scholarly journals Haematological changes in Wistar rats experimentally infected with Trypanosoma congolense and Trypanosoma brucei brucei obtained from North-west Nigeria

2021 ◽  
Vol 19 (2) ◽  
pp. 73-80
Author(s):  
A.O. Fajinmi ◽  
O.O. Faleke ◽  
A.A. Magaji ◽  
U.M. Chafe ◽  
M.A. Kassim ◽  
...  
Keyword(s):  
Blood Cell ◽  
Trypanosoma Brucei ◽  
Wistar Rats ◽  
Trypanosoma Congolense ◽  
Control Group ◽  
Blood Cell Count ◽  
Trypanosoma Brucei Brucei ◽  
Group A ◽  
Haematological Changes ◽  
Group D

This study determined haematological changes in Wistar rats experimentally infected with local strains of Trypanosoma congolense and Trypanosoma brucei brucei. Forty-five Wistar rats between 10 – 12 weeks old weighing between 210 – 240 g were used. The Wistar rats were randomly divided into four groups (A, B, C and D), with the infected groups (B, C and D) having 10 rats each, while the uninfected control group (A) had 15 rats. Group A rats were not infected and served as the control, group B were infected with Trypanosoma congolense, group C were infected with Trypanosoma brucei brucei and group D were co-infected with Trypanosoma congolense and Trypanosoma brucei brucei. Infection was achieved using 0.1mL of blood containing approximately 1 × 103 trypanosomes intraperitoneally into each Wistar rat in the infected groups. Clinical signs were observed. The changes in the blood cells were assayed in the groups post-infection. Duncan’s Least Square Deviation showed significantly (p<0.05) higher parasitaemia in infected groups. However, group D showed a higher significant (p<0.05) difference in parasitaemia when compared to groups B and C. The pattern of mean parasitaemia for the infected groups, revealed a positive correlation with days of post-infection (p<0.05) before the decline. The packed cell volume, total red blood cell count and haemoglobin concentration were significantly (p<0.05) lower in infected groups B, C and D. The total white blood cell count, platelet counts and differential leucocyte count were significantly (p<0.05) lower in infected groups when compared to the uninfected group. These findings suggest that co-infection with Trypanosoma congolense and Trypanosoma brucei brucei obtained from Wurno and Ngaski in Sokoto and Kebbi States respectively produced a more damaging effect on haematological parameters.

scholarly journals Effects of Aqueous Extract of Basella alba L. leaves on Blood Cell Count in Rats

2019 ◽  
Vol 22 (1) ◽  
pp. 73-78
Author(s):  
Farhana Sabrin ◽  
AF Mohammed Shafiqul Alam ◽  
Muhammad Rashedul Islam ◽  
Md Elias Al Mamun ◽  
Jakir Ahmed Chowdhury
Keyword(s):  
Blood Cell ◽  
Cell Count ◽  
Aqueous Extract ◽  
Control Group ◽  
P Value ◽  
Blood Cell Count ◽  
Basella Alba ◽  
Group A ◽  
Group B ◽  
Group D

The effect of aqueous extract of Basella alba (puishak) leaves on blood cell count of rats was studied. Forty rats of both sexes weighing between 100-150 gm were used. The rats were divided into four groups (7 rats in each group), with group A as the control group and experimental groups were denoted as B, C and D. With all aseptic precautions, aqueous extract of Basella alba (Puishak) leaves was administered into the three different doses (For group B: 60 mg/kg bw, group C: 80 mg/kg bw and group D: 100 mg/kg bw). Control group A also received distilled water as a placebo at the dose of 10 mg/kg bw for 14 days. At day 15, blood samples were collected and sent for haematological analysis. Data analysis of blood count profile of 28 rats revealed that there is an increased number of RBC, WBC and platelet count in experimental groups than in control group. ANOVA test revealed that increased blood cell counts following administration of aqueous leaves extract of keeves of B. alba were statistically significant (p value for each case was .0001<.05) than control group. Paired samples t test was performed to compare between baseline hematological parameters and parameters after 14 days of intervention. Then comparison between Group A & Group B, between Group A & Group C and between Group A & Group D were done. In all cases, probability (p) value < 0.05 was considered as statistically significant.This it is clearly evident that aqueous extract of B.alba has positive stimulant effect on blood cells count of rats. Moreover, it was found that increment of doses also increases the cell count that is positively correlated with the hypothesis. Bangladesh Pharmaceutical Journal 22(1): 73-78, 2019

scholarly journals Effects of aqueous leaf extract of Corchorus olitorius on the testis and blood testosterone level of adult Wistar rats

