scholarly journals An in vitro study on the effects of serum proteins on Enterococcus faecalis adhesion to three types of root sealers and gutta-percha

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Xinwei Lin ◽  
Danlu Chi ◽  
Qimei Gong ◽  
Zhongchun Tong
Keyword(s):  
Epoxy Resin ◽  
Calcium Hydroxide ◽  
Enterococcus Faecalis ◽  
Root Canal ◽  
Serum Proteins ◽  
Laser Scanning Microscopy ◽  
Root Canal Sealer ◽  
Gutta Percha ◽  
Ah Plus ◽  
Root Canal Sealers

Abstract Background The extrusion of overfilled materials that extend beyond the apical foramina into the periradicular tissue may serve as a reservoir for bacterial adhesion and further affect recovery from periapical diseases. The aim of this study was to evaluate the effects of serum proteins on Enterococcus faecalis adhesion and survival on the surface of a calcium hydroxide-based root canal sealer (Apexit Plus), an epoxy resin sealer (AH-Plus) and a bioceramic sealer (iRoot SP). Methods Apexit Plus, AH-Plus and iRoot SP were evenly coated on gutta-percha, using gutta-percha alone as the control. After root canal sealer setting, the number of E. faecalis adhering to the root canal sealers and gutta-percha was counted in fetal bovine serum (FBS) or tryptic soy broth supplemented with 1% glucose (TSBG) by viable cell plate counts. The morphology of 7-day-old E. faecalis biofilms in FSB and TSBG was observed by scanning electron microscopy (SEM). Furthermore, E. faecalis biofilms on the three root canal sealers were labeled with a LIVE/DEAD BacLight™ Bacterial Viability Kit, and the ratios of viable to dead cells were analyzed using laser scanning microscopy operative software (Zen software). Results In the assays, after 1 and 7 days, the number of E. faecalis adhering to the root canal sealers or gutta-percha in FBS were significantly lower than those in TSBG (P < 0.05). In FBS, E. faecalis adhesion to iRoot SP and gutta-percha was reduced to a greater extent than that adhered to Apexit Plus and AH-Plus. Few E. faecalis accumulated on iRoot SP in FBS, whereas many bacteria assembled on iRoot SP and formed biofilms in TSBG. The ratio of viable cells in the E. faecalis biofilm on iRoot SP was the lowest. Conclusions Calcium hydroxide-based root canal sealers, epoxy resin sealers and bioceramic sealers may provide a substrate for E. faecalis adhesion, and the bioceramic sealer in this study showed the least E. faecalis adhesion in the presence of serum proteins compared to the other two sealers.

scholarly journals Antibacterial and antifungal activity of an MTA-Based root canal sealer versus epoxy resin-based and methacrylate resin-based sealers

2020 ◽  
Vol 10 (3) ◽  
pp. 66-72
Author(s):  
Ali Türkyılmaz ◽  
Ali Erdemir
Keyword(s):  
Antimicrobial Activity ◽  
Antifungal Activity ◽  
Epoxy Resin ◽  
Root Canal ◽  
Root Canal Sealer ◽  
Antibacterial And Antifungal Activity ◽  
Mueller Hinton ◽  
Ah Plus ◽  
Antibacterial And Antifungal ◽  
Root Canal Sealers

Aim: The purpose of this study was to compare the antibacterial and antifungal activity of MTA Fillapex with AH 26, AH Plus, and RealSeal root canal sealers. S. aureus, E. faecalis, and C. albicans were used as test microorganisms with the agar-diffusion test (ADT) and the direct contact test (DCT).   Methodology: For the ADT, 48 Mueller-Hinton plates were divided into 3 groups according to the microorganism used. Each group was then divided into 4 subgroups according to root canal sealer. Mueller-Hinton and Sabouraud agar mediums were preferred, and inhibition zones were measured to determine the antimicrobial efficacy at designated intervals. In the DCT, 96-well microtiter plates were used. For each microorganism and each sealer, 8 consecutive wells were prepared vertically on the plate. Microbial suspensions were allowed to directly contact the sealers in each well for 1 hour at 37°C. Subsequently, microbial growth was spectrophotometrically measured at set intervals for the freshly mixed and set forms. Results: A statistically significant difference was found between the tested root canal sealers for antimicrobial effectiveness (p < 0.05). According to the ADT results, all sealers had antimicrobial activity against the tested microorganisms. MTA Fillapex demonstrated satisfying results in the ADT against all microorganisms. In the DCT, MTA Fillapex inhibited bacterial and fungal growth in all freshly mixed and set forms. However, the set forms of AH 26 and AH Plus began to lose their antimicrobial activity on the tested microorganisms after a while. Conclusion: The results showed that the MTA-based root canal sealer MTA Fillapex may be a favorable alternative sealer against bacterial and/or fungal species in clinical practice.   How to cite this article: Türkyılmaz A, Erdemir A. Antibacterial and antifungal activity of MTA-based root canal sealer versus epoxy resin-based and methacrylate resin-based sealers. Int Dent Res 2020;10(3):66-72.  https://doi.org/10.5577/intdentres.2020.vol10.no3.1   Linguistic Revision: The English in this manuscript has been checked by at least two professional editors, both native speakers of English.

