In vitro evaluation of acaricidal activity of novel green silver nanoparticles against deltamethrin resistance Rhipicephalus (Boophilus) microplus

Bovine tick has caused losses in livestock production profitability in Brazil. However, tick control has caused resistance of these ectoparasites against utilized acaricides. Alternative tick controls have been utilizing plants as sources of effective botanical acaricides. Brunfelsia uniflora is a Brazilian plant with antimicrobial and antioxidant activity; however, there are no reports on its acaricidal activity. Therefore, this study aimed to evaluate the chemical composition of B. uniflora leaf ethanolic extract and its efficiency to control bovine tick in vitro and free-living stage ex situ. The crude leaf extract was analyzed by gas chromatographer coupled to mass spectrometer (GC-MS) with identification of 17 compounds. The major compounds were phytol (22.96%), 9,12,15-octadecatrienoic acid, ethyl ester (Z,Z,Z) (21.18%), hexadecanoic acid, ethyl ester (12.74%) and vitamin E (8.77%). The crude extract presented acaricidal activity in vitro against ingurgitated adult females, larvae and eggs of bovine tick. The LC99.9 for larvae was 103.21 mg mL-1 in in vitro tests and was 100% efficient for ex situ larva test (free-living stage). B. uniflora leaf extract is an alternative for the control of the bovine tick cycle, mainly in the free-living stage (non-parasitic stage) under field conditions.

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In-vitro Anthelmintic and Acaricidal Activity of Nicotiana tabacum Leaf Extract Mediated AgNPs Against Rhipicephalus (Boophilus) microplus

International Journal of Pure & Applied Bioscience ◽

10.18782/2320-7051.5298 ◽

2017 ◽

Vol 5 (5) ◽

pp. 1013-1022 ◽

Cited By ~ 1

Author(s):

B. Avinash ◽

Keyword(s):

Nicotiana Tabacum ◽

Leaf Extract ◽

Acaricidal Activity ◽

Boophilus Microplus

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In vitro efficacy of essential oils with different concentrations of 1,8-cineole against Rhipicephalus (Boophilus) microplus

Revista Brasileira de Parasitologia Veterinária ◽

10.1590/s1984-296120180015 ◽

2018 ◽

Vol 27 (2) ◽

pp. 203-210 ◽

Cited By ~ 9

Author(s):

Karina Neoob de Carvalho Castro ◽

Kirley Marques Canuto ◽

Edy de Sousa Brito ◽

Lívio Martins Costa-Júnior ◽

Ivanilza Moreira de Andrade ◽

...

Keyword(s):

Gas Chromatography ◽

Essential Oils ◽

Probit Analysis ◽

Acaricidal Activity ◽

Boophilus Microplus ◽

Gas Chromatography Mass Spectrometry ◽

Flame Ionization Detection ◽

Flame Ionization ◽

Alpinia Zerumbet

Abstract The aim of this study was to evaluate the acaricidal activity of essential oils from three species of plants with intermediary concentrations of 1,8-cineole against the tick species Rhipicephalus (Boophilus) microplus. For this purpose, five serial concentrations (100.0, 50.0, 25.0, 12.5, 6.2 mg/mL) of essential oils from Mesosphaerum suaveolens (L.) Kuntze, Ocimum gratissimum L. and Alpinia zerumbet (Pers.) B. L. Burtt & R. M. Sm. were used on larval packet and adult immersion tests. The essential oils were analysed by gas chromatography-mass spectrometry (GC/MS) and gas chromatography-flame ionization detection (GC-FID), being detected 35.8, 24.7 and 24.0% of 1.8-cineol in the oils of M. suaveolens, O. gratissimum and A. zerumbet, respectively. The lethal concentration (LC 50) of each oil for larvae and engorged females was calculated through Probit analysis. All essential oils showed high efficacy (≥ 95.0%) on engorged females at the 100.0 mg/mL concentration. In regards to larvae, O. gratissimum (LC 50 = 11.9 mg/mL) was the most potent, followed by the A. zerumbet (LC50 = 19.7 mg/mL) and the M. suaveolens (LC50 = 51.6 mg/mL) essential oils. These results show that other compounds interfere with 1,8-cineole action.

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Avaliação in vitro de diferentes formulações acaricidas sobre o parâmetro reprodutivos de fêmeas ingurgitadas de rhipicephalus (Boophilus) Microplus / In vitro evaluation of different formations acaricides on the reproductive parameter of ingurgitated females of rhipicephalus (Boophilus) Microplus

Brazilian Journal of Development ◽

10.34117/bjdv7n9-108 ◽

2021 ◽

Vol 7 (9) ◽

pp. 87922-87935

Author(s):

Milena C. Bogo ◽

Cristine De S. Alves ◽

Matheus H. D. Silva ◽

Mateus P. Do Nascimento ◽

Valdomiro Pereira ◽

...

Keyword(s):

Boophilus Microplus ◽

Reproductive Parameter ◽

In Vitro Evaluation

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Acaricidal activity of Furcraea foetida leaf extract against engorged female Rhipicephalus (Boophilus) microplus ticks

Bioscience Journal ◽

10.14393/bj-v37n0a2021-48254 ◽

2021 ◽

Vol 37 ◽

pp. e37031

Author(s):

Henrique Aparecido de Sousa Martins ◽

Maria de Fatima Pereira ◽

Enéas Ricardo Konzen ◽

Gilvano Ebling Brondani ◽

Wellington Ferreira Campos

Keyword(s):

Crude Extract ◽

Leaf Extract ◽

Skin Lesions ◽

Acaricidal Activity ◽

Boophilus Microplus ◽

Economic Damage ◽

Attractive Alternative ◽

Dependent Manner ◽

Dose Dependent

The Rhipicephalus (Boophilus) microplus tick is a major concern for the livestock market worldwide, as it causes serious economic damage. Plant-derived acaricides are an attractive alternative to control this ectoparasite and limit the development of resistance. Therefore, the aim of this study was to evaluate the acaricidal activity of Furcraea foetida leaf extract against engorged female R. (B.) microplus ticks. Our in vitro bioassays showed that the crude extract of leaves from F. foetida caused hemorrhagic swelling and skin lesions in the ticks, and three days of treatment caused 100% mortality. Dose-response assay indicated that this toxicity effect was dose-dependent. Similar effects were observed when the crude extract from F. foetida leaves was denatured by boiling at 100°C. These results suggest that the toxicity of the leaf extract might be associated with thermostable biomolecules. Together, our results show for the first time that the crude extract of F. foetida leaves has acaricidal activity against engorged female R. (B.) microplus ticks and it acts in a dose-dependent manner.

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