Stawamycin, A New Pyrroloketoindane Natural Product From the Cultures of Streptomyces sp.

1995 ◽  
Vol 36 (32) ◽  
pp. 5699-5702 ◽  
Author(s):  
S Miao
Keyword(s):  
Natural Product ◽  
Streptomyces Sp

scholarly journals Draft Genome Sequence of Streptomyces sp. Strain JV178, a Producer of Clifednamide-Type Polycyclic Tetramate Macrolactams

2018 ◽  
Vol 6 (1) ◽  
Author(s):  
Yunci Qi ◽  
John M. D’Alessandro ◽  
Joshua A. V. Blodgett
Keyword(s):  
Natural Product ◽  
Genome Sequence ◽  
Draft Genome ◽  
Draft Genome Sequence ◽  
Garden Soil ◽  
Protein Coding ◽  
Coding Sequences ◽  
Streptomyces Sp

ABSTRACT Here, we report the draft genome sequence of Streptomyces sp. JV178, a strain originating from Connecticut (USA) garden soil. This strain produces the polycyclic tetramate macrolactam compounds clifednamides A and B. The draft genome contains 10.65 Mb, 9,045 predicted protein coding sequences, and several natural product biosynthetic loci.

Isolierung von Venturicidin X, dem Aglycon der Venturicidine A und B aus Streptomyceten / Isolation of Venturicidin X, the Aglycon of Venturicidines A and B from Streptopyces sp

1994 ◽  
Vol 49 (7) ◽  
pp. 977-980 ◽  
Author(s):  
Hartmut Laatsch ◽  
Michael Kellner ◽  
Yong-Se Lee ◽  
Gerhard Wolf
Keyword(s):  
Natural Product ◽  
Broad Spectrum ◽  
Pathogenic Fungi ◽  
Plant Pathogenic Fungi ◽  
Streptomyces Sp ◽  
Highly Active ◽  
First Time

Abstract Venturicidin X (la ), the aglycon of the venturicidins A (1b) and B (1c) was isolated for the first time as a natural product from an unidentified Streptomyces sp. Venturicidin X (1a) is highly active against a broad spectrum of plant pathogenic fungi.

scholarly journals New Kendomycin Derivative Isolated from Streptomyces sp. Cl 58-27

Molecules ◽  
2021 ◽  
Vol 26 (22) ◽  
pp. 6834
Author(s):  
Constanze Paulus ◽  
Oleksandr Gromyko ◽  
Andriy Luzhetskyy
Keyword(s):  
Nmr Spectroscopy ◽  
Natural Product ◽  
Gene Clusters ◽  
Biosynthetic Gene Cluster ◽  
Biosynthetic Gene ◽  
Streptomyces Sp ◽  
Bioinformatic Tools ◽  
Isolation And Characterization ◽  
Metabolism Gene

In the course of screening new streptomycete strains, the strain Streptomyces sp. Cl 58-27 caught our attention due to its interesting secondary metabolite production profile. Here, we report the isolation and characterization of an ansamycin natural product that belongs structurally to the already known kendomycins. The structure of the new kendomycin E was elucidated using NMR spectroscopy, and the corresponding biosynthetic gene cluster was identified by sequencing the genome of Streptomyces sp. Cl 58-27 and conducting a detailed analysis of secondary metabolism gene clusters using bioinformatic tools.

scholarly journals Complete genome sequences of Streptomyces spp. isolated from disease-suppressive soils

2019 ◽  
Author(s):  
Stephen C Heinsch ◽  
Suzie Hsu ◽  
Lindsey Otto-Hanson ◽  
Linda Kinkel ◽  
Michael Smanski
Keyword(s):  
Microbial Communities ◽  
Natural Product ◽  
De Novo ◽  
Sequence Similarity ◽  
Gene Clusters ◽  
Sequencing Data ◽  
Sample Number ◽  
Genus Streptomyces ◽  
Streptomyces Sp ◽  
Streptomyces Spp

