scholarly journals First report of Chilli veinal mottle virus in Naga chilli (Capsicum chinense) in Meghalaya, India

VirusDisease ◽  
2013 ◽  
Vol 25 (1) ◽  
pp. 142-143 ◽  
Author(s):  
Amrita Banerjee ◽  
Ram Dutta ◽  
Somnath Roy ◽  
S. V. Ngachan
Keyword(s):  
Mottle Virus ◽  
Capsicum Chinense ◽  
First Report ◽  
Chilli Veinal Mottle Virus

scholarly journals Natural Occurrence of Chilli veinal mottle virus on Capsicum chinense in China

Plant Disease ◽  
2006 ◽  
Vol 90 (3) ◽  
pp. 377-377 ◽  
Author(s):  
J. Wang ◽  
Z. Liu ◽  
S. Niu ◽  
M. Peng ◽  
D. Wang ◽  
...  
Keyword(s):  
Polyacrylamide Gel Electrophoresis ◽  
Sodium Dodecyl ◽  
Viral Disease ◽  
Mottle Virus ◽  
Major Protein ◽  
Natural Occurrence ◽  
Capsicum Chinense ◽  
Thin Sections ◽  
First Report ◽  
Chilli Veinal Mottle Virus

An outbreak of a viral disease on chili pepper (Capsicum chinense Jacp. cv. Yellow Lantern) occurred in Hainan Province, China during 2003 and 2004. The disease was prevalent in five chili-producing counties surveyed. Leaves of infected plants initially displayed symptoms of dark green banding along veins and later became distorted with striking mosaic. Infected plants had reduced flower numbers and fruit set, resulting in a significant yield loss. The causative virus was characterized and identified as Chilli veinal mottle virus (ChiVMV) (3). An isolate of the virus was obtained via three single lesion passages through Chenopodium amaranticolor and was shown to reproduce the same symptoms on inoculated C. chinense cv. Yellow Lantern. Negative staining of crude extracts of the infected tissue and subsequent electron microscopy revealed flexuous rods of 12 to 13 × 750 nm, typical of a potyvirus. Pinwheel-like inclusion bodies were abundant in thin sections of infected leaves. Purified virus preparations contained one major protein of 32.8 kDa and one minor protein of 28 kDa when fractionated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Both of these protein bands were excised and subsequently analyzed using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS). Multiple peptide fragments from both proteins were identified as arising from ChiVMV capsid protein (CP) (1,2). Therefore, the 32.8-kDa protein is the full-length ChiVMV CP and the 28-kDa protein is presumably a degradation product of the CP. The combined biological and molecular data provided strong evidence that the viral disease on C. chinense was caused by ChiVMV. To our knowledge, this is the first report of ChiVMV infection on C. chinense in China and the first report of C. amaranticolor as an experimental host for ChiVMV. References: (1) P. Chiemsombat et al. Arch. Virol. 143:1855, 1998. (2). J. Joseph and H. S. Savithri. Arch. Virol. 144:1679, 1999. (3) P. Siriwong et al. Plant Pathol. 44:718, 1995.

scholarly journals First Report of Chilli veinal mottle virus Infecting Tomato (Solanum lycopersicum) in China

Plant Disease ◽  
2014 ◽  
Vol 98 (11) ◽  
pp. 1589-1589 ◽  
Author(s):  
F.-F. Zhao ◽  
D.-H. Xi ◽  
J. Liu ◽  
X.-G. Deng ◽  
H.-H. Lin
Keyword(s):  
Mosaic Virus ◽  
Southwest China ◽  
Sichuan Province ◽  
Mottle Virus ◽  
Rt Pcr ◽  
Tomato Plants ◽  
First Report ◽  
Two Samples ◽  
Leaf Distortion ◽  
Chilli Veinal Mottle Virus

