Fungal elicitor Pep-25 increases cytosolic calcium ions, H2O2 production and activates the octadecanoid pathway in Arabidopsis thaliana

AbstractHydrogen peroxide (H2O2) production during the infection of Arabidopsis thaliana by the soybean cyst nematode Heterodera glycines was detected histochemically by the reaction of H2O2 with cerium chloride producing four different patterns of electron-dense precipitates of cerium perhydroxides. As A. thaliana is not a regular host of H. glycines, the defence response is considerable, but does not completely inhibit the development of the nematode. H2O2 was produced not only by cells mechanically damaged during invasion and feeding site induction by the nematode, but also by cells surrounding developing syncytia and cells which were neither in contact with the nematode nor with the syncytium. Die Lokalisation von Peroxid wahrend der Abwehrreaktion von Arabidopsis thaliana gegen den pflanzenparasitaren Nematoden Heterodera glycines - Die Bildung von Wasserstoffperoxid (H2O2) im Rahmen der Infektion von Arabidopsis thaliana durch den Sojabohnen-Zystennematoden Heterodera glycines wurde histochemisch durch die Reaktion von H2O2 mit Cerchlorid nachgewiesen, wobei vier verschiedene Muster elektronendichter Prazipitate von Cerperhydroxiden gebildet wurden. Da A. thaliana kein regularer Wirt von H. glycines ist, kommt es zu einer betrachtlichen Abwehrreaktion, die jedoch die Entwicklung des Nematoden nicht vollstandig verhindert. H2O2 wurde nicht nur von Zellen produziert, die im Laufe des Eindringens und der Induktion des Nahrzellensystems durch den Nematoden mechanisch beschadigt worden waren, sondern auch von Zellen, die sich entwickelnde Syncytien umgaben und von Zellen, die weder mit dem Nematoden noch mit dem Syncytium in Kontakt standen.

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Analytically depicting the calcium diffusion for Alzheimer’s affected cell

International Journal of Biomathematics ◽

10.1142/s1793524518500882 ◽

2018 ◽

Vol 11 (07) ◽

pp. 1850088 ◽

Cited By ~ 3

Author(s):

Devanshi D. Dave ◽

Brajesh Kumar Jha

Keyword(s):

Mathematical Model ◽

Calcium Ions ◽

Complex Structure ◽

Calcium Signalling ◽

Cytosolic Calcium ◽

Free Calcium ◽

Calcium Diffusion ◽

The Impact ◽

The Brain ◽

Very High

Brain is the most complex structure of the human body. The processes going inside the brain and the mechanisms behind it have been unrevealed up to certain extent only. Out of the various physiological phenomena carried out by the brain, calcium signalling can be considered as one of the most important. Calcium being a second messenger plays an important role in transformation of various information. In view of above, an attempt has been made here to study calcium signalling in presence of buffers, i.e. one kind of proteins and endoplasmic reticulum (ER), which is also known as store house of the cell. Being the store house of the cell, it has very high amount of calcium, whereas buffers decrease the level of free calcium ions by binding calcium ions to it. A two-dimensional mathematical model has been developed to see the impact of these parameters on cytosolic calcium concentration. This mathematical model is solved analytically using Laplace transforms and similarity transforms. The simulations are carried out using MATLAB. It is observed that the impact of buffer and ER is significant on calcium signalling. The obtained results are interpreted with the Alzheimeric condition of the nerve cells.

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Leaf Age-Dependent Effects of Foliar-Sprayed CuZn Nanoparticles on Photosynthetic Efficiency and ROS Generation in Arabidopsis thaliana

Materials ◽

10.3390/ma12152498 ◽

2019 ◽

Vol 12 (15) ◽

pp. 2498 ◽

Cited By ~ 6

Author(s):

Sperdouli ◽

Moustaka ◽

Antonoglou ◽

Adamakis ◽

Dendrinou-Samara ◽

...

Keyword(s):

Arabidopsis Thaliana ◽

Photosynthetic Efficiency ◽

Leaf Age ◽

Foliar Spray ◽

Ros Generation ◽

H2o2 Production ◽

Mature Leaves ◽

Transmission Electron ◽

Psii Photochemistry ◽

Young Leaves

Young and mature leaves of Arabidopsis thaliana were exposed by foliar spray to 30 mg L−1 of CuZn nanoparticles (NPs). The NPs were synthesized by a microwave-assisted polyol process and characterized by dynamic light scattering (DLS), X-ray diffraction (XRD), and transmission electron microscopy (TEM). CuZn NPs effects in Arabidopsis leaves were evaluated by chlorophyll fluorescence imaging analysis that revealed spatiotemporal heterogeneity of the quantum efficiency of PSII photochemistry (ΦPSΙΙ) and the redox state of the plastoquinone (PQ) pool (qp), measured 30 min, 90 min, 180 min, and 240 min after spraying. Photosystem II (PSII) function in young leaves was observed to be negatively influenced, especially 30 min after spraying, at which point increased H2O2 generation was correlated to the lower oxidized state of the PQ pool.. Recovery of young leaves photosynthetic efficiency appeared only after 240 min of NPs spray when also the level of ROS accumulation was similar to control leaves. On the contrary, a beneficial effect on PSII function in mature leaves after 30 min of the CuZn NPs spray was observed, with increased ΦPSΙΙ, an increased electron transport rate (ETR), decreased singlet oxygen (1O2) formation, and H2O2 production at the same level of control leaves.An explanation for this differential response is suggested.