2021 ◽  
Vol 10 (2) ◽  
pp. 024-030
Author(s):  
Godwin Chinedu Uloneme ◽  
Demian Nnabuihe Ezejindu ◽  
Darlington Cyprian Akukwu ◽  
Amadi Chibundu Chiekezie
Keyword(s):  
Body Weight ◽  
Wistar Rats ◽  
Day Treatment ◽  
Control Group ◽  
Corchorus Olitorius ◽  
Group A ◽  
Normal Rat ◽  
Group B ◽  
Calcium Iron ◽  
Group D

Background: The extract of Corchorus olitorius has a reasonable content of vitamins A and C, calcium, iron and fibre, and therefore enjoys a universal application in the treatment of some disease conditions, even as the whole leaf is a very important component of food in so many cultures. Purpose: The study was designed to investigate the effect of Corchorus olitorius extracts on the testis of adult Wistar rats. Method: A total number of thirty two adult Wistar rats weighing between 180 and 200 grammes separated into four groups labeled A,B,C and D respectively were used for the study. Animals in group A which served as the control group were fed with the normal rat chow and water only. The group B rats were administered 100mg/kg body weight of aqueous extract of Corchorus olitorus; while those in group C were administered 500mg/kg body weight of the extract. The group D rats received 1000mg/kg body weight of the extract. For a period of four weeks, the different experimental animal groups received the respective aforementioned treatments once daily, around nine- o’clock in the morning through oral intubation. At the end of the 28 day treatment, the animals were sacrificed and the testes harvested for histological, investigation, and through cardiac puncture, blood samples for some hormonal studies was also collected and investigated using standard laboratory standards. Results: Observations made showed that the extract produced no histological distortions, degenerative or defective effects on the testicular tissues. The testosterone levels of group B, C, and D rats were observed to be significantly higher (P<0.005) than that of the group A (control group).ound: The extract of Corchorus olitorius has a reasonable content of vitamins A and C, calcium, iron and fibre, and therefore enjoys a universal application in the treatment of some disease conditions, even as the whole leaf is a very important component of food in so many cultures. Purpose: The study was designed to investigate the effect of Corchorus olitorius extracts on the testis of adult Wistar rats. Method: A total number of thirty two adult Wistar rats weighing between 180 and 200 grammes separated into four groups labeled A,B,C and D respectively were used for the study. Animals in group A which served as the control group were fed with the normal rat chow and water only. The group B rats were administered 100mg/kg body weight of aqueous extract of Corchorus olitorus; while those in group C were administered 500mg/kg body weight of the extract. The group D rats received 1000mg/kg body weight of the extract. For a period of four weeks, the different experimental animal groups received the respective aforementioned treatments once daily, around nine- o’clock in the morning through oral intubation. At the end of the 28 day treatment, the animals were sacrificed and the testes harvested for histological, investigation, and through cardiac puncture, blood samples for some hormonal studies was also collected and investigated using standard laboratory standards. Results: Observations made showed that the extract produced no histological distortions, degenerative or defective effects on the testicular tissues. The testosterone levels of group B, C, and D rats were observed to be significantly higher (P<0.005) than that of the group A (control group).

scholarly journals Effect of intranasal recombinant Mannheimia haemolytica vaccination on some haematological indices of goats infected with peste des petits ruminants virus

1970 ◽  
Vol 13 (1) ◽  
pp. 48-52
Author(s):  
OZ Tenuche ◽  
BO Emikpe
Keyword(s):  
Lymphocyte Count ◽  
Mannheimia Haemolytica ◽  
Control Group ◽  
Peste Des Petits Ruminants ◽  
Post Vaccination ◽  
Group A ◽  
Haematological Changes ◽  
Ppr Virus ◽  
Group B ◽  
Group D

