Strain differentiation of airborne opportunistic microorganisms within a municipal landfill area as assessed by PCR MP method

Aerobiologia ◽  
2016 ◽  
Vol 32 (3) ◽  
pp. 499-511 ◽  
Author(s):  
Krzysztof Frączek ◽  
Jacek Kozdrój
Keyword(s):  
Strain Differentiation ◽  
Municipal Landfill ◽  
Mp Method ◽  
Pcr Mp

scholarly journals PCR MP Method for Differentiation of Clavibacter michiganensis Subsp. sepedonicus Isolates

2019 ◽  
Vol 96 (6) ◽  
pp. 588-604 ◽  
Author(s):  
Agnieszka Węgierek-Maciejewska ◽  
Aleksander Masny ◽  
Tomasz Wołkowicz ◽  
Agata Jagiełło ◽  
Edward Arseniuk
Keyword(s):  
Restriction Enzymes ◽  
Epidemiological Studies ◽  
Diagnostic Methods ◽  
Molecular Diagnostic ◽  
Strain Identification ◽  
Published Data ◽  
Clavibacter Michiganensis ◽  
Electrophoretic Patterns ◽  
Mp Method ◽  
Pcr Mp

AbstractThe published data indicate that the bacterium Clavibacter michiganensis subsp. sepedonicus (Cms) shows a notably low degree of intraspecific variation, and most of the molecular diagnostic methods could differentiate among subspecies groups but lacked the resolution to detect genetic diversity within subspecies. The present study describes effective differentiation of 50 isolates of C. michiganensis subsp. sepedonicus by the PCR MP method using five restriction enzymes (ApaI, PstI, BamHI, XmaI, HindIII). By analysing the electrophoretic patterns obtained by the PCR MP method performed with the applied restriction enzymes, each of the 50 tested isolates could be identified and distinguished from the remaining isolates. The PCR MP method of bacterial strain identification can be used in epidemiological studies, particularly for excluding the isolates of C. michiganensis subsp. sepedonicus as a source of primary infection.

P1849 Molecular typing of clinical isolates of Escherichia coli by a new PCR MP method

2007 ◽  
Vol 29 ◽  
pp. S529
Author(s):  
B. Krawczyk ◽  
A. Sledzinska ◽  
A. Samet ◽  
M. Bronk ◽  
J. Komarnicka ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Molecular Typing ◽  
Clinical Isolates ◽  
Mp Method ◽  
Pcr Mp

scholarly journals Application of Microsatellite-Primed PCR (MSP-PCR) and PCR Melting Profile (PCR-MP) Method for Intraspecies Differentiation of Dermatophytes

2014 ◽  
Vol 63 (3) ◽  
pp. 283-290 ◽  
Author(s):  
ANITA CIESIELSKA ◽  
MAGDALENA KOZŁOWSKA ◽  
MAREK GADZALSKI ◽  
MARIUSZ WOREK ◽  
ADAM JAWORSKI ◽  
...  
Keyword(s):  
Trichophyton Rubrum ◽  
Microsporum Canis ◽  
Melting Profile ◽  
Trichophyton Interdigitale ◽  
Mp Method ◽  
Method Analysis ◽  
Pcr Mp

In this study, two PCR-based methods (MSP-PCR and PCR-MP) were compared for their abilities to identify intraspecies variations of 23 isolates of Trichophyton rubrum, 78 isolates of Trichophyton interdigitale and 22 isolates of Microsporum canis, obtained mainly from patients in Lódź city. The results allowed to distinguish four types (containing two subtypes) characteristic for T. interdigitale and three types characteristic for T. rubrum using PCR-MP method. Analysis conducted using MSP-PCR with (GACA)4 primer revealed four types for T. rubrum and three types (containing one subtype) for T. interdigitale and with (GTG), primer showed two types (containing one subtype) for T. rubrum and six types (containing one subtype) for T. interdigitale. No differentiation was observed for the M. canis isolates with either method.

Preremedial assessment of the municipal landfill pollution impact on soil and shallow groundwater in Subotica, Serbia

2018 ◽  
Vol 615 ◽  
pp. 1341-1354 ◽  
Author(s):  
Dejan Krčmar ◽  
Slaven Tenodi ◽  
Nenad Grba ◽  
Djurdja Kerkez ◽  
Malcolm Watson ◽  
...  
Keyword(s):  
Shallow Groundwater ◽  
Municipal Landfill ◽  
Pollution Impact

Stripping Technology of Beach Bar Sandstone Strong Reflection Cover Based on MP Method

2014 ◽  
Author(s):  
Liu Peijin* ◽  
Huang Guangtan ◽  
Zhang Junhua ◽  
Ban Li ◽  
Liu Lei
Keyword(s):  
Strong Reflection ◽  
Mp Method ◽  
Beach Bar

Effect of advanced oxidation processes on the toxicity of municipal landfill leachates

2004 ◽  
Vol 49 (4) ◽  
pp. 273-277 ◽  
Author(s):  
B. Slomczynska ◽  
J. Wasowski ◽  
T. Slomczynski
Keyword(s):  
Organic Compounds ◽  
Advanced Oxidation Processes ◽  
Vibrio Fischeri ◽  
Advanced Oxidation ◽  
Optimal Conditions ◽  
Toxicity Evaluation ◽  
Landfill Leachates ◽  
Oxidation Processes ◽  
Municipal Landfill ◽  
Pre Treatment

The aim of the present study was to assess the effect of advanced oxidation processes (AOPs) (oxidation ozone and peroxide/ozone) on the toxicity of leachates from municipal landfill for Warsaw, Poland, using a battery of tests. AOPs used to pre-treat leachates were carried out in laboratory conditions after their coagulation with the use of FeCl3. The effects of the pre-treatment of leachates using the method of coagulation with FeCl3 depended on the concentration of organic compounds and with optimal conditions of the process ranged from 40 to 70%. Further pre-treatment of the leachates after coagulation, involving the use of oxidation with O3 and H2O2/O3, did not cause significant decrease of leachate toxicity. The data of this study demonstrated the usefulness of the battery of tests using Daphnia magna, Artemia franciscana, Scenedesmus quadricauda and Vibrio fischeri for the toxicity evaluation of raw and pre-treated leachates.

Sign in / Sign up

Export Citation Format

Share Document