scholarly journals Control of bovine tick [Rhipicephalus (Boophilus) microplus] with Brunfelsia uniflora leaf extract

2019 ◽  
pp. 903-910
Author(s):  
Elaine Yae Yamashita Sugauara ◽  
ElisângelaYumi Sugauara ◽  
Rosangela Rumi Sugauara ◽  
Wanessa de Campos Bortolucci ◽  
Herika Line Marko de Oliveira ◽  
...  
Keyword(s):  
Ethyl Ester ◽  
Leaf Extract ◽  
Acid Ethyl Ester ◽  
Acaricidal Activity ◽  
Boophilus Microplus ◽  
Ex Situ ◽  
In Vitro Tests ◽  
Free Living ◽  
Crude Leaf Extract

Bovine tick has caused losses in livestock production profitability in Brazil. However, tick control has caused resistance of these ectoparasites against utilized acaricides. Alternative tick controls have been utilizing plants as sources of effective botanical acaricides. Brunfelsia uniflora is a Brazilian plant with antimicrobial and antioxidant activity; however, there are no reports on its acaricidal activity. Therefore, this study aimed to evaluate the chemical composition of B. uniflora leaf ethanolic extract and its efficiency to control bovine tick in vitro and free-living stage ex situ. The crude leaf extract was analyzed by gas chromatographer coupled to mass spectrometer (GC-MS) with identification of 17 compounds. The major compounds were phytol (22.96%), 9,12,15-octadecatrienoic acid, ethyl ester (Z,Z,Z) (21.18%), hexadecanoic acid, ethyl ester (12.74%) and vitamin E (8.77%). The crude extract presented acaricidal activity in vitro against ingurgitated adult females, larvae and eggs of bovine tick. The LC99.9 for larvae was 103.21 mg mL-1 in in vitro tests and was 100% efficient for ex situ larva test (free-living stage). B. uniflora leaf extract is an alternative for the control of the bovine tick cycle, mainly in the free-living stage (non-parasitic stage) under field conditions.

scholarly journals Acaricidal activity of Furcraea foetida leaf extract against engorged female Rhipicephalus (Boophilus) microplus ticks

2021 ◽  
Vol 37 ◽  
pp. e37031
Author(s):  
Henrique Aparecido de Sousa Martins ◽  
Maria de Fatima Pereira ◽  
Enéas Ricardo Konzen ◽  
Gilvano Ebling Brondani ◽  
Wellington Ferreira Campos
Keyword(s):  
Crude Extract ◽  
Leaf Extract ◽  
Skin Lesions ◽  
Acaricidal Activity ◽  
Boophilus Microplus ◽  
Economic Damage ◽  
Attractive Alternative ◽  
Dependent Manner ◽  
Dose Dependent

The Rhipicephalus (Boophilus) microplus tick is a major concern for the livestock market worldwide, as it causes serious economic damage. Plant-derived acaricides are an attractive alternative to control this ectoparasite and limit the development of resistance. Therefore, the aim of this study was to evaluate the acaricidal activity of Furcraea foetida leaf extract against engorged female R. (B.) microplus ticks. Our in vitro bioassays showed that the crude extract of leaves from F. foetida caused hemorrhagic swelling and skin lesions in the ticks, and three days of treatment caused 100% mortality. Dose-response assay indicated that this toxicity effect was dose-dependent. Similar effects were observed when the crude extract from F. foetida leaves was denatured by boiling at 100°C. These results suggest that the toxicity of the leaf extract might be associated with thermostable biomolecules. Together, our results show for the first time that the crude extract of F. foetida leaves has acaricidal activity against engorged female R. (B.) microplus ticks and it acts in a dose-dependent manner.

