Effect of double antibiotic and calcium hydroxide pastes on dislodgement resistance of an epoxy resin-based and two calcium silicate-based root canal sealers

Objective.The present study assessed the chemical elements in two novel calcium silicate-containing root canal sealers, BioRoot RCS and Well-Root ST, compared to a calcium silicate-containing root canal sealer that has been on the market for several years, MTA Fillapex, and epoxy resin-based sealer AHPlus.Material and Methods.The sealers were mixed and manipulated according to the manufacturers’ instructions. Twelve cylindrical molds (inner diameter 4 mm; height 3 mm) were placed on a glass petri dish and packed with the materials. The dish was transferred to an incubator. After 72 h the molds were examined by scanning electron microscopy and energy dispersive X-ray microanalysis.Results.BioRoot RCS and Well-Root ST had high peaks of calcium, zirconium, oxygen, carbon, silicon, and chlorine. Well-Root ST also had sodium, magnesium, aluminum, and titanium peaks. MTA Fillapex and AHPlus had carbon, oxygen, calcium, titanium, and bismuth peaks. A silicon peak was also observed for MTA Fillapex, and zirconium and tungsten peaks for AHPlus.Conclusion.BioRoot RSC had the highest degree of purity. The clinical implication of metals contained in the other sealers needs to be investigated.

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An in vitro study on the effects of serum proteins on Enterococcus faecalis adhesion to three types of root sealers and gutta-percha

BMC Oral Health ◽

10.1186/s12903-021-01992-4 ◽

2021 ◽

Vol 21 (1) ◽

Author(s):

Xinwei Lin ◽

Danlu Chi ◽

Qimei Gong ◽

Zhongchun Tong

Keyword(s):

Epoxy Resin ◽

Calcium Hydroxide ◽

Enterococcus Faecalis ◽

Root Canal ◽

Serum Proteins ◽

Laser Scanning Microscopy ◽

Root Canal Sealer ◽

Gutta Percha ◽

Ah Plus ◽

Root Canal Sealers

Abstract Background The extrusion of overfilled materials that extend beyond the apical foramina into the periradicular tissue may serve as a reservoir for bacterial adhesion and further affect recovery from periapical diseases. The aim of this study was to evaluate the effects of serum proteins on Enterococcus faecalis adhesion and survival on the surface of a calcium hydroxide-based root canal sealer (Apexit Plus), an epoxy resin sealer (AH-Plus) and a bioceramic sealer (iRoot SP). Methods Apexit Plus, AH-Plus and iRoot SP were evenly coated on gutta-percha, using gutta-percha alone as the control. After root canal sealer setting, the number of E. faecalis adhering to the root canal sealers and gutta-percha was counted in fetal bovine serum (FBS) or tryptic soy broth supplemented with 1% glucose (TSBG) by viable cell plate counts. The morphology of 7-day-old E. faecalis biofilms in FSB and TSBG was observed by scanning electron microscopy (SEM). Furthermore, E. faecalis biofilms on the three root canal sealers were labeled with a LIVE/DEAD BacLight™ Bacterial Viability Kit, and the ratios of viable to dead cells were analyzed using laser scanning microscopy operative software (Zen software). Results In the assays, after 1 and 7 days, the number of E. faecalis adhering to the root canal sealers or gutta-percha in FBS were significantly lower than those in TSBG (P < 0.05). In FBS, E. faecalis adhesion to iRoot SP and gutta-percha was reduced to a greater extent than that adhered to Apexit Plus and AH-Plus. Few E. faecalis accumulated on iRoot SP in FBS, whereas many bacteria assembled on iRoot SP and formed biofilms in TSBG. The ratio of viable cells in the E. faecalis biofilm on iRoot SP was the lowest. Conclusions Calcium hydroxide-based root canal sealers, epoxy resin sealers and bioceramic sealers may provide a substrate for E. faecalis adhesion, and the bioceramic sealer in this study showed the least E. faecalis adhesion in the presence of serum proteins compared to the other two sealers.

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Evaluation of the Genotoxicity of Zinc Oxide Eugenol-Based, Calcium Hydroxide-Based, and Epoxy Resin-Based Root Canal Sealers by Comet Assay

Journal of Endodontics ◽

10.1097/00004770-200112000-00008 ◽

2001 ◽

Vol 27 (12) ◽

pp. 744-748 ◽

Cited By ~ 25

Author(s):

T HUANG ◽

D HUEILEE ◽

C KAO

Keyword(s):

Zinc Oxide ◽

Comet Assay ◽

Epoxy Resin ◽

Calcium Hydroxide ◽

Root Canal ◽

Zinc Oxide Eugenol ◽

Root Canal Sealers

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Antimicrobial activity of root canal sealers

Stomatoloski glasnik Srbije ◽

10.2298/sgs0602104v ◽

2006 ◽

Vol 53 (2) ◽

pp. 104-112 ◽

Cited By ~ 1

Author(s):

Mirjana Vujaskovic ◽

Branka Radosavljevic

Keyword(s):

Antimicrobial Activity ◽

Zinc Oxide ◽

Epoxy Resin ◽

Calcium Hydroxide ◽

Root Canal ◽

Antimicrobial Effect ◽

Inhibition Zone ◽

Glass Ionomer ◽

Zinc Oxide Eugenol ◽

Root Canal Sealers

All root canal sealers should have antimicrobial activity so that they could act against any remaining bacteria after root canal obturation. The aim of this study was to evaluate the antimicrobial activity of five root canal sealers: AH26, a resin based paste; Apexit, calcium hydroxide based paste; Endomethasone and Tubliseal, zinc oxide eugenol based materials and Ketac Endo Aplicap, glass ionomer based sealer. Matherials And Methods: Antimicrobial activity was tested against S. mutans 70C and L.casei ATCC 27773 using ADT (agar diffusion inhibitory test) on TYC SB, blood and MRS agars. Freshly prepared paste or cement was placed in grooves of 4 mm in diameter in agar plates. Prepared agar plates were incubated at 37?C in GAS PAC system for 24hours. The antimicrobial effect was determined according to the diameter of growth inhibition zone around tested materials. Three measurements were done for each material and values were recorded in millimeters. Statistical analysis was done using Mann Whitney test. The results confirmed that epoxy resin and zinc oxide eugenol based sealers had the greatest antimicrobial effect. Calcium hydroxide and glass ionomer based sealers showed significantly lower antimicrobial activity compared to AH26, Endomethasone and Tubliseal. Conclusion: The greatest antimicrobial activity was found for epoxy resin based sealer (AH26) for both tested microorganisms.

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