scholarly journals A fast and simple one-step duplex PCR assay for canine distemper virus (CDV) and canine coronavirus (CCoV) detection

2018 ◽  
Vol 163 (12) ◽  
pp. 3345-3349 ◽  
Author(s):  
Jing Wang ◽  
Yakun Luo ◽  
Lin Liang ◽  
Jinxiang Li ◽  
Shangjin Cui
Keyword(s):  
Canine Distemper Virus ◽  
Duplex Pcr ◽  
Canine Distemper ◽  
Pcr Assay ◽  
Canine Coronavirus ◽  
One Step ◽  
Duplex Pcr Assay

scholarly journals Duplex-PCR assay for the detection of adenovirus and respiratory syncytial virus in nasopharyngeal samples

2009 ◽  
Vol 104 (1) ◽  
pp. 118-120 ◽  
Author(s):  
Juliana Cristina Marinheiro ◽  
Roberta Braga Sanalios ◽  
Daniela Carvalho dos Santos ◽  
Cristovão Alves da Costa ◽  
Charlotte Marianna Hársi
Keyword(s):  
Respiratory Syncytial Virus ◽  
Duplex Pcr ◽  
Pcr Assay ◽  
Syncytial Virus ◽  
Duplex Pcr Assay

Rapid detection of Yersinia enterocolitica serotype O:3 using a duplex PCR assay

2018 ◽  
Vol 154 ◽  
pp. 107-111 ◽  
Author(s):  
Leonardo Alves Rusak ◽  
Rodrigo de Castro Lisboa Pereira ◽  
Isabelle Geoffroy Freitag ◽  
Cristina Barroso Hofer ◽  
Ernesto Hofer ◽  
...  
Keyword(s):  
Yersinia Enterocolitica ◽  
Rapid Detection ◽  
Duplex Pcr ◽  
Pcr Assay ◽  
Serotype O ◽  
Duplex Pcr Assay

scholarly journals A New Molecular Detection System for Canine Distemper Virus Based on a Double-Check Strategy

Viruses ◽  
2021 ◽  
Vol 13 (8) ◽  
pp. 1632
Author(s):  
Sabrina Halecker ◽  
Sabine Bock ◽  
Martin Beer ◽  
Bernd Hoffmann
Keyword(s):  
Canine Distemper Virus ◽  
Parallel Implementation ◽  
Detection System ◽  
Quantitative Polymerase Chain Reaction ◽  
Diagnostic Methods ◽  
Molecular Diagnostic ◽  
Wild Animals ◽  
Canine Distemper ◽  
One Step ◽  
Diagnostic Sensitivity And Specificity

Due to changing distemper issues worldwide and to inadequate results of an inter-laboratory study in Germany, it seems sensible to adapt and optimize the diagnostic methods for the detection of the canine distemper virus (CDV) to the new genetic diversity of virus strains. The goal of the project was the development, establishment and validation of two independent one-step reverse transcriptase quantitative polymerase chain reaction (RT-qPCR) methods for the safe detection of CDV in domestic and wild animals. For this purpose, an existing CDV-RT-qPCR was decisively adapted and, in addition, a completely new system was developed. Both CDV-RT-qPCR systems are characterized by a very high, comparable analytical and diagnostic sensitivity and specificity and can be mutually combined with inhibition or extraction controls. The reduction in the master mix used allows for the parallel implementation of both CDV-RT-qPCR systems without significant cost increases. For validation of the new CDV-RT-qPCR duplex assays, a panel comprising 378 samples derived from Germany, several European countries and one African country were tested. A sensitivity of 98.9% and a specificity of 100% were computed for the new assays, thus being a reliable molecular diagnostic tool for the detection of CDV in domestic and wild animals.