Abstract Background Bacteria within the genus Streptomyces remain a major source of new natural product discovery and as soil inoculants in agriculture where they promote plant growth and protect from disease. Recently, Streptomyces spp. have been implicated as important members of naturally disease-suppressive soils. To shine more light on the ecology and evolution of disease-suppressive microbial communities, we have sequenced the genome of three Streptomyces strains isolated from disease-suppressive soils and compared them to previously sequenced isolates. Strains selected for sequencing had previously showed strong phenotypes in competition or signaling assays. Results Here we present the de novo sequencing of three strains of the genus Streptomyces isolated from disease-suppressive soils to produce high-quality complete genomes. Streptomyces sp. GS93-23, Streptomyces sp. 3211-3, and Streptomyces sp. S3-4 were found to have linear chromosomes of 8.24 Mb, 8.23 Mb, and greater than 7.5 Mb, respectively. In addition, two of the strains were found to have large, linear plasmids. Each strain harbors between 26 and 38 natural product biosynthetic gene clusters, on par with previously sequenced Streptomyces spp.. We compared these newly-sequenced genomes with those of previously sequenced organisms. We see substantial natural product biosynthetic diversity between closely related strains, with the gain/loss of episomal DNA elements being a primary driver of genome evolution. Conclusions Long read sequencing data facilitates large contig assembly for high-GC Streptomyces genomes. While the sample number is too small for a definitive conclusion, we do not see evidence that disease suppressive soil isolates are particularly privileged in terms of numbers of BGCs. The strong sequence similarity between GS93-23 and previously isolated Streptomyces lydicus suggests that species recruitment may contribute to the evolution of disease-suppressive microbial communities.

scholarly journals Total Synthesis of 4-Acetyl-1,3-Dihydroimidazo[4,5-c]Pyridin-2-One, a New Microbial Metabolite from a Streptomyces Species

2009 ◽  
Vol 4 (7) ◽  
pp. 1934578X0900400 ◽  
Author(s):  
Tobias Bender ◽  
Paultheo von Zezschwitz
Keyword(s):  
Total Synthesis ◽  
Natural Product ◽  
Secondary Metabolite ◽  
Pyridine Derivative ◽  
Stille Reaction ◽  
Streptomyces Species ◽  
Streptomyces Sp ◽  
Microbial Metabolite

The structure of a new secondary metabolite from Streptomyces sp. was determined as 4-acetyl-1,3-dihydroimidazo[4,5-c]pyridin-2-one by synthesis of the natural product itself and of the regioisomeric 7-acetylimidazo[4,5-b]pyridine derivative. The former compound was prepared, in 28% overall yield, in a sequence of nitration, reduction, condensation, and Stille reaction of 4-aminopyridine, while the regioisomer was obtained in 5% overall yield by amination, nitration, reduction, condensation, and oxidation of 4-ethylpyridine.

Isolation, Structure Elucidation and Activity of Anthracycline Acetates from a Terrestrial Streptomyces sp.

2003 ◽  
Vol 58 (12) ◽  
pp. 1242-1246 ◽  
Author(s):  
Serge Fotso ◽  
Rajendra P. Maskey ◽  
Iris Grün-Wollny ◽  
Hartmut Laatsch
Keyword(s):  
Molecular Weight ◽  
Natural Product ◽  
Structure Elucidation ◽  
Ethyl Acetate Extract ◽  
2D Nmr ◽  
Streptomyces Sp ◽  
Naturally Occurring ◽  
Nmr Data ◽  
New Antibiotics ◽  
First Time

The red coloured ethyl acetate extract of the Streptomyces sp. isolate GW37/3236 delivered the two new antibiotics 13-O-acetyl-bisanhydro-13-dihydrodaunomycinone (3c) and 4,13-O-diacetylbisanhydro- 4-O-demethyl-13-dihydrodaunomycinone (3d) and additionally several known compounds. The quinones 3c and 3d are the first naturally occurring quinone acetates. Their structures were derived by comparison of the NMR data with those of bisanhydro-13-dihydrodaunomycinone (3b) and by interpretation of the 2D NMR data accompanied by the molecular weight and formula. 2-Acetamido-3-hydroxybenzamide (5) was also isolated from the extract and was identified by comparison of the NMR data with those of 2-acetamidobenzamide and by 2D NMR correlations. 6,9,11- Trihydroxy-4-methoxy-5,12-naphthacenedione (4) is isolated for the first time as a natural product.

scholarly journals Application of Cas12j for Streptomyces editing and cluster activation

2021 ◽  
Author(s):  
Lee Ling Tan ◽  
Elena Heng ◽  
Nadiah Zulkarnain ◽  
Chuang Yan Leong ◽  
Veronica Ng ◽  
...  
Keyword(s):  
Natural Product ◽  
Francisella Tularensis ◽  
Genetic Manipulation ◽  
Tularensis Subsp ◽  
Streptomyces Sp ◽  
Natural Product Discovery ◽  
Type V ◽  
Strain Dependent ◽  
Cas Proteins

