2b Protein is essential to induce a novel gradual cell death in Zucchini yellow mosaic virus-inoculated cucumber cotyledon co-infected with Cucumber mosaic virus

2005 ◽  
Vol 71 (4) ◽  
pp. 308-313 ◽  
Author(s):  
Bo-Song Ryang ◽  
Tadashi Matsumoto ◽  
Takashi Kobori ◽  
Yoshitaka Kosaka ◽  
Satoshi T. Ohki
Keyword(s):  
Cell Death ◽  
Cucumber Mosaic Virus ◽  
Mosaic Virus ◽  
Zucchini Yellow Mosaic Virus ◽  
Yellow Mosaic Virus ◽  
Cucumber Cotyledon ◽  
2B Protein

scholarly journals Functional analysis of the Cucumber mosaic virus 2b protein: pathogenicity and nuclear localization

2004 ◽  
Vol 85 (10) ◽  
pp. 3135-3147 ◽  
Author(s):  
Yongzeng Wang ◽  
Tzvi Tzfira ◽  
Victor Gaba ◽  
Vitaly Citovsky ◽  
Peter Palukaitis ◽  
...  
Keyword(s):  
Cucumber Mosaic Virus ◽  
Mosaic Virus ◽  
Nuclear Localization ◽  
Zucchini Yellow Mosaic Virus ◽  
Yellow Mosaic Virus ◽  
Silencing Suppressor ◽  
Nuclear Localization Signals ◽  
Systemic Movement ◽  
2B Protein ◽  
Function Expression

The 2b protein encoded by Cucumber mosaic virus (CMV) has been shown to be a silencing suppressor and pathogenicity determinant in solanaceous hosts, but a movement determinant in cucumber. In addition, synergistic interactions between CMV and Zucchini yellow mosaic virus (ZYMV) have been described in several cucurbit species. Here, it was shown that deletion of the 2b gene from CMV prevented extensive systemic movement of the virus in zucchini squash, which could not be complemented by co-infection with ZYMV. Thus, ZYMV expressing a silencing suppressor with a different target could not complement the CMV 2b-specific movement function. Expression of the 2b protein from an attenuated ZYMV vector resulted in a synergistic response, largely restoring infection symptoms of wild-type ZYMV in several cucurbit species. Deletion or alteration of either of two nuclear localization signals (NLSs) did not affect nuclear localization in two assays, but did affect pathogenicity in several cucurbit species, whilst deletion of both NLSs led to loss of nuclear localization. The 2b protein interacted with an Arabidopsis thaliana karyopherin α protein (AtKAPα) in the yeast two-hybrid system, as did each of the two single NLS-deletion mutants. However, 2b protein containing a deletion of both NLSs was unable to interact with AtKAPα. These data suggest that the 2b protein localizes to the nucleus by using the karyopherin α-mediated system, but demonstrate that nuclear localization was insufficient for enhancement of the 2b-mediated pathogenic response in cucurbit hosts. Thus, the sequences corresponding to the two NLSs must have another role leading to pathogenicity enhancement.

scholarly journals First Report of Infection of Fallopia multiflora with cucumber mosaic virus and zucchini yellow mosaic virus

2018 ◽  
Vol 100 (2) ◽  
pp. 333-333 ◽  
Author(s):  
Nam-Yeon Kim ◽  
Hyo-Jeong Lee ◽  
Mi-Ri Park ◽  
Jin-Sung Hong ◽  
Rae-Dong Jeong
Keyword(s):  
Cucumber Mosaic Virus ◽  
Mosaic Virus ◽  
Zucchini Yellow Mosaic Virus ◽  
Yellow Mosaic Virus ◽  
First Report

scholarly journals 449 Cucurbit Viruses in Southern Illinois

HortScience ◽  
2000 ◽  
Vol 35 (3) ◽  
pp. 471B-471
Author(s):  
S. Alan Walters ◽  
Jeff D. Kindhart ◽  
Houston A. Hobbs ◽  
Darin M. Eastburn
Keyword(s):  
Cucumber Mosaic Virus ◽  
Mosaic Virus ◽  
Virus Disease ◽  
Zucchini Yellow Mosaic Virus ◽  
Growing Season ◽  
Yellow Mosaic Virus ◽  
Watermelon Mosaic Virus ◽  
Southern Illinois ◽  
Growing Seasons ◽  
Transgenic Squash

