Acinetobacter lipases: molecular biology, biochemical properties and biotechnological potential

2004 ◽  
Vol 31 (9) ◽  
pp. 391-400 ◽  
Author(s):  
Erick A. Snellman ◽  
Rita R. Colwell
Keyword(s):  
Molecular Biology ◽  
Biochemical Properties ◽  
Biotechnological Potential

Cold-Active β-Galactosidases: Sources, Biochemical Properties and Their Biotechnological Potential

2016 ◽  
pp. 445-469 ◽  
Author(s):  
Hubert Cieśliński ◽  
Marta Wanarska ◽  
Anna Pawlak-Szukalska ◽  
Ewelina Krajewska ◽  
Monika Wicka ◽  
...  
Keyword(s):  
Biochemical Properties ◽  
Biotechnological Potential ◽  
Cold Active

scholarly journals Novel kit for efficient extraction of long-segment genomic DNA by nanodisk adsorption

2019 ◽  
Vol 1 (1) ◽  
pp. 4-5
Author(s):  
Siqi Wu ◽  
Zhaofang Han ◽  
Hesong Qiu
Keyword(s):  
Molecular Biology ◽  
Dna Extraction ◽  
High Purity ◽  
Genomic Dna ◽  
Extraction Methods ◽  
Biochemical Properties ◽  
High Efficacy ◽  
Tissue Samples ◽  
Efficient Extraction ◽  
Total Dna

The acquisition of complete DNA is usually the first and most critical step in many basic molecular biology applications. Isolation of complete total DNA from tissue samples is related to different physical and biochemical properties of tissue. Long fragments of DNA are easily degraded and fragmented, which poses a challenge for extracting complete long fragments of DNA. By comparing several different extraction methods and verifying the concentration and length of extracted DNA, it is proved that the kit we used has high  efficacy. We provide tissue kit DNA extraction for long fragments and obtain long fragments of high purity DNA from almost any type of tissue, especially muscle and blood tissues.

scholarly journals Snake Venom L-Amino Acid Oxidases: Trends in Pharmacology and Biochemistry

2014 ◽  
Vol 2014 ◽  
pp. 1-19 ◽  
Author(s):  
Luiz Fernando M. Izidoro ◽  
Juliana C. Sobrinho ◽  
Mirian M. Mendes ◽  
Tássia R. Costa ◽  
Amy N. Grabner ◽  
...  
Keyword(s):  
Amino Acid ◽  
Platelet Aggregation ◽  
Enzymatic Activity ◽  
Snake Venom ◽  
Biochemical Properties ◽  
Mechanisms Of Action ◽  
Pharmacological Effects ◽  
Biotechnological Potential ◽  
Structure Activity ◽  
Induction Of Apoptosis

L-amino acid oxidases are enzymes found in several organisms, including venoms of snakes, where they contribute to the toxicity of ophidian envenomation. Their toxicity is primarily due to enzymatic activity, but other mechanisms have been proposed recently which require further investigation. L-amino acid oxidases exert biological and pharmacological effects, including actions on platelet aggregation and the induction of apoptosis, hemorrhage, and cytotoxicity. These proteins present a high biotechnological potential for the development of antimicrobial, antitumor, and antiprotozoan agents. This review provides an overview of the biochemical properties and pharmacological effects of snake venom L-amino acid oxidases, their structure/activity relationship, and supposed mechanisms of action described so far.

scholarly journals Isolation, identification and investigation some biochemical properties of lactic acid bacteria from “Mam sac chua” and “Com me” in Tien Giang province

2021 ◽  
Vol 11 (1) ◽  
pp. 11-20
Author(s):  
Truong Quoc Tat ◽  
Nguyen Duy Khanh
Keyword(s):  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
Molecular Biology ◽  
Lactobacillus Plantarum ◽  
Salinity Tolerance ◽  
Salt Concentration ◽  
Biochemical Properties ◽  
Gram Positive ◽  
Molecular Biology Technique ◽  
Mrs Broth

The objective of this study was isolate, identify and investigate some biochemical properties of strains of lactic acid bacteria from “com me” and “mam sac chua” in Tien Giang province. Nineteen strains of LAB were isolated from four “com me” and three “mam sac chua” samples. They have characterized of lactic acid bacteria such as: halo rings in MRS agar environment added 0,85% CaCO3, rod-shaped cells, Gram positive, catalate and oxidase negative. All 19 strains of LAB were able to produce lactic acid in MRS broth (1,01 – 2,23 mg/ mL after 24 hours). Three strains of LAB were isolated from “com me” were able to produce lactic acid in MRS broth at salt concentration of 0, 2, 4 and 6% (0,57 – 1,29 mg/ mL after 24 hours). In particular, strains of LAB were coded ML3 and ML4 produced the highest lactic acid and VB strain was the most salinity tolerance. Therefore, these three srains were choosed to identify species by molecular biology technique. The results of identification were Staphylococcus piscifermentans VB, Lactobacillus plantarum ML3 and Lactobacillus plantarum ML4 because they are 99% homologous to S. piscifermentans and L. plantarum.

Electron Microscopy in Molecular Biology

1967 ◽  
Vol 25 ◽  
pp. 2-3
Author(s):  
Cecil E. Hall
Keyword(s):  
Electron Microscopy ◽  
Molecular Biology ◽  
Noise Level ◽  
Molecular Structures ◽  
Material Structure ◽  
The Arts ◽  
Shadow Casting ◽  
Electron Image ◽  
High Degree ◽  
Very High

The visualization of organic macromolecules such as proteins, nucleic acids, viruses and virus components has reached its high degree of effectiveness owing to refinements and reliability of instruments and to the invention of methods for enhancing the structure of these materials within the electron image. The latter techniques have been most important because what can be seen depends upon the molecular and atomic character of the object as modified which is rarely evident in the pristine material. Structure may thus be displayed by the arts of positive and negative staining, shadow casting, replication and other techniques. Enhancement of contrast, which delineates bounds of isolated macromolecules has been effected progressively over the years as illustrated in Figs. 1, 2, 3 and 4 by these methods. We now look to the future wondering what other visions are waiting to be seen. The instrument designers will need to exact from the arts of fabrication the performance that theory has prescribed as well as methods for phase and interference contrast with explorations of the potentialities of very high and very low voltages. Chemistry must play an increasingly important part in future progress by providing specific stain molecules of high visibility, substrates of vanishing “noise” level and means for preservation of molecular structures that usually exist in a solvated condition.

An introduction to the themed issue on RNA biology in China

2020 ◽  
Vol 64 (6) ◽  
pp. 863-866
Author(s):  
Zhe Wu
Keyword(s):  
Molecular Biology ◽  
Chinese Society ◽  
Fortieth Anniversary ◽  
Rna Biology ◽  
Research And Education ◽  
Education In China

Abstract The year 2019 marked the fortieth anniversary of the Chinese Society of Biochemistry and Molecular Biology (CSBMB), whose mission is to promote biomolecular research and education in China. The last 40 years have witnessed tremendous growth and achievements in biomolecular research by Chinese scientists and Essays in Biochemistry is delighted to publish this themed issue that focuses on exciting areas within RNA biology, with each review contributed by key experts from China.

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