Sonochemical synthesis of 2-substituted nicotinic acid ethyl ester derivatives: their in vitro and in silico evaluation against SIRT1

2021 ◽  
pp. 131069
Author(s):  
Chandra Sekhar Challa ◽  
Naresh Kumar Katari ◽  
Varadacharyulu Nallanchakravarthula ◽  
Devanna Nayakanti ◽  
Ravikumar Kapavarapu ◽  
...  
Keyword(s):  
Nicotinic Acid ◽  
Ethyl Ester ◽  
In Silico ◽  
Acid Ethyl Ester ◽  
Sonochemical Synthesis

In vitro evaluation of acaricidal activity of novel green silver nanoparticles against deltamethrin resistance Rhipicephalus (Boophilus) microplus

2017 ◽  
Vol 237 ◽  
pp. 130-136 ◽  
Author(s):  
B. Avinash ◽  
R. Venu ◽  
M. Alpha Raj ◽  
K. Srinivasa Rao ◽  
Ch. Srilatha ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
Acaricidal Activity ◽  
Boophilus Microplus ◽  
In Vitro Evaluation ◽  
Deltamethrin Resistance

scholarly journals Baccharis dracunculifolia DC (Asteraceae) leaf and flower essential oils to control Rhipicephalus microplus Canestrini (Arachnida: Ixodidae) in the free-living stage

2020 ◽  
Vol 9 (10) ◽  
pp. e5049108788
Author(s):  
Luciane Neris Cazella ◽  
Herika Line de Marko de Oliveira ◽  
Wanessa de Campos Bortolucci ◽  
Isabelle Luiz Rahal ◽  
Irinéia Paulina Baretta ◽  
...  
Keyword(s):  
Essential Oil ◽  
Essential Oils ◽  
Biological Activities ◽  
Probit Analysis ◽  
Rhipicephalus Microplus ◽  
Acaricidal Activity ◽  
Egg Laying ◽  
Ex Situ ◽  
Egg Hatchability

Baccharis dracunculifolia, native to Brazil and the main source of “green propolis”, has been reported with several biological activities, and may be a source of bovine tick control substituting synthetic acaricides. Objective: to evaluate the in vitro and ex situ acaricidal activity of B. dracunculifolia leaf and flower essential oils against Rhipicephalus microplus. Methodology: the essential oils were extracted by hydrodistillation and analyzed by a gas chromatography coupled to mass spectrometry; the acaricidal activity of the essential oil was evaluated in vitro against adult females and against the egg hatchability; moreover, the acaricidal activity against tick larvae was evaluated in vitro and ex situ. Results: the major class of the essential oils was oxygenated sesquiterpene (55.1% leaves 50.4% flowers) and the main compounds were (21.5% leaves; 20.6% flowers) and spathulenol (21.8% leaves; 20.3% flowers). The essential oil at 500 mg/mL was effective to control egg hatchability with a reduction of egg laying capacity and decrease of number of adult ticks and larvae. The larvicidal activity of the essential oil had LC99.9 from 35 to 37 mg/mL by probit analysis, and the essential oil from 11 to 14 mg/mL presented 85 to 95% of treatment efficiency in the ex situ test. Conclusion: B. dracunculifolia leaf and flower essential oils are stable and have application potential to control bovine ticks.

scholarly journals In vitro efficacy of essential oils with different concentrations of 1,8-cineole against Rhipicephalus (Boophilus) microplus

2018 ◽  
Vol 27 (2) ◽  
pp. 203-210 ◽  
Author(s):  
Karina Neoob de Carvalho Castro ◽  
Kirley Marques Canuto ◽  
Edy de Sousa Brito ◽  
Lívio Martins Costa-Júnior ◽  
Ivanilza Moreira de Andrade ◽  
...  
Keyword(s):  
Gas Chromatography ◽  
Essential Oils ◽  
Probit Analysis ◽  
Acaricidal Activity ◽  
Boophilus Microplus ◽  
Gas Chromatography Mass Spectrometry ◽  
Flame Ionization Detection ◽  
Flame Ionization ◽  
Alpinia Zerumbet