Cucurbit viruses are a major hindrance to cucurbit production in southern Illinois, often rendering cucumber and summer squash fruit unmarketable. Specific viruses infecting cucurbits in the region need to be determined since this would enable growers to make better decisions on virus disease management. Leaf samples of various cucurbit vegetables that had symptoms of viral infection were collected from grower fields during the 1998 and 1999 growing seasons to determine the predominant cucurbit viruses present. Samples were assayed for the presence of five individual viruses: cucumber mosaic virus (CMV), papaya ringspot virus (PRSV, formerly watermelon mosaic virus-1), squash mosaic virus (SqMV), watermelon mosaic virus (WMV, formerly watermelon mosaic virus-2), and zucchini yellow mosaic virus (ZYMV). Results from the two years indicated that WMV was the most prevalent virus in southern Illinois. Cucumber mosaic virus was found both years, but only in a low percentage of samples collected each year. Two cucurbit viruses, PRSV and ZYMV, were each identified only in one sample during the 1998 growing season, and neither were found in any of the samples collected during 1999. Squash mosaic virus was not identified in any of the samples collected during 1998; however, for the 1999 growing season, SqMV was identified in 19% of the samples collected, primarily from those samples of transgenic squash that were showing symptoms of virus infection.

scholarly journals Identificação sorológica de espécies de vírus que infetam cucurbitáceas em áreas produtoras do Maranhão

2001 ◽  
Vol 26 (1) ◽  
pp. 90-92 ◽  
Author(s):  
MARIA C. C. L. MOURA ◽  
J. ALBERSIO A. LIMA ◽  
VANÚZIA B. OLIVEIRA ◽  
M. FÁTIMA B. GONÇALVES
Keyword(s):  
Cucumber Mosaic Virus ◽  
Mosaic Virus ◽  
Immunosorbent Assay ◽  
Citrullus Lanatus ◽  
Zucchini Yellow Mosaic Virus ◽  
Yellow Mosaic Virus ◽  
Enzyme Linked Immunosorbent Assay ◽  
Watermelon Mosaic Virus ◽  
Papaya Ringspot Virus ◽  
Cucurbita Moschata

Os vírus representam sérios obstáculos para o sucesso da olericultura no mundo inteiro, constituindo a identificação daqueles de maior incidência numa região, papel fundamental para o estabelecimento de estratégias de controle. Visitas de campo foram realizadas a plantios de espécies de cucurbitáceas em áreas produtoras do Maranhão e amostras foliares foram coletadas de 118 plantas com sintomas ou suspeita de sintomas de vírus, sendo 46 de abóbora (Cucurbita moschata), 30 de melancia (Citrullus lanatus), 23 de maxixe (Cucumis anguria), 13 de pepino (C. sativus) e seis de melão (C. melo). Todas as amostras foram testadas contra anti-soros específicos para os principais vírus das famílias Bromoviridae, Comoviridae e Potyviridae que infetam cucurbitáceas no Nordeste, mediante "enzyme-linked immunosorbent assay" (ELISA) indireto e dupla difusão em agar. Os resultados revelaram a identificação sorológica de Papaya ringspot vírus (PRSV) em 64,4% das amostras analisadas, seguido de Watermelon mosaic virus-2 (WMV-2) em 15,2%, Cucumber mosaic virus (CMV) em 6,8%, Squash mosaic virus (SqMV) em 3,4% e Zucchini yellow mosaic virus (ZYMV) em 3,4%. Este levantamento confirma a predominância do PRSV em espécies de cucurbitáceas cultivadas no estado do Maranhão.

scholarly journals Characterization of Synergy Between Cucumber mosaic virus and Potyviruses in Cucurbit Hosts

