Telomerase activity significantly correlates with cell differentiation, proliferation and lymph node metastasis in colorectal carcinomas

Currently, a number of promising strategies and approaches to cancer treatment include differentiation therapy. However, theoretical and methodological foundations of this field are not yet well developed. The objective of this study was to determine the effects of a mixture of polyclonal activators (PAs; phytohaemagglutinin, concanavalin A and lipopolysaccharide) on cytokine production by biopsy samples of invasive breast carcinoma of no special type (IBC-NST) having various differentiation abilities and metastatic potentials as well as on differentiation status of the IBC-NST biopsy samples. We used ELISAs to investigate spontaneous and PA-stimulated cytokine production in the IBC-NST biopsy samples; from these data, we calculated a cytokine production stimulation index (SIPA). The effect of PAs on tumour cell differentiation was determined via a differentiation stimulation index (DSI). DSI was found to vary within the range 1.0–5.0. After treatment with PAs, in the IBC-NST biopsy samples of group I (DSI <1.25), the production of IL-2, IL-6, IL-8, IL-17, IL-18, IL-1β, IL-1Ra, TNF-α and GM-CSF increased; in the biopsy samples of group II (DSI >1.25), the production of IL-6, IL-1β, IL-1Ra, TNF-α, G-CSF and GM-CSF significantly increased, while the production of VEGF-A decreased. Receiver operating characteristic (ROC) analysis of SIPA revealed that increased production of IL-18 in the IBC-NST biopsy samples after exposure to PAs may block the PA-driven, cytokine-mediated differentiation of moderately differentiated into highly differentiated tumour cells. The ROC analysis also uncovered an association between the responses of tumour cells to PAs and lymph node metastasis observed in the patients. The findings suggest that there is a need for research aimed at finding new drugs for differentiating cancer therapy and at searching for targeted inducers of cytokine production or specific suppressors of their induction.

Download Full-text

Predictive factors for lymph node metastasis in t1 stage colorectal Carcinomas

Diseases of the Colon & Rectum ◽

10.1007/bf02660767 ◽

2003 ◽

Vol 46 (12) ◽

pp. 1626-1632 ◽

Cited By ~ 75

Author(s):

Masako Sakuragi ◽

Kazutomo Togashi ◽

Fumio Konishi ◽

Koji Koinuma ◽

Yutaka Kawamura ◽

...

Keyword(s):

Lymph Node ◽

Lymph Node Metastasis ◽

Predictive Factors ◽

Colorectal Carcinomas ◽

Node Metastasis ◽

T1 Stage

Download Full-text

Tu1490 Is the Depth of Submucosal Invasion a Significant Risk Factor for Lymph Node Metastasis in Submucosal-Invasive Colorectal Carcinomas?

Gastrointestinal Endoscopy ◽

10.1016/j.gie.2014.02.936 ◽

2014 ◽

Vol 79 (5) ◽

pp. AB559-AB560

Author(s):

Yuta Kouyama ◽

Shin-Ei Kudo ◽

Hideyuki Miyachi ◽

Katsuro Ichimasa ◽

Shingo Matsudaira ◽

...

Keyword(s):

Risk Factor ◽

Lymph Node ◽

Lymph Node Metastasis ◽

Significant Risk ◽

Significant Risk Factor ◽

Submucosal Invasion ◽

Colorectal Carcinomas ◽

Node Metastasis

Download Full-text

Predictive power of tumour budding for lymph node metastasis in colorectal carcinomas: A retrospective study

The Indian Journal of Medical Research ◽

10.4103/ijmr.ijmr_1268_17 ◽

2019 ◽

Vol 150 (6) ◽

pp. 635

Author(s):

Brototo Deb ◽

SajiniElizabeth Jacob

Keyword(s):

Lymph Node ◽

Retrospective Study ◽

Lymph Node Metastasis ◽

Predictive Power ◽

Colorectal Carcinomas ◽

Node Metastasis ◽

Tumour Budding

Download Full-text

Coexpression of MUC1 with p53 or MUC2 correlates with Lymph Node Metastasis in Colorectal Carcinomas

