Transcriptome analysis and identification of significantly differentially expressed genes in Holstein calves subjected to severe thermal stress

2017 ◽  
Vol 61 (11) ◽  
pp. 1993-2008 ◽  
Author(s):  
Krishnamoorthy Srikanth ◽  
Eunjin Lee ◽  
Anam Kwan ◽  
Youngjo Lim ◽  
Junyep Lee ◽  
...  
Keyword(s):  
Thermal Stress ◽  
Differentially Expressed Genes ◽  
Transcriptome Analysis ◽  
Differentially Expressed ◽  
Holstein Calves

scholarly journals Transcriptome analysis identifies differentially expressed genes in the progenies of a cross between two low phytic acid soybean mutants

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Hangxia Jin ◽  
Xiaomin Yu ◽  
Qinghua Yang ◽  
Xujun Fu ◽  
Fengjie Yuan
Keyword(s):  
Developmental Stage ◽  
Phytic Acid ◽  
Differentially Expressed Genes ◽  
Transcriptome Analysis ◽  
Defense Mechanisms ◽  
Developmental Stages ◽  
Sucrose Metabolism ◽  
Differentially Expressed ◽  
Sequencing Analysis ◽  
Seed Developmental Stage

AbstractPhytic acid (PA) is a major antinutrient that cannot be digested by monogastric animals, but it can decrease the bioavailability of micronutrients (e.g., Zn and Fe). Lowering the PA content of crop seeds will lead to enhanced nutritional traits. Low-PA mutant crop lines carrying more than one mutated gene (lpa) have lower PA contents than mutants with a single lpa mutant gene. However, little is known about the link between PA pathway intermediates and downstream regulatory activities following the mutation of these genes in soybean. Consequently, we performed a comparative transcriptome analysis using an advanced generation recombinant inbred line with low PA levels [2mlpa (mips1/ipk1)] and a sibling line with homozygous non-mutant alleles and normal PA contents [2MWT (MIPS1/IPK1)]. An RNA sequencing analysis of five seed developmental stages revealed 7945 differentially expressed genes (DEGs) between the 2mlpa and 2MWT seeds. Moreover, 3316 DEGs were associated with 128 metabolic and signal transduction pathways and 4980 DEGs were annotated with 345 Gene Ontology terms related to biological processes. Genes associated with PA metabolism, photosynthesis, starch and sucrose metabolism, and defense mechanisms were among the DEGs in 2mlpa. Of these genes, 36 contributed to PA metabolism, including 22 genes possibly mediating the low-PA phenotype of 2mlpa. The expression of most of the genes associated with photosynthesis (81 of 117) was down-regulated in 2mlpa at the late seed developmental stage. In contrast, the expression of three genes involved in sucrose metabolism was up-regulated at the late seed developmental stage, which might explain the high sucrose content of 2mlpa soybeans. Furthermore, 604 genes related to defense mechanisms were differentially expressed between 2mlpa and 2MWT. In this study, we detected a low PA content as well as changes to multiple metabolites in the 2mlpa mutant. These results may help elucidate the regulation of metabolic events in 2mlpa. Many genes involved in PA metabolism may contribute to the substantial decrease in the PA content and the moderate accumulation of InsP3–InsP5 in the 2mlpa mutant. The other regulated genes related to photosynthesis, starch and sucrose metabolism, and defense mechanisms may provide additional insights into the nutritional and agronomic performance of 2mlpa seeds.

Transcriptome analysis reveals differentially expressed genes associated with the mantled homeotic flowering abnormality in oil palm (Elaeis guineensis)

2010 ◽  
Vol 7 (1) ◽  
pp. 169-182 ◽  
Author(s):  
Thierry Beulé ◽  
Céline Camps ◽  
Ségolène Debiesse ◽  
Christine Tranchant ◽  
Stéphane Dussert ◽  
...  
Keyword(s):  
Differentially Expressed Genes ◽  
Transcriptome Analysis ◽  
Oil Palm ◽  
Elaeis Guineensis ◽  
Differentially Expressed

Gonadal transcriptome analysis of the common carp, Cyprinus carpio: Identification of differentially expressed genes and SSRs

2019 ◽  
Vol 279 ◽  
pp. 67-77 ◽  
Author(s):  
Arumugam Anitha ◽  
Yugantak-Raj Gupta ◽  
Seetharam Deepa ◽  
Manjappa Ningappa ◽  
Karani Boraiah Rajanna ◽  
...  
Keyword(s):  
Differentially Expressed Genes ◽  
Common Carp ◽  
Cyprinus Carpio ◽  
Transcriptome Analysis ◽  
Differentially Expressed ◽  
Common Carp Cyprinus Carpio ◽  
Carp Cyprinus Carpio ◽  
The Common

scholarly journals Transcriptome analysis of differentially expressed genes and pathways associated with mitoxantrone treatment prostate cancer

2018 ◽  
Vol 23 (3) ◽  
pp. 1987-2000 ◽  
Author(s):  
Sanqiang Li ◽  
Ruifang Li ◽  
Yu Ma ◽  
Cong Zhang ◽  
Tao Huang ◽  
...  
Keyword(s):  
Prostate Cancer ◽  
Differentially Expressed Genes ◽  
Transcriptome Analysis ◽  
Differentially Expressed
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