2002 ◽  
Vol 92 (1) ◽  
pp. 51-58 ◽  
Author(s):  
Yongzeng Wang ◽  
Victor Gaba ◽  
Jie Yang ◽  
Peter Palukaitis ◽  
Amit Gal-On
Keyword(s):  
Cucumber Mosaic Virus ◽  
Mosaic Virus ◽  
Mixed Infection ◽  
Zucchini Yellow Mosaic Virus ◽  
Helper Virus ◽  
Synergistic Interaction ◽  
Yellow Mosaic Virus ◽  
Watermelon Mosaic Virus ◽  
Satellite Rnas ◽  
Rna Levels

Mixed infections of cucurbits by Cucumber mosaic virus (CMV) and potyviruses exhibit a synergistic interaction. Zucchini squash and melon plants coinfected by the potyvirus Zucchini yellow mosaic virus (ZYMV) and either Fny-CMV (subgroup IA) or LS-CMV (subgroup II) displayed strong synergistic pathological responses, eventually progressing to vascular wilt and plant death. Accumulation of Fny- or LS-CMV RNAs in a mixed infection with ZYMV in zucchini squash was slightly higher than infection with CMV strains alone. There was an increase in CMV (+) strand RNA levels, but no increase in CMV (-) RNA3 levels during mixed infection with ZYMV. Moreover, only the level of capsid protein from LS-CMV increased in mixed infection. ZYMV accumulated to similar levels in singly and mixed infected zucchini squash and melon plants. Coinfection of squash with the potyvirus Watermelon mosaic virus (WMV) and CMV strains increased both the Fny-CMV RNA levels and the LS-CMV RNA levels. However, CMV (-) strand RNA3 levels were increased little or not at all for CMV on coinfection with WMV. Infection of CMV strains (LS and Fny) containing satellite RNAs (WL47-sat RNA and B5*-sat RNA) reduced the accumulation of the helper virus RNA, except when B5*-sat RNA was mixed with LS- CMV. However, mixed infection containing ZYMV and the CMV strains with satellites reversed the suppression effect of satellite RNAs on helper virus accumulation and increased satellite RNA accumulation. The synergistic interaction between CMV and potyviruses in cucurbits exhibited different features from that documented in tobacco, indicating there are differences in the mechanisms of potyvirus synergistic phenomena.

scholarly journals Systemic movement of a movement-deficient strain of Cucumber mosaic virus in zucchini squash is facilitated by a cucurbit-infecting potyvirus

2002 ◽  
Vol 83 (12) ◽  
pp. 3173-3178 ◽  
Author(s):  
Seung Kook Choi ◽  
Ju Yeon Yoon ◽  
Ki Hyun Ryu ◽  
Jang Kyung Choi ◽  
Peter Palukaitis ◽  
...  
Keyword(s):  
Cucumber Mosaic Virus ◽  
Mosaic Virus ◽  
Cucurbita Pepo ◽  
Zucchini Yellow Mosaic Virus ◽  
Yellow Mosaic Virus ◽  
Long Distance ◽  
Systemic Movement ◽  
Tissue Print ◽  
Long Distance Movement

Zucchini squash (Cucurbita pepo) is a systemic host for most strains of the cucumovirus Cucumber mosaic virus (CMV), although the long-distance movement of the M strain of CMV (M-CMV) is inhibited in some cultivars. However, co-infection of zucchini plants with M-CMV and the potyvirus Zucchini yellow mosaic virus strain A (ZYMV-A) allowed M-CMV to move systemically, as demonstrated by tissue-print analysis. These doubly infected plants exhibited severe synergism in pathology. Infection of zucchini squash by M-CMV and an attenuated strain of ZYMV (ZYMV-AG) showed a milder synergy in pathology, in which ZYMV-AG also facilitated the long-distance movement of M-CMV similar to that promoted by ZYMV-A. Variation in the extent of synergy in pathology by the two strains of ZYMV did not correlate with differences in levels of accumulation of either virus. Thus, the extent of synergy in pathology is at least in part independent of the resistance-neutralizing function of the potyvirus.

scholarly journals Ocorrência de patógenos em cultivos de melancia e abóbora no sertão da Paraíba

