scholarly journals The (in)dependency of blood and sweat sodium, chloride, potassium, ammonia, lactate and glucose concentrations during submaximal exercise

2020 ◽  
Author(s):  
L. Klous ◽  
C. J. de Ruiter ◽  
S. Scherrer ◽  
N. Gerrett ◽  
H. A. M. Daanen
Keyword(s):  
Sodium Chloride ◽  
Submaximal Exercise ◽  
Blood Composition ◽  
Maximum Heart Rate ◽  
Post Exercise ◽  
Non Invasive ◽  
Potassium Lactate ◽  
Blood Potassium ◽  
Sweat Composition ◽  
Sweat Sodium

Abstract Purpose To reduce the need for invasive and expensive measures of human biomarkers, sweat is becoming increasingly popular in use as an alternative to blood. Therefore, the (in)dependency of blood and sweat composition has to be explored. Methods In an environmental chamber (33 °C, 65% relative humidity; RH), 12 participants completed three subsequent 20-min cycling stages to elicit three different local sweat rates (LSR) while aiming to limit changes in blood composition: at 60% of their maximum heart rate (HRmax), 70% HRmax and 80% HRmax, with 5 min of seated-rest in between. Sweat was collected from the arm and back during each stage and post-exercise. Blood was drawn from a superficial antecubital vein in the middle of each stage. Concentrations of sodium, chloride, potassium, ammonia, lactate and glucose were determined in blood plasma and sweat. Results With increasing exercise intensity, LSR, sweat sodium, chloride and glucose concentrations increased (P ≤ 0.026), while simultaneously limited changes in blood composition were elicited for these components (P ≥ 0.093). Sweat potassium, lactate and ammonia concentrations decreased (P ≤ 0.006), while blood potassium decreased (P = 0.003), and blood ammonia and lactate concentrations increased with higher exercise intensities (P = 0.005; P = 0.007, respectively). The vast majority of correlations between blood and sweat parameters were non-significant (P > 0.05), with few exceptions. Conclusion The data suggest that sweat composition is at least partly independent of blood composition. This has important consequences when targeting sweat as non-invasive alternative for blood measurements.

scholarly journals Increased gastric Pco 2 during exercise is indicative of gastric ischaemia: a tonometric study

Gut ◽  
1999 ◽  
Vol 44 (2) ◽  
pp. 163-167 ◽  
Author(s):  
J J Kolkman ◽  
A B J Groeneveld ◽  
F G van der Berg ◽  
J A Rauwerda ◽  
S G M Meuwissen
Keyword(s):  
Clinical Importance ◽  
Invasive Procedure ◽  
Normal Subjects ◽  
Submaximal Exercise ◽  
Peak Exercise ◽  
Gastric Tonometry ◽  
Revascularisation Procedure ◽  
Non Invasive ◽  
Angiographic Findings ◽  
Gastric Ischaemia

BackgroundDiagnosis of gastric ischaemia is difficult and angiography is an invasive procedure. Angiographic findings may not correlate with clinical importance.AimsTo investigate whether tonometric measurement of intragastric Pco2during exercise can be used to detect clinically important gastric ischaemia.MethodsFourteen patients with unexplained abdominal pain or weight loss were studied. Splanchnic angiography served as the gold standard. Three patients were studied again after a revascularisation procedure. Gastric Pco2 was measured from a nasogastric tonometer, with 10 minute dwell times, and after acid suppression. Gastric and capillary Pco2 were measured before, during, and after submaximal exercise of 10 minutes duration.ResultsSeven patients had normal angiograms; seven had more than 50% stenosis in the coeliac (n=7) or superior mesenteric artery (n=4). Normal subjects showed no changes in tonometry. In patients with stenoses, the median intragastric Pco2(Pico2) at rest was 5.2 kPa (range 4.8–11.2) and rose to 6.4 kPa (range 5.7–15.7) at peak exercise; the median intragastric blood Pco2 gradient increased from 0.0 kPa (range −0.8 to 5.9) to 1.7 kPa (range 0.9 to 10.3; p<0.01). Only two subjects had abnormal tonometry at rest; all had supernormal values at peak exercise. The Pco2 gradient correlated with clinical and gastroscopic severity; in patients reexamined after revascularisation (n=3), exercise tonometry returned to normal.ConclusionGastric tonometry during exercise is a promising non-invasive tool for diagnosing and grading gastrointestinal ischaemia and evaluating the results of revascularisation surgery for symptomatic gastric ischaemia.