scholarly journals Evaluation of apical microleakage of teeth sealed with four different root canal sealers

2006 ◽  
Vol 14 (5) ◽  
pp. 341-345 ◽  
Author(s):  
Fábio Dultra ◽  
Juliana Machado Barroso ◽  
Laise Daniela Carrasco ◽  
Alexandre Capelli ◽  
Danilo M. Zanello Guerisoli ◽  
...  
Keyword(s):  
Root Canal ◽  
Methyl Salicylate ◽  
The Other ◽  
Root Canal Sealer ◽  
Root Canals ◽  
Dye Penetration ◽  
Gutta Percha ◽  
India Ink ◽  
Ah Plus ◽  
Root Canal Sealers

OBJECTIVES: to compare the apical sealing ability of four root canal sealers. MATERIAL AND METHODS: forty extracted human maxillary canines were instrumented 1 mm short of the anatomical apex and randomly assigned to four groups (n=10), according to the root canal sealer used for obturation: Endofill, AH Plus, EndoREZ and Epiphany. Root canals were obturated with gutta-percha points, except for the Epiphany group, in which resin points (Resilon) were used. The teeth were immersed in India ink for seven days and clarified using methyl salicylate. The extent of apical dye penetration was measured with a measuroscope in all aspects of the canal. RESULTS: AH Plus (0.02 mm ± 0.07), Epiphany (0.00 mm ± 0.00) and EndoREZ (0.32 mm ± 0.62) did not differ statistically to each other (p>0.01). EndoFill presented the highest dye penetration mean (0.83 mm ± 0.73) and was statistically different from the other sealers (p<0.01). CONCLUSION: the resin-based root canal sealers presented lesser apical microleakage than the zinc oxide and eugenol based sealer. No statistical differences were observed among resin based sealers.

scholarly journals Evaluation of the radiopacity of root canal sealers by digitization of radiographic images

2004 ◽  
Vol 12 (4) ◽  
pp. 355-357 ◽  
Author(s):  
Juliane Maria Guerreiro Tanomaru ◽  
Luciana Cezare ◽  
Marcelo Gonçalves ◽  
Mário Tanomaru Filho
Keyword(s):  
Zinc Oxide ◽  
Calcium Hydroxide ◽  
Root Canal ◽  
The Other ◽  
Root Canal Sealer ◽  
Radiographic Images ◽  
Ah Plus ◽  
Root Canal Sealers

The aim of this study was to evaluate the radiopacity of a zinc oxide and eugenol-based (Endofill), a calcium hydroxide-based (Sealapex), two resin-based (Sealer 26 and AH Plus), and a silicone-based root canal sealer (Roeko Seal). Specimens, measuring 10mm in diameter and 1mm in thickness, were radiographed simultaneously with an aluminum step wedge using occlusal films, according to ISO 6876/2001 standards. Radiographs were digitized, and the radiopacity of sealers was compared to the different thicknesses of the aluminum step wedge, using the VIXWIN 2000 software. Results demonstrated that AH Plus was the most radiopaque sealer, while Sealapex was the least radiopaque (p<0.05). Roeko Seal, Endofill and Sealer 26 presented intermediate radiopacity values. Sealapex presented less radiopacity than the other types of root canal sealers.

scholarly journals The Penetration Ability of Calcium Silicate Root Canal Sealers into Dentinal Tubules Compared to Conventional Resin-Based Sealer: A Confocal Laser Scanning Microscopy Study

Materials ◽  
2019 ◽  
Vol 12 (3) ◽  
pp. 531 ◽  
Author(s):  
Yemi Kim ◽  
Ban-Suk Kim ◽  
Yong-Min Kim ◽  
Donghee Lee ◽  
Sin-Young Kim
Keyword(s):  
Root Canal ◽  
Laser Scanning ◽  
Laser Scanning Microscopy ◽  
Confocal Laser ◽  
Intensity Level ◽  
Single Cone ◽  
Penetration Ability ◽  
Ah Plus ◽  
Root Canal Sealers ◽  
Apical Area