There is dearth of information on the haematological changes associated with Mannheimia haemolytica vaccination in goats, hence this report which describes some haematological changes observed following vaccination with intranasal Recombinant Mannheimia haemolytica vaccine in goats naturally infected with peste des petits ruminants (PPR) virus. Twenty one (male, n=11; and female, n=10) goats were assigned to three vaccinated groups (A, B and D) with five goats per group (male: 3, female: 2), while the control group had 6 goats. Group A was vaccinated once intranasally, group B was vaccinated intranasally twice at one week interval and group D was vaccinated intranasally twice at two weeks interval . The control group (C) was not vaccinated. The vaccinated and control groups were challenged by comingling with pneumonic goats to simulate the field experience. PPR virus infection was later diagnosed in all the groups post vaccination. An average of four animals per treatment group in post-vaccination days were bled once weekly for six weeks (every week) to evaluate some haematological changes. The PCV values were within the normal range, while there was a decline in lymphocyte count at week 5, and a steady increase in neutrophil count in group A. In Group B, there was similar decline in lymphocyte count from the sixth week, while in groups C (Control) and D, the lymphocyte count declined at the 7th week, as the neutrophil counts increased. There were no significant changes in monocyte and eosinophil counts. The degree of changes in lymphocyte and neutrophil counts was mild in group B and marked in group D. This study revealed that intranasal vaccination of recombinant Mannheimia  haemolytica vaccine in the presence of PPR virus outbreak results in mild hematological derangement when the goats were vaccinated with  Mannheimia haemolytica bacterin at a week interval.Keywords: Goats, Haematology, Intranasal Recombinant Mannheimia haemolytica, Peste des Petits Ruminants, Vaccination

scholarly journals Subphenotypes in Patients with Septic Shock Receiving Vitamin C, Hydrocortisone, and Thiamine: A Retrospective Cohort Analysis

Nutrients ◽  
2019 ◽  
Vol 11 (12) ◽  
pp. 2976 ◽  
Author(s):  
Won-Young Kim ◽  
Jae-Woo Jung ◽  
Jae Chol Choi ◽  
Jong Wook Shin ◽  
Jae Yeol Kim
Keyword(s):  
Intensive Care Unit ◽  
Septic Shock ◽  
Intensive Care ◽  
Vitamin C ◽  
Blood Cell ◽  
White Blood Cell ◽  
Blood Cell Count ◽  
Group A ◽  
Group B ◽  
Group D

This study aimed to identify septic phenotypes in patients receiving vitamin C, hydrocortisone, and thiamine using temperature and white blood cell count. Data were obtained from septic shock patients who were also treated using a vitamin C protocol in a medical intensive care unit. Patients were divided into groups according to the temperature measurements as well as white blood cell counts within 24 h before starting the vitamin C protocol. In the study, 127 patients included who met the inclusion criteria. In the cohort, four groups were identified: “Temperature ≥37.1 °C, white blood cell count ≥15.0 1000/mm3” (group A; n = 27), “≥37.1 °C, <15.0 1000/mm3” (group B; n = 30), “<37.1 °C, ≥15.0 1000/mm3” (group C; n = 35) and “<37.1 °C, <15.0 1000/mm3” (group D; n = 35). The intensive care unit mortality rates were 15% for group A, 33% for group B, 34% for group C, and 49% for group D (p = 0.051). The temporal improvement in organ dysfunction and vasopressor dose seemed more apparent in group A patients. Our results suggest that different subphenotypes exist among sepsis patients treated using a vitamin C protocol, and clinical outcomes might be better for patients with the hyperinflammatory subphenotype.

scholarly journals Time-Dependent Testicular and Epididymal Damage in Rabbit Bucks Experimentally Infected with Trypanosoma brucei brucei

2021 ◽  
Vol 0 (0) ◽  
Author(s):  
Adewale Ayodeji Adeyeye ◽  
Musbaudeen Olayinka Alayande ◽  
Monsurat Bansima Adio ◽  
Lauratu Lawal Haliru ◽  
Aliyu Abdulrafiu
Keyword(s):  
Trypanosoma Brucei ◽  
Clinical Signs ◽  
Control Group ◽  
Pathological Changes ◽  
Epididymal Sperm ◽  
Trypanosoma Brucei Brucei ◽  
Group A ◽  
Time Of Infection ◽  
Gross Lesions ◽  
Group B

Abstract This study was designed to determine the progressive pathological changes in the testis and epididymis of rabbit bucks experimentally infected with Trypanosoma brucei brucei (T. brucei). Fifteen local rabbit bucks were divided into 2 groups comprising 8 bucks in group A and 7 bucks in group B. Bucks in group A were infected intraperitoneally with approximately 1.0x106 of T. brucei while bucks in group B served as the uninfected control. The bucks were closely monitored for clinical signs of trypanosomosis from the time of infection till the end of the study. One buck from each group was humanely sacrificed on days 9, 19, 29 and 38 post-infection (pi) and its testis and epididymis were examined for pathological changes. At post mortem, no gross lesions were observed in the infected bucks except for adhesion of the testis and epididymis to the scrotum on days 29 and 38 pi. There were no histological changes in the testis and epididymis of the infected buck sacrificed on day 9 pi apart from mild depletion of the epididymal sperm reserve. The buck infected on day 19 pi had moderate testicular degeneration but the epididymis was devoid of sperm reserve. Severe testicular and epididymal degeneration was observed in the buck on days 29 and 38 pi. The testis and epididymis of bucks from the control group were normal with evidence of spermatogenic activities and full epididymal sperm reserve throughout. The study shows that infected bucks were unfit for breeding from day 19 pi.

