scholarly journals Evaluation of viral load and transcriptome changes in tracheal tissue of two hybrids of commercial broiler chickens infected with avian infectious bronchitis virus: a comparative study

2022 ◽  
Author(s):  
Hamzeh Ghobadian Diali ◽  
Hossein Hosseini ◽  
Mohammad Hossein Fallah Mehrabadi ◽  
Ramak Yahyaraeyat ◽  
Arash Ghalyanchilangeroudi
Keyword(s):  
Viral Load ◽  
Comparative Study ◽  
Infectious Bronchitis Virus ◽  
Broiler Chickens ◽  
Avian Infectious Bronchitis Virus ◽  
Infectious Bronchitis ◽  
Commercial Broiler ◽  
Tracheal Tissue

scholarly journals Increased expression of Interleukin-6 related to nephritis in chickens challenged with an Avian infectious bronchitis virus variant

2015 ◽  
Vol 35 (3) ◽  
pp. 216-222 ◽  
Author(s):  
Filipe S. Fernando ◽  
Cintia H. Okino ◽  
Ketherson R. Silva ◽  
Camila C. Fernandes ◽  
Mariana C.M. Gonçalves ◽  
...  
Keyword(s):  
Viral Load ◽  
Infectious Bronchitis Virus ◽  
Immunosuppressive Agents ◽  
Field Isolate ◽  
Gene Sequencing ◽  
Inflammatory Cells ◽  
Avian Infectious Bronchitis Virus ◽  
Virus Variant ◽  
Infectious Bronchitis ◽  
Inflammatory Processes

A Brazilian field isolate (IBV/Brazil/PR05) of avian infectious bronchitis virus (IBV), associated with development of nephritis in chickens, was previously genotyped as IBV variant after S1 gene sequencing. The aim of this study was to evaluate the levels of IL-6 in kidneys and trachea of birds vaccinated and challenged with IBV/Brazil/PR05 strain, correlating these results with scores of microscopic lesions, specific IBV antigen detection and viral load. The up-regulation of IL-6 and the increased levels of viral load on renal and tracheal samples were significantly correlated with scores of microscopic lesions. Reduced levels of viral load were detected in kidneys of birds previously vaccinated and challenged, compared to non-vaccinated challenged group, although markedly microscopic lesions were observed for both groups. The expression of IL-6, present both in the kidney and in the tracheas, was dependent on the load of the virus present in the tissue, and the development of lesions was related with IL-6 present in the tissues. These data suggest that variant IBV/Brazil/PR05 can induce the expression of proinflammatory cytokines in a manner correlated with viral load and increased IL-6 is involved in the tissue with the influx of inflammatory cells and subsequent nephritis. This may contribute with a model to the development of immunosuppressive agents of IL-6 to prevent acute inflammatory processes against infection with IBV and perhaps other coronaviruses, as well as contribute to the understanding of the immunopathogenesis of IBV nephropatogenic strains.

scholarly journals Pathogenesis and Tissue Distribution of Avian Infectious Bronchitis Virus Isolate IRFIBV32 (793/B Serotype) in Experimentally Infected Broiler Chickens

2012 ◽  
Vol 2012 ◽  
pp. 1-6 ◽  
Author(s):  
Zahra Boroomand ◽  
Keramat Asasi ◽  
Ali Mohammadi
Keyword(s):  
Tissue Distribution ◽  
Infectious Bronchitis Virus ◽  
Broiler Chickens ◽  
Virus Isolate ◽  
Clinical Signs ◽  
Viral Rna ◽  
Control Group ◽  
Avian Infectious Bronchitis Virus ◽  
Infectious Bronchitis ◽  
Infectious Bronchitis Virus Isolate

