Extractable activities and protein content of sucrose-phosphate synthase, sucrose synthase and neutral invertase in trunk tissues of Robinia pseudoacacia L. are related to cambial wood production and heartwood formation

Planta ◽  
1998 ◽  
Vol 207 (2) ◽  
pp. 266-274 ◽  
Author(s):  
Siegfried Hauch ◽  
Elisabeth Magel
Keyword(s):  
Protein Content ◽  
Sucrose Synthase ◽  
Robinia Pseudoacacia ◽  
Sucrose Phosphate Synthase ◽  
Wood Production ◽  
Heartwood Formation ◽  
Neutral Invertase ◽  
Robinia Pseudoacacia L ◽  
Sucrose Phosphate ◽  
Phosphate Synthase

Carbohydrate Storage and Mobilisation by the Culm of Wheat Between Heading and Grain Maturity: the Relation to Sucrose Synthase and Sucrose-Phosphate Synthase

1994 ◽  
Vol 21 (3) ◽  
pp. 255 ◽  
Author(s):  
IF Wardlaw ◽  
J Willenbrink
Keyword(s):  
Sucrose Synthase ◽  
Flag Leaf ◽  
Sucrose Phosphate Synthase ◽  
Leaf Sheath ◽  
Water Soluble ◽  
Soluble Carbohydrates ◽  
Carbohydrate Storage ◽  
Stem Reserves ◽  
Sucrose Phosphate ◽  
Phosphate Synthase

Wheat plants grown under non-stress conditions at a dayhight temperature of 18/13�C under glasshouse conditions from head emergence to maturity showed a maximum accumulation of water-soluble, non-structural carbohydrates 20-25 days after anthesis. This storage was largely as fructans with the timing and amount of storage and mobilisation varying between cultivars, although the maximum concentration (fructose equivalents per unit stem fresh weight) was similar in all cultivars. The main storage in the culm was located in the lower part of the peduncle enclosed by the flag leaf sheath, in the penultimate internode and for one cultivar also in the flag leaf sheath. 14CO2 pulse-chase studies showed that there was a considerable delay in the incorporation of flag leaf assimilates into stem fructans, a delay probably associated with transfer and metabolic processes in the stem itself. At anthesis, when soluble carbohydrates were rapidly accumulating in the culm, the level of activity of sucrose synthase (SS) in the penultimate internode was much greater than that of sucrose phosphate synthase (SPS). The activity of SS declined rapidly as active storage ceased. This pattern was the reverse of that found in the leaf where SPS, rather than SS, was initially high and declined towards maturity. These changes are discussed in relation to the possible role of sucrose synthesising enzymes, particularly SS, in the accumulation and mobilisation of stem reserves in wheat.

Changes in Carbohydrate Content and the Activities of Acid invertase, Sucrose Synthase and Sucrose Phosphate Synthase in Asparagus Spears During Storage

2000 ◽  
Vol 4 (1) ◽  
pp. 94-97
Author(s):  
Pankaj Kumar Bhowmik ◽  
Toshiyuki Matsui ◽  
Fabio Gimena Enriquez ◽  
A.K.M. Shameem Alam ◽  
Kazuhide Kawada
Keyword(s):  
Sucrose Synthase ◽  
Sucrose Phosphate Synthase ◽  
Acid Invertase ◽  
Carbohydrate Content ◽  
Sucrose Phosphate ◽  
Phosphate Synthase

Sucrose phosphate synthase and sucrose synthase activity during maturation of internodal tissue in sugarcane

2000 ◽  
Vol 27 (1) ◽  
pp. 81 ◽  
Author(s):  
Frederik C. Botha ◽  
Kevin G. Black
Keyword(s):  
Sucrose Synthase ◽  
Accumulation Rate ◽  
Sucrose Phosphate Synthase ◽  
Sucrose Accumulation ◽  
Sucrose Content ◽  
Synthesis Activity ◽  
Species Hybrids ◽  
High Sucrose ◽  
Sucrose Phosphate ◽  
Phosphate Synthase

Sucrose accumulation rates, sucrose-phosphate synthase (SPS, EC 2.4.1.14) and soluble sucrose synthase (SuSy, EC 2.4.1.13) activities were measured in internodal tissue from a sugarcane (Saccharum species hybrids) variety N19. The sucrose accumulation rate sharply increases between internodes 3 to 11. In the older internodes SPS activity was at least three times higher than the SuSy activity. A highly significant positive correlation was found between SPS activity and sucrose content. In contrast, no significant correlation was observed between SuSy and sucrose content. In agreement, when radiolabelled glucose was fed to internodes with a high sucrose accumulation rate, label was equally distributed in the hexose moieties of sucrose. This clearly indicates that SPS is the major sucrose synthesis activity in the culm of sugarcane. Different kinetic forms of SPS apparently exist in the internodal tissue at different stages of development.

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