scholarly journals EFEK PEMBERIAN EKSTRAK KAYU MANIS (CINNAMOMUM BURMANNII) TERHADAP GAMBARAN HISTOPATOLOGI LAMBUNG TIKUS WISTAR YANG DIBERI ASPIRIN

2014 ◽  
Vol 2 (2) ◽  
Author(s):  
Janet Walangitan ◽  
Lily Loho ◽  
Meilany Durry
Keyword(s):  
Gastric Mucosa ◽  
Wistar Rats ◽  
Inflammatory Cells ◽  
Control Group ◽  
Therapeutic Effects ◽  
Cinnamon Extract ◽  
Group A ◽  
Cinnamomum Burmannii ◽  
Group B ◽  
Group D

Abstract: Cinnamon (Cinnamomum burmannii) is a traditional herbal plants which are often found in our daily life and has many benefits especially in health. This study were designed to know the effect of cinnamon on gastric mucosa given aspirin. This was an experimental research and used Wistar rats as the subject research.  The Wistar rats were randomly divided into 6 mice of control group and 9 mice of treatment group. Group A (K-) given pellets, group B (K+) given pellets and aspirin 150 mg/kgBB for 7 days, group C given pellets, aspirin 150 mg/kgBB and cinnamon extract 3 mg for 3 days, group D given pellets, aspirin 150 mg/kgBB for 7 days and given cinnamon extract 3 mg for 3 days, group E given pellets, aspirin 150 mg/kgBB for 7 days and given pellets only (without treatment) for 3 days. The results showed that aspirin cause gastric mucosa damage in group B (K+) compared with kelompok A (K-). Group C and D showed less inflammatory cells compared with group B (K+). Group E showed more inflammatory cells compared with group D. The study suggested that cinnamon extract has protective and therapeutic effects on gastric mucosa of Wistar rats. Keywords: cinnamon, gaster, aspirin.   Abstrak: Kayu manis (Cinnamomum burmannii) merupakan tanaman herbal tradisional yang banyak dijumpai dalam kehidupan sehari- hari dan memiliki banyak manfaat termasuk dalam bidang kesehatan. Penelitian ini bertujuan untuk mengetahui apakah ada efek pemberian kayu manis terhadap mukosa lambung yang diberi aspirin. Desain Penelitian yang digunakan adalah metode eksperimental dan subjek penelitian menggunakan tikus Wistar, yang terbagi atas 6 ekor kontrol dan 9 ekor perlakuan. Kelompok A (K-) diberikan pelet selama 7 hari, kelompok B (K+) diberikan pelet dan aspirin 150mg/kgBB selama 7 hari, kelompok C diberikan pelet, aspirin 150 mg/kgBB, ekstrak kayu manis 3 mg secara bersama- sama selama 7 hari, kelompok D diberikan pelet dan Aspirin 150 mg/kgBB selama 7 hari dilanjutkan dengan pemberian ekstrak kayu manis 3 mg selama 3 hari, kelompok E diberikan pelet dan Aspirin 150 mg/kgBB selama 7 hari dilanjutkan dengan pemberian pelet saja (tanpa perlakuan) selama 3 hari. Hasilnya menunjukkan aspirin menimbulkan kerusakan mukosa lambung pada kelompok B (K+) dibandingkan dengan kelompok A (K-). Kelompok C dan D menunjukkan sel-sel radang yang lebih sedikit dari kelompok B(kontrol +). Kelompok E menunjukkan sel- sel radang yang lebih banyak dibandingkan dengan kelompok D. Kesimpulan dari penelitian ini adalah kayu manis mempunyai efek protektif dan terapeutik terhadap mukosa lambung tikus Wistar. Kata kunci: kayu manis, lambung, aspirin.

scholarly journals GAMBARAN HISTOPATOLOGI LAMBUNG TIKUS WISTAR (Rattus norvegicus) YANG DIBERIKAN LENGKUAS (Alpinia galanga Willd) SETELAH DIINDUKSI OLEH ASAM MEFENAMAT