Infectious bronchitis (IB) is one of the most important viral diseases of poultry. The aim of this study was to investigate the distribution of avian infectious bronchitis virus isolate IRFIBV32 (793/B serotype) in experimentally infected chicken. Ninety-one-day-old commercial broilers were divided randomly into two groups (seventy in the experimental and twenty in the control group). Chicks in the experimental group were inoculated intranasally with 105ELD50/0.1 mL of the virus at three weeks of age. The samples from various tissues were collected at1, 2, 3, 5, 7, 11, 13, 15, and 20 days postinoculation. Chickens exhibited mild respiratory signs and depression. Viral RNA was detected in the kidney, lung and tracheas on days 1 to 13 PI, in the oviduct between, days 3 and 13, in testes between days 1 and 11 PI, and in the caecal tonsil consistently up to day 20 PI. The most remarkable clinical signs and virus detection appeared on day 1 PI. Data indicated that the number of infected chickens and viral RNA detection from tissues was reduced with increasing antibody titer on day 20 PI. The results demonstrated that the IRFIBV32 virus has wide tissue distribution for respiratory, urogenital, and digestive systems.

Infectious Bronchitis Virus Associated with Nephropathy Lesions in Diagnostic Cases from Commercial Broiler Chickens in California

2020 ◽  
Vol 64 (4) ◽  
Author(s):  
Julia Blakey ◽  
Beate Crossley ◽  
Ana Da Silva ◽  
Daniel Rejmanek ◽  
Carmen Jerry ◽  
...  
Keyword(s):  
Infectious Bronchitis Virus ◽  
Broiler Chickens ◽  
Infectious Bronchitis ◽  
Commercial Broiler

scholarly journals First Complete Genome Sequence of Currently Circulating Infectious Bronchitis Virus Strain DMV/1639 of the GI-17 Lineage

2019 ◽  
Vol 8 (34) ◽  
Author(s):  
Iryna V. Goraichuk ◽  
Arun B. Kulkarni ◽  
Dawn Williams-Coplin ◽  
David L. Suarez ◽  
Claudio L. Afonso
Keyword(s):  
Genome Sequence ◽  
Virus Strain ◽  
Infectious Bronchitis Virus ◽  
Complete Genome Sequence ◽  
Complete Genome ◽  
Broiler Chickens ◽  
Economic Losses ◽  
Avian Infectious Bronchitis Virus ◽  
Infectious Bronchitis Virus Strain ◽  
Infectious Bronchitis

Avian infectious bronchitis virus is the causative agent of a highly contagious disease that results in severe economic losses to the poultry industry worldwide. Here, we report the first coding-complete genome sequence of strain DMV/1639 of the GI-17 lineage, isolated from broiler chickens in Georgia in 2019.

Comparative Evaluation of In Vitro and In Vivo Assays for the Detection of Avian Infectious Bronchitis Virus as a Contaminant of Live Poultry Vaccines

1998 ◽  
Vol 26 (5) ◽  
pp. 629-634
Author(s):  
Emiliana Falcone ◽  
Edoardo Vignolo ◽  
Livia Di Trani ◽  
Simona Puzelli ◽  
Maria Tollis
Keyword(s):  
Infectious Bronchitis Virus ◽  
Serological Response ◽  
Avian Infectious Bronchitis Virus ◽  
Animal Testing ◽  
Rt Pcr ◽  
Pcr Assay ◽  
In Vivo Test ◽  
Infectious Bronchitis

A reverse transcriptase polymerase chain reaction (RT-PCR) assay specific for identifying avian infectious bronchitis virus (IBV) in poultry vaccines, and the serological response to IBV induced by the inoculation of chicks with a Newcastle disease vaccine spiked with the Massachusetts strain of IBV, were compared for their ability to detect IBV as a contaminant of avian vaccines. The sensitivity of the IBV-RT-PCR assay provided results which were at least equivalent to the biological effect produced by the inoculation of chicks, allowing this assay to be considered a valid alternative to animal testing in the quality control of avian immunologicals. This procedure can easily be adapted to detect a number of contaminants for which the in vivo test still represents the only available method of detection.

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