.

Electron microscopy analysis of mineral fibers in human lung tissue

1992 ◽  
Vol 22 (1) ◽  
pp. 49-58 ◽  
Author(s):  
Karl Heinz Friedrichs ◽  
Michael Brockmann ◽  
Margit Fischer ◽  
Gabriele Wick
Keyword(s):  
Electron Microscopy ◽  
Lung Tissue ◽  
Human Lung ◽  
Human Lung Tissue ◽  
Mineral Fibers ◽  
Electron Microscopy Analysis ◽  
Microscopy Analysis

Mast cell subtypes from human lung tissue: Their identification, separation, and functional characteristics

1988 ◽  
Vol 23 (3-4) ◽  
pp. 227-229 ◽  
Author(s):  
F. J. Overveld ◽  
L. A. M. J. Houben ◽  
P. L. B. Bruijnzeel ◽  
J. A. M. Raaijmakers ◽  
G. K. Terpstra ◽  
...  
Keyword(s):  
Mast Cell ◽  
Lung Tissue ◽  
Human Lung ◽  
Functional Characteristics ◽  
Human Lung Tissue

Some aspects of mast cell subtypes from human lung tissue

1990 ◽  
Vol 30 (1-2) ◽  
pp. 24-29 ◽  
Author(s):  
F. J. Overveld
Keyword(s):  
Mast Cell ◽  
Lung Tissue ◽  
Human Lung ◽  
Human Lung Tissue

scholarly journals Lung Immune Tone Regulation by the Gut-Lung Immune Axis: Short-chain Fatty Acid Receptors FFAR2 and FFAR3, and IL-1β Expression Profiling in Mouse and Human Lung

2020 ◽  
Author(s):  
Qing Liu ◽  
Xiaoli Tian ◽  
Daisuke Maruyama ◽  
Mehrdad Arjomandi ◽  
Arun Prakash
Keyword(s):  
Lung Tissue ◽  
Gut Microbiome ◽  
Human Lung ◽  
Expression Patterns ◽  
Metabolic Programming ◽  
Short Chain ◽  
Alveolar Type ◽  
Human Lung Tissue

ABSTRACTMicrobial metabolites produced by the gut microbiome, such as short-chain fatty acids (SCFA), can influence both local intestinal and distant lung physiology and response to injury. However, how lung immune activity is regulated by SCFAs is unknown. We examined fresh human lung tissue and observed the presence of SCFAs with large inter-individual and even intra-lobe variability. In vitro, SCFAs were capable of modifying the metabolic programming in both resting and LPS-exposed alveolar macrophages (AM). Additionally, since we hypothesized that lung immune tone could be defined through priming of the inflammasome (aka signal 1), we interrogated naïve mouse lungs for pro-IL-1β message and localized its presence within the alveolar space in situ, specifically in AM subsets, and in close proximity to alveolar type 2 epithelial (AT2) cells. We established that metabolically active gut microbiota, that produce SCFAs, can transmit LPS and SCFAs to the lung (potential sources of signal 1), and thereby could regulate lung immune tone and metabolic programming. To understand how murine lung cells sensed and upregulated IL-1β in response to gut-microbiome factors, we determined that in vitro, AM and AT2 cells expressed SCFA receptors, FFAR2, FFAR3, and IL-1β but with different expression patterns and LPS-inducibility. Finally, we observed that IL-1β, FFAR2 and FFAR3 were expressed both in isolated human AM and AT2 cells ex-vivo, but in fresh human lung sections in situ, only AM expressed IL-1β at rest and after LPS challenge. Together, this translational study using mouse and human lung tissue and cells supports an important role for the gut microbiome and SCFAs in regulating lung immune tone.

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