2013 ◽  
Vol 1 (1) ◽  
Author(s):  
Juita Pasaribu ◽  
Lily Loho ◽  
Poppy Lintong
Keyword(s):  
Wistar Rats ◽  
Acute Inflammation ◽  
Inflammatory Cells ◽  
Control Group ◽  
Treatment Groups ◽  
Acute Gastritis ◽  
Group A ◽  
Mucosal Lining ◽  
Group B ◽  
Group D

Abstrak: Gastritis akut merupakan proses inflamasi yang bersifat akut dan biasanya terjadi pada bagian permukaan mukosa lambung. Penyakit ini biasanya disebabkan karena banyak faktor salah satunya yaitu penggunaan asam mefenamat dalam dosis yang berlebihan. Pada penelitian yang telah dilakukan sebelumnya didapatkan bahwa lengkuas dapat mengurangi terjadinya radang akut. Tujuan penelitian ini adalah untuk mengetahui gambaran histopatologi lambung tikus wistar yang diberikan lengkuas setelah diinduksi dengan asam mefenamat. Penelitian ini menggunakan metode eksperimental dengan sampel 11 ekor tikus wistar yang dibagi dalam 4 kelompok perlakuan. Kelompok A tidak diberikan perlakuan. Kelompok B diberikan asam mefenamat 10 mg selama 7 hari. Kelompok C diberikan asam mefenamat 10 mg selama 7 hari kemudian diberikan perasan lengkuas selama 7 hari. Kelompok D diberikan asam mefenamat 10 mg kemudian tidak diberikan perlakuan selama 7 hari. Hasil penelitian ini menunjukkan bahwa gambaran mikroskopik lambung tikus wistar pada kelompok C terdapat sel-sel radang PMN yang lebih sedikit dibandingkan dengan kelompok B dan D. Simpulan: lengkuas dapat mengurangi sel-sel radang PMN pada mukosa lambung yang diberikan dengan asam mefenamat. Kata kunci: Lengkuas, Asam Mefenamat, Gastritis akut.   Abstract : acute gastritis is an acute inflammatory process normally occurring in the mucosal lining of the stomach. This disease is caused by a variety of factors, one of which is the use of mefenamat acid in large doses. Previous research has revealed that galangal can be used to reduce acute inflammation. The objective of this research was to reveal the histopathological pictures of stomach of wistar rats that treatment with galangal after induce by mefenamat acid. This study is an experimental, employing 11 wistar rats which were assigned for four treatment groups. Group A was the control group in which the rats received no treatment. Rats in group B were administered mefenamat acid 10 mg for 7 days. In group C, the rats were also administered mefenamat acid 10 mg for 7 days and then received galangal distillation for 7 days. In group D, rats were administered mefenamat acid 10 mg for 7 days and then for 7 days without receiving anymore mefenamat acid or galangal distillation. Results showed that microscopic pictures of stomach of wistar rats in group C were presented with PMN inflammatory cells fewer than rats in group B and D. Conclusion: galangal can reduce PMN inflammatory cells in mucosal lining of the stomach exposed to mefenamat acid. Key words: galangal, mefenamat acid,  acute gastritis.

scholarly journals The protective effect of Zinc and Magnesium against subchronic Cadmium toxicity in Wistar rats (Biochemical and neurobehavioral effects)

2019 ◽  
pp. 217-225
Author(s):  
Djemli Samir
Keyword(s):  
Blood Cell ◽  
Open Field ◽  
Biochemical Parameters ◽  
Wistar Rats ◽  
Cadmium Toxicity ◽  
Control Group ◽  
Cadmium Poisoning ◽  
Behavioral Parameters ◽  
Group A ◽  
Cadmium Zinc

