Tumor Ablation Enhancement by Combining Radiofrequency Ablation and Irreversible Electroporation: An In Vitro 3D Tumor Study

2018 ◽  
Vol 47 (3) ◽  
pp. 694-705 ◽  
Author(s):  
Bing Zhang ◽  
Yongji Yang ◽  
Lujia Ding ◽  
Michael A. J. Moser ◽  
Edwin M. Zhang ◽  
...  
Keyword(s):  
Radiofrequency Ablation ◽  
Irreversible Electroporation ◽  
Tumor Ablation ◽  
Tumor Study

scholarly journals Tumor Ablation with Irreversible Electroporation

PLoS ONE ◽  
2007 ◽  
Vol 2 (11) ◽  
pp. e1135 ◽  
Author(s):  
Bassim Al-Sakere ◽  
Franck André ◽  
Claire Bernat ◽  
Elisabeth Connault ◽  
Paule Opolon ◽  
...  
Keyword(s):  
Irreversible Electroporation ◽  
Tumor Ablation

Silencing of lncRNA MEG8 Represses the Viability, Migration, and Invasion of Wilms’ Tumor Cells through Mediating miR-23a-3p/CRK Axis

2021 ◽  
pp. 1-13
Author(s):  
Jing Shen ◽  
Qiang Shu
Keyword(s):  
Cell Viability ◽  
Wilms Tumor ◽  
Luciferase Reporter ◽  
Migration And Invasion ◽  
Transwell Assay ◽  
Reaction Cell ◽  
Tumor Study ◽  
Polymerase Chain ◽  
Dual Luciferase Reporter Assay

<b><i>Purpose:</i></b> Compelling evidence has unveiled the importance of long noncoding RNAs (lncRNAs) in malignant behavior of Wilms’ tumor (WT). Hereon, we intend to assess the function and associated molecular mechanism of lncRNA maternally expressed gene 8 (MEG8) in WT cells. <b><i>Methods:</i></b> Expression levels of MEG8, miR-23a-3p, and CT10 regulator of kinase (CRK) were determined by quantitative real-time polymerase chain reaction. Cell viability was assessed by MTT assay. Besides, wound healing assay and transwell assay were applied to examine abilities of cell migration and invasion, respectively. Dual-luciferase reporter assay was employed to test the interplay among MEG8, miR-23a-3p, and CRK. Western blot was used to detect relative protein expression of CRK. <b><i>Results:</i></b> MEG8 and CRK expression was elevated, while miR-23a-3p expression was decreased in WT tissues and cells. The histologic type, lymphatic metastasis, and National Wilms Tumor Study (NWTS) stage were associated with the expression of MEG8, miR-23a-3p, and CRK in WT patients. MEG8 knockdown or miR-23a-3p overexpression restrained WT cells in cell viability, migration, and invasiveness in vitro. As to mechanism exploration, MEG8 could directly bind to miR-23a-3p and then miR-23a-3p targeted CRK. MEG8 was inversely correlated with miR-23a-3p and positively correlated with CRK in WT tissues. Meantime, miR-23a-3p was inversely correlated with CRK in WT tissues. Additionally, MEG8 knockdown-mediated suppressive impacts on cell viability, migration, and invasiveness were reversed by overexpression of CRK or repression of miR-23a-3p in WT cells. <b><i>Conclusions:</i></b> The cell viability, migration, and invasiveness of WT cells were repressed by MEG8 knockdown via targeting the miR-23a-3p/CRK axis.

Assessment of Various Types of US Findings after Irreversible Electroporation in Porcine Liver: Comparison with Radiofrequency Ablation

2015 ◽  
Vol 26 (2) ◽  
pp. 279-287.e3 ◽  
Author(s):  
Katsutoshi Sugimoto ◽  
Fuminori Moriyasu ◽  
Yoshiyuki Kobayashi ◽  
Kazuhiko Kasuya ◽  
Yuichi Nagakawa ◽  
...  
Keyword(s):  
Radiofrequency Ablation ◽  
Irreversible Electroporation ◽  
Porcine Liver ◽  
Us Findings

Effects of Blood Flow on Radiofrequency Ablation of Tumors: Finite Elements and In Vitro Models

2003 ◽  
Author(s):  
Juan R. Cebral ◽  
Orlando Soto ◽  
Robert J. Lutz ◽  
Bradford J. Wood
Keyword(s):  
Blood Flow ◽  
Radiofrequency Ablation ◽  
Saline Solution ◽  
Numerical Models ◽  
In Vitro Models ◽  
Collective Effects ◽  
Ablation Lesion ◽  
Thermal Flow ◽  
Flow Process

The efficacy of radiofrequency ablation (RFA) treatments depends on the ability to ablate tumors completely while minimizing the damage to healthy tissue. Tissue cooling due to blood flow is an important factor affecting the size and shape of the ablation lesion. In this paper a new methodology for finite element modeling of the coupled electrical-thermal-flow process during RFA is presented. Our formulation treats heat losses due to blood flow explicitly rather than approximating the collective effects of blood vessles as a heat sink. Numerical models were compared to in vitro models using egg whites to simulate human tissue and a straight cylinder filled with a saline solution to simulate blood. Asymmetric burns were obtained close to the simulated blood vessels. Numerical results closely match the in vitro models.

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