Persistence and detection of black truffle ectomycorrhizas in plantations: comparison between two field detection methods

Mycorrhiza ◽  
2014 ◽  
Vol 24 (S1) ◽  
pp. 39-46 ◽  
Author(s):  
Sergio Sánchez ◽  
Teresa Ágreda ◽  
Beatriz Águeda ◽  
María Martín ◽  
Ana María de Miguel ◽  
...  
Keyword(s):  
Detection Methods ◽  
Black Truffle ◽  
Field Detection

scholarly journals Ultrasensitive and Whole-Course Encapsulated Field Detection of 2019-nCoV Gene Applying Exponential Amplification from RNA Combined with Chemical Probes

2020 ◽  
Author(s):  
Simin Xia ◽  
Xi Chen
Keyword(s):  
Reverse Transcription ◽  
Rna Virus ◽  
Detection Methods ◽  
Chemical Probes ◽  
Trace Level ◽  
Field Detection ◽  
Global Epidemic ◽  
Affinity Probe ◽  
Highly Sensitive ◽  
Human Infections

The newly identified 2019-nCoV (SARS-CoV-2) RNA virus has caused over 80,000 laboratory-confirmed human infections in China (as of Mar. 1st, 2020), and is now becoming a global epidemic issue. As a result, highly sensitive, reliable and field-deployable methods to detect 2019-nCoV that can be developed in a shortest possible time are urgently needed. In this work, we introduce whole-course encapsulated and ultrasensitive field detection methods against 2019-nCoV gene. We applied isothermal exponential amplification methods via reverse transcription plus subsequent enzymatic recombinase amplification (ERA), a modified recombinase polymerase amplification (RPA), to amplify trace level of 2019-nCoV RNA. We designed both exo FRET probe and nfo affinity probe for easy detection of the amplified nucleic acids. These methods can be developed within a few days and as low as 0.32 aM (i.e. 0.32×10-18 M) of RNA can be reliably detected.<b></b><br>

scholarly journals Ultrasensitive and Whole-Course Encapsulated Field Detection of 2019-nCoV Gene Applying Exponential Amplification from RNA Combined with Chemical Probes

2020 ◽  
Author(s):  
Simin Xia ◽  
Xi Chen
Keyword(s):  
Reverse Transcription ◽  
Rna Virus ◽  
Detection Methods ◽  
Chemical Probes ◽  
Trace Level ◽  
Field Detection ◽  
Global Epidemic ◽  
Affinity Probe ◽  
Highly Sensitive ◽  
Human Infections

The newly identified 2019-nCoV (SARS-CoV-2) RNA virus has caused over 80,000 laboratory-confirmed human infections in China (as of Mar. 1st, 2020), and is now becoming a global epidemic issue. As a result, highly sensitive, reliable and field-deployable methods to detect 2019-nCoV that can be developed in a shortest possible time are urgently needed. In this work, we introduce whole-course encapsulated and ultrasensitive field detection methods against 2019-nCoV gene. We applied isothermal exponential amplification methods via reverse transcription plus subsequent enzymatic recombinase amplification (ERA), a modified recombinase polymerase amplification (RPA), to amplify trace level of 2019-nCoV RNA. We designed both exo FRET probe and nfo affinity probe for easy detection of the amplified nucleic acids. These methods can be developed within a few days and as low as 0.32 aM (i.e. 0.32×10-18 M) of RNA can be reliably detected.<b></b><br>

scholarly journals Field detection of material defect of dry transformer winding

2020 ◽  
Vol 165 ◽  
pp. 05023
Author(s):  
Chao Feng ◽  
Jiarui Hu ◽  
Hongchun Sun ◽  
Jun Liu ◽  
Siyuan Yang ◽  
...  
Keyword(s):  
Density Variation ◽  
Residential Area ◽  
Detection Methods ◽  
Preliminary Treatment ◽  
Field Detection ◽  
Transformer Winding ◽  
The Law ◽  
Variation Rule ◽  
Matter Detection

To detect the material defect of dry transformer winding, a series of study were carried out concerning about the law of absorption ray intensity of matter, detection methods and procedures and Photographic density variation rule of copper and aluminium. The field detection was also conducted in residential area without preliminary treatment.

Relativity Analysis on the Detection Methods of Compaction Degree for Expressway Subgrade

2011 ◽  
Vol 413 ◽  
pp. 252-257
Author(s):  
Gui Juan Zhao
Keyword(s):  
Standard Method ◽  
Test Section ◽  
Detection Methods ◽  
Nuclear Density ◽  
Drop Hammer ◽  
Field Detection ◽  
Ring Method ◽  
The Relationship ◽  
Compaction Degree

A test section was selected in Luo-San expressway.Four detection methods of compaction degree which are sand cone method, cutting ring, nuclear density instrument and drop hammer frequent mensuration£¨LY-1 apparatus£©have been adopted in testing road.And the detection results about four methods were analyzed and compared.Sand cone method was conducted as a standard method of field detection for compaction degree, other three methods were regressed with sand cone method,and the relationship was obtained. The relevance of cutting ring method with sand cone method is excellent,the relevance of other two methods with sand cone method is slightly bad which may because of the soil and construction methods.But in general, it is approved that sand cone method method which act as the standard one of detection for compaction degree is worthwhile extending.

DNA nick end labeling: a reliable marker for apoptosis?

1995 ◽  
Vol 53 ◽  
pp. 962-963
Author(s):  
Anne F. Bushnell ◽  
Sarah Webster ◽  
Lynn S. Perlmutter
Keyword(s):  
Cell Death ◽  
Cultured Cells ◽  
Apoptotic Cell ◽  
Morphological Characteristics ◽  
Detection Methods ◽  
Tissue Preparation ◽  
Preparation Methods ◽  
Dna Laddering ◽  
Disease States ◽  
Reliable Marker

Apoptosis, or programmed cell death, is an important mechanism in development and in diverse disease states. The morphological characteristics of apoptosis were first identified using the electron microscope. Since then, DNA laddering on agarose gels was found to correlate well with apoptotic cell death in cultured cells of dissimilar origins. Recently numerous DNA nick end labeling methods have been developed in an attempt to visualize, at the light microscopic level, the apoptotic cells responsible for DNA laddering.The present studies were designed to compare various tissue processing techniques and staining methods to assess the occurrence of apoptosis in post mortem tissue from Alzheimer's diseased (AD) and control human brains by DNA nick end labeling methods. Three tissue preparation methods and two commercial DNA nick end labeling kits were evaluated: the Apoptag kit from Oncor and the Biotin-21 dUTP 3' end labeling kit from Clontech. The detection methods of the two kits differed in that the Oncor kit used digoxigenin dUTP and anti-digoxigenin-peroxidase and the Clontech used biotinylated dUTP and avidinperoxidase. Both used 3-3' diaminobenzidine (DAB) for final color development.

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