Acaricidal activity of Cymbopogon winterianus, Vitex negundo and Withania somnifera against synthetic pyrethroid resistant Rhipicephalus (Boophilus) microplus

ABSTRACT: This experiment was carried out to study the bioacaricidal activity of Amburana cearensis against engorged females of Rhipicephalus (Boophilus) microplus. The crude ethanolic extract from the leaves of A. cearensis was submitted to partition with organic solvents (hexane, chloroform and ethyl acetate) in order of increasing polarity; and evaluated using three treatments at concentrations of 5, 10 and 25mg ml-1. To evaluate the acaricidal activity, engorged females were submitted to the adult immersion test. Parameters analyzed were the weight of females; weight of egg mass; percentage of hatch; reproductive efficiency; expected rate of reproduction; and efficacy. For each extract and concentrating fraction, tests were performed in triplicate. Among all fractions, the hexane (2.5%) achieved the best results in all parameters analyzed: 52.7% of inhibition of oviposition; 39% of hatching rate; 3,271 index of reproductive efficiency; and 67.0% effectiveness. The extract obtained gradual dose response with increasing concentration; and could be used as an aid in the control of R. (B.) microplus therapy.

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Control of bovine tick [Rhipicephalus (Boophilus) microplus] with Brunfelsia uniflora leaf extract

Australian Journal of Crop Science ◽

10.21475/ajcs.19.13.06.p1653 ◽

2019 ◽

pp. 903-910

Author(s):

Elaine Yae Yamashita Sugauara ◽

ElisângelaYumi Sugauara ◽

Rosangela Rumi Sugauara ◽

Wanessa de Campos Bortolucci ◽

Herika Line Marko de Oliveira ◽

...

Keyword(s):

Ethyl Ester ◽

Leaf Extract ◽

Acid Ethyl Ester ◽

Acaricidal Activity ◽

Boophilus Microplus ◽

Ex Situ ◽

In Vitro Tests ◽

Free Living ◽

Crude Leaf Extract

Bovine tick has caused losses in livestock production profitability in Brazil. However, tick control has caused resistance of these ectoparasites against utilized acaricides. Alternative tick controls have been utilizing plants as sources of effective botanical acaricides. Brunfelsia uniflora is a Brazilian plant with antimicrobial and antioxidant activity; however, there are no reports on its acaricidal activity. Therefore, this study aimed to evaluate the chemical composition of B. uniflora leaf ethanolic extract and its efficiency to control bovine tick in vitro and free-living stage ex situ. The crude leaf extract was analyzed by gas chromatographer coupled to mass spectrometer (GC-MS) with identification of 17 compounds. The major compounds were phytol (22.96%), 9,12,15-octadecatrienoic acid, ethyl ester (Z,Z,Z) (21.18%), hexadecanoic acid, ethyl ester (12.74%) and vitamin E (8.77%). The crude extract presented acaricidal activity in vitro against ingurgitated adult females, larvae and eggs of bovine tick. The LC99.9 for larvae was 103.21 mg mL-1 in in vitro tests and was 100% efficient for ex situ larva test (free-living stage). B. uniflora leaf extract is an alternative for the control of the bovine tick cycle, mainly in the free-living stage (non-parasitic stage) under field conditions.

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In vitro evaluation of acaricidal activity of novel green silver nanoparticles against deltamethrin resistance Rhipicephalus (Boophilus) microplus

Veterinary Parasitology ◽

10.1016/j.vetpar.2017.02.017 ◽

2017 ◽

Vol 237 ◽

pp. 130-136 ◽

Cited By ~ 9

Author(s):

B. Avinash ◽

R. Venu ◽

M. Alpha Raj ◽

K. Srinivasa Rao ◽

Ch. Srilatha ◽

...

Keyword(s):

Silver Nanoparticles ◽

Acaricidal Activity ◽

Boophilus Microplus ◽

In Vitro Evaluation ◽

Deltamethrin Resistance

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Acaricidal activity of Lippia gracilis essential oil and its major constituents on the tick Rhipicephalus (Boophilus) microplus

Veterinary Parasitology ◽

10.1016/j.vetpar.2012.12.046 ◽

2013 ◽

Vol 195 (1-2) ◽

pp. 198-202 ◽

Cited By ~ 48

Author(s):

Elizangela Mércia de Oliveira Cruz ◽

Livio Martins Costa-Junior ◽

Jessika Andreza Oliveira Pinto ◽

Darlisson de Alexandria Santos ◽

Sandra Alves de Araujo ◽

...

Keyword(s):

Essential Oil ◽

Acaricidal Activity ◽

Boophilus Microplus

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Chemical Composition and Acaricidal Activity of Nemuaron vieillardii Essential Oil against the Cattle Tick Rhipicephalus (Boophilus) microplus

Natural Product Communications ◽

10.1177/1934578x1601101235 ◽

2016 ◽

Vol 11 (12) ◽

pp. 1934578X1601101

Author(s):

Nicolas Lebouvier ◽

Thomas Hue ◽

Joseph Brophy ◽

Edouard Hnawia ◽

Mohammed Nour

Keyword(s):

Gas Chromatography ◽

Chemical Composition ◽

Essential Oil ◽

Tick Control ◽

Acaricidal Activity ◽

Boophilus Microplus ◽

Gas Chromatography Mass Spectrometry ◽

Cattle Tick ◽

Caryophyllene Oxide ◽

Human Carcinogen

Essential oil from leaves of Nemuaron vieillardii (Baill.) Baill., a shrub used in the kanak pharmacopeia, was analyzed by gas chromatography and combined gas chromatography-mass spectrometry. The main compounds identified were safrole (49.7%), linalool (8.0%), δ-cadinene (5.1%), caryophyllene oxide (4%) and α-copaene (2.4%). This chemical composition is consistent with leaf oils of the Atherospermataceae family and demonstrates the proximity of the two genera Nemuaron and Atherosperma as regards to their essential oil compositions. The modified Larval Packet Test (LPT) was used to assess acaricidal effect of N. vieillardii essential oil on larvae of the cattle tick Rhipicephalus (Boophilus) microplus and LC50 was then calculated to 14.67%. High content of safrole in this oil which is suspected of being a human carcinogen, poses the problem of the use of this oil in a perspective of development of alternative tick control strategy and in the traditional medicinal consumption of Nemuaron vieillardii.

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