Correlation among agronomic, physiological and phytochemical traits in Artemisia annua L. varieties

The aim of this study was to estimate the correlations among agronomic, physiological and phytochemical traits of two varieties of Artemisia annua and to identify correlations that can be used in selection and breeding processes for this species. The design was completely randomized and the treatments were for Artemisia annua varieties 2/39x5x3M and 2/39x1V, which were subjected to agronomic, physiological and phytochemical evaluations. The relationship among the studied traits was estimated with Pearson's correlation coefficient, and the significance level of correlations was established by the t-test using Genes software. A significant positive correlation was found between the traits canopy volume and essential oil yield for 2/39x5x3M variety, showing that the selection of plants with a larger canopy volume would facilitate indirect selection of the trait essential oil yield. This correlation was not found for the 2/39x1V variety. In both varieties, there was a significant negative correlation between canopy volume and artemisinin content, which suggested that the selection of plants with a large canopy volume and a large number of branches should be avoided if the goal is to increase artemisinin content.

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Arbuscular Mycorrhizal Fungus Rhizophagus irregularis Influences Artemisia annua Plant Parameters and Artemisinin Content under Different Soil Types and Cultivation Methods

Microorganisms ◽

10.3390/microorganisms8060899 ◽

2020 ◽

Vol 8 (6) ◽

pp. 899

Author(s):

Erzsébet Domokos ◽

Béla Bíró-Janka ◽

János Bálint ◽

Katalin Molnár ◽

Csaba Fazakas ◽

...

Keyword(s):

Essential Oil ◽

Open Field ◽

Artemisia Annua ◽

Arbuscular Mycorrhizal Fungus ◽

Mycorrhizal Fungus ◽

Arbuscular Mycorrhizal ◽

Glandular Trichome ◽

Soil Types ◽

Essential Oil Yield ◽

Artemisinin Content

Artemisinin extracted from Artemisia annua has been used efficiently in malaria treatment since 2005. In this study, the variations in plant parameters (plant biomass, glandular trichome density, essential oil total chemical content, artemisinin production, and polyphenol oxidase (PPO) activity) were tested under different soil types (Luvisol, Gleysol, Anthrosol and sterile peat) and cultivation conditions (potted plants in semi-open field, and open field experiments) for plants inoculated with arbuscular mycorrhizal fungus (AMF) Rizophagus irregularis. Under semi-open field conditions, the AMF colonization of A. annua plant roots varied, and presented the highest percentage in Luvisol and sterile peat. The increase in the root colonization rate positively influenced some plant parameters (biomass, glandular trichome density, artemisinin concentration, essential oil quantity and composition), but no effects on PPO enzyme activity were detected. AMF fungus R. irregularis significantly increased the artemisinin content and essential oil yield of plants cultivated in Luvisol, Gleysol, Anthrosol and in peat. These soil types can offer appropriate conditions for A. annua cultivation and artemisinin production even on a smaller scale. Under open field conditions, low (about 5%) AMF colonization was observed. No differences in artemisin contents were detected, but essential oil yield significantly increased compared to control plants. AMF treatment increased beta-farnesene and germacrene D concentrations in Artemisia plants in the open field experiment.

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Distillation Time Effect on Essential Oil Yield, Composition, and Antioxidant Capacity of Sweet Sagewort (Artemisia annua L.) Oil

HortScience ◽

10.21273/hortsci.48.10.1288 ◽

2013 ◽

Vol 48 (10) ◽

pp. 1288-1292 ◽

Cited By ~ 9

Author(s):

Valtcho D. Zheljazkov ◽

Tess Astatkie ◽

Thomas Horgan ◽

Vicki Schlegel ◽

Xavier Simonnet

Keyword(s):