Journal of Korean Medical Science ◽

10.3346/jkms.2002.17.1.29 ◽

2002 ◽

Vol 17 (1) ◽

pp. 29 ◽

Cited By ~ 13

Author(s):

Kee Taek Jang ◽

Seoung Wan Chae ◽

Jin Hee Sohn ◽

Hye Rim Park ◽

Hyung Sik Shin

Keyword(s):

Lymph Node ◽

Lymph Node Metastasis ◽

Colorectal Carcinomas ◽

Node Metastasis

Download Full-text

Clinical Usefulness of Telomerase Assay for the Detection of Lymph Node Metastasis in Patients With Oral Malignancy

Archives of Pathology & Laboratory Medicine ◽

10.5858/2000-124-0398-cuotaf ◽

2000 ◽

Vol 124 (3) ◽

pp. 398-400

Author(s):

Tomoki Sumida ◽

Hiroyuki Hamakawa ◽

Hiroaki Kayahara ◽

Hirofumi Zen ◽

Kenichi Sogawa ◽

...

Keyword(s):

Lymph Node ◽

Lymph Node Metastasis ◽

Oral Cancer ◽

Lymph Nodes ◽

Cancer Cell Line ◽

Telomerase Activity ◽

Cervical Lymph Nodes ◽

Node Metastasis ◽

Significant Difference ◽

Oral Malignancy

Abstract Objective.—Telomerase is considered a diagnostic marker of malignancy. We investigated the usefulness of telomerase assay for the detection of lymph node micrometastasis. Methods.—Sixteen cervical lymph nodes with metastasis of oral cancer and 20 benign lymph nodes were studied. The oral cancer cell line was used to estimate the sensitivity for telomerase assay. Telomerase activity was measured by semiquantitative telomeric repeat amplification protocol. Results.—There was a significant difference between malignant and benign lymph nodes. The telomerase activity of 50 mg of lymph nodes with 103 or more cancer cells differed from that of control lymph nodes. Lymph nodes with 102 or fewer tumor cells expressed similar levels as benign lymph nodes. Conclusions.—In addition to routine histologic examination, telomerase assay is considered a useful tool for the detection of lymph node metastasis in patients with oral malignancy.

Download Full-text

Indication for Endoscopic Resection of Submucosal Colorectal Carcinoma: Special Reference to Lymph Node Metastasis

Diagnostic and Therapeutic Endoscopy ◽

10.1155/dte.6.101 ◽

2000 ◽

Vol 6 (3) ◽

pp. 101-109 ◽

Cited By ~ 11

Author(s):

Osamu Tsuruta ◽

Yuichiroh Tsuji ◽

Hiroshi Kawano ◽

Shiroh Miyazaki ◽

Masahide Watanabe ◽

...

Keyword(s):

Lymph Node ◽

Laparoscopic Surgery ◽

Lymph Node Metastasis ◽

Endoscopic Resection ◽

Lymphatic Invasion ◽

Colorectal Carcinomas ◽

Node Dissection ◽

Node Metastasis ◽

Level Of Invasion ◽

Histological Factors

We investigated the relationship between histological factors and lymph node metastasis in 77 lesions with submucosally invasive colorectal carcinomas to establish useful criteria for lesions in which endoscopic treatment alone results in cure of malignancy. There were positive correlations between histological factors, including the level of invasion, the histologic grade, presence or absence of lymphatic invasion, presence or absence of budding, and lymph node metastasis (p < 0.05, p < 0.05, p < 0.005, p < 0.01). The presence or absence of venous invasion did not influence lymph node metastasis. Laparoscopic surgery involving lymph node dissection should be indicated for sm1 carcinoma lesions with unfavorable histological factors. In lesions diagnosed as sm2 or sm3 prior to resection, intestinal resection involving lymph node dissection by laparoscopic surgery should be directly performed without endoscopic resection.In treating submucosally invasive colorectal carcinomas, the level of invasion can be clinically diagnosed, consequently endoscopic resection should be initially performed when lesions are evaluated as sm1 prior to resection. When histological investigation reveals sm1 carcinoma with histologic grade I (well-differentiated) or II (moderately-differentiated), and the absence of lymphatic invasion and budding, endoscopic treatment alone is sufficient.

Download Full-text