Modification of a nitrocellulose membrane with cellulose nanofibers for enhanced sensitivity of lateral flow assays: application to the determination of Staphylococcus aureus

2019 ◽  
Vol 186 (12) ◽  
Author(s):  
Rui Hua Tang ◽  
Li Na Liu ◽  
Su Feng Zhang ◽  
Ang Li ◽  
Zedong Li
Keyword(s):  
Staphylococcus Aureus ◽  
Cellulose Nanofibers ◽  
Lateral Flow ◽  
Nitrocellulose Membrane ◽  
Enhanced Sensitivity ◽  
Lateral Flow Assays

Rapid lateral flow assays based on the quantification of magnetic nanoparticle labels for multiplexed immunodetection of small molecules: application to the determination of drugs of abuse

2019 ◽  
Vol 186 (9) ◽  
Author(s):  
Natalia V. Guteneva ◽  
Sergey L. Znoyko ◽  
Alexey V. Orlov ◽  
Maxim P. Nikitin ◽  
Petr I. Nikitin
Keyword(s):  
Small Molecules ◽  
Magnetic Nanoparticle ◽  
Drugs Of Abuse ◽  
Lateral Flow ◽  
Lateral Flow Assays

scholarly journals A hemagglutination-based, semi-quantitative test for point-of-care determination of SARS-CoV-2 antibody levels

2021 ◽  
Author(s):  
Robert L. Kruse ◽  
Yuting Huang ◽  
Alyssa Lee ◽  
Xianming Zhu ◽  
Ruchee Shrestha ◽  
...  
Keyword(s):  
Point Of Care ◽  
Neutralizing Antibody ◽  
Antibody Test ◽  
Quantitative Information ◽  
Elisa Test ◽  
Lateral Flow ◽  
Plasma Samples ◽  
Hemagglutination Test ◽  
Lateral Flow Assays

Serologic, point-of-care tests to detect antibodies against SARS-CoV-2 are an important tool in the COVID-19 pandemic. The majority of current point-of-care antibody tests developed for SARS-CoV-2 rely on lateral flow assays, but these do not offer quantitative information. To address this, we developed a novel antibody test leveraging hemagglutination, employing a dry card format currently used for typing ABO blood groups. 200 COVID-19 patient and 200 control plasma samples were reconstituted with O-negative RBCs to form whole blood and added to dried viral-antibody fusion protein, followed by a stirring step and a tilting step, 3-minute incubation, and a second tilting step. The sensitivity for the hemagglutination test, Euroimmun IgG ELISA test and RBD-based CoronaChek lateral flow assay was 87.0%, 86.5%, and 84.5%, respectively, using samples obtained from recovered COVID-19 individuals. Testing pre-pandemic samples, the hemagglutination test had a specificity of 95.5%, compared to 97.3% and 98.9% for the ELISA and CoronaChek, respectively. A distribution of agglutination strengths was observed in COVID-19 convalescent plasma samples, with the highest agglutination score (4) exhibiting significantly higher neutralizing antibody titers than weak positives (2) (p<0.0001). Strong agglutinations were observed within 1 minute of testing, and this shorter assay time also increased specificity to 98.5%. In conclusion, we developed a novel rapid, point-of-care RBC agglutination test for the detection of SARS-CoV-2 antibodies that can yield semi-quantitative information on neutralizing antibody titer in patients. The five-minute test may find use in determination of serostatus prior to vaccination, post-vaccination surveillance and travel screening.

Effects of membrane cast thickness on controlling the macrovoid structure in lateral flow nitrocellulose membrane and determination of its characteristics

2007 ◽  
Vol 57 (8) ◽  
pp. 743-746 ◽  
Author(s):  
A.L. Ahmad ◽  
S.C. Low ◽  
S.R.Abd. Shukor
Keyword(s):  
Lateral Flow ◽  
Nitrocellulose Membrane

Oligonucleotide-linked gold nanoparticle aggregates for enhanced sensitivity in lateral flow assays

Lab on a Chip ◽  
2013 ◽  
Vol 13 (22) ◽  
pp. 4352 ◽  
Author(s):  
Jie Hu ◽  
Lin Wang ◽  
Fei Li ◽  
Yu Long Han ◽  
Min Lin ◽  
...  
Keyword(s):  
Gold Nanoparticle ◽  
Lateral Flow ◽  
Enhanced Sensitivity ◽  
Nanoparticle Aggregates ◽  
Lateral Flow Assays

Gold-Aptamer-Nanoconstructs Engineered to Detect Conserved Enteroviral Nucleic Acid Sequences

2019 ◽  
Author(s):  
Veeren Chauhan ◽  
Mohamed M Elsutohy ◽  
C Patrick McClure ◽  
Will Irving ◽  
Neil Roddis ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
In Silico ◽  
Point Of Care ◽  
Lateral Flow ◽  
Nucleic Acid Sequence ◽  
In Silico Screening ◽  
Lateral Flow Assays ◽  
Life Threatening ◽  
Software And Hardware ◽  
Nucleic Acid Sequences