Chilli veinal mottle virus (ChiVMV), a potyvirus, is widespread over the world. In China, it was first reported in chili pepper (Capsicum annuum) in Hainan Province (south China) in 2006 (2). Subsequently, it was reported in tobacco (Nicotiana tabacum) in Yunnan Province (southwest China) in 2011 (1). Sichuan Province is one of the largest vegetable producing areas of China. In May 2012, tomatoes with leaves displaying virus-infected symptoms like mottling, mosaic, narrowing, or curling were observed in several fields of Chengdu, eastern Sichuan Province, southwest China. Of the 20 fields we investigated, four fields with 90% tomato plants were infected. During 2012 and 2013, six samples were collected from symptomatic tomato leaves based on different symptoms and locations. All six samples were assayed by western blotting using polyclonal antisera (Cucumber mosaic virus [CMV], Tobacco mosaic virus [TMV]) obtained from Agdia (Elkhart) and one antiserum to ChiVMV obtained from Yunnan Academy of Agricultural Science (China). Two samples from Pengzhou and one sample from Shuangliu exhibiting mosaic leaves were positive for TMV, one sample from Pixian exhibiting narrowing leaves was positive for CMV, and the other two samples from Shuangliu exhibiting mottle and leaf distortion were positive for ChiVMV. Total RNAs was extracted from all six samples and healthy tomato leaves using Trizol reagent (Invitrogen), First-strand cDNA synthesis primed with oligo(dT) by SuperScript III Reverse Transcriptase (Invitrogen). RT-PCR was performed using primer pairs ChiVMV-CP F (5′-GCAGGAGAGAGTGTTGATGCTG-3′) and ChiVMV–CP R (5′-(T)16AACGCCAACTATTG-3′), which were designed to direct the amplification of the entire capsid protein (CP) gene and 3′ untranslated region (3′-UTR) of ChiVMV (GenBank Accession No. KC711055). The expected 1,166-bp DNA fragment was amplified from the two tomato samples from Shuangliu that were positive for ChiVMV in the western blot tests, but not from the others. The obtained fragments were purified and cloned into the PMD18-T vector (TaKaRa) and sequenced. The sequencing results showed that the two ChiVMV isolates from tomato in Shuangliu were identical (KF738253). Nucleotide BLAST analysis revealed that this ChiVMV isolate shared ~84 to 99% nucleotide identities with other ChiVMV isolates available in GenBank (KC711055 to KF220408). To fulfill Koch's postulates, we isolated this virus by three cycle single lesion isolation in N. tabacum, and mechanically inoculated it onto tomato leaves. The same mottle and leaf distortion symptoms in systemic leaves were observed. Subsequent RT-PCR, fragment clone, and sequence determination tests were repeated and the results were the same. All the evidence from these tests revealed that the two tomato plants were infected by ChiVMV. To our knowledge, this is the first report of ChiVMV naturally infecting tomato in China. It shows that ChiVMV is spreading in China and is naturally infecting a new solanaceous crop in the southwest area, and the spread of the virus may affect tomato crop yields in China. Thus, it is very important to seek an effective way to control this virus. References: (1) M. Ding et al. Plant Dis. 95:357, 2011. (2) J. Wang et al. Plant Dis. 90:377, 2006.

scholarly journals First Report of Chilli veinal mottle virus Infecting Solanum aethiopicum in China

Plant Disease ◽  
2018 ◽  
Vol 102 (6) ◽  
pp. 1181-1181
Author(s):  
Y. Y. Li ◽  
Y. Ma ◽  
Y. Meng ◽  
M. Z. Huang ◽  
G. M. Ren ◽  
...  
Keyword(s):  
Mottle Virus ◽  
First Report ◽  
Solanum Aethiopicum ◽  
Chilli Veinal Mottle Virus

scholarly journals First Report of Chilli veinal mottle virus Infecting Lycopersicon esculentum in Jiangsu Province in China

Plant Disease ◽  
2018 ◽  
Vol 102 (2) ◽  
pp. 462-462 ◽  
Author(s):  
F. Zhu ◽  
Y.-P. Che ◽  
K. Qian ◽  
Y.-K. Zhou ◽  
Y.-J. Xu ◽  
...  
Keyword(s):  
Lycopersicon Esculentum ◽  
Jiangsu Province ◽  
Mottle Virus ◽  
First Report ◽  
Chilli Veinal Mottle Virus

scholarly journals First report of black raspberry necrosis virus and raspberry leaf mottle virus in Bosnia and Herzegovina

2021 ◽  
Author(s):  
Mariana Radulović ◽  
Irena Mavrič-Pleško ◽  
Francois Maclot ◽  
Duska Delić ◽  
Sebastien Massart
Keyword(s):  
Bosnia And Herzegovina ◽  
Mottle Virus ◽  
Black Raspberry ◽  
Necrosis Virus ◽  
First Report

scholarly journals First Report of Tomato chlorotic spot virus in the Non-Solanaceous Weeds Erect Spiderling (Boerhavia erecta), Asian Spiderflower (Cleome viscosa), and Sweet Chili Pepper (Capsicum chinense) in Puerto Rico

2017 ◽  
Vol 18 (1) ◽  
pp. 17-18 ◽  
Author(s):  
Consuelo Estévez de Jensen ◽  
Ismael E. Badillo-Vargas ◽  
Galen Frantz ◽  
H. Charles Mellinger ◽  
William W. Turechek ◽  
...  
Keyword(s):  
Puerto Rico ◽  
Chili Pepper ◽  
Bell Pepper ◽  
Capsicum Chinense ◽  
Spot Virus ◽  
First Report ◽  
Chlorotic Spot ◽  
New Hosts ◽  
Cleome Viscosa

Tomato chlorotic spot virus (TCSV) has recently been detected in tomato, bell pepper, jimsonweed, and lettuce in Puerto Rico. Observations of weeds and additional crops in 2015 and 2016 revealed TCSV-like symptoms. Testing of these symptomatic plants identified three new hosts of TCSV in Puerto Rico: erect spiderling (Boerhavia erecta); Asian spiderflower (Cleome viscosa); and sweet chili pepper (Capsicum chinense).

scholarly journals Pepper Mild Mottle Virus Coat Protein Alone Can Elicit the Capsicum spp. L3 Gene-Mediated Resistance

