A simple strategy for signal enhancement in lateral flow assays using superabsorbent polymers

2021 ◽  
Vol 188 (11) ◽  
Author(s):  
Taeyeong You ◽  
Woojin Jeong ◽  
Hwankyu Lee ◽  
Yun Suk Huh ◽  
Sun Min Kim ◽  
...  
Keyword(s):  
Signal Enhancement ◽  
Lateral Flow ◽  
Superabsorbent Polymers ◽  
Simple Strategy ◽  
Lateral Flow Assays

Yersinia pestis detection using biotinylated dNTPs for signal enhancement in lateral flow assays

2020 ◽  
Vol 1112 ◽  
pp. 54-61 ◽  
Author(s):  
Salah Kortli ◽  
Miriam Jauset-Rubio ◽  
Herbert Tomaso ◽  
Mohammed Nooredeen Abbas ◽  
Abdulaziz S. Bashammakh ◽  
...  
Keyword(s):  
Yersinia Pestis ◽  
Signal Enhancement ◽  
Lateral Flow ◽  
Lateral Flow Assays

Gold-Aptamer-Nanoconstructs Engineered to Detect Conserved Enteroviral Nucleic Acid Sequences

2019 ◽  
Author(s):  
Veeren Chauhan ◽  
Mohamed M Elsutohy ◽  
C Patrick McClure ◽  
Will Irving ◽  
Neil Roddis ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
In Silico ◽  
Point Of Care ◽  
Lateral Flow ◽  
Nucleic Acid Sequence ◽  
In Silico Screening ◽  
Lateral Flow Assays ◽  
Life Threatening ◽  
Software And Hardware ◽  
Nucleic Acid Sequences

<p>Enteroviruses are a ubiquitous mammalian pathogen that can produce mild to life-threatening disease. Bearing this in mind, we have developed a rapid, accurate and economical point-of-care biosensor that can detect a nucleic acid sequences conserved amongst 96% of all known enteroviruses. The biosensor harnesses the physicochemical properties of gold nanoparticles and aptamers to provide colourimetric, spectroscopic and lateral flow-based identification of an exclusive enteroviral RNA sequence (23 bases), which was identified through in silico screening. Aptamers were designed to demonstrate specific complementarity towards the target enteroviral RNA to produce aggregated gold-aptamer nanoconstructs. Conserved target enteroviral nucleic acid sequence (≥ 1x10<sup>-7</sup> M, ≥1.4×10<sup>-14</sup> g/mL), initiates gold-aptamer-nanoconstructs disaggregation and a signal transduction mechanism, producing a colourimetric and spectroscopic blueshift (544 nm (purple) > 524 nm (red)). Furthermore, lateral-flow-assays that utilise gold-aptamer-nanoconstructs were unaffected by contaminating human genomic DNA, demonstrated rapid detection of conserved target enteroviral nucleic acid sequence (< 60 s) and could be interpreted with a bespoke software and hardware electronic interface. We anticipate our methodology will translate in-silico screening of nucleic acid databases to a tangible enteroviral desktop detector, which could be readily translated to related organisms. This will pave-the-way forward in the clinical evaluation of disease and complement existing strategies at overcoming antimicrobial resistance.</p>

Inductively coupled plasma optical emission spectroscopy as a tool for evaluating lateral flow assays

2021 ◽  
Author(s):  
Jenna M. DeSousa ◽  
Micaella Z. Jorge ◽  
Hayley B. Lindsay ◽  
Frederick R. Haselton ◽  
David W. Wright ◽  
...  
Keyword(s):  
Inductively Coupled Plasma ◽  
Emission Spectroscopy ◽  
Optical Emission Spectroscopy ◽  
Optical Emission ◽  
Lateral Flow ◽  
Gold Content ◽  
Icp Oes ◽  
Inductively Coupled ◽  
Lateral Flow Assays ◽  
Plasma Optical Emission Spectroscopy

This work demonstrates the first use of ICP-OES to quantitatively analyze gold content on lateral flow assays.

scholarly journals Integrating high-performing electrochemical transducers in lateral flow assay

2021 ◽  
Author(s):  
Antonia Perju ◽  
Nongnoot Wongkaew
Keyword(s):  
High Performance ◽  
Point Of Care ◽  
Low Cost ◽  
High Sensitivity ◽  
Lateral Flow ◽  
Analytical Performance ◽  
Label Free ◽  
High Performing ◽  
Lateral Flow Assays ◽  
Electrochemical Transducers

AbstractLateral flow assays (LFAs) are the best-performing and best-known point-of-care tests worldwide. Over the last decade, they have experienced an increasing interest by researchers towards improving their analytical performance while maintaining their robust assay platform. Commercially, visual and optical detection strategies dominate, but it is especially the research on integrating electrochemical (EC) approaches that may have a chance to significantly improve an LFA’s performance that is needed in order to detect analytes reliably at lower concentrations than currently possible. In fact, EC-LFAs offer advantages in terms of quantitative determination, low-cost, high sensitivity, and even simple, label-free strategies. Here, the various configurations of EC-LFAs published are summarized and critically evaluated. In short, most of them rely on applying conventional transducers, e.g., screen-printed electrode, to ensure reliability of the assay, and additional advances are afforded by the beneficial features of nanomaterials. It is predicted that these will be further implemented in EC-LFAs as high-performance transducers. Considering the low cost of point-of-care devices, it becomes even more important to also identify strategies that efficiently integrate nanomaterials into EC-LFAs in a high-throughput manner while maintaining their favorable analytical performance.

scholarly journals Quantitative Lateral Flow Assays for Salivary Biomarker Assessment: A Review

2017 ◽  
Vol 5 ◽  
Author(s):  
Olga Miočević ◽  
Craig R. Cole ◽  
Mary J. Laughlin ◽  
Robert L. Buck ◽  
Paul D. Slowey ◽  
...  
Keyword(s):  
Lateral Flow ◽  
Salivary Biomarker ◽  
Lateral Flow Assays

An improved detection limit and working range of lateral flow assays based on a mathematical model

The Analyst ◽  
2018 ◽  
Vol 143 (12) ◽  
pp. 2775-2783 ◽  
Author(s):  
Zhi Liu ◽  
Zhiguo Qu ◽  
Ruihua Tang ◽  
Xiaocong He ◽  
Hui Yang ◽  
...  
Keyword(s):  
Mathematical Model ◽  
Detection Limit ◽  
Lateral Flow ◽  
Lateral Flow Assays

The detection limit and working range of lateral flow assays are investigated experimentally and numerically.

Rapid Prototyping of Lateral Flow Assays

2009 ◽  
pp. 217-235 ◽  
Author(s):  
Alexander Volkov ◽  
Michael Mauk ◽  
Paul Corstjens ◽  
R. Sam Niedbala
Keyword(s):  
Rapid Prototyping ◽  
Lateral Flow ◽  
Lateral Flow Assays
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