Effects of prolonged voluntary wheel-running on muscle structure and function in rat skeletal muscle

2004 ◽  
Vol 92 (1-2) ◽  
pp. 90-97 ◽  
Author(s):  
Fumihiko Kariya ◽  
Hideki Yamauchi ◽  
Keizo Kobayashi ◽  
Mistuo Narusawa ◽  
Yoshibumi Nakahara
Keyword(s):  
Skeletal Muscle ◽  
Wheel Running ◽  
Structure And Function ◽  
Rat Skeletal Muscle ◽  
Voluntary Wheel Running ◽  
Muscle Structure ◽  
And Function ◽  
Voluntary Wheel

Deleterious effects of chronic clenbuterol treatment on endurance and sprint exercise performance in rats

2000 ◽  
Vol 98 (3) ◽  
pp. 339-347 ◽  
Author(s):  
Noel D. DUNCAN ◽  
David A. WILLIAMS ◽  
Gordon S. LYNCH
Keyword(s):  
Cardiac Muscle ◽  
Exercise Performance ◽  
Wheel Running ◽  
Structure And Function ◽  
Muscle Structure ◽  
Sprint Running ◽  
Heart Mass ◽  
Chronic Clenbuterol ◽  
And Function ◽  
Voluntary Wheel

The β2-adrenergic agonist, clenbuterol, has powerful muscle anabolic and lipolytic effects and is used by athletes to improve exercise performance; however, its use in conjunction with different forms of exercise training has received limited attention. Since previous studies have reported that chronic use of other β2-adrenergic agonists has deleterious effects on cardiac muscle structure and function, the aim of the present study was to determine whether chronic clenbuterol administration would reduce the exercise capabilities of rats subjected to long-term treadmill sprint running, endurance swimming or voluntary wheel running training. The effect of clenbuterol treatment on exercise performance in rats was evaluated in three separate studies. Different groups of male rats were assigned to an endurance swimming (2 h/day, 5/7 days, 18 weeks) group, a treadmill sprint running (8×1 min bouts, 1.05 m/s, 20 weeks) group, or a voluntary wheel running (16 weeks) group. In each study, rats were allocated into either a treated group that received clenbuterol (2 mg·kg-1·day-1) in their drinking water or an untreated control group. In each of the three studies, treated rats exhibited a reduction in exercise performance compared with untreated rats. Treated rats ran ∼ 57% less total distance than untreated rats in the voluntary running programme and were unable to complete the swimming and sprinting protocols performed by the untreated rats. In each of the studies, the treated rats exhibited cardiac hypertrophy, with absolute heart mass increased by ∼ 19% and heart mass relative to body mass increased by ∼ 20%. The hearts of sedentary rats treated with clenbuterol exhibited extensive collagen infiltration surrounding blood vessels and in the wall of the left ventricle. The results indicate strongly that chronic clenbuterol administration deleteriously affects exercise performance in rats, potentially due to alterations in cardiac muscle structure and function.

scholarly journals Essential Role of Septin 7 in Skeletal Muscle Structure and Function

2020 ◽  
Vol 118 (3) ◽  
pp. 258a
Author(s):  
Laszlo Csernoch ◽  
Mónika Gönczi ◽  
Zsolt Ráduly ◽  
László Szabó ◽  
Nóra Dobrosi ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Essential Role ◽  
Structure And Function ◽  
Muscle Structure ◽  
And Function

scholarly journals Voluntary Wheel Running Selectively Augments Insulin-Stimulated Vasodilation in Arterioles from White Skeletal Muscle of Insulin-Resistant Rats

2012 ◽  
Vol 19 (8) ◽  
pp. 729-738 ◽  
Author(s):  
Catherine R. Mikus ◽  
Bruno T. Roseguini ◽  
Grace M. Uptergrove ◽  
E. Matthew Morris ◽  
Randy Scott Rector ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Wheel Running ◽  
Voluntary Wheel Running ◽  
Insulin Resistant ◽  
Voluntary Wheel

scholarly journals Effects of Ovariectomy on Skeletal Muscle Structure and Function in Young Female Rats: Protective Effects of the Flavanol (−)‐Epicatechin

2020 ◽  
Vol 34 (S1) ◽  
pp. 1-1
Author(s):  
Viridiana Navarrrete ◽  
Marcos Ayala ◽  
Antonio Rodriguez ◽  
Francisco Villarreal ◽  
Israel Ramirez-Sanchez
Keyword(s):  
Skeletal Muscle ◽  
Young Female ◽  
Structure And Function ◽  
Female Rats ◽  
Protective Effects ◽  
Muscle Structure ◽  
And Function

scholarly journals Five months of voluntary wheel running downregulates skeletal muscle LINE-1 gene expression in rats

2019 ◽  
Vol 317 (6) ◽  
pp. C1313-C1323 ◽  
Author(s):  
Matthew A. Romero ◽  
Petey W. Mumford ◽  
Paul A. Roberson ◽  
Shelby C. Osburn ◽  
Hailey A. Parry ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Dna Methylation ◽  
Exercise Training ◽  
Mrna Expression ◽  
Wheel Running ◽  
Mobile Dna ◽  
Voluntary Wheel Running ◽  
L6 Myotubes ◽  
Voluntary Wheel

Transposable elements (TEs) are mobile DNA and constitute approximately half of the human genome. LINE-1 (L1) is the only active autonomous TE in the mammalian genome and has been implicated in a number of diseases as well as aging. We have previously reported that skeletal muscle L1 expression is lower following acute and chronic exercise training in humans. Herein, we used a rodent model of voluntary wheel running to determine whether long-term exercise training affects markers of skeletal muscle L1 regulation. Selectively bred high-running female Wistar rats ( n = 11 per group) were either given access to a running wheel (EX) or not (SED) at 5 wk of age, and these conditions were maintained until 27 wk of age. Thereafter, mixed gastrocnemius tissue was harvested and analyzed for L1 mRNA expression and DNA content along with other L1 regulation markers. We observed significantly ( P < 0.05) lower L1 mRNA expression, higher L1 DNA methylation, and less L1 DNA in accessible chromatin regions in EX versus SED rats. We followed these experiments with 3-h in vitro drug treatments in L6 myotubes to mimic transient exercise-specific signaling events. The AMP-activated protein kinase (AMPK) agonist 5-aminoimidazole-4-carboxamide ribonucleotide (AICAR; 4 mM) significantly decreased L1 mRNA expression in L6 myotubes. However, this effect was not facilitated through increased L1 DNA methylation. Collectively, these data suggest that long-term voluntary wheel running downregulates skeletal muscle L1 mRNA, and this may occur through chromatin modifications. Enhanced AMPK signaling with repetitive exercise bouts may also decrease L1 mRNA expression, although the mechanism of action remains unknown.

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