Role of patellar tendon on the population of muscle fiber types and the contractile properties of single glycerinated muscle fibers in quadriceps muscles of rats

1998 ◽  
Vol 3 (2) ◽  
pp. 111-119 ◽  
Author(s):  
Koji Kidokoro ◽  
Yutaka Sasao ◽  
Kuniaki Shimizu ◽  
Hiroto Fujiya ◽  
Katsumasa Yamashita-Goto
Keyword(s):  
Patellar Tendon ◽  
Muscle Fiber ◽  
Muscle Fibers ◽  
Contractile Properties ◽  
Muscle Fiber Types ◽  
Fiber Types ◽  
Glycerinated Muscle

scholarly journals Metabolic and morphometric profile of muscle fibers in chronic hemodialysis patients

2012 ◽  
Vol 112 (1) ◽  
pp. 72-78 ◽  
Author(s):  
Michael I. Lewis ◽  
Mario Fournier ◽  
Huiyuan Wang ◽  
Thomas W. Storer ◽  
Richard Casaburi ◽  
...  
Keyword(s):  
Muscle Fiber ◽  
Muscle Fibers ◽  
Exercise Intolerance ◽  
Maintenance Hemodialysis ◽  
Muscle Fiber Types ◽  
Vastus Lateralis Muscle ◽  
Type I ◽  
Fiber Types ◽  
Dense Matrix ◽  
Individual Fiber

Muscle weakness and effort intolerance are common in maintenance hemodialysis (MHD) patients. This study characterized morphometric, histochemical, and biochemical properties of limb muscle in MHD patients compared with controls (CTL) with similar age, gender, and ethnicity. Vastus lateralis muscle biopsies were obtained from 60 MHD patients, 1 day after dialysis, and from 21 CTL. Muscle fiber types and capillaries were identified immunohistochemically. Individual muscle fiber cross-sectional areas (CSA) were quantified. Individual fiber oxidative capacities were determined (microdensitometric assay) to measure succinate dehydrogenase (SDH) activity. Mean CSAs of type I, IIA, and IIX fibers were 33, 26, and 28% larger in MHD patients compared with CTL. SDH activities for type I, IIA, and IIX fibers were reduced by 29, 40, and 47%, respectively, in MHD. Capillary to fiber ratio was increased by 11% in MHD. The number of capillaries surrounding individual fiber types were also increased (type I: 9%; IIA: 10%; IIX: 23%) in MHD patients. However, capillary density (capillaries per unit muscle fiber area) was reduced by 34% in MHD patients, compared with CTL. Ultrastuctural analysis revealed swollen mitochondria with dense matrix in MHD patients. These results highlight impaired oxidative capacity and capillarity in MHD patients. This would be expected to impair energy production as well as substrate and oxygen delivery and exchange and contribute to exercise intolerance. The enlarged CSA of muscle fibers may, in part, be accounted for by edema. We speculate that these changes contribute to reduce limb strength in MHD patients by reducing specific force.

scholarly journals LncRNA-FKBP1C regulates muscle fiber type switching by affecting the stability of MYH1B

2021 ◽  
Vol 7 (1) ◽  
Author(s):  
Jia-ao Yu ◽  
Zhijun Wang ◽  
Xin Yang ◽  
Manting Ma ◽  
Zhenhui Li ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Protein Stability ◽  
Muscle Fiber ◽  
Fiber Type ◽  
Muscle Fibers ◽  
Fast Muscle ◽  
Muscle Fiber Types ◽  
Fiber Types ◽  
Regulatory Processes ◽  
Muscle Genes

