scholarly journals Ribosome engineering and fermentation optimization leads to overproduction of tiancimycin A, a new enediyne natural product from Streptomyces sp. CB03234

2018 ◽  
Vol 45 (3) ◽  
pp. 141-151 ◽  
Author(s):  
Ling Liu ◽  
Jian Pan ◽  
Zilong Wang ◽  
Xiaohui Yan ◽  
Dong Yang ◽  
...  
Keyword(s):  
Natural Product ◽  
Fermentation Optimization ◽  
Ribosome Engineering ◽  
Streptomyces Sp

scholarly journals Draft Genome Sequence of Streptomyces sp. Strain JV178, a Producer of Clifednamide-Type Polycyclic Tetramate Macrolactams

2018 ◽  
Vol 6 (1) ◽  
Author(s):  
Yunci Qi ◽  
John M. D’Alessandro ◽  
Joshua A. V. Blodgett
Keyword(s):  
Natural Product ◽  
Genome Sequence ◽  
Draft Genome ◽  
Draft Genome Sequence ◽  
Garden Soil ◽  
Protein Coding ◽  
Coding Sequences ◽  
Streptomyces Sp

ABSTRACT Here, we report the draft genome sequence of Streptomyces sp. JV178, a strain originating from Connecticut (USA) garden soil. This strain produces the polycyclic tetramate macrolactam compounds clifednamides A and B. The draft genome contains 10.65 Mb, 9,045 predicted protein coding sequences, and several natural product biosynthetic loci.

Isolierung von Venturicidin X, dem Aglycon der Venturicidine A und B aus Streptomyceten / Isolation of Venturicidin X, the Aglycon of Venturicidines A and B from Streptopyces sp

1994 ◽  
Vol 49 (7) ◽  
pp. 977-980 ◽  
Author(s):  
Hartmut Laatsch ◽  
Michael Kellner ◽  
Yong-Se Lee ◽  
Gerhard Wolf
Keyword(s):  
Natural Product ◽  
Broad Spectrum ◽  
Pathogenic Fungi ◽  
Plant Pathogenic Fungi ◽  
Streptomyces Sp ◽  
Highly Active ◽  
First Time

Abstract Venturicidin X (la ), the aglycon of the venturicidins A (1b) and B (1c) was isolated for the first time as a natural product from an unidentified Streptomyces sp. Venturicidin X (1a) is highly active against a broad spectrum of plant pathogenic fungi.

Coordinated Action of NRPS, Baeyer-Villiger Monooxygenase, and Methyltransferase Ensures the Economical Biosynthesis of Bohemamines in Streptomyces sp. CB02009

2020 ◽  
Author(s):  
Ling Liu ◽  
Sainan Li ◽  
Runze Sun ◽  
Xiangjing Qin ◽  
Jianhua Ju ◽  
...  
Keyword(s):  
Methyl Groups ◽  
Ribosome Engineering ◽  
Methyl Group ◽  
Proteinogenic Amino Acid ◽  
Streptomyces Sp ◽  
Coordinated Action ◽  
Peptide Synthetase ◽  
Identification And Characterization ◽  
Shed Light

<p> Bohemamines (BHMs) are bacterial alkaloids containing a pyrrolizidine core with two unprecedented methyl groups. Herein we report the activation of BHMs biosynthesis in <i>Streptomyces </i>sp. CB02009 using a ribosome engineering approach. Identification and characterization of the <i>bhm</i> gene cluster reveals a coordinated action of nonribosomal peptide synthetase BhmJ, Baeyer-villiger monooxygenase BhmK and methyltransferase BhmG for BHMs biosynthesis. BhmG is responsible for the C-methylation on C-7, while the C-9 methyl group is from a non-proteinogenic amino acid (2<i>S</i>,5<i>S</i>)-5-methylproline, required for BHMs production in three model <i>Streptomyces </i>hosts. Our study shed light on the intricate interaction of BhmJ/BhmK/BhmG for the economical biosynthesis of BHMs in their native producer, and also unraveled that BhmJ and BhmK are competent biocatalysts in <i>S</i><i>treptomyce </i><i>albus</i>.</p>

scholarly journals New Kendomycin Derivative Isolated from Streptomyces sp. Cl 58-27

Molecules ◽  
2021 ◽  
Vol 26 (22) ◽  
pp. 6834
Author(s):  
Constanze Paulus ◽  
Oleksandr Gromyko ◽  
Andriy Luzhetskyy
Keyword(s):  
Nmr Spectroscopy ◽  
Natural Product ◽  
Gene Clusters ◽  
Biosynthetic Gene Cluster ◽  
Biosynthetic Gene ◽  
Streptomyces Sp ◽  
Bioinformatic Tools ◽  
Isolation And Characterization ◽  
Metabolism Gene

In the course of screening new streptomycete strains, the strain Streptomyces sp. Cl 58-27 caught our attention due to its interesting secondary metabolite production profile. Here, we report the isolation and characterization of an ansamycin natural product that belongs structurally to the already known kendomycins. The structure of the new kendomycin E was elucidated using NMR spectroscopy, and the corresponding biosynthetic gene cluster was identified by sequencing the genome of Streptomyces sp. Cl 58-27 and conducting a detailed analysis of secondary metabolism gene clusters using bioinformatic tools.

scholarly journals Complete genome sequences of Streptomyces spp. isolated from disease-suppressive soils

