scholarly journals The expression of miRNA encoded by C19MC and miR-371-3 strongly varies among individual placentas but does not differ between spontaneous and induced abortions

PROTOPLASMA ◽  
2020 ◽  
Vol 258 (1) ◽  
pp. 209-218 ◽  
Author(s):  
Andrea Gottlieb ◽  
Inga Flor ◽  
Rolf Nimzyk ◽  
Lars Burchardt ◽  
Burkhard Helmke ◽  
...  
Keyword(s):  
Gestational Age ◽  
Mirna Gene ◽  
Placental Tissue ◽  
First Trimester ◽  
Maternal Serum ◽  
Interindividual Variation ◽  
Serum Samples ◽  
Trimester Abortion ◽  
Interindividual Differences ◽  
Induced Abortions

AbstractmiRNAs of the largest human miRNA gene cluster at all, i.e., C19MC, are almost exclusively expressed in the placenta. Nevertheless, only little is known about the interindividual variation of their expression and even about possible influence of gestational age, conflicting data is reported as well as for miRNAs of the much smaller miR-371-3 cluster. Our present study aims at the analyses of the expression of miRNAs from both clusters at different times of pregnancy, possible differences between placenta samples obtained from spontaneous or induced abortions in the first trimester, and the possible variation of miRNA expression at different sites within same placentas. miR-371a-3p, miR-372-3p, miR-373-3p, miR-517a-3p, and miR-520c-3p were quantified in 85 samples and miR-371a-3p was quantified in maternal serum samples taken immediately before delivery. While for miRNA-517a-3p and miR-520c-3p the expression increased with increasing gestational age, the present study revealed strong interindividual differences in the expression of miR-371-3 in full-term placental tissue as well as for miRNAs of the C19MC cluster, where the levels differed to a much lesser extent than for the former microRNAs. Also, strong interindividual differences were noted between the serum samples but differences related to the site of the placenta where the sample has been taken from were excluded. For neither of the data from placental tissue, the study revealed differences between the spontaneous and induced abortion group. Thus, the differences do not in general seem to be related to first trimester abortion. It remains to be elucidated whether or not they affect other prenatal processes.

scholarly journals First Trimester Maternal Cortisol Signatures in Small-for-gestational Age

2021 ◽  
Author(s):  
Chaelin Lee ◽  
Seung Mi Lee ◽  
Dong Jun Byun ◽  
So Yeon Kim ◽  
Hugh I. Kim ◽  
...  
Keyword(s):  
Pregnant Women ◽  
Gestational Age ◽  
Small For Gestational Age ◽  
Serum Levels ◽  
First Trimester ◽  
Maternal Serum ◽  
Serum Samples ◽  
Cortisol Metabolism ◽  
Cortisol Ratio ◽  
Pituitary Adrenal Axis

Abstract Background: Abnormal maternal hypothalamus-pituitary-adrenal axis is associated with fetal growth, and we hypothesized that the alteration in metabolic signatures of cortisol might be detectable during early pregnancy. The objective of this study was to identify predictable maternal serum signatures of cortisol metabolism during the first trimester of women who are expected to deliver small-for-gestational age (SGA) neonates.Methods: This prospective cohort study included 112 pregnant women (with and without SGA, n = 56 each). Maternal serum samples were collected at 10~14 gestational weeks to quantify the levels of cortisol and its precursors and metabolites by liquid chromatography-mass spectrometry.Results: Increased maternal serum levels of tetrahydrocortisol (THF, 11.82 ± 8.16 ng/mL vs. 7.51 ± 2.90 ng/mL, P < 0.005) and decreased 21-deoxycortisol (21-deoxyF, 2.98 ± 1.36 ng/mL vs. 4.33 ± 2.06 ng/mL, P < 0.0001) were observed in pregnant women carrying SGA fetus. In conjunction with individual steroid levels, metabolic ratios corresponding to the activity of related enzymes were calculated. In addition to increased THF/cortisol ratio (P < 0.006), the SGA group showed a significant increase in the two metabolic ratios including cortisol/11-deoxycortisol (F/11-deoxyF; P < 0.03) and cortisol/21-deoxycortisol (F/21-deoxyF; P < 0.0003) indicating cortisol biosynthesis. The ROC curve generated in combination with three variables of 21-deoxyF concentration and two metabolic ratios of F/21-deoxyF and THF/F resulted in AUC = 0.824 (95% confidence interval, 0.713 ~ 0.918). Conclusions: A significant decrease in maternal serum levels of 21-deoxyF and an increase in two metabolic ratios of F/21-deoxyF and THF/F, indicating cortisol biosynthetic rate, represent a reliable biomarker for the prediction of SGA in the first trimester.

