A sandwich-configuration electrochemiluminescence immunoassay based on Cu2O@OMC-Ru nanocrystals and OMC-MoS2 nanocomposites for determination of alpha-fetoprotein

Abstract The CA 125 II assay on the Elecsys® 2010 analyzer was evaluated in an international multicenter trial. Imprecision studies yielded within-run CVs of 0.8–3.3% and between-day CVs of 2.4–10.9%; CVs for total imprecision in the manufacturer’s laboratory were 2.4–7.8%. The linear range of the assay extended to at least 4500 kilounits/L (three decades). Interference from triglycerides (10.3 mmol/L), bilirubin (850 μmol/L), hemoglobin (1.1 mmol/L), anticoagulants (plasma), and several widely used drugs was undetectable. Method comparisons with five other CA 125 II assays showed good correlation but differences in standardization. A 95th percentile cutoff value of 35 kilounits/L was calculated from values measured in 593 apparently healthy (pre- and postmenopausal) women. In 95% of patients with benign gynecological diseases CA 125 was ≤190 kilounits/L; 63% of patients with newly diagnosed ovarian carcinoma had values >190 kilounits/L. A comparison of CA 125 values obtained with the Elecsys test and with other common CA 125 tests in monitored patients being treated for ovarian cancer showed identical patterns. In conclusion, the Elecsys CA 125 II assay is linear over a broad range, yields precise and accurate results, is free from interferences, and compares well with other assays.

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Early Diagnosis of Trophoblastic Disease and Fetal Maldevelopment by Determination of Alpha-Fetoprotein (AFP), Human Chorionic Gonadotrophin (HCG) and Amniography

Acta Obstetricia Et Gynecologica Scandinavica ◽

10.3109/00016347709157214 ◽

1977 ◽

Vol 56 (s69) ◽

pp. 83-93 ◽

Cited By ~ 5

Author(s):

B. Kjessler ◽

A. Hemmingsson ◽

B. A. Nilsson ◽

S. G. O. Johansson

Keyword(s):

Early Diagnosis ◽

Chorionic Gonadotrophin ◽

Human Chorionic Gonadotrophin ◽

Alpha Fetoprotein ◽

Trophoblastic Disease

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Automated particle-counting immunoassay for alpha-fetoprotein.

Clinical Chemistry ◽

10.1093/clinchem/27.1.64 ◽

1981 ◽

Vol 27 (1) ◽

pp. 64-67 ◽

Cited By ~ 15

Author(s):

D Collet-Cassart ◽

C G Magnusson ◽

J G Ratcliffe ◽

C L Cambiaso ◽

P L Masson

Keyword(s):

Correlation Coefficient ◽

Immunoglobulin G ◽

Incubation Time ◽

Blood Cells ◽

Sampling Rate ◽

Alpha Fetoprotein ◽

Optical Cell ◽

Standard Curve ◽

Cell Counter

Abstract PACIA, a homogeneous non-radioimmunoassay, has been adapted to the determination of serum alpha 1-fetoprotein. This technique is based on the agglutination of latex particles coated with antibodies to the antigen to be determined. The agglutination is measured by using an optical cell counter designed to count blood cells, to determine the reduction in the number of non-agglutinated particles. Interferences by serum constituents are avoided by coating the particles with the F(ab')2-fragments of th immunoglobulin G fraction of the antiserum. The system is automated, with a sampling rate of 50/h and an incubation time of 26 min. Concentrations used in preparing the standard curve ranged from 1 to 50 microgram/L; analytical recoveries were 93.5 to 98.4%; the correlation coefficient of PACIA with radioimmunoassay, calculated from results on 127 samples, was 0.98; maximum within- and between-assay CVs were 7.4% and 9.6%, respectively.

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