Experimental Exposures of Boreal Toads (Bufo boreas) to a Pathogenic Chytrid Fungus (Batrachochytrium dendrobatidis)

EcoHealth ◽  
2006 ◽  
Vol 3 (1) ◽  
pp. 5-21 ◽  
Author(s):  
Cynthia Carey ◽  
Judsen E. Bruzgul ◽  
Lauren J. Livo ◽  
Margie L. Walling ◽  
Kristin A. Kuehl ◽  
...  
Keyword(s):  
Batrachochytrium Dendrobatidis ◽  
Chytrid Fungus ◽  
Bufo Boreas ◽  
Boreal Toads

scholarly journals Inhibition of Batrachochytrium dendrobatidis Caused by Bacteria Isolated from the Skin of Boreal Toads, Anaxyrus (Bufo) boreas boreas, from Grand Teton National Park, Wyoming, USA

2014 ◽  
Vol 7 ◽  
pp. MBI.S13639 ◽  
Author(s):  
Shawna T. Park ◽  
Amanda M. Collingwood ◽  
Sophie St-Hilaire ◽  
Peter P. Sheridan
Keyword(s):  
National Park ◽  
Batrachochytrium Dendrobatidis ◽  
Chytrid Fungus ◽  
Bacterial Isolates ◽  
Skin Microbiota ◽  
Amphibian Species ◽  
Bufo Boreas ◽  
Grand Teton National Park ◽  
Boreal Toads

The chytrid fungus Batrachochytrium dendrobatidis is a significant cause of the worldwide decline in amphibian populations; however, various amphibian species are capable of coexisting with B. dendrobatidis. Among them are boreal toads ( Anaxyrus ( Bufo) boreas boreas) located in Grand Teton National Park (GTNP) in Wyoming, USA. The purpose of this study was to identify cultivable bacterial isolates from the skin microbiota of boreal toads from GTNP and determine if they were capable of inhibiting B. dendrobatidis in vitro, and therefore might be a factor in the toad's coexistence with this pathogen. Isolates from 6 of 21 genera tested were found to inhibit the growth of B. dendrobatidis. These bacteria represent diverse lineages such as the Gammaproteobacteria, the Betaproteobacteria, and the Bacteroidetes/Chlorobium groups. We propose that these bacteria compete via microbial antagonism with B. dendrobatidis.

scholarly journals The Effect of Aquatic and Terrestrial Environmental Factors on the Interaction Between Grand Teton Boreal Toads and a Lethal Fungal Pathogen

2008 ◽  
Vol 31 ◽  
pp. 125-131
Author(s):  
Peter Murphy ◽  
Sophie St-Hilaire ◽  
Charles Peterson
Keyword(s):  
Fungal Pathogen ◽  
Batrachochytrium Dendrobatidis ◽  
Chytrid Fungus ◽  
Population Declines ◽  
Amphibian Skin ◽  
Amphibian Species ◽  
American Bullfrog ◽  
The North ◽  
S 100 ◽  
Boreal Toads

Batrachochytrium dendrobatidis (Bd), the chytrid fungus which infects keratinized amphibian skin and causes the lethal disease chytridiomycosis, has been linked to population declines and extinctions worldwide (Lips et al. 2006). Amphibians infected with Bd may suffer a variety of outcomes. Individuals of some species have been killed by :S 100 Bd zoospores, while other species, such as the North American bullfrog Rana catesbiana, are highly resistant (Daszak et al. 2004). Within an amphibian species, populations may also respond differently to Bd, with some declining et al. remaining stable (Kriger and Hero 2006). Divergent outcomes among species and populations with respect to Bd may arise from at least three factors, or their interaction.

Evaluation of the Skin Peptide Defenses of the Oregon Spotted Frog Rana pretiosa Against Infection by the Chytrid Fungus Batrachochytrium dendrobatidis

2013 ◽  
Vol 39 (6) ◽  
pp. 797-805 ◽  
Author(s):  
J. Michael Conlon ◽  
Laura K. Reinert ◽  
Milena Mechkarska ◽  
Manju Prajeep ◽  
Mohammed A. Meetani ◽  
...  
Keyword(s):  
Batrachochytrium Dendrobatidis ◽  
Chytrid Fungus ◽  
Rana Pretiosa

scholarly journals Is overwintering mortality driving enigmatic declines? Evaluating the impacts of trematodes and the amphibian chytrid fungus on an anuran from hatching through overwintering

PLoS ONE ◽  
2022 ◽  
Vol 17 (1) ◽  
pp. e0262561
Author(s):  
Olivia Wetsch ◽  
Miranda Strasburg ◽  
Jessica McQuigg ◽  
Michelle D. Boone
Keyword(s):  
Batrachochytrium Dendrobatidis ◽  
Species Conservation ◽  
Emerging Infectious Diseases ◽  
The Novel ◽  
Chytrid Fungus ◽  
Growth And Survival ◽  
Critical Life Events ◽  
Overwintering Mortality ◽  
Amphibian Chytrid Fungus