scholarly journals Serum Concentrations of Selected Organochlorines in Pregnant Women and Associations with Pregnancy Outcomes. A Cross-Sectional Study from Two Rural Settings in Cambodia

2020 ◽  
Vol 17 (20) ◽  
pp. 7652
Author(s):  
Margit Steinholt ◽  
Shanshan Xu ◽  
Sam Ol Ha ◽  
Duong Trong Phi ◽  
Maria Lisa Odland ◽  
...  
Keyword(s):  
Polychlorinated Biphenyls ◽  
Pregnant Women ◽  
Gestational Age ◽  
Low Income ◽  
Cross Sectional Study ◽  
Maternal Serum ◽  
Sectional Study ◽  
Serum Samples ◽  
Cross Sectional ◽  
Rural Settings

We conducted a cross-sectional study among 194 pregnant women from two low-income settings in Cambodia. The inclusion period lasted from October 2015 through December 2017. Maternal serum samples were analyzed for persistent organic pollutants (POPs). The aim was to study potential effects on birth outcomes. We found low levels of polychlorinated biphenyls (PCBs) and organochlorine pesticides (OCP), except for heptachlors, β-hexachlorocyclohexane (HCH), heptachlor epoxide, and p,p’-DDE. There were few differences between the two study locations. However, the women from the poorest areas had significantly higher concentrations of p,p’-DDE (p < 0.001) and hexachlorobenzene (HCB) (p = 0.002). The maternal factors associated with exposure were parity, age, residential area, and educational level. Despite low maternal levels of polychlorinated biphenyls, we found significant negative associations between the PCB congeners 99 (95% CI: −2.51 to −0.07), 138 (95% CI: −1.28 to −0.32), and 153 (95% CI: −1.06 to −0.05) and gestational age. Further, there were significant negative associations between gestational age, birth length, and maternal levels of o,p’-DDE. Moreover, o,p’-DDD had positive associations with birth weight, and both p,p’-DDD and o,p’-DDE were positively associated with the baby’s ponderal index. The poorest population had higher exposure and less favorable outcomes.

scholarly journals M20. CYTOKINE LEVELS THROUGHOUT PREGNANCY AND RISK FOR PSYCHOSIS IN ADULT OFFSPRING: EARLY PREGNANCY MATTERS

2020 ◽  
Vol 46 (Supplement_1) ◽  
pp. S141-S141
Author(s):  
Dana Allswede ◽  
Robert Yolken ◽  
Stephen Buka ◽  
Tyrone Cannon
Keyword(s):  
Psychotic Disorder ◽  
Early Pregnancy ◽  
Serum Levels ◽  
First Trimester ◽  
Underlying Mechanism ◽  
Maternal Serum ◽  
Serum Samples ◽  
Birth Complications ◽  
Cytokine Levels ◽  
Pregnancy And Birth

Abstract Background Schizophrenia has been associated with pregnancy and birth complications, and fetal exposure to inflammation is thought to be a common underlying mechanism. However, it is unclear whether the risk associated with inflammation is specific to particular phases of pregnancy, as no prior studies have examined maternal serum samples across multiple assessments from the first trimester onward. This study examined differences in longitudinal patterns of maternal serum levels of TNFa, IL-1b, IL-5, IL-6, IL-8, IL-10, and IL-17a across pregnancy for offspring who were later ascertained as having a psychotic disorder diagnosis, non-psychotic siblings of these cases, and unrelated, non-psychotic individuals who served as controls. Methods Participants included 90 offspring, 79 siblings, and 273 matched controls from the Philadelphia cohort of the National Collaborative Perinatal Project. Psychotic disorder diagnoses in adulthood were assessed with review of medical records and were confirmed with a validation study. Cytokine levels were assessed using a multiplex bead assay in archived maternal serum samples collected across prenatal visits and birth. Results Levels of pro-inflammatory TNFa, IL-1b, and IL-6 were significantly higher in maternal serum of offspring who later developed psychosis relative to maternal serum of non-psychotic siblings and matched controls. These differences were maximal in first half of pregnancy (7–20 weeks), tapering to non-significant during the second half of pregnancy. Discussion These findings elucidate the importance of exposure to elevated maternal pro-inflammatory cytokine levels in early pregnancy to the etiology of psychosis.