<p>Enteroviruses are a ubiquitous mammalian pathogen that can produce mild to life-threatening disease. Bearing this in mind, we have developed a rapid, accurate and economical point-of-care biosensor that can detect a nucleic acid sequences conserved amongst 96% of all known enteroviruses. The biosensor harnesses the physicochemical properties of gold nanoparticles and aptamers to provide colourimetric, spectroscopic and lateral flow-based identification of an exclusive enteroviral RNA sequence (23 bases), which was identified through in silico screening. Aptamers were designed to demonstrate specific complementarity towards the target enteroviral RNA to produce aggregated gold-aptamer nanoconstructs. Conserved target enteroviral nucleic acid sequence (≥ 1x10<sup>-7</sup> M, ≥1.4×10<sup>-14</sup> g/mL), initiates gold-aptamer-nanoconstructs disaggregation and a signal transduction mechanism, producing a colourimetric and spectroscopic blueshift (544 nm (purple) > 524 nm (red)). Furthermore, lateral-flow-assays that utilise gold-aptamer-nanoconstructs were unaffected by contaminating human genomic DNA, demonstrated rapid detection of conserved target enteroviral nucleic acid sequence (< 60 s) and could be interpreted with a bespoke software and hardware electronic interface. We anticipate our methodology will translate in-silico screening of nucleic acid databases to a tangible enteroviral desktop detector, which could be readily translated to related organisms. This will pave-the-way forward in the clinical evaluation of disease and complement existing strategies at overcoming antimicrobial resistance.</p>

scholarly journals Dual-signal lateral flow assay using vancomycin-modified nanotags for rapid and sensitive detection of Staphylococcus aureus

RSC Advances ◽  
2021 ◽  
Vol 11 (22) ◽  
pp. 13297-13303
Author(s):  
Shu Wang ◽  
Wanzhu Shen ◽  
Shuai Zheng ◽  
Zhigang Li ◽  
Chongwen Wang ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Lateral Flow ◽  
Lateral Flow Assay ◽  
Sensitive Detection

A colorimetric-fluorescent dual-signal lateral flow assay was proposed for the sensitive detection of S. aureus by using vancomycin-modified SiO2–Au-QD tags.

Inductively coupled plasma optical emission spectroscopy as a tool for evaluating lateral flow assays

2021 ◽  
Author(s):  
Jenna M. DeSousa ◽  
Micaella Z. Jorge ◽  
Hayley B. Lindsay ◽  
Frederick R. Haselton ◽  
David W. Wright ◽  
...  
Keyword(s):  
Inductively Coupled Plasma ◽  
Emission Spectroscopy ◽  
Optical Emission Spectroscopy ◽  
Optical Emission ◽  
Lateral Flow ◽  
Gold Content ◽  
Icp Oes ◽  
Inductively Coupled ◽  
Lateral Flow Assays ◽  
Plasma Optical Emission Spectroscopy

This work demonstrates the first use of ICP-OES to quantitatively analyze gold content on lateral flow assays.

scholarly journals Integrating high-performing electrochemical transducers in lateral flow assay

2021 ◽  
Author(s):  
Antonia Perju ◽  
Nongnoot Wongkaew
Keyword(s):  
High Performance ◽  
Point Of Care ◽  
Low Cost ◽  
High Sensitivity ◽  
Lateral Flow ◽  
Analytical Performance ◽  
Label Free ◽  
High Performing ◽  
Lateral Flow Assays ◽  
Electrochemical Transducers

AbstractLateral flow assays (LFAs) are the best-performing and best-known point-of-care tests worldwide. Over the last decade, they have experienced an increasing interest by researchers towards improving their analytical performance while maintaining their robust assay platform. Commercially, visual and optical detection strategies dominate, but it is especially the research on integrating electrochemical (EC) approaches that may have a chance to significantly improve an LFA’s performance that is needed in order to detect analytes reliably at lower concentrations than currently possible. In fact, EC-LFAs offer advantages in terms of quantitative determination, low-cost, high sensitivity, and even simple, label-free strategies. Here, the various configurations of EC-LFAs published are summarized and critically evaluated. In short, most of them rely on applying conventional transducers, e.g., screen-printed electrode, to ensure reliability of the assay, and additional advances are afforded by the beneficial features of nanomaterials. It is predicted that these will be further implemented in EC-LFAs as high-performance transducers. Considering the low cost of point-of-care devices, it becomes even more important to also identify strategies that efficiently integrate nanomaterials into EC-LFAs in a high-throughput manner while maintaining their favorable analytical performance.

scholarly journals Utility of urine and serum lateral flow assays to determine the prevalence and predictors of cryptococcal antigenemia in HIV-positive outpatients beginning antiretroviral therapy in Mwanza, Tanzania

2014 ◽  
Vol 17 (1) ◽  
pp. 19040 ◽  
Author(s):  
Kinanga A Magambo ◽  
Samuel E Kalluvya ◽  
Shikha W Kapoor ◽  
Jeremiah Seni ◽  
Awilly A Chofle ◽  
...  
Keyword(s):  
Antiretroviral Therapy ◽  
Lateral Flow ◽  
Hiv Positive ◽  
Lateral Flow Assays ◽  
Urine And Serum
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