Emerging infectious diseases are increasing globally and are an additional challenge to species dealing with native parasites and pathogens. Therefore, understanding the combined effects of infectious agents on hosts is important for species’ conservation and population management. Amphibians are hosts to many parasites and pathogens, including endemic trematode flatworms (e.g., Echinostoma spp.) and the novel pathogenic amphibian chytrid fungus (Batrachochytrium dendrobatidis [Bd]). Our study examined how exposure to trematodes during larval development influenced the consequences of Bd pathogen exposure through critical life events. We found that prior exposure to trematode parasites negatively impacted metamorphosis but did not influence the effect of Bd infection on terrestrial growth and survival. Bd infection alone, however, resulted in significant mortality during overwintering—an annual occurrence for most temperate amphibians. The results of our study indicated overwintering mortality from Bd could provide an explanation for enigmatic declines and highlights the importance of examining the long-term consequences of novel parasite exposure.

scholarly journals Establishement of a national monitoring program based on environmental DNA for amphibians and the chytrid fungus Batrachochytrium dendrobatidis     

2021 ◽  
Vol 4 ◽  
Author(s):  
Omneya Osman ◽  
Johan Andersson ◽  
Tomas Larsson ◽  
Mats Töpel ◽  
Alexander Eiler
Keyword(s):  
Batrachochytrium Dendrobatidis ◽  
National Level ◽  
Monitoring Program ◽  
12S Rrna ◽  
Rrna Gene ◽  
Chytrid Fungus ◽  
Rana Arvalis ◽  
Amphibian Species ◽  
National Monitoring ◽  
Wide Range

National monitoring programs provide the basis for evaluating the integrity of ecosystems, their responses to disturbances, and the success of actions taken to conserve or recover biodiversity. In this study, we successfully established a national program for the invasive chytrid fungus Batrachochytrium dendrobatidis (Bd) based on dual TaqMan assays. Amphibian diversity based on metabarcoding of the mitochondrial 12S rRNA gene was also performed. Assays were optimized for sensitive detection of target species from a wide range of amphibian ponds with variable potential of inhibitions for eDNA based detection. An amphibian mock community of 5 species was used to validate the metabarcoding approach while internal standards were used in the case of the dual TaqMan assays. First sampling of over 170 ponds in Norway resulted in Bd detection in 12 environmental samples and one swab sample taken over multiple years indicating the establishment of Bd in Norway. Five amphibian species Bufo bufo, Lissotriton vulgaris, Triturus cristatus, Rana arvalis and Rana temporaria as predicted from data in long-term citizen science reporting systems were widely detected in the collected eDNA samples. Our large scale-monitoring program indicates a low risk of a Bd outbreak and amphibian decline caused by chytridiomycosis but continued monitoring is recommended in the future. These findings indicate that eDNA is an effective method to detect invasive species, and to monitor endangered amphibian species. Still, several shortcomings (such as PCR inhibitors and sample volume) were identified that need to be addressed to improve eDNA-based monitoring at the national level.

Non-detection of mycoviruses in amphibian chytrid fungus (Batrachochytrium dendrobatidis) from Australia

2021 ◽  
Author(s):  
Rebecca J. Webb ◽  
Alexandra A. Roberts ◽  
Stephen Wylie ◽  
Tiffany Kosch ◽  
Luís Felipe Toledo ◽  
...  
Keyword(s):  
Batrachochytrium Dendrobatidis ◽  
Chytrid Fungus ◽  
Amphibian Chytrid Fungus

scholarly journals Sensitivity of histology for the detection of the amphibian chytrid fungus Batrachochytrium dendrobatidis

2019 ◽  
Vol 31 (2) ◽  
pp. 246-249 ◽  
Author(s):  
Claudio Borteiro ◽  
Francisco Kolenc ◽  
José Manuel Verdes ◽  
Claudio Martínez Debat ◽  
Martín Ubilla
Keyword(s):  
Skin Disease ◽  
Batrachochytrium Dendrobatidis ◽  
Pathogenic Fungus ◽  
Chytrid Fungus ◽  
Clinically Normal ◽  
Frog Species ◽  
Amphibian Chytrid Fungus

Histology is often underappreciated for the detection of the amphibian pathogenic fungus Batrachochytrium dendrobatidis, the cause of the potentially lethal skin disease chytridiomycosis. We evaluated the sensitivity of histology to detect chytrids in 20 wild specimens of 2 frog species from Uruguay that were clinically normal, but confirmed by PCR to be infected by B. dendrobatidis. We detected maturing and sporulated sporangia in 15 of 20 (75%) frogs, which is more sensitive than previously reported for histology. The effort needed to identify chytrids in histologic skin sections of Physalaemus henselii and Pleurodema bibroni required examination of 3.2 and 8.7 mm of skin sections for each frog species, respectively.

scholarly journals Chytrid fungus Batrachochytrium dendrobatidis has nonamphibian hosts and releases chemicals that cause pathology in the absence of infection

2012 ◽  
Vol 110 (1) ◽  
pp. 210-215 ◽  
Author(s):  
T. A. McMahon ◽  
L. A. Brannelly ◽  
M. W. H. Chatfield ◽  
P. T. J. Johnson ◽  
M. B. Joseph ◽  
...  
Keyword(s):  
Batrachochytrium Dendrobatidis ◽  
Chytrid Fungus
Sign in / Sign up

Export Citation Format

Share Document