Essential Oil ◽

Antioxidant Capacity ◽

Oil Content ◽

Artemisia Annua ◽

Oil Yield ◽

Specific Chemical ◽

Alpha Pinene ◽

Sweet Wormwood ◽

Essential Oil Yield ◽

Reporting Data

Sweet sagewort, also known as sweet wormwood (Artemisia annua L.), contains essential oil and other natural products. The objective of this study was to evaluate the effect of eight different distillation times (DTs; 1.25 minutes, 2.5 minutes, 5 minutes, 10 minutes, 20 minutes, 40 minutes, 80 minutes, and 160 minutes) on A. annua essential oil and its antioxidant capacity. Highest essential oil yield was achieved at 160-minute DT. The concentration of camphor (8.7% to 50% in the oil) was highest at the shorter DT and reached a minimum at 160-minute DT. The concentration of borneol showed a similar trend as the concentration of camphor. The concentrations of some constituents in the oil were highest at 2.5-minute DT (alpha-pinene and camphene), at 10 minutes (paracymene), at 20 minutes (beta-chamigrene and gamma-himachalene), at 80 minutes [transmuurola-4(15),5-diene and spathulenol], at 80- to 160-minute DT (caryophylene oxide and cis-cadin-4-en-ol), or at 160-minute DT (beta-caryophyllene, transbeta-farnesene, and germacrene-D). The yield of individual constituents reached maximum at 20- to 160-minute DT (camphor) at 80- to 160-minute DT [paracymene, borneol, transmuurola-4(15),5-diene, and spathulenol], or at 160-minute DT (for the rest of the oil constituents). DT can be used to attain A. annua essential oil with differential and possibly targeted specific chemical profile. The highest antioxidant capacity of the oil was obtained at 20-minute DT and the lowest from the oil in the 5-minute DT. This study suggests that literature reports on essential oil content and composition of A. annua could be compared only if the essential oil was extracted at similar DTs. Therefore, DT must be reported when reporting data on essential oil content and composition of A. annua.

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CHARACTERISTICS FOR THE SELECTION OF PARENTS OF Corymbia citriodora AIMING TO THE PRODUCTION OF WOOD AND ESSENTIAL OIL

Revista Árvore ◽

10.1590/1806-90882018000100018 ◽

2018 ◽

Vol 42 (1) ◽

Author(s):

Otávio dos Santos Limeira Luz ◽

Ana Beatriz Marques Honório ◽

Rodrigo Ribeiro Fidelis ◽

Ildon Rodrigues do Nascimento ◽

Cristiano Bueno de Moraes ◽

...

Keyword(s):

Essential Oil ◽

Oil Production ◽

Oil Yield ◽

Leaf Nitrogen Content ◽

Correlation Degree ◽

Genetic Dissimilarity ◽

Corymbia Citriodora ◽

Essential Oil Yield ◽

Essential Oil Production ◽

Selection Of

ABSTRACT This study aimed to identify the most important characteristics for the selection of parent trees of Corymbia citriodora by estimating genetic dissimilarity and linear correlation degree for wood and essential oil production. This study was conducted in experimental areas of the company JAMP Florestal -located in Dueré-TO -and at Universidade Federal do Tocantins (Federal University of Tocantins), campus Gurupi, from January to April 2014, in 19 parent trees based on 19 characteristics. With the data, the following steps were performed: clustering through Tocher’s optimization method; calculation of genetic dissimilarity between parents; quantification of relative contribution; and verification of correlation degree existing among the characteristics evaluated through Pearson correlation using the program GENES. The characteristics essential oil yield and heartwood diameter presented the major contributions for genetic dissimilarity. Thus, it must be prioritized for wood production: trees with lower values in crown height and sapwood diameter; and trees with higher values in total height, commercial height, diameter at breast height and stump diameter. For essential oil production, it must be prioritized trees with lower values in leaf width, bark thickness and sapwood diameter; and trees with higher values in leaf nitrogen content, leaf potassium content, total green biomass and total dry biomass, essential oil yield, essential oil content and crown volume.

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EFFECT OF DIFFERENT ABIOTIC STRESS ON ESSENTIAL OIL YIELD FROM AERIAL PART OF CYMBOPOGON FLEXUOSUS (NEES EX STEUD) WATS.

International Journal of Advanced Research ◽

10.21474/ijar01/2549 ◽

2016 ◽

Vol 4 (12) ◽

pp. 1490-1504

Author(s):

Vinutha M ◽

◽

Suchetha M. ◽

K.J.Thara Saraswathi. ◽

◽

...