AbstractLong non-coding RNAs (lncRNAs) are well-known to participate in a variety of important regulatory processes in myogenesis. In our previous RNA-seq study (accession number GSE58755), we found that lncRNA-FKBP1C was differentially expressed between White Recessive Rock (WRR) and Xinghua (XH) chicken. Here, we have further demonstrated that lncRNA-FKBP1C interacted directly with MYH1B by biotinylated RNA pull-down assay and RNA immunoprecipitation (RIP). Protein stability and degradation experiments identified that lncRNA-FKBP1C enhanced the protein stability of MYH1B. Overexpression of lncRNA-FKBP1C inhibited myoblasts proliferation, promoted myoblasts differentiation, and participated in the formation of skeletal muscle fibers. LncRNA-FKBP1C could downregulate the fast muscle genes and upregulate slow muscle genes. Conversely, its interference promoted cell proliferation, repressed cell differentiation, and drove the transformation of slow-twitch muscle fibers to fast-twitch muscle fibers. Similar results were observed after knockdown of the MYH1B gene, but the difference was that the MYH1B gene had no effects on fast muscle fibers. In short, these data demonstrate that lncRNA-FKBP1C could bound with MYH1B and enhance its protein stability, thus affecting proliferation, differentiation of myoblasts and conversion of skeletal muscle fiber types.

Long-term effects of clenbuterol on diaphragm morphology and contractile properties in emphysematous hamsters

1998 ◽  
Vol 85 (1) ◽  
pp. 215-222 ◽  
Author(s):  
H. F. M. Van Der Heijden ◽  
P. N. R Dekhuijzen ◽  
H. Folgering ◽  
L. A. Ginsel ◽  
C. L. A. Van Herwaarden
Keyword(s):  
Muscle Fiber ◽  
Contractile Properties ◽  
Diaphragm Muscle ◽  
Muscle Fiber Types ◽  
Type I ◽  
Fiber Types ◽  
Force Frequency ◽  
Long Term Effects ◽  
Muscle Weight

The aim of the present study was to investigate the effect of chronic long-term clenbuterol treatment (1 mg/kg subcutaneously twice a day for 12 wk) on diaphragm morphology and function in emphysematous (EH) and normal hamsters (NH). Clenbuterol increased body weight, diaphragm weight, and skeletal muscle weight in both EH and NH to a similar extent. In the diaphragm, clenbuterol significantly increased myosin heavy chain type I, IIa, and IIx muscle fiber cross-sectional areas by ∼35–55% in both EH and NH. This response to clenbuterol treatment was not significantly different between EH and NH diaphragm. In EH, twitch force (Pt), maximal tetanic force, and force-frequency curve were significantly reduced compared with NH. In EH, clenbuterol increased Pt by ∼10%, restoring Pt to NH level. A similar improvement was observed in the force-frequency characteristics. Clenbuterol did not alter contractile properties in NH. In conclusion, long-term clenbuterol treatment resulted in an increased size of all diaphragm muscle fiber types in both NH and EH. Clenbuterol completely abolished the reduced force generation induced by emphysema.

Distribution of muscle fiber types and EMG activity in cat intercostal muscles

1990 ◽  
Vol 69 (4) ◽  
pp. 1208-1211 ◽  
Author(s):  
J. J. Greer ◽  
T. P. Martin
Keyword(s):  
Muscle Fiber ◽  
Fiber Type ◽  
Muscle Fibers ◽  
Emg Activity ◽  
Clear Correlation ◽  
Muscle Fiber Types ◽  
Fiber Types ◽  
Respiratory Drive ◽  
Intercostal Muscles ◽  
Anesthetized Cat

The electromyogram (EMG) activity and histochemical properties of intercostal muscles in the anesthetized cat were studied. The parasternal muscles were consistently active during inspiration. The external intercostals in the rostral spaces and the ventral portions of the midthoracic spaces were also recruited during inspiration. The remaining external intercostals were typically silent, regardless of the level of respiratory drive. The internal intercostal muscles located in the caudal spaces were occasionally recruited during expiration. There was a clear correlation between recruitment patterns of the intercostals and the histochemically defined fiber type properties of the muscles. Intercostal muscles that were routinely recruited during inspiration had a significantly higher proportion of slow-oxidative muscle fibers.

