Ivermectin effectively inhibits hepatitis E virus replication, requiring the host nuclear transport protein importin α1

AbstractWe show that ivermectin, an FDA-approved anti-parasitic drug, effectively inhibits infection with hepatitis E virus (HEV) genotypes 1 and 3 in a range of cell culture models, including hepatic and extrahepatic cells. Long-term treatment showed no clear evidence of the development of drug resistance. Gene silencing of importin-α1, a cellular target of ivermectin and a key member of the host nuclear transport complex, inhibited viral replication and largely abolished the anti-HEV effect of ivermectin.

Download Full-text

Ribavirin long-term treatment for chronic hepatitis E virus infection in a liver transplant recipient

Digestive and Liver Disease ◽

10.1016/j.dld.2020.05.044 ◽

2020 ◽

Vol 52 (8) ◽

pp. 926-927 ◽

Cited By ~ 1

Author(s):

M Casper ◽

MC Reichert ◽

J Rissland ◽

F Grünhage ◽

F Lammert

Keyword(s):

Chronic Hepatitis ◽

Virus Infection ◽

Transplant Recipient ◽

Liver Transplant ◽

Hepatitis E Virus ◽

Liver Transplant Recipient ◽

Hepatitis E ◽

Term Treatment ◽

Long Term Treatment

Download Full-text

The macrolide antibiotic azithromycin potently inhibits hepatitis E virus in cell culture models

International Journal of Antimicrobial Agents ◽

10.1016/j.ijantimicag.2021.106383 ◽

2021 ◽

pp. 106383

Author(s):

Zhijiang Miao ◽

Ruyi Zhang ◽

Peifa Yu ◽

Yang Li ◽

Qiuwei Pan ◽

...

Keyword(s):

Cell Culture ◽

Hepatitis E Virus ◽

Macrolide Antibiotic ◽

Hepatitis E ◽

Culture Models ◽

Cell Culture Models

Download Full-text

Animal Models for Hepatitis E Virus

Viruses ◽

10.3390/v11060564 ◽

2019 ◽

Vol 11 (6) ◽

pp. 564 ◽

Cited By ~ 1

Author(s):

Laura Corneillie ◽

Dominic Banda ◽

Philip Meuleman

Keyword(s):

Animal Models ◽

Hepatitis E Virus ◽

Acute Viral Hepatitis ◽

Hepatitis E ◽

Medical Problem ◽

Western World ◽

Species Barrier ◽

The Past ◽

Culture Models ◽

Cell Culture Models

Hepatitis E virus (HEV) is an underdiagnosed pathogen with approximately 20 million infections each year and currently the most common cause of acute viral hepatitis. HEV was long considered to be confined to developing countries but there is increasing evidence that it is also a medical problem in the Western world. HEV that infects humans belongs to the Orthohepevirus A species of the Hepeviridae family. Novel HEV-like viruses have been observed in a variety of animals and some have been shown to be able to cross the species barrier, causing infection in humans. Several cell culture models for HEV have been established in the past years, but their efficiency is usually relatively low. With the circulation of this virus and related viruses in a variety of species, several different animal models have been developed. In this review, we give an overview of these animal models, indicate their main characteristics, and highlight how they may contribute to our understanding of the basic aspects of the viral life cycle and cross-species infection, the study of pathogenesis, and the evaluation of novel preventative and therapeutic strategies.

Download Full-text

Cell Culture Models for Hepatitis E Virus

Viruses ◽

10.3390/v11070608 ◽

2019 ◽

Vol 11 (7) ◽

pp. 608 ◽

Cited By ~ 11

Author(s):

Rebecca Menhua Fu ◽

Charlotte Caroline Decker ◽

Viet Loan Dao Thi

Keyword(s):

Cell Culture ◽

Life Cycle ◽

Hepatitis E Virus ◽

Hepatitis E ◽

Cellular Systems ◽

Advantages And Disadvantages ◽

Infectious Clones ◽

Cell Culture Systems ◽

Culture Models ◽

Cell Culture Models

Despite a growing awareness, hepatitis E virus (HEV) remains understudied and investigations have been historically hampered by the absence of efficient cell culture systems. As a result, the pathogenesis of HEV infection and basic steps of the HEV life cycle are poorly understood. Major efforts have recently been made through the development of HEV infectious clones and cellular systems that significantly advanced HEV research. Here, we summarize these systems, discussing their advantages and disadvantages for HEV studies. We further capitalize on the need for HEV-permissive polarized cell models to better recapitulate the entire HEV life cycle and transmission.

Download Full-text