2016 ◽  
Vol 11 (1) ◽  
pp. 07 ◽  
Author(s):  
M. G. F. O. Soares ◽  
J. A. Soares ◽  
M. A. Cezar ◽  
T. A. L. Cardoso ◽  
J. A. A. Lima
Keyword(s):  
Cucumber Mosaic Virus ◽  
Mosaic Virus ◽  
Zucchini Yellow Mosaic Virus ◽  
Morphological Characteristics ◽  
Yellow Mosaic Virus ◽  
Watermelon Mosaic Virus ◽  
Mixed Infections ◽  
Spot Virus ◽  
Papaya Ring Spot Virus ◽  
Ring Spot

<p>Por constituírem uma importante fonte de alimento,<strong> </strong>plantações de melancia e abóbora são comumente cultivadas no sertão paraibano, porém pouco se sabe sobre a ocorrência de patógenos causadores de doenças, os quais limitam a sua produtividade e renda aos produtores. Visando obter informações sobre a ocorrência dos patógenos virais e fúngicos em cultivos de abóbora e melancia situados em municípios produtores no sertão da Paraíba, amostras coletadas com sintomas de mosaico e deformação foliar, típicos de doenças virais foram analisadas pela técnica sorológica “enzime linked immuno sorbentassay” (Elisa) indireto para <em>Papaya ring spot virus, </em>type watermelon (PRSV-W), <em>Watermelon mosaic virus </em>(WMV), <em>Zucchini yellow mosaic virus </em>(ZYMV) e <em>Cucumber mosaic virus </em>(CMV). O teste de dupla difusão em Agar foi utilizado para verificar a presença de <em>Squash mosaic virus </em>(SqMV). Em contrapartida, amostras coletadas com sintomas de doenças fúngicas foram analisadas pelo isolamento do patógeno e visualização de suas características morfológicas em microscópio óptico. Em abóbora, houve prevalência dos vírus ZYMV e PRSV-W em infecções simples e mistas, e maior incidência dos fungos <em>Cladosporium</em> spp., e <em>Alternaria</em> spp. Em melancia detectou-se<em> </em>infecção simples e mistas das espécies PRSV-W, WMV e ZYMV, e maior freqüência de <em>Fusarium</em> spp. e <em>Alternaria</em> spp.. Não foram detectados os vírus CMV e SqMV. Os resultados obtidos revelam a ocorrência de vários patógenos fúngicos e viróticos em cultivos de abóbora e melancia situados no sertão da Paraíba e ressaltam a importância da utilização de estratégias de manejo que reduzem os danos ocasionados por esses patógenos.</p><p align="center"><strong><em>Occurrence of pathogens in watermelon and pumpkin crops in the State of Paraiba</em></strong></p><p><strong>Abstract:</strong> By constitute an important food source, watermelon and pumpkin plantations are commonly grown on Paraíba<strong> </strong>backlands, but little is known about the occurrence of disease-causing pathogens, which limit their productivity and income to producers. With objective to get information about the viral and fungal pathogens occurrence in pumpkin and watermelon crops in producing counties located in the backlands of Paraiba, samples with symptoms of mosaic and leaf distortion, typical of viral etiology of disease were collected and analyzed by "Enzyme linked immune sorbentassay" (Elisa) Indirect for species <em>Papaya ring spot virus</em>, type watermelon (PRSV-W), <em>Watermelon mosaic virus </em>(WMV), <em>Zucchini yellow mosaic virus</em> ( ZYMV) and <em>Cucumber mosaic virus</em> (CMV). The double diffusion test in Agar was used to verify the presence of <em>Squash mosaic virus</em> (SqMV). In contrast, samples collected with fungal disease symptoms were analyzed based on the isolation of the pathogen and viewing their morphological characteristics under an optical microscope. In pumpkin, there was prevalence of ZYMV and PRSV-W in single and mixed infections, and higher incidence of <em>Cladosporium</em> spp. and <em>Alternaria</em> spp. On the other hand, in watermelon was detected single and mixed infections of PRSV-W, WMV and ZYMV species, and higher frequency of <em>Fusarium </em>spp. and <em>Alternaria</em> spp.. Were not detected the virus CMV and SqMV. The results show the occurrence of various fungal and viral pathogens in pumpkin and watermelon crops located in the backlands of Paraiba and emphasize the importance of using management strategies that reduce the damage caused by these pathogens.</p>

Sign in / Sign up

Export Citation Format

Share Document