Effects of age and exercise on inflammatory cytokines, HSP70 and HSP90 gene expression and protein content in Standardbred horses

2018 ◽  
Vol 14 (1) ◽  
pp. 27-46 ◽  
Author(s):  
R.C. Avenatti ◽  
K.H. McKeever ◽  
D.W. Horohov ◽  
K. Malinowski
Keyword(s):  
Gene Expression ◽  
Skeletal Muscle ◽  
Whole Blood ◽  
Plasma Cortisol ◽  
Acute Exercise ◽  
Submaximal Exercise ◽  
Hsp70 Expression ◽  
Post Exercise ◽  
Hsp90 Expression ◽  
Young Horses

We hypothesised that the cortisol response to acute exercise, markers of oxidative stress, expression of inflammatory cytokines, heat shock protein (HSP)70 and HSP90 expression in whole blood and skeletal muscle, and HSP70 and HSP90 protein concentrations in skeletal muscle are altered by age and in response to acute submaximal exercise in horses. Young (n=6; 5.5±2.8 year) and aged (n=6; 22.6±2.25 year) unconditioned Standardbred mares underwent an acute submaximal exercise test. Blood samples were collected and analysed for plasma cortisol and malondialdehyde (MDA) concentrations, and for cytokine and HSP gene expression pre- and post-exercise. Gluteus medius biopsies were obtained for analysis of cytokine and HSP gene expression pre- and at 0, 4, 24 and 48 h post-exercise. Data were analysed for main effects using a two-way ANOVA for repeated measures. Post-hoc comparisons of means were conducted using Student-Neuman-Keuls for pair-wise multiple comparisons where appropriate. Acute submaximal exercise increased plasma cortisol concentration in both young and aged mares, and the duration of the post-exercise rise in cortisol was altered in aged horses. Plasma MDA concentration and expression of tumour necrosis factor-α (TNF-α) and interleukin (IL)-6 were unchanged in blood and muscle. Exercise increased IL-1β expression in whole blood of young and aged mares, with young mares having greater exercise-induced expression at 2 (P<0.001) and 4 (P=0.019) h post-exercise. Both young and aged horses had increased HSP70 expression in whole blood following acute exercise, with young horses exhibiting 3-fold greater HSP70 expression than aged mares at 2 h post-exercise. HSP90 expression in whole blood following exercise was increased only in young horses. Both young and aged horses had increased HSP90 expression in skeletal muscle following exercise, but there was no difference due to age. However, the timing of HSP70 expression was different between young and aged horses. The age-related changes in cortisol and IL-1β expression following acute submaximal exercise can have implications for energy homeostasis and the adaption to such disturbances at a cellular and whole animal level. Quantification of HSP expression in whole blood may be a useful biomarker, with implications for cellular adaptation and survival in aged horses.

Non-Invasive Estimation of Hydration Status Changes through Tear Fluid Osmolarity during Exercise and Post-Exercise Rehydration

2014 ◽  
Vol 46 ◽  
pp. 272
Author(s):  
Corey T. Ungaro ◽  
Adam J. Reimel ◽  
Ryan P. Nuccio ◽  
Matthew D. Pahnke ◽  
Lindsay B. Baker
Keyword(s):  
Tear Fluid ◽  
Hydration Status ◽  
Post Exercise ◽  
Non Invasive

Skeletal muscle metabolic recovery following submaximal exercise in chronic heart failure is limited more by O2 delivery than O2 utilization

2010 ◽  
Vol 118 (3) ◽  
pp. 203-210 ◽  
Author(s):  
Hareld M.C. Kemps ◽  
Jeanine J. Prompers ◽  
Bart Wessels ◽  
Wouter R. De Vries ◽  
Maria L. Zonderland ◽  
...  
Keyword(s):  
Heart Failure ◽  
Skeletal Muscle ◽  
Chronic Heart Failure ◽  
Submaximal Exercise ◽  
Daily Activities ◽  
Control Group ◽  
Resonance Spectroscopy ◽  
Post Exercise ◽  
Metabolic Recovery ◽  
O2 Delivery

