Statin stimulates bone morphogenetic protein-2, aggrecan, and type 2 collagen gene expression and proteoglycan synthesis in rat chondrocytes

2003 ◽  
Vol 8 (6) ◽  
pp. 842-848 ◽  
Author(s):  
Hiroshi Hatano ◽  
Akihiro Maruo ◽  
Mark E. Bolander ◽  
Gobinda Sarkar
Keyword(s):  
Gene Expression ◽  
Bone Morphogenetic Protein ◽  
Bone Morphogenetic Protein 2 ◽  
Proteoglycan Synthesis ◽  
Collagen Gene ◽  
Collagen Gene Expression ◽  
Morphogenetic Protein

scholarly journals Bone Morphogenetic Protein Signaling Is Required for Maintenance of Differentiated Phenotype, Control of Proliferation, and Hypertrophy in Chondrocytes

1998 ◽  
Vol 140 (2) ◽  
pp. 409-418 ◽  
Author(s):  
Motomi Enomoto-Iwamoto ◽  
Masahiro Iwamoto ◽  
Yoshiki Mukudai ◽  
Yasuhiko Kawakami ◽  
Tsutomu Nohno ◽  
...  
Keyword(s):  
Gene Expression ◽  
Alkaline Phosphatase ◽  
Bone Morphogenetic Protein ◽  
Bmp Signaling ◽  
Collagen Gene ◽  
Type Ii ◽  
Type X Collagen ◽  
Bmp Receptors ◽  
Collagen Gene Expression ◽  
Morphogenetic Protein

To examine the role of bone morphogenetic protein (BMP) signaling in chondrocytes during endochondral ossification, the dominant negative (DN) forms of BMP receptors were introduced into immature and mature chondrocytes isolated from lower and upper portions of chick embryo sternum, respectively. We found that control sternal chondrocyte populations expressed type IA, IB, and II BMP receptors as well as BMP-4 and -7. Expression of a DN-type II BMP receptor (termed DN-BMPR-II) in immature lower sternal (LS) chondrocytes led to a loss of differentiated functions; compared with control cells, the DN-BMPR- II–expressing LS chondrocytes proliferated more rapidly, acquired a fibroblastic morphology, showed little expression of type II collagen and aggrecan genes, and upregulated type I collagen gene expression. Expression of DN-BMPR-II in mature hypertrophic upper sternal (US) chondrocytes caused similar effects. In addition, the DN-BMPR-II–expressing US cells exhibited little alkaline phosphatase activity and type X collagen gene expression, while the control US cells produced both alkaline phosphatase and type X collagen. Both DN-BMPR-II–expressing US and LS chondrocytes failed to respond to treatment with BMP-2 . When we examined the effects of DN forms of types IA and IB BMP receptors, we found that DN-BMPR-IA had little effect, while DN-BMPR-IB had similar but weaker effects compared with those of DN-BMPR-II. We conclude that BMP signaling, particularly that mediated by the type II BMP receptor, is required for maintenance of the differentiated phenotype, control of cell proliferation, and expression of hypertrophic phenotype.

Gene Expression During Autograft Lumbar Spine Fusion and the Effect of Bone Morphogenetic Protein 2

1998 ◽  
Vol 351 ◽  
pp. 252???265 ◽  
Author(s):  
Michael A. Morone ◽  
Scott D. Boden ◽  
Gregory Hair ◽  
George J. Martin ◽  
Michele Racine ◽  
...  
Keyword(s):  
Gene Expression ◽  
Lumbar Spine ◽  
Bone Morphogenetic Protein ◽  
Spine Fusion ◽  
Bone Morphogenetic Protein 2 ◽  
Lumbar Spine Fusion ◽  
Morphogenetic Protein

scholarly journals Unsaturated Fatty Acids Disrupt Smad Signaling in Gonadotrope Cells Leading to Inhibition of FSHβ Gene Expression

Endocrinology ◽  
2014 ◽  
Vol 155 (2) ◽  
pp. 592-604 ◽  
Author(s):  
Ghislaine Garrel ◽  
Violaine Simon ◽  
Chantal Denoyelle ◽  
Muhammad Ishaq ◽  
Claude Rouch ◽  
...  
Keyword(s):  
Gene Expression ◽  
Bone Morphogenetic Protein ◽  
Protein Phosphatase ◽  
Unsaturated Fatty Acids ◽  
Reproductive Function ◽  
Bone Morphogenetic Protein 2 ◽  
Pituitary Cells ◽  
Transcript Levels ◽  
Morphogenetic Protein ◽  
Stimulation Of

Reproductive function is highly dependent on nutritional input. We recently provided evidence that the unsaturated ω6 fatty acid (FA), linoleic acid (linoleic), interferes with transcription and secretion of the gonadotropin LH, highlighting the existence of a lipid sensing in pituitary gonadotropes. Here, we show, using a combination of in vivo and in vitro models, that linoleic differentially regulates Lhb and Fshb expression. Central exposure of rats to linoleic over 7 days was associated with increase of Lhb but not Fshb transcript levels. Consistently, exposure of rat pituitary cells or LβT2 cells to linoleic increased Lhb, whereas it dramatically decreased Fshb transcript levels without affecting its stability. This effect was also induced by ω9 and ω3-polyunsaturated FA but not by saturated palmitic acid. Analysis of the underlying mechanisms in LβT2 cells using small interfering RNA revealed that early growth response protein 1 mediates linoleic stimulation of Lhb expression. Furthermore, we demonstrated that linoleic counteracts activin and bone morphogenetic protein-2 stimulation of Fshb expression. Using Western blotting and Smad-responsive reporter gene assays, linoleic was shown to decrease basal Smad2/3 phosphorylation levels as well as activin- and bone morphogenetic protein-2-dependent activation of Smad, uncovering a new FA-sensitive signaling cascade. Finally, the protein phosphatase magnesium-dependent 1A was shown to mediate linoleic inhibition of basal Smad phosphorylation and Fshb expression, identifying protein phosphatase magnesium-dependent 1A as a new target of FA in gonadotropes. Altogether, this study provides a novel mechanism by which FAs target gene expression and underlines the relevant role of pituitary gonadotropes in mediating the effects of nutritional FA on reproductive function.

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