SuperSAGE revealed different classes of early resistance response genes in Capsicum chinense plants harboring L 3 -resistance gene infected with Pepper mild mottle virus

2008 ◽  
Vol 74 (4) ◽  
pp. 313-321 ◽  
Author(s):  
Hiroyuki Hamada ◽  
Hideo Matsumura ◽  
Reiko Tomita ◽  
Ryohei Terauchi ◽  
Kazumi Suzuki ◽  
...  
Keyword(s):  
Resistance Gene ◽  
Mottle Virus ◽  
Capsicum Chinense ◽  
Pepper Mild Mottle Virus ◽  
Resistance Response

scholarly journals Pepper Mild Mottle Virus Coat Protein Alone Can Elicit the Capsicum spp. L3 Gene-Mediated Resistance

1998 ◽  
Vol 11 (12) ◽  
pp. 1253-1257 ◽  
Author(s):  
P. Gilardi ◽  
I. García-Luque ◽  
M. T. Serra
Keyword(s):  
Coat Protein ◽  
Resistance Gene ◽  
Protein Gene ◽  
Expression System ◽  
Potato Virus X ◽  
Mottle Virus ◽  
Capsicum Chinense ◽  
Pepper Mild Mottle Virus ◽  
Capsicum Spp ◽  
Virus Coat

The pepper mild mottle virus (PMMoV-S) (an L3 hypersensitive response [HR]-inducer strain) coat protein was expressed in Capsicum chinense (L3L3) plants with the heterologous potato virus X (PVX)-based expression system. The chimeric virus was localized in the inoculated leaves and induced the HR, thus indicating that the tobamoviral sequences that affect induction of the HR conferred by the L3 resistance gene reside in the coat protein gene. Furthermore, transient expression of the PMMoV-S coat protein in C. chinense leaves by biolistic co-bombardment with a plasmid expressing the β-glucuronidase (GUS) gene leads to the induction of cell death and expression of host defense genes. Thus, the coat protein of PMMoV-S is the elicitor of the Capsicum spp. L3 resistance gene-mediated HR.

scholarly journals Identification of Pepper mild mottle virus in Commercial Bell Pepper in Florida

Plant Disease ◽  
2001 ◽  
Vol 85 (6) ◽  
pp. 679-679 ◽  
Author(s):  
S. Adkins ◽  
E. M. Lamb ◽  
P. D. Roberts ◽  
M. D. Gooch ◽  
L. Breman ◽  
...  
Keyword(s):  
South Florida ◽  
Bell Pepper ◽  
Mottle Virus ◽  
Capsicum Chinense ◽  
Careful Monitoring ◽  
Pepper Mild Mottle Virus ◽  
Double Stranded Rna ◽  
Growing Seasons ◽  
Yellow Mottling ◽  
Made In

Pepper mild mottle virus (PMMoV) has been identified on pepper in Florida several times since 1997. Initial observations were on ornamental peppers (Capsicum chinense, C. frutescens, and C. annuum). However, in the winter growing seasons of 1999 and 2000, PMMoV was detected in several bell pepper (C. annuum) cultivars in commercial production fields in south Florida. Symptoms in bell pepper plants were observed to vary with plant age and cultivar, and included stunting of young plants and puckering and yellow mottling of leaves. Fruit was deformed (lumpy and mottled) and slightly reduced in size. Older fruit exhibited brown streaks and, in some cultivars, an undesirable color at maturity. Incidence in commercial bell pepper fields varied from <1 to 30%. Symptoms on mechanically inoculated indicator hosts, analysis of viral-associated double-stranded RNA, and inclusion body morphology suggested the presence of a tobamovirus. PMMoV was specifically identified by serological testing using ELISA (Agdia, Elkhart, IN). To our knowledge, this is the first definitive identification of PMMoV (2) in Florida, although a prior isolation of the same or a similar virus was made in 1964 and reported as the Samsun latent strain of Tobacco mosaic virus (1). The occurrence of this seedborne virus in an area of intensive commercial pepper production requires careful monitoring to avoid future significant losses. References: (1) W. H. Greenleaf et al. Phytopathology 54:1367, 1964. (2) C. Wetter. Plant Dis. 68:597, 1984.

