scholarly journals Impact of Exogenous Elicitors on Artemisinin Production and Trichome Density in Artemisia annua L. under Subtropical Conditions

2014 ◽  
Vol 6 (3) ◽  
pp. 349-353 ◽  
Author(s):  
Alka Jagdish DANGASH ◽  
Neeta PANDYA ◽  
Ashish BHARILLYA ◽  
Ashween JHALA ◽  
Dharamchand JAIN
Keyword(s):  
Salicylic Acid ◽  
Gibberellic Acid ◽  
Indole Acetic Acid ◽  
Artemisia Annua ◽  
Glandular Trichomes ◽  
Acetyl Salicylic Acid ◽  
Trichome Density ◽  
Factors Affecting ◽  
Medicinal Properties ◽  
Artemisinin Content

Artemisinin is a sesquiterpene lactone found in Artemisia annua having many medicinal properties. Therefore the factors affecting trichome initiation are important. In the present study, several strategies have been adapted to increase artemisinin production by treating them with nutrients, hormones and elicitors. Abscisic acid, Gibberellic acid (GA3), a combination of GA3+IAA (Gibberellic acid+indole acetic acid), Chitosan, Methyl jasmonate, Acetyl salicylic acid, Lead acetate, sodium chloride, and Nanozime were sprayed on plant at regular intervals. The glandular trichomes of leaves from Artemisia annua (Asteraceae) were examined by light and scanning electron microscopy and the percentage artemisnin content was analyzed by HPTLC. Maximum artemisinin content % and trichome index (0.128) was found when methy jasmonate was applied and minimum artemisinin content % and trichome index was reported in NaCl & acetyl salicylic acid.

scholarly journals AaCycTL Regulates Cuticle and Trichome Development in Arabidopsis and Artemisia annua L.

2021 ◽  
Vol 12 ◽  
Author(s):  
Boran Dong ◽  
Xingxing Wang ◽  
Rui Jiang ◽  
Shiyuan Fang ◽  
Jinxing Li ◽  
...  
Keyword(s):  
Artemisia Annua ◽  
Glandular Trichome ◽  
Biosynthesis Pathway ◽  
Trichome Density ◽  
Trichome Development ◽  
Pathway Gene ◽  
Chinese Traditional Herb ◽  
Artemisinin Biosynthesis ◽  
Artemisinin Content ◽  
Important Drug

Artemisinin is an important drug for resistance against malaria. Artemisinin is derived from the glandular trichome of leaves, stems, or buds of the Chinese traditional herb Artemisia annua. Increasing the trichome density may enhance the artemisinin content of A. annua. It has been proven that cyclins are involved in the development of trichomes in tomato, Arabidopsis, and tobacco, but it is unclear whether the cyclins in A. annua influence trichome development. In this study, we showed that AaCycTL may regulate trichome development and affect the content of artemisinin. We cloned AaCycTL and found that it has the same expression files as the artemisinin biosynthesis pathway gene. We overexpressed AaCycTL in Arabidopsis, and the results indicated that AaCycTL changed the wax coverage on the surface of Arabidopsis leaves. The trichome density decreased as well. Using yeast two-hybrid and BiFC assays, we show that AaCycTL can interact with AaTAR1. Moreover, we overexpressed AaCycTL in A. annua and found that the expression of AaCycTL was increased to 82–195%. Changes in wax coverage on the surface of transgenic A. annua leaves or stems were found as well. We identified the expression of the artemisinin biosynthesis pathway genes ADS, CYP71AV1, and ALDH1 has decreased to 88–98%, 76–97%, and 82–97% in the AaCycTL-overexpressing A. annua lines, respectively. Furthermore, we found reduced the content of artemisinin. In agreement, overexpression of AaCycTL in A. annua or Arabidopsis may alter waxy loading, change the initiation of trichomes and downregulate trichome density. Altogether, AaCycTL mediates trichome development in A. annua and thus may serve to regulate trichome density and be used for artemisinin biosynthesis.

scholarly journals COMPARISON OF VARIOUS CHEMICAL TREATMENTS TO BREAK DORMANCY OF GLADIOLUS CORMS

2021 ◽  
pp. 1-9
Author(s):  
Yasar Sajjad
Keyword(s):  
Acetic Acid ◽  
Salicylic Acid ◽  
Gibberellic Acid ◽  
Growth Regulators ◽  
Indole Acetic Acid ◽  
Potassium Nitrate ◽  
Chemical Treatments ◽  
Dark Conditions ◽  
Short Time