The purpose of this study was to compare the penetration ability of calcium silicate root canal sealers and conventional resin-based sealer using confocal laser scanning microscopy (CLSM). A total of 60 recently extracted single-rooted human premolars were used in this study. The root canals were prepared to a size 40/0.06 taper with ProFile rotary instruments and irrigated with NaOCl and EDTA. After drying all canals, the specimens were randomly divided into three experimental groups (n = 20): Group 1, gutta-percha (GP)/AH Plus with continuous wave compaction; group 2, GP/BioRoot RCS with a single-cone technique; and group 3, GP/Endoseal MTA with a single-cone technique. All experimental samples were sectioned perpendicular to their long axis using a low-speed diamond wheel at the apical, middle, and coronal third levels. The penetration abilities of all samples were evaluated using CLSM. A Kruskal–Wallis analysis and a series of Mann–Whitney U post hoc tests were performed. A higher intensity level was found in the coronal area and a lower intensity level in the apical area in all the experimental groups. The AH Plus group showed higher sum fluorescence intensity in the apical and coronal thirds compared with the BioRoot RCS and Endoseal MTA groups, whereas the BioRoot RCS group showed a higher intensity level in the middle third, similar to the AH Plus group. The maximum sealer penetration depth was low in the apical area and high in the coronal area in the AH Plus and Endoseal MTA groups. In the BioRoot RCS group, maximum sealer penetration was observed in the middle third. In conclusion, there were significant differences in sealer penetration pattern and distance according to the root level and sealer type.

Staining Potential of Calcium Hydroxide and Monochlorophenol Following Removal of AH26 Root Canal Sealer

2008 ◽  
Vol 9 (3) ◽  
pp. 56-63 ◽  
Author(s):  
Ali Cemal Tinaz ◽  
Baǧdagül Helvaciölu Kivanç ◽  
Güliz Gürgül
Keyword(s):  
Calcium Hydroxide ◽  
Root Canal ◽  
Filling Material ◽  
Root Canal Sealer ◽  
Tooth Structure ◽  
Root Canals ◽  
Gutta Percha ◽  
Anterior Teeth ◽  
Tooth Surfaces ◽  
Tooth Preparation

Abstract Aim The focus of this study was to examine the staining potential of calcium hydroxide (Ca(OH)2) on tooth structure following the removal of AH26 root canal sealer. Methods and Materials Fifty maxillary anterior teeth were prepared and obturated with AH26 and gutta percha. The sealers were then removed 24 hours later and the teeth were randomly divided into two groups. Ca(OH)2 was then placed in the root canals of the first group of teeth as a medicament and camphorated monochlorophenol (CMCP) was placed in the second group of teeth after the filling material was removed. The color of the external tooth surfaces was determined before tooth preparation and two weeks after the placement of the medicaments. The Z test was used for statistical analysis. Results All experimental teeth showed varying degrees of coronal discoloration with the Ca(OH)2 group showing more discoloration than the CMCP group (p<0.05). Conclusion Using Ca(OH)2 as a medicament after removing AH26 caused progressive discoloration of the teeth, whereas using CMCP caused only slight discoloration. Clinical Significance To avoid staining of the treated tooth, AH26 root canal sealer must be completely removed from the dentin walls before using a medicament. Citation Tinaz AC, Kivanç BH, Görgül G. Staining Potential of Calcium Hydroxide and Monochlorophenol Following Removal of AH26 Root Canal Sealer. J Contemp Dent Pract 2008 March; (9)3:056-063.

scholarly journals Właściwości alkalizujące wybranych krzemianowo-wapniowych uszczelniaczy kanałowych. Badanie in vitro

2018 ◽  
Vol 64 (4) ◽  
Author(s):  
Przemysław Reszka ◽  
Łukasz Kucharski ◽  
Adam Klimowicz ◽  
Mariusz Lipski
Keyword(s):  
Root Canal ◽  
Calcium Silicate ◽  
In Vitro Study ◽  
Root Canal Sealer ◽  
Ph Values ◽  
Water Ph ◽  
Ah Plus ◽  
Ph Level ◽  
Root Canal Sealers

ABSTRACTIntroduction: The aim of this in vitro study was to assess the pH values of selected calcium-silicate root canal sealers in comparison with AH Plus resin-based root canal sealer.Materials and methods: Six root canal sealers were included in this study: BioRoot RCS (Septodont, France), GuttaFlow bioseal (Coltėne/Whaledent, Switzerland), MTA Fillapex (Angelus, Brazil), Total Fill BC Sealer (FKG, Switzerland), Well-Root ST (Vericom, Korea) and AH Plus (Dentsply, DeTrey, Germany). Materials samples (n = 6) were placed in dishes and immersed in 10 mL of distilled water (pH = 6.8). After 1, 3, 24, 48, 72, 168, 336 and 504 hrs, water pH was determined with a pH meter calibrated with a solution of known pH. Data were statistically analyzed using Student-t test (p < 0.05).Results: There were differences in the water pH between the materials tested. The highest water pHs were exhibited by Total Fill BC Sealer, Well-Root ST and BioRoot RCS (pH ~12), followed by GuttaFlow bioseal (pH ~10.5) and MTA Fillapex (pH ~9). The lowest pH was shown by AH Plus (pH ~8).Conclusions: All calcium-silicate root canal sealers studied are able to elevate the pH level, which could have biological and antimicrobial effects. The AH Plus root canal sealer has poor alkalizing properties.