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Involvement of the Octadecanoid Pathway and Protein Phosphorylation in Fungal Elicitor-Induced Expression of Terpenoid Indole Alkaloid Biosynthetic Genes in Catharanthus roseus

PLANT PHYSIOLOGY ◽

10.1104/pp.119.4.1289 ◽

1999 ◽

Vol 119 (4) ◽

pp. 1289-1296 ◽

Cited By ~ 155

Author(s):

Frank L.H. Menke ◽

Stefanie Parchmann ◽

Martin J. Mueller ◽

Jan W. Kijne ◽

Johan Memelink

Keyword(s):

Protein Phosphorylation ◽

Catharanthus Roseus ◽

Indole Alkaloid ◽

Fungal Elicitor ◽

Terpenoid Indole Alkaloid ◽

Biosynthetic Genes ◽

Induced Expression ◽

Octadecanoid Pathway

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The alteration of mitotic events by ionophore A23187 and carbonyl cyanide n-chlorophenylhydrazone

Journal of Cell Science ◽

10.1242/jcs.75.1.347 ◽

1985 ◽

Vol 75 (1) ◽

pp. 347-355

Author(s):

M.L. Ziegler ◽

J.E. Sisken ◽

S. Vedbrat

Keyword(s):

Oxidative Phosphorylation ◽

Intracellular Calcium ◽

Calcium Ions ◽

Hela Cells ◽

Cytosolic Calcium ◽

Ionophore A23187 ◽

Chromosome Movement ◽

Low Concentrations ◽

Carbonyl Cyanide ◽

Stimulation Of

A large quantity of published work indicates that calcium ions may be involved in the regulation of mitotic events and recent reports suggest that the onset of chromosome movement is dependent upon a transient increase in free cytosolic calcium ions. In this paper we examine the effects of two agents known to perturb intracellular calcium pools on mitosis in HeLa cells. These were the calcium-selective ionophore A23187 and carbonyl cyanide n-chlorophenylhydrazone (CCCP), which is a protonophoric inhibitor of oxidative phosphorylation. Owing to a stimulation of glycolysis, the latter agent does not decrease intracellular ATP in HeLa but does cause mitochondria to release calcium ions. Our data show that, at low concentrations, both agents prolong metaphase but differ in their effects on anaphase and cytokinesis. Studies with chlorotetracycline, a commonly used probe for membrane-associated calcium, verify that these agents do affect calcium pools under the conditions of our experiments. The data presented are consistent with the idea that increased cytosolic calcium levels can directly or indirectly affect mitotic events but, contrary to other suggestions, cause a prolongation of metaphase, i.e. they delay the onset of chromosome movement.

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The Octadecanoid Pathway, but Not COI1, Is Required for Nectar Secretion in Arabidopsis thaliana

Frontiers in Plant Science ◽

10.3389/fpls.2018.01060 ◽

2018 ◽

Vol 9 ◽

Cited By ~ 8

Author(s):

Anthony J. Schmitt ◽

Rahul Roy ◽

Peter M. Klinkenberg ◽

Mengyuan Jia ◽

Clay J. Carter

Keyword(s):

Arabidopsis Thaliana ◽

Nectar Secretion ◽

Octadecanoid Pathway

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Effects of Ca2+ and Mg2+ on ATP-dependent 45Ca2+ influx in A-431 human epidermoidal carcinoma cells

Biochemistry and Cell Biology ◽

10.1139/o91-004 ◽

1991 ◽

Vol 69 (1) ◽

pp. 29-35 ◽

Cited By ~ 2

Author(s):

Kenji Sugita ◽

Kazuo Hosoi ◽

Yoshimi Shioda ◽

Takao Ueha

Keyword(s):

Calcium Ions ◽

Calcium Influx ◽

Inositol Trisphosphate ◽

Cytosolic Calcium ◽

Carcinoma Cells ◽

Dependent Manner ◽

Parallel Effect ◽

Intracellular Calcium Ion ◽

Preincubation Medium ◽

In Cells

Upon stimulation with 10−6–10−3 M ATP, A-431 human epidermoidal carcinoma cells incorporated radioactive calcium from their medium in a temperature-dependent manner. The rate of incorporation of 45Ca2+ was rapid for the initial 5 min, but decreased immediately thereafter. The preincubation of cells for 2 h in medium depleted of both Ca2+ and Mg2+ abolished the ATP-dependent 45Ca2+ incorporation, irrespective of whether or not the subsequent incubation medium contained Mg2+ ions. ATP-dependent 45Ca2+ incorporation could be restored by a second preincubation (1 h) in medium containing 1 mM Mg2+, but no Ca2+. The Mg2+ ions in the second preincubation medium could be replaced by Ca2+, Co2+, or Cu2+ for restoration of such activity. Elevation of inositol trisphosphate (InsP3) was observed in cells depleted of either Ca2+ or Mg2+, but not in cells depleted of both ions. A parallel effect was observed in changes in [Ca2+]i. Since the concentration of cytosolic calcium ions does not change by incubation of cells in medium depleted of and (or) restored with calcium ions, we conclude that either calcium or magnesium ions associated with some cellular component(s) are responsible for production of InsP3, which then supposedly mobilizes Ca2+ and provokes 45Ca2+ influx.Key words: intracellular calcium ion, inositol trisphosphate, calcium influx.

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