Gladiolus is commercially propagated through corms and occurrence of dormancy in freshly harvested corms creates hindrance in their cultivation. Present study was designed to compare the effect of different chemicals to alleviate the dormancy of corms in a short time. Gladiolus corms were collected from two growing sites and subjected to chemicals methanol, benzylaminopurine (BAP), gibberellic acid (GA3), indole acetic acid (IAA), salicylic acid, potassium nitrate and thiourea at different concentrations alone or in combinations. The treated corms were planted on sand and placed under dark conditions at 26°C. The treatment of 60% methanol in combination with 0.9 mM BAP showed maximum germination (72.71%). Descaled corms showed 85% germination compared to 60.42% scaled corms, while the corms collected from Faisalabad showed better germination 83.75% compared to corms from Rawalakot (61.67%). The application of 60% methanol also induced 29.65% and 42.43% deterioration in scaled and descaled corms, respectively. The treatments of 0.3 mM BAP in combination with 0.6 mM GA3 exhibited 88.33 % germination in corms collected from Faisalabad as compared to 55% in corms from Rawalakot. Overall, application of BAP and GA3 in combination was found effective in shortening the period of dormancy in both cultivars of gladiolus compared to other tested chemicals or growth regulators.

scholarly journals Enhancing artemisinin content in and delivery from Artemisia annua: a review of alternative, classical, and transgenic approaches

Planta ◽  
2021 ◽  
Vol 254 (2) ◽  
Author(s):  
Kaiser Iqbal Wani ◽  
Sadaf Choudhary ◽  
Andleeb Zehra ◽  
M. Naeem ◽  
Pamela Weathers ◽  
...  
Keyword(s):  
Artemisia Annua ◽  
Artemisinin Content

scholarly journals The physiological response of Artemisia annua L. to salt stress and salicylic acid treatment

2014 ◽  
Vol 20 (2) ◽  
pp. 161-169 ◽  
Author(s):  
Lin Li ◽  
Haihui Zhang ◽  
Li Zhang ◽  
Yonghong Zhou ◽  
Ruiwu Yang ◽  
...  
Keyword(s):  
Salicylic Acid ◽  
Salt Stress ◽  
Physiological Response ◽  
Acid Treatment ◽  
Artemisia Annua ◽  
Salicylic Acid Treatment ◽  
Artemisia Annua L

Template bleeding time after ingestion of ultra low dosages of acetyl salicylic acid in healthy subjects. Preliminary study

1987 ◽  
Vol 48 (4) ◽  
pp. 501-504 ◽  
Author(s):  
C. Doutremepuich ◽  
D. Pailley ◽  
M.C. Anne ◽  
O. de Séze ◽  
J. Paccalin ◽  
...  
Keyword(s):  
Salicylic Acid ◽  
Healthy Subjects ◽  
Bleeding Time ◽  
Acetyl Salicylic Acid ◽  
Preliminary Study

Acetyl salicylic acid alleviates chilling-induced damage in muskmelon seedlings

2007 ◽  
Vol 87 (3) ◽  
pp. 581-585 ◽  
Author(s):  
Ahmet Korkmaz ◽  
Murat Uzunlu ◽  
Ali Riza Demirkiran
Keyword(s):  
Salicylic Acid ◽  
Cucumis Melo ◽  
Chilling Stress ◽  
Foliar Spray ◽  
Dry Weight ◽  
Acetyl Salicylic Acid ◽  
Signal Molecule ◽  
Stress Indicator ◽  
Shoot Dry Weight ◽  
Significant Difference

Salicylic acid (SA) is a common plant-produced signal molecule that is responsible for inducing tolerance to a number of biotic and abiotic stresses. An experiment was, therefore, conducted to test whether acetyl salicylic acid (ASA) application at various concentrations through seed immersion or foliar spray would protect muskmelon [Cucumis melo L. (Reticulatus Group)] seedlings subjected to chilling stress. Twenty-one-day-old plants pre-treated with ASA (0, 0.1, 0.25, 0.50 or 1.0 mM) were subjected to chilling stress for 72 h at 3 ± 0.5°C. ASA, applied either through seed immersion or foliar spray, was effective within the range of 0.1 to 1 mM in inducing tolerance to chilling stress in muskmelon seedlings; however, there was no significant difference between application methods. ASA significantly and curvilinearly affected all seedling growth and stress indicator variables tested except shoot dry weight. The best protection was obtained from seedlings pre-treated with 0.5 mM ASA. The highest ASA concentration used was slightly less effective in providing chilling stress protection. Even though both methods provided similar means of protection, due to its simplicity and practicality, immersion of muskmelon seeds prior to sowing in 0.5 mM ASA would be a more desirable method to induce tolerance to chilling stress. Key words: Cucumis melo, aspirin, chilling stress tolerance, gas exchange, electrolyte leakage

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