Genome sequence analysis of Cronobacter phage PF-CE2 and proposal of a new species in the genus Pseudotevenvirus

2021 ◽  
Author(s):  
Mengshi Xiao ◽  
Xinmiao Ren ◽  
Ying Yu ◽  
Han Sun ◽  
Haijin Mou ◽  
...  
Keyword(s):  
New Species ◽  
Sequence Analysis ◽  
Genome Sequence ◽  
Genome Sequence Analysis ◽  
A New Species

scholarly journals Genome Sequence Analysis of Cronobacter Phage PF-CE2 and Proposal of a New Species in the Escherichia Virus RB16 Genus

2021 ◽  
Author(s):  
Mengshi Xiao ◽  
Xinmiao Ren ◽  
Ying Yu ◽  
Han Sun ◽  
Haijin Mou ◽  
...  
Keyword(s):  
New Species ◽  
Sequence Analysis ◽  
Heterologous Expression ◽  
Theoretical Basis ◽  
Taxonomic Status ◽  
Average Nucleotide Identity ◽  
Cronobacter Sakazakii ◽  
Genome Sequence Analysis ◽  
The Family ◽  
A New Species

Abstract The genome of Cronobacter sakazakii M1 phage named PF-CE2 was characterized in this work. And a new species named Cronobacter virus PF-CE2, in the Escherichia virus RB16 genus of the subfamily Tevenvirinae of the family Myoviridae was established. The Gp190 gene of phage PF-CE2 was first proposed to encode a bacteriophage-borne glycanase, which is capable of degrading fucose-containing exopolysaccharides produced by C. sakazakii M1. Further, the taxonomic status of eight additional phages was modified according to average nucleotide identity analysis. This finding provides a theoretical basis for subsequent heterologous expression of the phage PF-CE2 glycanase and provides an important reference for the preservation and sharing of these phages.

scholarly journals Recurrent Mycobacterium chelonae Skin Infection Unmasked as Factitious Disorder Using Bacterial Whole Genome Sequence Analysis

2020 ◽  
Vol 7 (11) ◽  
Author(s):  
Sarah Flohr ◽  
Alban Ramette ◽  
Philipp K A Agyeman ◽  
Andrea Duppenthaler ◽  
Cordula Scherer ◽  
...  
Keyword(s):  
Sequence Analysis ◽  
Genome Sequence ◽  
Surgical Therapy ◽  
Skin Infection ◽  
Whole Genome Sequence ◽  
Factitious Disorder ◽  
Whole Genome ◽  
Mycobacterium Chelonae ◽  
Genome Sequence Analysis ◽  
Adequate Therapy

Abstract Mycobacterium chelonae infections usually resolve with adequate therapy. We report the case of an adolescent with a chronic and progressive M chelonae infection refractory to combined antimicrobial and surgical therapy. Whole genome sequence analysis of consecutive isolates distinguished reinfection from recurrence and contributed to the diagnosis of a factitious disorder.

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