Abstract The aim of this study was to evaluate the acaricidal activity of essential oils from three species of plants with intermediary concentrations of 1,8-cineole against the tick species Rhipicephalus (Boophilus) microplus. For this purpose, five serial concentrations (100.0, 50.0, 25.0, 12.5, 6.2 mg/mL) of essential oils from Mesosphaerum suaveolens (L.) Kuntze, Ocimum gratissimum L. and Alpinia zerumbet (Pers.) B. L. Burtt & R. M. Sm. were used on larval packet and adult immersion tests. The essential oils were analysed by gas chromatography-mass spectrometry (GC/MS) and gas chromatography-flame ionization detection (GC-FID), being detected 35.8, 24.7 and 24.0% of 1.8-cineol in the oils of M. suaveolens, O. gratissimum and A. zerumbet, respectively. The lethal concentration (LC 50) of each oil for larvae and engorged females was calculated through Probit analysis. All essential oils showed high efficacy (≥ 95.0%) on engorged females at the 100.0 mg/mL concentration. In regards to larvae, O. gratissimum (LC 50 = 11.9 mg/mL) was the most potent, followed by the A. zerumbet (LC50 = 19.7 mg/mL) and the M. suaveolens (LC50 = 51.6 mg/mL) essential oils. These results show that other compounds interfere with 1,8-cineole action.

scholarly journals In vitro Antifungal Activity of Senna alata Linn. Crude Leaf Extract

2010 ◽  
Vol 5 (3) ◽  
pp. 275-284 ◽  
Author(s):  
W.F. Sule ◽  
I.O. Okonko ◽  
T.A. Joseph ◽  
M.O. Ojezele ◽  
J.C. Nwanze ◽  
...  
Keyword(s):  
Antifungal Activity ◽  
Leaf Extract ◽  
Crude Leaf Extract ◽  
Senna Alata ◽  
In Vitro Antifungal Activity

scholarly journals Efficiency of tick biotherapic on the control of infestation by Rhipicephalus (Boophilus) microplus in Dutch dairy cows.

2021 ◽  
Vol 9 (33) ◽  
pp. 156-164
Author(s):  
Zilda Cristiani Gazim ◽  
Fabiana Borges Padilha Ferreira ◽  
Aristeu Vieira Da Silva ◽  
Kelly Cristina Bolognese ◽  
Ewerton Merlin ◽  
...  
Keyword(s):  
Boophilus Microplus ◽  
Mineral Salt ◽  
Cattle Tick ◽  
Reproductive Efficiency ◽  
In Vitro Tests ◽  
Egg Mass ◽  
Hatching Rate ◽  
Egg Hatching ◽  
Significant Difference

Background: cattle tick Rhipicephalus (Boophilus) microplus poses serious problems for farmers in Brazil, especially because the parasite easily develops resistance to pesticide agents. For this reason, together with other factors including environmental, human and animal contamination and costs, alternative approaches have been sought for. Aims: this study sough to evaluate the efficiency of a tick biotherapic on tick-infested cows. Methods: 34 dairy Dutch cows were divided in 2 groups: one group received 100g/day of mineral salt supplement impregnated with tick biotherapic 12cH for 6 months, and then in alternate days with tick biotherapic 30cH to complete 28 months of treatment; the other group (control) received only the mineral salt supplement. After 28 months of treatment, engorged Rhipicephalus (boophilus) microplus females were collected in both groups, counted and weighed; in vitro tests were carried out to assess mass of ticks; egg mass; egg-hatching rate; and reproductive efficiency. Results: There was significant difference between both groups for all parameters evaluated; tick-mass (p = 0.0008); egg mass (p=0.0044); egg-hatching rate (p= 0.0017); and reproductive efficiency (p = 0.0044). Conclusion: treatment with tick biotherapic significantly decreased the mass of engorged females, deposition and hatching rate of eggs, resulting consequently in the decrease of the reproductive efficiency of ticks.

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