Objective: The present study shows the beneficial effect of (zinc 10mg / kg + magnesium 10mg / kg) against cadmium poisoning at a dose of 15mg / kg on biochemical parameters and neurobehavioral functions in Wistar rats during a period of 45 days. Methods: The experiment was carried out on 20 Wistar rats, weighing 180 to 232g for an initial weight before starting treatment with cadmium. The rats are grouped in cages at a rate of 5 in groups:Group (1) control: was supplied only with waterGroup (2) contaminated with cadmium (Cd):  water plus oral administration cadmium dose 15mg / Kg / l.Group (3) contaminated with (Cd) dose 15mg / Kg / l in water plus magnesium (Mg) dose 10mg / Kg / l oral administration.Group (4) contaminated with (Cd) dose 15mg / Kg / l in water plus zinc (Zn) dose 10mg / Kg / l oral administration.Results: The rats exposure to cadmium showed a very highly significant decrease in body weight of cadmium-contaminated rats (P <0.001) compared with the control group. Regarding the biochemical parameters, there was a very highly significant increase (P <0.001) in the cadmium group blood glucose level compared to the control group, a highly significant increase (P <0.01) in the group urea level. cadmium compared to the control, a very highly significant increase (P <0.001) in the creatinine level of the cadmium lot compared to the control, a very highly significant increase (P <0.001) of transaminases (GOT, GPT) of the group cadmium relative to the control, complete blood  count (CBC) demonstrated a very highly significant increase (P <0.001) in the white blood cell count, the hematocrit of the cadmium lot compared to the control, a very highly significant decrease (P <0.001). ) the red blood cell and hemoglobin levels of the cadmium group compared to the control. A significant improvement (P <0.001) (P <0.01) (P <0.05) of lots: zinc, magnesium, cadmium + zinc and cadmium + zinc compared to cadmium. However, the results obtained from the neurobehavioral tests reveal a significant elevation (P <0.001) (P <0.01) (P <0.05) in the number of cells crossed, the number of straightening in the test. Open field in the group exposed to cadmium resulting in locomotors hyperactivity compared to the control and it is minimal in other groups treated with zinc and magnesium. On the other hand, the plus maze (labyrinth test) revealed a very highly significant increase (P <0.001) (P <0.01) (P <0.05) of the time spent in the closed arms of the cadmium group compared to the control, a significant improvement (P <0.001) (P <0.01) (P <0.05) of the lots: zinc, magnesium, cadmium + zinc and cadmium + zinc compared with cadmium. Conclusion: Subchronic cadmium toxicity has harmful effects on the biochemical, hematological and neuro-behavioral parameters of  Wistar rats, with the installation of an anxiety that will lead to a depressive state, which will be reduced and improved by the antioxidant effect of zinc + magnesium. Keywords: Cadmium-zinc-magnesium-subchronic toxicity-Wistar-Open field-plus maze 

Interactive effects of alcohol and chloroquine on hematologic profile of Wistar rats

2018 ◽  
Vol 29 (5) ◽  
pp. 537-543
Author(s):  
Dimkpa Uchechukwu ◽  
Kelechi Loveth Ezeudensi ◽  
Babatunde Ishola Adejumo ◽  
Chinwe Obianuju Ewenighi ◽  
Elizabeth Enohnyaket Besong ◽  
...  
Keyword(s):  
Folic Acid ◽  
Adverse Effects ◽  
Blood Cell ◽  
Cell Volume ◽  
Wistar Rats ◽  
Control Group ◽  
Vitamin B ◽  
Concurrent Administration ◽  
Mean Cell Volume ◽  
Group D

AbstractBackgroundThere is paucity of information on the adverse effects of alcohol – chloroquine interaction on hematological parameters. To investigate the effects of concurrent administration of chloroquine and ethanol on hematologic parameters of adult Wistar rats and the ameliorative role of vitamin B12and folic acid supplementation on any adverse effects.MethodsSome 30 adult Wistar rats weighing 120–200 g were assigned to six major groups of five rats each according to their weights. The control group A was fed with normal rat chow and water. The experimental groups B–F were administered with drugs for a period of 7 days as follows: B (chloroquine only); C (ethanol only); D (chloroquine+ethanol); E (chloroquine+ethanol+vitamin B12); and F (chloroquine+ethanol+vitamin B12+folic acid). Blood samples were collected from each animal by cardiac puncture to determine red blood cell (RBC) count, white blood cell (WBC) count, packed cell volume (PCV), hemoglobin (Hb), mean corpuscular hemoglobin concentration, mean cell volume (MCV), and mean cell hemoglobin (MCH). Liver enzymes, aspartate aminotransferase (AST), and alanine aminotransferase (ALT) were analyzed to test for liver function.ResultsGroup D indicated lower PCV, Hb, MCV, MCH, and greater WBC compared with control. Group E indicated greater (p<0.05) PCV, Hb, and RBC compared with Group D. Greater (p<0.05) MCV and MCH, and lower WBC and RBC were observed in Group F compared with Group D. Furthermore, Group D rats indicated greater (p<0.05) mean AST and ALT compared with Groups A, E, and F.ConclusionsConcurrent administration of chloroquine and alcohol indicated adverse effects on hematologic parameters of Wistar rats. The administration of vitamin B12and folic acid appeared to ameliorate these adverse effects.

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