Maternal serum CA 125 levels in first trimester abortion

1995 ◽  
Vol 60 (1) ◽  
pp. 35-36 ◽  
Author(s):  
Ivo Noci ◽  
Roberto Biagiotti ◽  
Enrico Periti ◽  
Daniele Baronci ◽  
Francesca Torricelli ◽  
...  
Keyword(s):  
First Trimester ◽  
Maternal Serum ◽  
Ca 125 ◽  
Trimester Abortion

First trimester maternal serum PAPP-A, β-hCG and ADAM12 in prediction of small-for-gestational-age fetuses

2008 ◽  
Vol 28 (12) ◽  
pp. 1131-1135 ◽  
Author(s):  
Kasper Pihl ◽  
Torben Larsen ◽  
Lone Krebs ◽  
Michael Christiansen
Keyword(s):  
Gestational Age ◽  
Small For Gestational Age ◽  
First Trimester ◽  
Maternal Serum ◽  
Β Hcg

OP37.01: First trimester maternal serum PP13 as screening test for preeclampsia and small gestational age

2010 ◽  
Vol 36 (S1) ◽  
pp. 158-158
Author(s):  
M. Parra-Cordero ◽  
R. Rodrigo ◽  
P. Barja ◽  
C. Bosco ◽  
R. Terra ◽  
...  
Keyword(s):  
Gestational Age ◽  
Screening Test ◽  
First Trimester ◽  
Maternal Serum

scholarly journals Maternal serum levels of perfluoroalkyl substances in early pregnancy and offspring birth weight

2019 ◽  
Vol 87 (6) ◽  
pp. 1093-1099 ◽  
Author(s):  
Sverre Wikström ◽  
Ping-I Lin ◽  
Christian H. Lindh ◽  
Huan Shu ◽  
Carl-Gustaf Bornehag
Keyword(s):  
Birth Weight ◽  
Prenatal Exposure ◽  
Gestational Age ◽  
Placental Transfer ◽  
Serum Levels ◽  
First Trimester ◽  
Maternal Serum ◽  
Perfluoroalkyl Substances ◽  
Perfluorodecanoic Acid ◽  
Mother And Child

Abstract Background Perfluoroalkyl substances (PFASs) are widespread, bioaccumulating, and persistent and show placental transfer. Emerging research indicates associations between prenatal exposure and low birth weight. The aim of this study was to assess the associations between first trimester exposure to PFASs and birth weight (BW) in the Swedish Environmental, Longitudinal, Mother and child, Asthma and allergy (SELMA) study and examine whether associations differ between girls and boys. Methods Eight PFASs were analyzed in maternal serum (median: 10 weeks of pregnancy). Associations between prenatal PFAS exposure and birth outcomes with BW, BW for gestational age, and birth small for gestational age (SGA) were assessed in 1533 infants, adjusted for potential confounders and stratified by sex. Results Increased maternal perfluorooctane sulfonate (PFOS), perfluorooctanoic acid (PFOA), perfluorononanoic acid (PFNA), perfluorodecanoic acid (PFDA), and perfluoroundecanoic acid (PFUnDA) were associated with lower BW, lower BW for gestational age, and SGA birth. Associations were significant only in girls, where prenatal exposure in the upper quartile was associated with a 93–142-g lower BW when compared with that of the lowest quartile exposure. The associations were not mediated by effects on gestational age. Conclusions We found associations between prenatal exposure for five different PFASs and birth weight, with more pronounced associations in girls than in boys.

Characterization of the role of microRNA-517a expression in low birth weight infants

2013 ◽  
Vol 4 (6) ◽  
pp. 522-526 ◽  
Author(s):  
G. Y. Song ◽  
W. W. Song ◽  
Y. Han ◽  
D. Wang ◽  
Q. Na
Keyword(s):  
Birth Weight ◽  
Low Birth Weight ◽  
Placental Tissue ◽  
Maternal Serum ◽  
Trophoblast Invasion ◽  
Normal Birth Weight ◽  
Serum Samples ◽  
Low Birth Weight Infants ◽  
Human Trophoblast ◽  
Normal Birth

The purpose of this study was to analyze the expression of the placenta-specific microRNA miR-517a in maternal serum and in placental tissue from low birth weight newborns and try to detect the effects of miR-517a expression on invasion potential of trophoblasts. Placental tissue and maternal serum were collected from both low birth weight newborns (n = 10) and normal birth weight newborns (n = 20). Expression of miR-517a was assessed in placenta and serum samples by real-time qRT-PCR. In addition, human trophoblast HTR8/SVneo cells were transfected with a miR-517a 2′-O-methyl oligonucleotide or a negative control RNA, and invasion was measured using transwell migration assays. Expression of miR-517a was significantly increased in placentas from low birth weight newborns (61.79 ± 23.06) in comparison with those of normal birth weight newborns (5.01 ± 1.97; P < 0.05). The expression of miR-517a was also increased in maternal serum isolated from the low birth weight newborn (25.78 ± 8.69) compared with the normal birth weight newborn (3.21 ± 1.07; P < 0.05). Overexpression of miR-517a significantly inhibited invasion of HTR8/SVneo cells (P < 0.05). These data indicate that miR-517a overexpression could potentially lead to low birth weight, likely through the inhibition of trophoblast invasion.

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