Immunohistochemical analysis of two stem cell markers of α-smooth muscle actin and STRO-1 during wound healing of human dental pulp

2012 ◽  
Vol 138 (4) ◽  
pp. 583-592 ◽  
Author(s):  
Nagako Yoshiba ◽  
Kunihiko Yoshiba ◽  
Naoto Ohkura ◽  
Yoshimi Shigetani ◽  
Erika Takei ◽  
...  
Keyword(s):  
Wound Healing ◽  
Smooth Muscle ◽  
Stem Cell ◽  
Dental Pulp ◽  
Smooth Muscle Actin ◽  
Immunohistochemical Analysis ◽  
Stem Cell Markers ◽  
Muscle Actin ◽  
Cell Markers ◽  
Human Dental Pulp

scholarly journals Comparison of Immuno-Phenotypes of Stem Cells from Human Dental Pulp and Periodontal Ligament

2012 ◽  
Vol 25 (1) ◽  
pp. 127-134 ◽  
Author(s):  
D. Ponnaiyan ◽  
K.M. Bhat ◽  
G.S. Bhat
Keyword(s):  
Stem Cells ◽  
Stem Cell ◽  
Cell Population ◽  
Dental Pulp ◽  
Periodontal Ligament ◽  
Embryonic Stem ◽  
Stem Cell Population ◽  
Stem Cell Markers ◽  
Cell Markers ◽  
Human Dental Pulp

It has been established that human dental pulp and periodontal ligament contain a population of mesenchymal stem cells (MSCs). However, the phenotypic analysis in terms of putative stem cell markers expressed by these stem cell populations is incomplete. It is relevant to understand whether stem cells derived from closely related tissues are programmed differently. The aim of the present study is to analyze whether these stem cells depict distinct characteristics by gaining insight into differences in their immunophenotype. Dental pulp and periodontal ligament tissue samples were obtained from extracted impacted wisdom teeth. Cell cultures were analyzed for surface and intracellular markers by indirect immunoflourescence. Detailed immunophenotype analysis was carried out by flow cytometry using relevant markers. The present study data shows dental pulp stem cells (DPSCs) and periodontal ligament stem cells (PDLSCs) expressed embryonic stem (ES) cell markers Oct-4, Nanog and mesodermal marker Vimentin by indirect immunoflourescence. PDLSCs, however, had a weak expression of Nanog. Immunophenotyping revealed strong expression of MSC markers (CD73, CD90) in DPSCs and PDLSCs. Differences were observed in expression of sternness-related markers. DPSCs displayed increased percentages of SSEA4, CD13 and CD166 and decreased CD9 expression compared to PDLSCs. Both stem cells express common MSC markers, different levels of expression suggests there might be more than one stem cell population existing within these tissues which differ in their embryonic status, and DPSCs are a more primitive stem cell population in comparison to PDLSCs.

scholarly journals Immunohistochemical Expression of Stem Cell Markers during the Wound Healing Process of Cutaneous Burn

2010 ◽  
Vol 79 (1) ◽  
pp. 1 ◽  
Author(s):  
Young Hee Choi ◽  
Min Gyu Kim ◽  
Dong-Hyun Ahn ◽  
Seong Jin Cho ◽  
Soo Hee Hong ◽  
...  
Keyword(s):  
Wound Healing ◽  
Stem Cell ◽  
Healing Process ◽  
Wound Healing Process ◽  
Stem Cell Markers ◽  
Immunohistochemical Expression ◽  
Cell Markers

scholarly journals Abalone Collagen Extracts Potentiate Stem Cell Properties of Human Epidermal Keratinocytes

Marine Drugs ◽  
2019 ◽  
Vol 17 (7) ◽  
pp. 424
Author(s):  
Sajee Thaweekitphathanaphakdee ◽  
Pithi Chanvorachote ◽  
Sagaw Prateepchinda ◽  
Mattaka Khongkow ◽  
Apirada Sucontphunt
Keyword(s):  
Cell Proliferation ◽  
Wound Healing ◽  
Stem Cell ◽  
Cell Migration ◽  
Expression Level ◽  
Epidermal Keratinocytes ◽  
Stem Cell Markers ◽  
Spheroid Formation ◽  
Human Epidermal Keratinocytes ◽  
Cell Markers

Stem cell activities in human tissues are critical for tissue integrity and function. Maintaining keratinocyte stem cells (KSCs) stemness helps sustain healthy skin by supporting keratinocyte renewal, involving the formation of epidermal barriers. In this study, abalone collagen (AC) extracts with molecular weights of 3 kDa (AC 1) and 300 kDa (AC 2) were compared to the epidermal growth factor (EGF) for their effects on cell proliferation, cell migration (wound healing), spheroid formation, and the expression level of stem cell markers on human keratinocytes (HaCaT cells). Cell viability was measured by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay and cell proliferation was quantified by ATP and DNA content analysis and Sulforhodamine B (SRB) assays. Cell migration assay was determined using the scratch wound healing test. Spheroid formation was evaluated and the expression level of stem cell markers was investigated by western blot analysis. The results showed that AC 1 at the concentration of 100 µg/mL could stimulate HaCaT cell proliferation, migration, spheroid formation, and the expression level of stem cell markers (keratin 19, β-catenin, ALDH1A1) compared to the control. In conclusion, a smaller molecular weight of abalone collagen extract exhibits a better effect on keratinocytes proliferation, migration, and stemness, which could be a potential active ingredient in cosmeceutical products.

α-Smooth-muscle Actin in and Contraction of Porcine Dental Pulp Cells

2002 ◽  
Vol 81 (3) ◽  
pp. 203-208
Author(s):  
D.P. Brock ◽  
R. Marty-Roix ◽  
M. Spector
Keyword(s):  
Smooth Muscle ◽  
Dental Pulp ◽  
Smooth Muscle Actin ◽  
Muscle Actin ◽  
Dental Pulp Cells ◽  
Pulp Cells

Expression of Multiple Stem Cell Markers in Dental Pulp Cells Cultured in Serum-free Media

2010 ◽  
Vol 36 (7) ◽  
pp. 1139-1144 ◽  
Author(s):  
Thais Miyuki Hirata ◽  
Nikolay Ishkitiev ◽  
Ken Yaegaki ◽  
Bogdan Calenic ◽  
Hiroshi Ishikawa ◽  
...  
Keyword(s):  
Stem Cell ◽  
Dental Pulp ◽  
Stem Cell Markers ◽  
Dental Pulp Cells ◽  
Serum Free ◽  
Cell Markers ◽  
Pulp Cells ◽  
Free Media ◽  
Cells Cultured
Sign in / Sign up

Export Citation Format

Share Document