CHF (chronic heart failure) is associated with a prolonged recovery of skeletal muscle energy stores following submaximal exercise, limiting the ability to perform repetitive daily activities. However, the pathophysiological background of this impairment is not well established. The aim of the present study was to investigate whether muscle metabolic recovery following submaximal exercise in patients with CHF is limited by O2 delivery or O2 utilization. A total of 13 stable CHF patients (New York Heart Association classes II–III) and eight healthy subjects, matched for age and BMI (body mass index), were included. All subjects performed repetitive submaximal dynamic single leg extensions in the supine position. Post-exercise PCr (phosphocreatine) resynthesis was assessed by 31P-MRS (magnetic resonance spectroscopy). NIRS (near-IR spectroscopy) was applied simultaneously, using the rate of decrease in HHb (deoxygenated haemoglobin) as an index of post-exercise muscle re-oxygenation. As expected, PCr recovery was slower in CHF patients than in control subjects (time constant, 47±10 compared with 35±12 s respectively; P=0.04). HHb recovery kinetics were also prolonged in CHF patients (mean response time, 74±41 compared with 44±17 s respectively; P=0.04). In the patient group, HHb recovery kinetics were slower than PCr recovery kinetics (P=0.02), whereas no difference existed in the control group (P=0.32). In conclusion, prolonged metabolic recovery in CHF patients is associated with an even slower muscle tissue re-oxygenation, indicating a lower O2 delivery relative to metabolic demands. Therefore we postulate that the impaired ability to perform repetitive daily activities in these patients depends more on a reduced muscle blood flow than on limitations in O2 utilization.

scholarly journals Biological variation in sweat sodium chloride conductivity

2002 ◽  
Vol 39 (1) ◽  
pp. 39-43 ◽  
Author(s):  
D.-e. van der Merwe ◽  
J. B Ubbink ◽  
R. Delport ◽  
P. Becker ◽  
G. S Dhatt ◽  
...  
Keyword(s):  
Sodium Chloride ◽  
Biological Variation ◽  
Chloride Conductivity ◽  
Sweat Sodium

scholarly journals Reliability of the Urine Lactate Concentration After Alternating-Intensity Interval Exercise

Proceedings ◽  
2019 ◽  
Vol 25 (1) ◽  
pp. 1 ◽  
Author(s):  
Ioannis Kosmidis ◽  
Stefanos Nikolaidis ◽  
Alexandros Chatzis ◽  
Kosmas Christoulas ◽  
Thomas Metaxas ◽  
...  
Keyword(s):  
Blood Lactate ◽  
Lactate Concentration ◽  
Blood Lactate Concentration ◽  
Submaximal Exercise ◽  
Physically Active ◽  
Post Exercise ◽  
Interval Exercise ◽  
Average Value ◽  
Ad Libitum ◽  
Active Adults

Aim: Our previous studies have shown that the post-exercise urine lactate concentration is a reliable exercise biomarker under controlled post-exercise hydration conditions. However, the reliability of the urine lactate concentration has been examined only after brief maximal exercise. As a result, there is no information about the reliability of this biomarker after prolonged submaximal exercise. Thus, the aim of the present study was to examine the reliability of the urine lactate concentration after interval exercise of alternating intensity under controlled or ad libitum hydration during exercise. Material & Method: Twenty-eight physically active adults (16 men and 12 women) performed three identical 45-min running tests (2 sets of 22.5 min with 3 min rest interval) on the treadmill with alternating speed and inclination at 19–24 °C, spaced three days apart. The participants drank the same amount of water during exercise in two of tests and ad libitum in the other test, in random, counterbalanced order. Blood samples were collected before exercise and 1, 3, as well as 5 min post-exercise. The highest lactate value among the post-exercise samples of each individual was recorded as his/her peak post-exercise value. Urine samples were collected before exercise and 10 as well as 60 min post-exercise and the average value of the post-exercise samples was recorded. Blood and urine lactate were analyzed spectrophotometrically. Results: The peak post-exercise blood lactate concentration was 5.5 1.7 mmol/L (mean SD throughout) for men and 4.7 1.8 mmol/L for women. The post-exercise urine lactate concentration was 1.6 1.0 mmol/L for men and 1.5 1.0 mmol/L for women. The reliability of the blood lactate concentration at the three tests was high (ICC 077–0.88), being higher under controlled hydration. However, the reliability of the urine lactate concentration was low or non-significant (ICC 0.29–0.36). Conclusions: The urine lactate concentration after prolonged submaximal exercise was lower than the corresponding blood lactate concentration and showed unsatisfactory reliability regardless of the hydration pattern during exercise. Thus, it cannot be used as a biomarker for this kind of exercise.