In recent years, CRISPR-Cas toolboxes for Streptomyces editing have rapidly accelerated natural product discovery and engineering. However, Cas efficiencies are also oftentimes strain dependent, subsequently a variety of Cas proteins would allow for flexibility and enable genetic manipulation within a wider range of Streptomyces strains. In this work, we have further expanded the Cas toolbox by presenting the first example of Cas12j mediated editing in Streptomyces sp. A34053. In our study, we have also observed significantly improved editing efficiencies with Acidaminococcus sp. Cas12j compared to Cas12a, Francisella tularensis subsp. novicida U112's type V-A Cas (FnCpf1).

scholarly journals Complete genome sequences of Streptomyces spp. isolated from disease-suppressive soils

2019 ◽  
Author(s):  
Stephen C Heinsch ◽  
Suzie Hsu ◽  
Lindsey Otto-Hanson ◽  
Linda Kinkel ◽  
Michael Smanski
Keyword(s):  
Microbial Communities ◽  
Natural Product ◽  
De Novo ◽  
Sequence Similarity ◽  
Gene Clusters ◽  
Sequencing Data ◽  
Sample Number ◽  
Genus Streptomyces ◽  
Streptomyces Sp ◽  
Streptomyces Spp

Abstract Bacteria within the genus Streptomyces remain a major source of new natural product discovery and as soil inoculants in agriculture where they promote plant growth and protect from disease. Recently, Streptomyces spp. have been implicated as important members of naturally disease-suppressive soils. To shine more light on the ecology and evolution of disease-suppressive microbial communities, we have sequenced the genome of three Streptomyces strains isolated from disease-suppressive soils and compared them to previously sequenced isolates. Strains selected for sequencing had previously showed strong phenotypes in competition or signaling assays. Results Here we present the de novo sequencing of three strains of the genus Streptomyces isolated from disease-suppressive soils to produce high-quality complete genomes. Streptomyces sp. GS93-23, Streptomyces sp. 3211-3, and Streptomyces sp. S3-4 were found to have linear chromosomes of 8.24 Mb, 8.23 Mb, and greater than 7.5 Mb, respectively. In addition, two of the strains were found to have large, linear plasmids. Each strain harbors between 26 and 38 natural product biosynthetic gene clusters, on par with previously sequenced Streptomyces spp.. We compared these newly-sequenced genomes with those of previously sequenced organisms. We see substantial natural product biosynthetic diversity between closely related strains, with the gain/loss of episomal DNA elements being a primary driver of genome evolution. Conclusions Long read sequencing data facilitates large contig assembly for high-GC Streptomyces genomes. While the sample number is too small for a definitive conclusion, we do not see evidence that disease suppressive soil isolates are particularly privileged in terms of numbers of BGCs. The strong sequence similarity between GS93-23 and previously isolated Streptomyces lydicus suggests that species recruitment may contribute to the evolution of disease-suppressive microbial communities.

2-Alkyl-3,4-dihydroxy-5-hydroxymethylpyridine Derivatives: New Natural Vitamin B6 Analogues from a Terrestrial Streptomyces sp.

2005 ◽  
Vol 60 (1) ◽  
pp. 63-66 ◽  
Author(s):  
Rajendra P. Maskey ◽  
Felix Huth ◽  
Iris Grün-Wollny ◽  
Hartmut Laatsch
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Bacillus Subtilis ◽  
Candida Albicans ◽  
Natural Product ◽  
Anthranilic Acid ◽  
Vitamin B6 ◽  
Ethyl Acetate Extract ◽  
Mucor Miehei ◽  
Streptomyces Sp

The ethyl acetate extract of the strain Streptomyces sp. GW23/1540 has yielded four new 2-alkyl-5-(hydroxymethyl)pyridine-3,4-diols, 5-hydroxymethyl-2-isopropyl-pyridine-3,4-diol (1a), 5-hydroxymethyl-2-propyl-pyridine-3,4-diol (1b), 2-sec-butyl-5-hydroxymethyl-pyridine-3,4-diol (1c), and 5-hydroxymethyl-2-isobutyl-pyridine-3,4-diol (1d). Similarly, the strain Streptomyces sp. GW63/1571 afforded 2-sec-butyl-5-hydroxymethyl-pyridine-3,4-diol (1c) and another new natural product, (3aS, 7aR)-3a-hydroxy-3a,4,7,7a-tetrahydro-1-benzofuran-2(3H)-on e (3), together with anthranilic acid, anthranilamide, and phenylacetamide. The new natural products were inactive against three micro algae, the fungus Mucor miehei, the yeast Candida albicans, and the bacteria Staphylococcus aureus, Bacillus subtilis, Escherichia coli, and Streptomyces viridochromogenes.

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