Keyword(s):

Abiotic Stress ◽

Essential Oil ◽

Aerial Part ◽

Oil Yield ◽

Cymbopogon Flexuosus ◽

Essential Oil Yield

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Identification of VOCs in essential oils extracted using ultrasound- and microwave-assisted methods from sweet cherry flower

Scientific Reports ◽

10.1038/s41598-020-80891-0 ◽

2021 ◽

Vol 11 (1) ◽

Author(s):

Huimin Zhang ◽

Hongguang Yan ◽

Quan Li ◽

Hui Lin ◽

Xiaopeng Wen

Keyword(s):

Essential Oil ◽

Sweet Cherry ◽

Dimethyl Sulfide ◽

Solid Phase ◽

Oil Yield ◽

Gas Chromatography Mass Spectrometry ◽

Process Conditions ◽

Important Indicator ◽

Microwave Assisted ◽

Essential Oil Yield

AbstractThe floral fragrance of plants is an important indicator in their evaluation. The aroma of sweet cherry flowers is mainly derived from their essential oil. In this study, based on the results of a single-factor experiment, a Box–Behnken design was adopted for ultrasound- and microwave-assisted extraction of essential oil from sweet cherry flowers of the Brooks cultivar. With the objective of extracting the maximum essential oil yield (w/w), the optimal extraction process conditions were a liquid–solid ratio of 52 mL g−1, an extraction time of 27 min, and a microwave power of 435 W. The essential oil yield was 1.23%, which was close to the theoretical prediction. The volatile organic compounds (VOCs) of the sweet cherry flowers of four cultivars (Brooks, Black Pearl, Tieton and Summit) were identified via headspace solid phase microextraction (SPME) and gas chromatography–mass spectrometry (GC–MS). The results showed that a total of 155 VOCs were identified and classified in the essential oil from sweet cherry flowers of four cultivars, 65 of which were shared among the cultivars. The highest contents of VOCs were aldehydes, alcohols, ketones and esters. Ethanol, linalool, lilac alcohol, acetaldehyde, (E)-2-hexenal, benzaldehyde and dimethyl sulfide were the major volatiles, which were mainly responsible for the characteristic aroma of sweet cherry flowers. It was concluded that the VOCs of sweet cherry flowers were qualitatively similar; however, relative content differences were observed in the four cultivars. This study provides a theoretical basis for the metabolism and regulation of the VOCs of sweet cherry flowers.

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Essential Oils of Four Virginia Mountain Mint (Pycnanthemum virginianum) Varieties Grown in North Alabama

Plants ◽

10.3390/plants10071397 ◽

2021 ◽

Vol 10 (7) ◽

pp. 1397

Author(s):

William N. Setzer ◽

Lam Duong ◽

Trang Pham ◽

Ambika Poudel ◽

Cuong Nguyen ◽

...

Keyword(s):

Essential Oil ◽

Essential Oils ◽

University Research ◽

Oil Yield ◽

Research Station ◽

Continuous Extraction ◽

Chromatographic Techniques ◽

Relative Differences ◽

Essential Oil Yield ◽

Very High

Virginia mountain mint (Pycnanthemum virginianum) is a peppermint-flavored aromatic herb of the Lamiaceae and is mainly used for culinary, medicinal, aromatic, and ornamental purposes. North Alabama’s climate is conducive to growing mint for essential oils used in culinary, confectionery, and medicinal purposes. There is, however, a need for varieties of P. virginianum that can be adapted and easily grown for production in North Alabama. Towards this end, four field-grown varieties with three harvesting times (M1H1, M1H2, M1H3; M2H1, M2H2, M2H3; M3H1, M3H2, M3H3, M4H1, M4H2, M4H3) were evaluated for relative differences in essential oil yield and composition. Thirty-day-old greenhouse-grown plants of the four varieties were transplanted on raised beds in the field at the Alabama A & M University Research Station in North Alabama. The plots were arranged in a randomized complete block with three replications. The study’s objective was to compare the four varieties for essential oil yield and their composition at three harvest times, 135, 155, and 170 days after planting (DAP). Essential oils were obtained by hydrodistillation with continuous extraction with dichloromethane using a Likens–Nickerson apparatus and analyzed by gas chromatographic techniques. At the first harvest, the essential oil yield of the four varieties showed that M1H1 had a yield of 1.15%, higher than M2H1, M3H1, and M4H1 with 0.91, 0.76, and 1.03%, respectively. The isomenthone concentrations increased dramatically through the season in M1 (M1H1, M1H2, M1H3) by 19.93, 54.7, and 69.31%, and M3 (M3H1, M3H2, M3H3) by 1.81, 48.02, and 65.83%, respectively. However, it increased only slightly in M2 and M4. The thymol concentration decreased slightly but not significantly in all four varieties; the thymol in M2 and M4 was very high compared with M1 and M3. The study showed that mountain mint offers potential for production in North Alabama. Two varieties, M1 and M3, merit further studies to determine yield stability, essential oil yield, composition, and cultivation development practices.

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