Effect of differential music tempo on post-exercise cardiovascular recovery parameters in hypertensive individuals: a randomised control trial

2020 ◽  
pp. 1-8
Author(s):  
A.N. Siddiqui ◽  
J. Ganai ◽  
N. Khan ◽  
S. Davari ◽  
A. Mujaddadi
Keyword(s):  
Perceived Exertion ◽  
Heart Rate Recovery ◽  
Exercise Bout ◽  
Submaximal Exercise ◽  
Rating Of Perceived Exertion ◽  
Step Test ◽  
Randomised Control Trial ◽  
Cardiovascular Recovery ◽  
Post Exercise ◽  
Parasympathetic Reactivation

Hypertensive individuals tend to have autonomic dysfunction indicated by sympathetic dominance or delayed parasympathetic reactivation. A complimentary therapy such as music following exercise is considered to be beneficial in improving autonomic recovery. The purpose of this study was to assess the effect of differential music tempo on post-exercise cardiovascular recovery parameters in hypertensive individuals. Thirty hypertensive individuals were recruited for the present study which were randomly allocated to no music (n=10), slow music (n=10) and fast music (n=10) group. Participants in all three groups were subjected to submaximal exercise bout by Harvard step test. The cardiovascular recovery parameters i.e. heart rate recovery (HRR), blood pressure recovery (BPR) and rating of perceived exertion recovery (RPER) were assessed in all three groups after 1 min, 2 min and 3 min following termination of exercise. A significant decline was observed in HRR (P=0.002) and RPER (P=0.008) following exercise in slow music group as compared to fast and no music while no significant differences were observed in BPR between the three groups. The study concluded that music accelerates post-exercise recovery and slow music has greater effect as compared to fast or no music. These findings may have potential implications in the cardiovascular recovery dynamics in hypertensive individuals participating in submaximal exercise.

Abstract 16602: Pulmonary Vascular Response to Metaboreflex Stimulation During Submaximal Exercise in Heart Failure

Circulation ◽  
2014 ◽  
Vol 130 (suppl_2) ◽  
Author(s):  
Erik H VanIterson ◽  
Eric M Snyder ◽  
Bruce D Johnson ◽  
Thomas P Olson
Keyword(s):  
Heart Failure ◽  
Nyha Class ◽  
Submaximal Exercise ◽  
Afferent Feedback ◽  
Peak Oxygen Consumption ◽  
Pulmonary Vasculature ◽  
Baseline Trial ◽  
Post Exercise ◽  
Vascular Capacitance ◽  
Muscle Afferent

Introduction: Neural feedback from skeletal muscle during exercise contributes to changes in pulmonary pressures in healthy individuals. Heart failure patients (HF) often develop pulmonary hypertension; however, the relationship between muscle afferent feedback and the pulmonary vasculature in HF remains unclear. Hypothesis: We examined the influence of metaboreceptor stimulation on pulmonary vascular capacitance using a validated non-invasive gas exchange equivalent (GX CAP ) in HF. Methods: Eleven HF patients (age 51±5 yrs; EF, 32±3%; NYHA class, 1.6±0.2) and 11 controls (CTL; age 43±3 yrs) completed 3 cycling session (4-min at 60% of peak oxygen consumption, VO 2 ). Session one: baseline control trial. Sessions 2 and 3: bilateral upper-thigh tourniquets inflated suprasystolic for 2 min at end-exercise (regional circulatory occlusion, RCO) with or without addition of inspired CO 2 to maintain end-exercise end-tidal CO 2 (P ET CO 2 ) (RCO+CO 2 ) (randomized). Rest, exercise, and recovery heart rate (HR), P ET CO 2 , and VO 2 were measured. O 2 pulse (VO 2 /HR) and GX CAP (O 2 pulseхP ET CO 2 ) were calculated. Results: During all conditions at end-exercise, HF demonstrated significantly lower GX CAP compared to CTL (p<0.01). Percent change in GX CAP from end-exercise to 2 min post-exercise was attenuated in HF compared to CTL (41±5% vs 64±1%, respectively, p<0.01) during the baseline trial. During RCO, HF had a 55±6% reduction in GX CAP from end-exercise compared to 77±2% in CTL (p<0.01). During RCO+CO 2 , HF had a 49±4% reduction in GX CAP from end-exercise compared to 69±2% in CTL (p<0.01). GX CAP was similar between sessions within HF. The CTL group demonstrated an attenuated return of GX CAP during RCO compared to both baseline and RCO+CO 2 (p<0.01) with no difference between baseline and RCO+CO 2 . Conclusion: These data suggest the exercise mediated rise and post-exercise recovery of pulmonary vascular capacitance are attenuated in HF during constant-load submaximal exercise compared to CTL. Additionally, our data confirm previous reports that locomotor muscle afferent feedback influences pulmonary vascular capacitance in CTL; however, this model of locomotor muscle metaboreflex stimulation appears to a differential response in HF compared to CTL.

Sign in / Sign up

Export Citation Format

Share Document