scholarly journals Dentinal Tubule Penetration of a Calcium Silicate-Based Root Canal Sealer Using a Specific Calcium Fluorophore

2020 ◽  
Vol 31 (2) ◽  
pp. 109-115
Author(s):  
Viviane Siqueira Coronas ◽  
Natália Villa ◽  
Angela Longo do Nascimento ◽  
Pedro Henrique Marks Duarte ◽  
Ricardo Abreu da Rosa ◽  
...  
Keyword(s):  
Epoxy Resin ◽  
Root Canal ◽  
Laser Scanning ◽  
Root Apex ◽  
Laser Scanning Microscopy ◽  
Confocal Laser ◽  
Dentinal Tubule ◽  
Maxillary Molars ◽  
Ah Plus ◽  
Tubule Penetration

Abstract This study aimed to evaluate penetrability on dentinal tubule of a new bioceramic sealer through confocal laser scanning microscopy (CLSM). A specific fluorophore (Fluo-3) was mixed with the sealer. Forty distobuccal roots from maxillary molars were selected, and root canal preparation was carried out with Wave One Gold # 35.06 instruments. Roots were randomly assigned to 4 groups according to the filling procedures: Bioceramic/Lentulo (Sealer Plus BC); Bioceramic/EasyClean group, three activation of the sealer (3x20 s) with Easy Clean instrument; Bioceramic/Irrisonic: ultrasonic activation for 30 s; and AHplus/Lentulo: epoxy resin based sealer (AH Plus) was utilized with the same protocol as the BC/LE group. After 72 h, specimens were transversally sectioned at 2 and 7 mm from root apex and then analyzed through CLSM. Sealer penetration area on dentinal tubule was measured by Adobe Photoshop CC2018. Kruskal Wallis and Wilcoxon T tests were carried out. Penetrability results were similar for both sealers regardless of which technique was performed to activate them inside the root canal (p>0.05). It is reasonable to conclude that penetration of bioceramic and epoxy resin based sealers occurred unimpressively. The type of instrument used to activate bioceramic sealer did not affect penetrability. Fluo 3 should be recommended as the fluorophore to evaluate dentinal tubule penetration of bioceramic sealers.

scholarly journals Biocompatibility of a new epoxy resin-based root canal sealer in subcutaneous tissue of rat

2015 ◽  
Vol 09 (01) ◽  
pp. 031-035 ◽  
Author(s):  
Neslihan Simsek ◽  
Levent Akinci ◽  
Orhan Gecor ◽  
Hilal Alan ◽  
Fuat Ahmetoglu ◽  
...  
Keyword(s):  
Root Canal ◽  
Subcutaneous Tissue ◽  
Experimental Period ◽  
Tissue Reaction ◽  
Female Rats ◽  
Root Canal Sealer ◽  
Histologic Evaluation ◽  
Ah Plus ◽  
Rat Tissue ◽  
Root Canal Sealers

ABSTRACT Objective: The objective was to evaluate the subcutaneous biocompatibility of two root canal sealers. Materials and Methods: Thirty female rats were distributed into two groups of 15 animals. Each received subcutaneous dorsal implants: Silicone tubes filled with AH Plus or Obtuseal. After the 11th, 14th, and 45th days, tissues were collected for biopsy and fixed and processed for histologic evaluation. Observations of the cellular inflammatory components, such as lymphocytes and macrophages, were made. The data were analyzed using Kruskal–Wallis and Connover tests (P < 0.05). Results: By the 7th day, lymphocyte infiltration increased in both groups (P = 0.0104). However, macrophage infiltration increased only in the AH Plus group (P = 0.0011). By the 14th and 45th days, lymphocyte and macrophage infiltrations were reduced. At the end of the experimental period, no statistically significant differences were found between the AH Plus group and the Obtuseal group (P > 0.05). Conclusion: After 45 days, Obtuseal yielded a satisfactory tissue reaction; it was biocompatible when tested in subcutaneous rat tissue. On the basis of the obtained results, it was concluded that Obtuseal root canal sealer caused a satisfactory tissue reaction as AH plus, because it was biocompatible when tested in the subcutaneous tissue of rats.

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