Establishment of Salvia castanea Diels f. tomentosa Stib. hairy root cultures and the promotion of tanshinone accumulation and gene expression with Ag+, methyl jasmonate, and yeast extract elicitation

PROTOPLASMA ◽  
2015 ◽  
Vol 253 (1) ◽  
pp. 87-100 ◽  
Author(s):  
Bo Li ◽  
Bangqing Wang ◽  
Hongyan Li ◽  
Liang Peng ◽  
Mei Ru ◽  
...  
Keyword(s):  
Gene Expression ◽  
Methyl Jasmonate ◽  
Hairy Root ◽  
Yeast Extract ◽  
Hairy Root Cultures ◽  
Root Cultures

scholarly journals Enhanced production of valtrate in hairy root cultures of Valeriana jatamansi Jones by methyl jasmonate, jasmonic acid and salicylic acid elicitors

2020 ◽  
Vol 48 (2) ◽  
pp. 839-848
Author(s):  
Shuang ZHAO ◽  
Hong TANG
Keyword(s):  
Salicylic Acid ◽  
Jasmonic Acid ◽  
Methyl Jasmonate ◽  
Hairy Root ◽  
Hairy Roots ◽  
Hairy Root Cultures ◽  
Root Cultures ◽  
Potent Inducer ◽  
Valeriana Jatamansi ◽  
Pharmacologically Active

Valtrate is a pharmacologically active epoxyiridoid ester found in the roots and rhizomes of Valeriana jatamansi Jones. The plant produces only small amounts of this metabolite naturally, and so induction of hairy roots as well as elicitation can be useful to increase its commercial production. In this study, strain R1601 of Agrobacterium rhizogenes was used to induce hairy roots in V. jatamansi, and stable hairy root cultures of V. jatamansi were established successfully. The influence of three exogenous elicitors including methyl jasmonate (MJ), jasmonic acid (JA) and salicylic acid (SA) on valtrate production in the hairy root cultures of V. jatamansi was also investigated, and the 25-day-old hairy root cultures were treated with different concentrations of the elicitors at exposure time of 7 days. This present study showed that MJ (100 mg/L) highly promoted valtrate production at 7 days after elicitation, to a level of 3.63 times higher than that of non-elicited control. SA did not significantly increase the production of valtrate. This is the first-time study to assess the elicitation of hairy root cultures to promote valtrate biosynthesis in V. jatamansi and the resulting experiments demonstrated that MJ was indeed a potent inducer of valtrate biosynthesis.

scholarly journals INFLUENCE METHYL JASMONATE ON PRODUCTION OF ECDYSTEROIDS FROM HAIRY ROOTS OF SILENE LINICOLA C.C.GMELIN

2018 ◽  
pp. 159-167
Author(s):  
Анна (Anna) Алексеевна (Аlekseevna) Эрст (Erst) ◽  
Лариса (Larisa) Николаевна (Nikolayevna) Зибарева (Zibareva) ◽  
Елена (Elena) Сергеевна (Sergeyevna) Филоненко (Filonenko) ◽  
Татьяна (Tat'yana) Витальевна (Vital'yevna) Железниченко (Zheleznichenko)
Keyword(s):  
Methyl Jasmonate ◽  
Hairy Root ◽  
High Growth ◽  
Transformation Method ◽  
High Growth Rate ◽  
Hairy Root Cultures ◽  
Root Cultures ◽  
Experimental Conditions ◽  
Ecdysteroid Biosynthesis ◽  
Free Media

Hairy root cultures are being considered as promising system for producing valuable second metabolites. These genetically transformed root cultures are characterized by high growth rate, genetic stability and growth in hormone free media. Using A. rhizogenes-mediated transformation method (strain А-4), we have obtained hairy root cultures of the ecdysteroid-containing species of Silene linicola. HPLC analysis of the sample studied revealed that 20-hydroxyecdysone (20E), turkesterone and polypodin B were synthesized under the specified experimental conditions. Application of methyl jasmonate at a concentration of 100 μM resulted in stimulation of 20E biosynthesis (up 74%) after 3 days of cultivation and turkesterone up to 35% at 6 days.  It was noted that total ecdysteroid content in sample tested varied: turkesterone from 25 to 60%, and 20E from 8 to 30%. At the same time the level of 20E biosynthesis decreased from 0.023 to 0.014% in the samples without methyl jasmonate treatment. Hairy root lines of S. linicola with different responses to the presence of elicitors in the culture medium can be used to study the pathways of ecdysteroid biosynthesis.

scholarly journals Effects of methyl jasmonate on the growth and triterpenoid production of diploid and tetraploid Centella asiatica (L.) Urb. hairy root cultures

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Khoa Van Nguyen ◽  
Benyakan Pongkitwitoon ◽  
Thanika Pathomwichaiwat ◽  
Unchera Viboonjun ◽  
Sompop Prathanturarug
Keyword(s):  
Methyl Jasmonate ◽  
Hairy Root ◽  
Hairy Roots ◽  
Centella Asiatica ◽  
Root Culture ◽  
Hairy Root Culture ◽  
Asiatic Acid ◽  
Hairy Root Cultures ◽  
Root Cultures ◽  
Meja Treatment

AbstractIn this study, the effects of methyl jasmonate (MeJA) on the phytomass and triterpenoid production of diploid and tetraploid Centella asiatica hairy roots were investigated. Hairy root cultures were obtained from diploid and induced tetraploid plants of C. asiatica infected by Agrobacterium rhizogenes strain ATCC 43057. MeJA triggered triterpenoid production in both ploidy hairy roots, whereas triterpenoids were not produced in the untreated hairy roots. Among the treatments, the 50 µM MeJA treatment yielded the maximum triterpenoid production in diploid hairy roots of 27.25 ± 0.27 µg/mg Dry weight (DW) total triterpenoid at day 21. For the tetraploid hairy root cultures, the 28th-day hairy root culture produced a maximum amount of triterpenoids of 16.29 ± 6.32 µg/mg DW in response to the 50 µM MeJA treatment, whereas the 100 µM MeJA treatment produced a similar triterpenoid amount (16.31 ± 9.24 µg/mg DW) at day 14. Moreover, in response to 50 µM MeJA, we obtained different ratios of aglycone to glycoside, i.e., 1:7 and 1:2, between the diploid and tetraploid hairy root cultures. Asiaticoside was the dominant phytochemical, followed by asiatic acid and madecassic acid. This study provides valuable information for producing triterpenoids for C. asiatica commercial products and preparations by using hairy root cultures.

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