Identification of separate slow and fast muscle precursor cells in vivo, prior to somite formation

Development ◽  
1996 ◽  
Vol 122 (11) ◽  
pp. 3371-3380 ◽  
Author(s):  
S.H. Devoto ◽  
E. Melancon ◽  
J.S. Eisen ◽  
M. Westerfield
Keyword(s):  
Muscle Fiber ◽  
Fluorescent Dyes ◽  
Muscle Fibers ◽  
Precursor Cells ◽  
Fast Muscle ◽  
Muscle Fiber Types ◽  
Fiber Types ◽  
Axial Muscle ◽  
Somite Formation ◽  
Adaxial Cells

We have examined the development of specific muscle fiber types in zebrafish axial muscle by labeling myogenic precursor cells with vital fluorescent dyes and following their subsequent differentiation and fate. Two populations of muscle precursors, medial and lateral, can be distinguished in the segmental plate by position, morphology and gene expression. The medial cells, known as adaxial cells, are large, cuboidal cells adjacent to the notochord that express myoD. Surprisingly, after somite formation, they migrate radially away from the notochord, becoming a superficial layer of muscle cells. A subset of adaxial cells develop into engrailed-expressing muscle pioneers. Adaxial cells differentiate into slow muscle fibers of the adult fish. We have named the lateral population of cells in the segmental plate, lateral presomitic cells. They are smaller, more irregularly shaped and separated from the notochord by adaxial cells; they do not express myoD until after somite formation. Lateral presomitic cells remain deep in the myotome and they differentiate into fast muscle fibers. Thus, slow and fast muscle fiber types in zebrafish axial muscle arise from distinct populations of cells in the segmental plate that develop in different cellular environments and display distinct behaviors.

Histology of Human Eustachian Tube Muscles: Effect of Aging

1984 ◽  
Vol 93 (1) ◽  
pp. 17-24 ◽  
Author(s):  
Koichi Tomoda ◽  
Sotokichi Morii ◽  
Toshio Yamashita ◽  
Tadami Kumazawa
Keyword(s):  
Eustachian Tube ◽  
Muscle Fiber ◽  
Muscle Fibers ◽  
Maximum Growth ◽  
Muscle Fiber Types ◽  
Fiber Types ◽  
Elderly Adults ◽  
The Third ◽  
Long Period ◽  
Main Component

Forty-eight specimens of human eustachian tube tensor and levator muscles were examined histologically in order to study their functions and the effect of aging. The muscle fibers reach their maximum growth in the third decade with a narrow short diameter: tensor 21.8 μm and levator 24.5 μm; with advancing age, they were prone to atrophy, particularly in the tensor. Three muscle fiber types, red, white, and intermediate, were identified in the tubal muscles. The white fibers predominated in the tensor, and they were a main component of muscle fibers in fetuses and elderly adults. Lipofuscins were located in the periphery of muscle fibers, dominantly in the levator, and became numerous and larger with age. Our observations suggest that 1) the tensor produces a rapid opening of the eustachian tube while the levator creates tension and dilates the pharyngeal orifice of the tube for a relatively long period, and 2) these muscle functions deteriorate with advancing age.

Physiological, morphological, and histochemical properties of cat external anal sphincter

1988 ◽  
Vol 255 (6) ◽  
pp. G772-G778 ◽  
Author(s):  
J. Krier ◽  
T. Adams ◽  
R. A. Meyer
Keyword(s):  
Muscle Fiber ◽  
Fiber Type ◽  
Muscle Fibers ◽  
Succinic Dehydrogenase ◽  
Muscle Fiber Types ◽  
Fiber Types ◽  
Motor Axons ◽  
Fast Twitch Muscle ◽  
Fast Twitch ◽  
Stimulation Of

