Adrenal transplant for pernicious anemia. Prof. M. I. Arinkin (Vesti. Khir., Book 19)

Prof. MI Arinkin (Vesti. Khir., Book 19) describes 2 cases where such a transplant resulted in a significant improvement in the patients' well-being, the disappearance of weakness and dizziness and some improvement in blood composition.

Protective effect of Majoon Muṣaffī-i-Khūn (MMK) in cyclophosphamide-induced thrombocytopenia in male Sprague Dawley rats

Aim of the study: To evaluate the efficacy of Majoon Muṣaffī-i-Khūn (MMK) in cyclophosphamide-induced thrombocytopenia in male Sprague Dawley rats. Material and Methods: Thrombocytopenia was induced by administration of Cyclophosphamide (25 mg/kg, 3 days, SC). Simultaneously, the animals were treated with MMK (300 and 600 mg/kg) orally and continued for three weeks. After completion of treatment, blood samples were collected by retro-orbital puncture and analyzed for WBC, RBC, Hb, and platelet counts, with bleeding and clotting time. MMK insignificantly increased the platelets, WBC, RBC, and Hb with a significant decreased in bleeding (p<0.001; p<0.05) and clotting times (P<0.01, P<0.001) which was altered by cyclophosphamide. Results: The results demonstrated that MMK improved platelet counts, clotting, and bleeding time as well as restored the altered level of WBC, RBC, and Hb. Conclusion: Based on the above results it can be concluded that the hydroalcoholic extract of MMK may have the potential to normalize the blood composition effectively.

Leukocyte blood composition of Naturix natrix (Serpentes: Colubridae) in the Mordovian State Nature Reserve (Russia)

The state of the grass snake (Natrix natrix) population in the Mordovian State Nature Reserve was assessed using the hematological approach. The leukocyte blood count was estimated together with calculation of the associated leukocyte indices (neutrophil:lymphocyte (N:L), eosinophil:lymphocyte (E:L), heterophil:eosinophil (H/E) and heterophil:lymphocyte (H:L) ratios). The surface area of leukocytes (heterophils, basophils, eosinophils, azurophils, monocytes, and lymphocytes) was measured. The leukocyte blood composition of Natrix natrix was characterized by the predominance of agranulocytes, and the granulocyte fraction was 31– 37% (u = 1.99, p = 0.04). The population pattern of the leukocyte blood composition of Natrix natrix was as follows: heterophils – 8.07±0.6%, basophils – 12.33±0.95%, eosinophils – 8.33±0.65%, azurophils – 5.25±0.53%, monocytes – 9.77±0.42%, and lymphocytes – 56.22±1.7%. According to the averaged area, the leukocytes were arranged in the following order: eosinophils – monocytes – azurophils, basophils – heterophils – lymphocytes. The diameter of the largest cells (eosinophils) was (17.5±2.42) μm. The smallest lymphocytes had diameters within (7.22±1.21) μm. The absence of any differences in the quantitative content of all types of granulocytes and agranulocytes in the blood of the grass snake from different parts of the reserve indicated a comparable level of impact and the identity of the physiological mechanisms of adaptation that occur in the body of animals in protected habitat conditions. Most of the leukocyte integral indices revealed the same type of variability in the parameters of the white blood cell system of the Natrix natrix in three areas of the reserve, with the exception of an increased value of the heterophiles/lymphocytes ratio in the sample from the village Pushta. The increase in this indicator made it possible to draw a conclusion about the stress effect on the individuals of this sample. The principal component method was used to differentiate the samples of the grass snake with a more complete quantitative description of the leukocyte composition of the objects of study; the results obtained are presented in a visual, integrated and generalized form. The use of the principal component method made it possible to combine samples of Natrix natrix with similar indicators of leukocyte composition, as well as to identify a population of Natrix natrix that differs in its indicators in the urbanized territory of the Samara region. The blood pattern and the dynamics of blood leukocyte indices reflected the active response of the Natrix natrix body to the complex of environmental factors of the habitat on the territory of the Mordovian State Nature Reserve.

Effect of Mucuna Pruriens (Velvet Bean) Leaf Meal on Performance and Blood Composition of Finisher Broiler Chickens.

The effect of Mucuma pruriens (Velvet bean) leaf neul on the performance and blood composition of finisher broiler chickens were determined in a 35-day feeding trial. Chemical analysis indicated that the leaf meal is relutively high in crude protein (22.34%) and with estimated energy content of 1:108.93kJ/kg DM. Maize based diets containing mucuna leaf meul (MLM) at 0,, 5,. 7.5 and 10% levels, respectively were formulated and fed to finisher broilers in a complete randomized design replicuted four times. Luch replicate contained 10 birds. Birds fed MLM diets had a statistically (P>0.05) similar feed intake values with the control group, Body weight gain of the birds compared favorably with the control except at 10% (P<0.05) dietary inclusion level. Birds fed 10% MLM dict hud the lowest weight gain. Feed conversion ratios were similar among the 1), 5 and 7.5% diel groups. The blood compositions of finisher broilers in terins of packed cell volume, red blood cell and haemoglohin increased with increasing dietary level of the meal but the values were not significantly (P>0.05) different from the control. With increasing dietary inclusion levels of MLM in the diets, the erythrocytic and leucocytic (WBC, lymphoecytas and neutrophils) parameters varied without any negative significant (P>0.05) effects. It is concluded that 7.5% MLM could be used in broiler finisher diets without any deleterious effects on the performance Enhanced erythrocytes values were obtained at 10% dietary level.