A duplex PCR assay for authentication of Ocimum basilicum L. and Ocimum tenuiflorum L in Tulsi churna

Food Control ◽  
2021 ◽  
pp. 108790
Author(s):  
Tasnim Travadi ◽  
Sonal Sharma ◽  
Ramesh Pandit ◽  
Mital Nakrani ◽  
Chaitanya Joshi ◽  
...  
Keyword(s):  
Ocimum Basilicum ◽  
Duplex Pcr ◽  
Pcr Assay ◽  
Ocimum Tenuiflorum ◽  
Duplex Pcr Assay

scholarly journals Detection of Trypanosoma cruzi and Trypanosoma rangeli Infection by Duplex PCR Assay Based on Telomeric Sequences

2003 ◽  
Vol 10 (5) ◽  
pp. 775-779 ◽  
Author(s):  
Miguel Angel Chiurillo ◽  
Gladys Crisante ◽  
Agustina Rojas ◽  
Andreina Peralta ◽  
Manuel Dias ◽  
...  
Keyword(s):  
Trypanosoma Cruzi ◽  
Human Subjects ◽  
Simultaneous Detection ◽  
Duplex Pcr ◽  
Trypanosoma Rangeli ◽  
Pcr Assay ◽  
Telomeric Sequences ◽  
Species Specific ◽  
Duplex Pcr Assay ◽  
Reduviid Bugs

ABSTRACT We used the species specificity and repetitious nature of subtelomeric kinetoplastida sequences to generate a duplex PCR assay for the simultaneous detection of Trypanosoma cruzi and Trypanosoma rangeli in experimentally and naturally infected triatomine (Reduviid) bugs and in infected human subjects. The assay was species specific and was capable of detecting 1/20th of T. cruzi and 1/4th of T. rangeli cell equivalents without complementary hybridization. In addition, the PCR-based assay was robust enough for direct application to difficult biological samples such as Reduviid feces or guts and was capable of recognizing all T. cruzi and T. rangeli strains and lineages. Because the assay primers amplify entirely different target sequences, no reaction interference was observed, facilitating future adaptation of this assay to an automated format.

scholarly journals Development and characterization of a duplex PCR assay in Chinese sturgeon (Acipenser sinensis) for genetic analysis

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Yacheng Hu ◽  
Xueqing Liu ◽  
Jing Yang ◽  
Kan Xiao ◽  
Binzhong Wang ◽  
...  
Keyword(s):  
Genetic Analysis ◽  
Duplex Pcr ◽  
Chinese Sturgeon ◽  
Pcr Assay ◽  
Acipenser Sinensis ◽  
Duplex Pcr Assay

scholarly journals Canine distemper virus detection by different methods of One-Step RT-qPCR

2016 ◽  
Vol 46 (9) ◽  
pp. 1601-1606
Author(s):  
Claudia de Camargo Tozato ◽  
Vívian Ferreira Zadra ◽  
Caroline Rodrigues Basso ◽  
João Pessoa Araújo Junior
Keyword(s):  
Urine Sample ◽  
Canine Distemper Virus ◽  
Limit Of Detection ◽  
Analytical Sensitivity ◽  
Canine Distemper ◽  
System A ◽  
Virus Rna ◽  
One Step ◽  
Commercial Kits ◽  
The One

ABSTRACT: Three commercial kits of One-Step RT-qPCR were evaluated for the molecular diagnosis of Canine Distemper Virus. Using the kit that showed better performance, two systems of Real-time RT-PCR (RT-qPCR) assays were tested and compared for analytical sensitivity to Canine Distemper Virus RNA detection: a One-Step RT-qPCR (system A) and a One-Step RT-qPCR combined with NESTED-qPCR (system B). Limits of detection for both systems were determined using a serial dilution of Canine Distemper Virus synthetic RNA or a positive urine sample. In addition, the same urine sample was tested using samples with prior centrifugation or ultracentrifugation. Commercial kits of One-Step RT-qPCR assays detected canine distemper virus RNA in 10 (100%) urine samples from symptomatic animals tested. The One-Step RT-qPCR kit that showed better results was used to evaluate the analytical sensitivity of the A and B systems. Limit of detection using synthetic RNA for the system A was 11 RNA copies µL-1 and 110 RNA copies µl-1 for first round System B. The second round of the NESTED-qPCR for System B had a limit of detection of 11 copies µl-1. Relationship between Ct values and RNA concentration was linear. The RNA extracted from the urine dilutions was detected in dilutions of 10-3 and10-2 by System A and B respectively. Urine centrifugation increased the analytical sensitivity of the test and proved to be useful for routine diagnostics. The One-Step RT-qPCR is a fast, sensitive and specific method for canine distemper routine diagnosis and research projects that require sensitive and quantitative methodology.

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