The contractile properties, morphology, and the distribution of striated muscle fiber types of the external and sphincter (EAS) were determined using axial force measurements, fiber size cross-sectional area measurements, and histochemistry. Electrical stimulation of motor axons in pudendal nerve at supramaximal intensities (10 V, 0.05 ms duration) elicited twitch contractions of EAS. The time to peak force after a single pulse ranged from 37 to 42 ms. The time for relaxation to half-maximal twitch force ranged from 20 to 29 ms. Repetitive stimulation of motor axons (0.1-3.0 Hz) produced potentiation and fatigue of single twitch contractile force, suggesting that the EAS of the cat is comprised predominantly of fast-twitch muscle fibers. Confirmation of skeletal muscle fiber types was determined by histochemistry. Frozen serial cross sections of EAS were incubated to demonstrate succinic dehydrogenase (SDH) and myosin adenosine triphosphatase after alkaline preincubation (pH 10.4). Based on these reactions, muscle fibers were classified as fast glycolytic (FG) (high ATPase, low SDH), fast oxidative-glycolytic (FOG) (high ATPase, high SDH), and slow oxidative (SO) (low ATPase, high SDH). The mean percentage +/- SE of each histochemical type was the following: FG, 73.5 +/- 3.9; FOG, 22.8 +/- 3.7; and SO, 3.7 +/- 0.6. These results indicate that the predominant fiber type for the EAS is FG. The EAS of the cat is considered a nominally fast-twitch muscle.

scholarly journals Muscle Fiber Types in Crabs: Studies on Single Identified Muscle Fibers

1987 ◽  
Vol 27 (4) ◽  
pp. 1067-1077 ◽  
Author(s):  
WERNER RATHMAYER ◽  
LOTHAR MAIER
Keyword(s):  
Muscle Fiber ◽  
Muscle Fibers ◽  
Muscle Fiber Types ◽  
Fiber Types

Lateral rectus muscle changes after bilateral neonatal labyrinthectomy in the ferret

2005 ◽  
Vol 15 (5-6) ◽  
pp. 243-251
Author(s):  
Mary S. Shall ◽  
Susan Van Cleave ◽  
Diana M. Dimitrova ◽  
Stephen J. Goldberg
Keyword(s):  
Eye Movements ◽  
Inner Ear ◽  
Muscle Fiber ◽  
Vestibular System ◽  
Muscle Fibers ◽  
Rectus Muscle ◽  
Head Movements ◽  
Lateral Rectus ◽  
Muscle Fiber Types ◽  
Fiber Types

The vestibular system is essential to the coordination of eye movements during head movements. Exercise, such as the eye movements mediated by the vestibular system, is a major factor in the development of muscle fiber types and the strength of muscle. In this study, the contents of the inner ear were removed (labyrinthectomy) from (LAB) ferrets at postnatal day 10 (P10) and raised with control (SHAM) animals. At P30, the lateral rectus muscles (LR) were removed to analyze the expression of myosin heavy chain (MHC) isoforms and to measure the least diameter of the developmental, slow and fast type muscle fibers. Another set of animals were sacrificed at P120 to analyze MHC isoform expression and muscle fiber diameters, as well as the contractile characteristics of the LR were measured prior to sacrifice. The average LAB LR was significantly stronger than the SHAM LR and the muscle fibers of the LAB animals were larger in diameter. The LAB animals expressed more type IIx and less slow type MHC. These results support the hypothesis that input from the inner ear influence the development of strength and muscle diameter in the ferret extraocular muscles.

Simultaneous cytochemical demonstration of muscle fiber types and acetylcholinesterase in muscle fibers of dystrophic chickens☆

1978 ◽  
Vol 60 (1) ◽  
pp. 68-82 ◽  
Author(s):  
C.R. Ashmore ◽  
P. Vigneron ◽  
L. Marger ◽  
L. Doerr
Keyword(s):  
Muscle Fiber ◽  
Muscle Fibers ◽  
Muscle Fiber Types ◽  
Fiber Types ◽  
Cytochemical Demonstration
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