scholarly journals Studies on the Tobamovirus resistance of the pepper (Capsicum annuum L.) cultivar Greygo

2001 ◽  
Vol 7 (1) ◽  
Author(s):  
P. Salomon ◽  
G. Venczel
Keyword(s):  
Resistance Gene ◽  
Capsicum Annuum ◽  
Mottle Virus ◽  
Resistance Allele ◽  
Capsicum Frutescens ◽  
Pepper Mild Mottle Virus ◽  
Capsicum Annuum L

Resistance of the Hungarian pepper (Capsicum annuum L.) cultivar "Gre.ygo" to Tohamoviruses has been investigated. All plants of the population of Greygo proved to be resistant to tobacco mosaic and tomato mosaic viruses (TMV, ToMV), both represent the pepper pathotypes Po of Tohamoviruses. Individuals of Greygo, however, were found to be susceptible to pathotypes P12 and P123 of pepper mild mottle virus (PMMV). When inoculated with the XM isolate of dulcamara yellow fleck virus (DYFV, pathotype P1) the population of Greygo segregated in resistant and susceptible plants. These results as well as inoculations of the progenies of three TMV resistant plants clearly showed, that besides the resistance allele Li the cultivar Greygo possesses also an another allele. This allele, provisionally marked by L2g behaves like to the allele L2 characteristic to Capsicum frutescens cv. . Tabasco. Determination of the identity of the allele L2g to the allele L2 needs further genetic and pathological informations. Relations between the Tohamoviruses pathogenic to pepper and the alleles of the resistance gene L are outlined for the discussion.  

scholarly journals Two Amino Acid Substitutions in the Coat Protein of Pepper mild mottle virus Are Responsible for Overcoming the L4 Gene-Mediated Resistance in Capsicum spp.

2007 ◽  
Vol 97 (7) ◽  
pp. 787-793 ◽  
Author(s):  
Yoshikatsu Genda ◽  
Ayami Kanda ◽  
Hiroyuki Hamada ◽  
Kyoko Sato ◽  
Jun Ohnishi ◽  
...  
Keyword(s):  
Amino Acid ◽  
Coat Protein ◽  
Resistance Gene ◽  
Site Directed Mutagenesis ◽  
Mottle Virus ◽  
Amino Acid Substitutions ◽  
Pepper Mild Mottle Virus ◽  
Biological Assays ◽  
Capsicum Spp ◽  
Capsicum Chacoense

The Capsicum spp. L genes (L1 to L4) confer resistance to tobamoviruses. Currently, the L4 gene from Capsicum chacoense is the most effective resistance gene and has been used widely in breeding programs in Japan which have developed new resistant cultivars against Pepper mild mottle virus (PMMoV). However, in 2004, mild mosaic symptoms began appearing on the leaves of commercial pepper plants in the field which possessed the L4 resistance gene. Serological and biological assays on Capsicum spp. identified the causal virus strain as a previously unreported pathotype, P1,2,3,4. PMMoV sequence analysis of the virus and site-directed mutagenesis using a PMMoV-J of the P1,2 pathotype revealed that two amino acid substitutions in the coat protein, Gln to Arg at position 46 and Gly to Lys at position 85, were responsible for overcoming the L4 resistance gene.

scholarly journals The Coat Protein Is Required for the Elicitation of the Capsicum L2 Gene-Mediated Resistance Against the Tobamoviruses

1997 ◽  
Vol 10 (1) ◽  
pp. 107-113 ◽  
Author(s):  
A. de la Cruz ◽  
L. López ◽  
F. Tenllado ◽  
J. R. Díaz-Ruíz ◽  
A. I. Sanz ◽  
...  
Keyword(s):  
Coat Protein ◽  
Resistance Gene ◽  
Coat Protein Gene ◽  
Protein Gene ◽  
Hypersensitive Reaction ◽  
Mottle Virus ◽  
Pepper Mild Mottle Virus ◽  
Factors Affecting ◽  
Viral Genomes ◽  
Capsicum Spp

