scholarly journals High-resolution image-based simulation reveals membrane strain concentration on osteocyte processes caused by tethering elements

Author(s):  
Yuka Yokoyama ◽  
Yoshitaka Kameo ◽  
Hiroshi Kamioka ◽  
Taiji Adachi

AbstractOsteocytes are vital for regulating bone remodeling by sensing the flow-induced mechanical stimuli applied to their cell processes. In this mechanosensing mechanism, tethering elements (TEs) connecting the osteocyte process with the canalicular wall potentially amplify the strain on the osteocyte processes. The ultrastructure of the osteocyte processes and canaliculi can be visualized at a nanometer scale using high-resolution imaging via ultra-high voltage electron microscopy (UHVEM). Moreover, the irregular shapes of the osteocyte processes and the canaliculi, including the TEs in the canalicular space, should considerably influence the mechanical stimuli applied to the osteocytes. This study aims to characterize the roles of the ultrastructure of osteocyte processes and canaliculi in the mechanism of osteocyte mechanosensing. Thus, we constructed a high-resolution image-based model of an osteocyte process and a canaliculus using UHVEM tomography and investigated the distribution and magnitude of flow-induced local strain on the osteocyte process by performing fluid–structure interaction simulation. The analysis results reveal that local strain concentration in the osteocyte process was induced by a small number of TEs with high tension, which were inclined depending on the irregular shapes of osteocyte processes and canaliculi. Therefore, this study could provide meaningful insights into the effect of ultrastructure of osteocyte processes and canaliculi on the osteocyte mechanosensing mechanism.

Author(s):  
Robert M. Glaeser

It is well known that a large flux of electrons must pass through a specimen in order to obtain a high resolution image while a smaller particle flux is satisfactory for a low resolution image. The minimum particle flux that is required depends upon the contrast in the image and the signal-to-noise (S/N) ratio at which the data are considered acceptable. For a given S/N associated with statistical fluxtuations, the relationship between contrast and “counting statistics” is s131_eqn1, where C = contrast; r2 is the area of a picture element corresponding to the resolution, r; N is the number of electrons incident per unit area of the specimen; f is the fraction of electrons that contribute to formation of the image, relative to the total number of electrons incident upon the object.


Author(s):  
J. A. Pollock ◽  
M. Martone ◽  
T. Deerinck ◽  
M. H. Ellisman

Localization of specific proteins in cells by both light and electron microscopy has been facilitate by the availability of antibodies that recognize unique features of these proteins. High resolution localization studies conducted over the last 25 years have allowed biologists to study the synthesis, translocation and ultimate functional sites for many important classes of proteins. Recently, recombinant DNA techniques in molecular biology have allowed the production of specific probes for localization of nucleic acids by “in situ” hybridization. The availability of these probes potentially opens a new set of questions to experimental investigation regarding the subcellular distribution of specific DNA's and RNA's. Nucleic acids have a much lower “copy number” per cell than a typical protein, ranging from one copy to perhaps several thousand. Therefore, sensitive, high resolution techniques are required. There are several reasons why Intermediate Voltage Electron Microscopy (IVEM) and High Voltage Electron Microscopy (HVEM) are most useful for localization of nucleic acids in situ.


Author(s):  
Benjamin M. Siegel

The potential advantages of high voltage electron microscopy for extending the limits of resolution and contrast in imaging low contrast objects, such as biomolecular specimens, is very great. The results of computations will be presented showing that at accelerating voltages of 500-1000 kV it should be possible to achieve spacial resolutions of 1 to 1.5 Å and using phase contrast imaging achieve adequate image contrast to observe single atoms of low atomic number.The practical problems associated with the design and utilization of the high voltage instrument are, optimistically, within the range of competence of the state of the art. However, there are some extremely important and critical areas to be systematically investigated before we have achieved this competence. The basic electron optics of the column required is well understood, but before the full potential of an instrument capable of resolutions of better than 1.5 Å are realized some very careful development work will be required. Of great importance for the actual achievement of high resolution with a high voltage electron microscope is the fundamental limitation set by the characteristics of the high voltage electron beam that can be obtained from the accelerator column.


Author(s):  
А.С. Алексеев ◽  
А.А. Никифоров ◽  
А.А. Михайлова ◽  
М.Р. Вагизов

В связи со старением информационных материалов о состоянии лесов существует потребность в разработке новых методов таксации древостоев, основанных на применении последних научно-технических достижений в области теории структуры и продуктивности древостоев, дистанционных методов изучения лесов, информационных и ГИС технологий. В статье приведены результаты разработки и проверки нового метода определения таксационных характеристик сомкнутых насаждений на основе правила 3/2 и подобных ему правил Хильми и Рейнеке, с одной стороны, и определения числа деревьев на единице площади по снимку сверх высокого разрешения, полученного с помощью БПЛА, с другой. С теоретической точки зрения эта зависимости величин запаса, средней высоты и среднего диаметра от числа стволов на единице площади относятся к классу аллометрических связей, очень часто встречающихся при количественном описании соотношений частей биологических систем разных уровней иерархии, от организмов до экосистем. Параметры аллометрических зависимостей запаса, средних высоты и диаметра от числа стволов на единице площади были определены для основных лесообразующих пород по данным таблиц хода роста нормальных (полных) древостоев с теоретическим показателем степени и затем использованы для расчетов. Число деревьев на единице площади определялось по снимку с разрешением 7,13 см/пиксель, полученному с помощью 4-роторной платформы. Обработка материалов аэрофотосъемки была выполнена в специализированной фотограмметрической системе Agisoft Photoscan. В результате были получены ортофотоплан и цифровая модель поверхности крон деревьев на изучаемую территорию с определением их высот. Для автоматизированной обработки полученных изображений с целью получения значений числа деревьев на единицу площади был создан специализированный скрипт на языке Java. Погрешности определения таксационных характеристик древостоев предлагаемым методом не выше установленных действующими нормативными материалами. Every time there is a demand for new innovative methods of forest resources estimation based on last achievements in theoretical science, remote sensing methods, information and GIS-technologies. In the paper are presented a new method and the results of its application to forest stands growing stock, mean height and diameter determination. The method is based on rule 3/2 and similar Reineke and Hilmy rules, on one hand and high resolution image made by unmanned aerial vehicle, which used for determination of number of trees per area unit, on other. The above rules are well known in quantitative biology as an allometric and widely used for description of different kind of relations in biological systems of various scale: from organisms to ecosystems. Parameters of above allometric relationships between growing stock, mean height and diameter and stems density per area unit was determine on the base of full stock growth and yield tables for main tree species and after used for experimental calculations. The number of trees per area unit was determined after special treatment of high resolution image made by unmanned flying machine. The growing stock, mean height and diameter determined by suggested method was compared with the data of regular forest inventory. Comparison gives positive result and method may be recommended for further development.


1989 ◽  
Vol 281 (5) ◽  
pp. 336-341 ◽  
Author(s):  
W. Stolz ◽  
K. Scharffetter ◽  
W. Abmayr ◽  
W. K�ditz ◽  
T. Krieg

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