1998 ◽  
Vol 11 (12) ◽  
pp. 1253-1257 ◽  
Author(s):  
P. Gilardi ◽  
I. García-Luque ◽  
M. T. Serra
Keyword(s):  
Coat Protein ◽  
Resistance Gene ◽  
Protein Gene ◽  
Expression System ◽  
Potato Virus X ◽  
Mottle Virus ◽  
Capsicum Chinense ◽  
Pepper Mild Mottle Virus ◽  
Capsicum Spp ◽  
Virus Coat

The pepper mild mottle virus (PMMoV-S) (an L3 hypersensitive response [HR]-inducer strain) coat protein was expressed in Capsicum chinense (L3L3) plants with the heterologous potato virus X (PVX)-based expression system. The chimeric virus was localized in the inoculated leaves and induced the HR, thus indicating that the tobamoviral sequences that affect induction of the HR conferred by the L3 resistance gene reside in the coat protein gene. Furthermore, transient expression of the PMMoV-S coat protein in C. chinense leaves by biolistic co-bombardment with a plasmid expressing the β-glucuronidase (GUS) gene leads to the induction of cell death and expression of host defense genes. Thus, the coat protein of PMMoV-S is the elicitor of the Capsicum spp. L3 resistance gene-mediated HR.

scholarly journals First Report of Peanut Mottle Virus Infecting Peanut in Northeast China

Plant Disease ◽  
2019 ◽  
Vol 103 (2) ◽  
pp. 378
Author(s):  
M. N. An ◽  
R. Li ◽  
W. D. Gao ◽  
X. Y. Bi ◽  
Y. Liang ◽  
...  
Keyword(s):  
Northeast China ◽  
Mottle Virus ◽  
First Report

scholarly journals First report of canna yellow mottle virus in China

2018 ◽  
Vol 101 (1) ◽  
pp. 193-193
Author(s):  
Jianguo Shen ◽  
Fangluan Gao ◽  
Xihong Chen ◽  
Shouling Chen ◽  
Weimin Li
Keyword(s):  
Mottle Virus ◽  
First Report ◽  
Yellow Mottle

scholarly journals First Report of Cherry virus A in Sweet Cherry Trees in China

Plant Disease ◽  
2009 ◽  
Vol 93 (4) ◽  
pp. 425-425 ◽  
Author(s):  
W.-L. Rao ◽  
Z.-K. Zhang ◽  
R. Li
Keyword(s):  
Sweet Cherry ◽  
Community Gardens ◽  
Mottle Virus ◽  
Replicase Gene ◽  
Rt Pcr ◽  
First Report ◽  
Type Isolate ◽  
The Family ◽  
Flowering Cherry ◽  
Cherry Trees

Plants in the genus Prunus of the family Rosaceae are important fruit and ornamental trees in China. In June of 2007, sweet cherry (Prunus avium) trees with mottling and mosaic symptoms were observed in a private garden near Kunming, Yunnan Province. Twenty-four samples, six each from sweet cherry, sour cherry (P. cerasus), flowering cherry (P. serrulata), and peach (P. persica) were collected from trees in private and community gardens in the area. The peach and sour and flowering cherry trees did not show any symptoms. Total nucleic acids were extracted using a cetyltrimethylammoniumbromide (CTAB) extraction method, and the extracts were tested for the following eight viruses by reverse transcription (RT)-PCR: American plum line pattern virus, Apple chlorotic leaf spot virus, Cherry green ring mottle virus, Cherry necrotic rusty mottle virus, Cherry virus A (CVA), Little cherry virus 1, Prune dwarf virus, and Prunus necrotic ringspot virus. Only CVA was detected in two symptomatic sweet cherry trees by RT-PCR with forward (5′-GTGGCATTCAACTAGCACCTAT-3′) and reverse (5′-TCAGCTGCCTCAGCTTGGC-3′) primers specific to an 873-bp fragment of the CVA replicase gene (2). The CVA infection of the two trees was confirmed by RT-PCR using primers CVA-7097U and CVA-7383L that amplified a 287-bp fragment from the 3′-untranslated region (UTR) of the virus (1). Amplicons from both amplifications were cloned and sequenced. Analysis of the predicted amino acid sequences of the 873-bp fragments (GenBank Accession Nos. EU862278 and EU862279) showed that they were 98% identical with each other and 97 to 98% with the type isolate of CVA from Germany (GenBank Accession No. NC_003689). The 286-bp sequences of the 3′-UTR (GenBank Accession Nos. FJ608982 and FJ608983) were 93% identical with each other and 93 to 98% with the type isolate. The sequence indicated that the three isolates were very similar and should be considered to be the same strain. CVA is a member of the genus Capillovirus in the family Flexiviridae and has been previously reported in Europe, North America, and Japan. The contribution of CVA to the symptoms observed and its distribution in China remain to be evaluated. To our knowledge, this is the first report of CVA in sweet cherry in China. References: (1) M. Isogai et al. J. Gen. Plant Pathol. 70:288. (2) W. Jelkmann. J. Gen. Virol. 76:2015, 1995.

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