2019 ◽  
Author(s):  
Stephen C Heinsch ◽  
Suzie Hsu ◽  
Lindsey Otto-Hanson ◽  
Linda Kinkel ◽  
Michael Smanski
Keyword(s):  
Microbial Communities ◽  
Natural Product ◽  
De Novo ◽  
Sequence Similarity ◽  
Gene Clusters ◽  
Sequencing Data ◽  
Sample Number ◽  
Genus Streptomyces ◽  
Streptomyces Sp ◽  
Streptomyces Spp

Abstract Background Bacteria within the genus Streptomyces remain a major source of new natural product discovery and as soil inoculants in agriculture where they promote plant growth and protect from disease. Recently, Streptomyces spp. have been implicated as important members of naturally disease-suppressive soils. To shine more light on the ecology and evolution of disease-suppressive microbial communities, we have sequenced the genome of three Streptomyces strains isolated from disease-suppressive soils and compared them to previously sequenced isolates. Strains selected for sequencing had previously showed strong phenotypes in competition or signaling assays. Results Here we present the de novo sequencing of three strains of the genus Streptomyces isolated from disease-suppressive soils to produce high-quality complete genomes. Streptomyces sp. GS93-23, Streptomyces sp. 3211-3, and Streptomyces sp. S3-4 were found to have linear chromosomes of 8.24 Mb, 8.23 Mb, and greater than 7.5 Mb, respectively. In addition, two of the strains were found to have large, linear plasmids. Each strain harbors between 26 and 38 natural product biosynthetic gene clusters, on par with previously sequenced Streptomyces spp.. We compared these newly-sequenced genomes with those of previously sequenced organisms. We see substantial natural product biosynthetic diversity between closely related strains, with the gain/loss of episomal DNA elements being a primary driver of genome evolution. Conclusions Long read sequencing data facilitates large contig assembly for high-GC Streptomyces genomes. While the sample number is too small for a definitive conclusion, we do not see evidence that disease suppressive soil isolates are particularly privileged in terms of numbers of BGCs. The strong sequence similarity between GS93-23 and previously isolated Streptomyces lydicus suggests that species recruitment may contribute to the evolution of disease-suppressive microbial communities.

scholarly journals Characterization and fermentation optimization of novel thermo stable alkaline protease from Streptomyces sp. Al-Dhabi-82 from the Saudi Arabian environment for eco-friendly and industrial applications

2020 ◽  
Vol 32 (1) ◽  
pp. 1258-1264 ◽  
Author(s):  
Naif Abdullah Al-Dhabi ◽  
Galal Ali Esmail ◽  
Abdul-Kareem Mohammed Ghilan ◽  
Mariadhas Valan Arasu ◽  
Veeramuthu Duraipandiyan ◽  
...  
Keyword(s):  
Alkaline Protease ◽  
Saudi Arabian ◽  
Industrial Applications ◽  
Fermentation Optimization ◽  
Streptomyces Sp

scholarly journals Total Synthesis of 4-Acetyl-1,3-Dihydroimidazo[4,5-c]Pyridin-2-One, a New Microbial Metabolite from a Streptomyces Species

2009 ◽  
Vol 4 (7) ◽  
pp. 1934578X0900400 ◽  
Author(s):  
Tobias Bender ◽  
Paultheo von Zezschwitz
Keyword(s):  
Total Synthesis ◽  
Natural Product ◽  
Secondary Metabolite ◽  
Pyridine Derivative ◽  
Stille Reaction ◽  
Streptomyces Species ◽  
Streptomyces Sp ◽  
Microbial Metabolite

The structure of a new secondary metabolite from Streptomyces sp. was determined as 4-acetyl-1,3-dihydroimidazo[4,5-c]pyridin-2-one by synthesis of the natural product itself and of the regioisomeric 7-acetylimidazo[4,5-b]pyridine derivative. The former compound was prepared, in 28% overall yield, in a sequence of nitration, reduction, condensation, and Stille reaction of 4-aminopyridine, while the regioisomer was obtained in 5% overall yield by amination, nitration, reduction, condensation, and oxidation of 4-ethylpyridine.

scholarly journals The Application of Ribosome Engineering to Natural Product Discovery and Yield Improvement in Streptomyces

Antibiotics ◽  
2019 ◽  
Vol 8 (3) ◽  
pp. 133 ◽  
Author(s):  
Saibin Zhu ◽  
Yanwen Duan ◽  
Yong Huang
Keyword(s):  
Natural Product ◽  
Ribosomal Proteins ◽  
Cost Effective ◽  
Gene Clusters ◽  
Product Formation ◽  
Biosynthetic Gene ◽  
Biosynthetic Gene Clusters ◽  
Yield Improvement ◽  
Ribosome Engineering ◽  
Natural Product Discovery

Microbial natural product drug discovery and development has entered a new era, driven by microbial genomics and synthetic biology. Genome sequencing has revealed the vast potential to produce valuable secondary metabolites in bacteria and fungi. However, many of the biosynthetic gene clusters are silent under standard fermentation conditions. By rational screening for mutations in bacterial ribosomal proteins or RNA polymerases, ribosome engineering is a versatile approach to obtain mutants with improved titers for microbial product formation or new natural products through activating silent biosynthetic gene clusters. In this review, we discuss the mechanism of ribosome engineering and its application to natural product discovery and yield improvement in Streptomyces. Our analysis suggests that ribosome engineering is a rapid and cost-effective approach and could be adapted to speed up the discovery and development of natural product drug leads in the post-genomic era.

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