In Capsicum, the resistance against tobamoviruses conferred by the L2 gene is effective against all but one of the known tobamoviruses. Pepper mild mottle virus (PMMoV) is the only virus which escapes its action. To identify the viral factors affecting induction of the hypersensitive reaction (HR) mediated by the Capsicum spp. L2 resistance gene, we have constructed chimeric viral genomes between paprika mild mottle virus (PaMMV) (a virus able to induce the HR) and PMMoV. A hybrid virus with the PaMMV coat protein gene substituted in the PMMoV-S sequences was able to elicit the HR in Capsicum frutescens (L2L2) plants. These data indicate that the sequences that affect induction of the HR mediated by the L2 resistance gene reside in the coat protein gene. Furthermore, a mutant that codes for a truncated coat protein was able to systemically spread in these plants. Thus, the elicitation of the host response requires the coat protein and not the RNA.

Variability and evolution of the plant RNA virus pepper mild mottle virus.

1989 ◽  
Vol 63 (5) ◽  
pp. 2198-2203 ◽  
Author(s):  
E Rodríguez-Cerezo ◽  
A Moya ◽  
F García-Arenal
Keyword(s):  
Rna Virus ◽  
Mottle Virus ◽  
Pepper Mild Mottle Virus

scholarly journals First report of Chilli veinal mottle virus in Naga chilli (Capsicum chinense) in Meghalaya, India

VirusDisease ◽  
2013 ◽  
Vol 25 (1) ◽  
pp. 142-143 ◽  
Author(s):  
Amrita Banerjee ◽  
Ram Dutta ◽  
Somnath Roy ◽  
S. V. Ngachan
Keyword(s):  
Mottle Virus ◽  
Capsicum Chinense ◽  
First Report ◽  
Chilli Veinal Mottle Virus

scholarly journals The coat protein of tobamovirus acts as elicitor of both L 2 and L 4 gene-mediated resistance in Capsicum

2004 ◽  
Vol 85 (7) ◽  
pp. 2077-2085 ◽  
Author(s):  
P. Gilardi ◽  
I. García-Luque ◽  
M. T. Serra
Keyword(s):  
Hypersensitive Response ◽  
Host Response ◽  
Expression System ◽  
Potato Virus X ◽  
Mottle Virus ◽  
Coat Proteins ◽  
Pepper Mild Mottle Virus ◽  
Defence Genes ◽  
Glucuronidase Reporter ◽  
Capsicum Chacoense

In Capsicum, the resistance conferred by the L 2 gene is effective against all of the pepper-infecting tobamoviruses except Pepper mild mottle virus (PMMoV), whereas that conferred by the L 4 gene is effective against them all. These resistances are expressed by a hypersensitive response, manifested through the formation of necrotic local lesions (NLLs) at the primary site of infection. The Capsicum L 2 gene confers resistance to Paprika mild mottle virus (PaMMV), while the L 4 gene is effective against both PaMMV and PMMoV. The PaMMV and PMMoV coat proteins (CPs) were expressed in Capsicum frutescens (L 2 L 2) and Capsicum chacoense (L 4 L 4) plants using the heterologous Potato virus X (PVX)-based expression system. In C. frutescens (L 2 L 2) plants, the chimeric PVX virus containing the PaMMV CP was localized in the inoculated leaves and produced NLLs, whereas the chimeric PVX containing the PMMoV CP infected the plants systemically. Thus, the data indicated that the PaMMV CP is the only tobamovirus factor required for the induction of the host response mediated by the Capsicum L 2 resistance gene. In C. chacoense (L 4 L 4) plants, both chimeric viruses were localized to the inoculated leaves and produced NLLs, indicating that either PaMMV or PMMoV CPs are required to elicit the L 4 gene-mediated host response. In addition, transient expression of PaMMV CP into C. frutescens (L 2 L 2) leaves and PMMoV CP into C. chacoense (L 4 L 4) leaves by biolistic co-bombardment with a β-glucuronidase reporter gene led to the induction of cell death and the expression of host defence genes in both hosts. Thus, the tobamovirus CP is the elicitor of the Capsicum L 2 and L 4 gene-mediated hypersensitive response.

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