scholarly journals Application of Microsatellite-Primed PCR (MSP-PCR) and PCR Melting Profile (PCR-MP) Method for Intraspecies Differentiation of Dermatophytes

2014 ◽  
Vol 63 (3) ◽  
pp. 283-290 ◽  
Author(s):  
ANITA CIESIELSKA ◽  
MAGDALENA KOZŁOWSKA ◽  
MAREK GADZALSKI ◽  
MARIUSZ WOREK ◽  
ADAM JAWORSKI ◽  
...  
Keyword(s):  
Trichophyton Rubrum ◽  
Microsporum Canis ◽  
Melting Profile ◽  
Trichophyton Interdigitale ◽  
Mp Method ◽  
Method Analysis ◽  
Pcr Mp

In this study, two PCR-based methods (MSP-PCR and PCR-MP) were compared for their abilities to identify intraspecies variations of 23 isolates of Trichophyton rubrum, 78 isolates of Trichophyton interdigitale and 22 isolates of Microsporum canis, obtained mainly from patients in Lódź city. The results allowed to distinguish four types (containing two subtypes) characteristic for T. interdigitale and three types characteristic for T. rubrum using PCR-MP method. Analysis conducted using MSP-PCR with (GACA)4 primer revealed four types for T. rubrum and three types (containing one subtype) for T. interdigitale and with (GTG), primer showed two types (containing one subtype) for T. rubrum and six types (containing one subtype) for T. interdigitale. No differentiation was observed for the M. canis isolates with either method.

scholarly journals Evaluation of a PCR melting profile method for intraspecies differentiation of Trichophyton rubrum and Trichophyton interdigitale

2010 ◽  
Vol 59 (2) ◽  
pp. 185-192 ◽  
Author(s):  
Justyna Leibner-Ciszak ◽  
Anita Dobrowolska ◽  
Beata Krawczyk ◽  
Aleksandra Kaszuba ◽  
Paweł Stączek
Keyword(s):  
Trichophyton Rubrum ◽  
Pathogen Transmission ◽  
Microsporum Canis ◽  
Melting Profile ◽  
Melting Temperatures ◽  
Trichophyton Interdigitale ◽  
Dna Restriction Fragments ◽  
Dna Restriction ◽  
The Difference ◽  
Pcr Mp

In order to identify the source of infections caused by dermatophytes, as well as the pathogen transmission pathway, there is a need to determine methods that allow detailed genetic differentiation of the strains within the dermatophyte genera. In this work, a PCR melting profile (PCR-MP) technique based on the ligation of adaptors and the difference in melting temperatures of DNA restriction fragments was used for the first time for intraspecies genotyping of dermatophytes. Clinical isolates and reference strains of dermatophytes isolated from skin, scalp, toenails and fingernails were used for this study. PCR-MP and random amplification of polymorphic DNA (RAPD) were used to type 11 isolates of Trichophyton rubrum, 40 isolates of Trichophyton interdigitale and 14 isolates of Microsporum canis. The results distinguished five types (containing one subtype) characteristic for T. rubrum and seven types characteristic for T. interdigitale using the PCR-MP technique. Analysis conducted using RAPD revealed five types for T. rubrum and four types for T. interdigitale isolates. No differentiation was observed for the M. canis isolates with either method. These results demonstrate that PCR-MP is a reliable method for the differentiation of T. rubrum and T. interdigitale strains and yields a discriminatory power that is at least equal to that of RAPD.

scholarly journals Superficial mycosis profile in the Tertiary Hospital Dr. Moh. Hoesin Palembang: retrospective research

2020 ◽  
Vol 1 (1) ◽  
pp. 31-40
Author(s):  
Desi Harnis ◽  
Rusmawardiana ◽  
, Fifa Argentina
Keyword(s):  
Diabetes Mellitus ◽  
High Temperature ◽  
Medical Records ◽  
Trichophyton Rubrum ◽  
Tertiary Hospital ◽  
Microsporum Canis ◽  
Superficial Mycoses ◽  
Superficial Mycosis ◽  
Temperature And Humidity ◽  
Unskilled Workers

Background Superficial mycoses is a fungal infection of the skin, nails and hair thatcaused by dematophytes, yeast and mold. Superficial mycoses infections are commonlyfound in high temperature and humidity area such as Indonesia. Palembang is one ofregions in Indonesia has a high temperature and humidity, considered the incidenceof this disease is high. A retrospective study of new cases of superficial mycoses, datataken from medical records and register book at Policlinic of Dermatology andVenereology Dr. Moh. Hoesin Palembang during 5 years from January 2014-December2018. There were 1,236 (17.1%) new cases of superficial mycoses. Incidence ofsuperficial mycoses varies with range 6.9%-23%. The most common superficialmycoses is dermatophytes (38,3%). Microsporum canis, Trichophyton rubrum danTricophyton hmentagrophytes are frequent isolates in this study. The most age groupis 36-45 years (17.6%) with male more than female. Superficial mycoses are often foundin students (26.9%), followed by unskilled workers (20.8%). Of the 1,236 new cases,125 patients had comorbidities, such as malignancy 21 (16.8%) patients,cerebrovascular disease and diabetes mellitus each 16 patients (7.4%). Inguinal andabdomen are the most commonly infected regions. 656 (53.1%) patients was giventopical antifungal. The most common topical antifungal was ketoconazole 2% cream(62%) and systemic antifungal was itraconazole (55,3%).Conclusion: Cases ofsuperficial mycoses, especially dermatophytosis and Malassezia were still commonlyfound, especially in Palembang.

scholarly journals Dermatofitosis humana en una comunidad con desigualdad social, Ayacucho 2018

2019 ◽  
Vol 27 (1) ◽  
Author(s):  
Serapio Romero Gavilán
Keyword(s):  
Trichophyton Rubrum ◽  
Trichophyton Mentagrophytes ◽  
Microsporum Canis ◽  
Microsporum Gypseum

Trabajo de investigación desarrollado con el objetivo de conocer la frecuencia de la dermatofitosis humana en una comunidad con desigualdad social. Hipótesis: la dermatofitosis humana es una afección fúngica muy frecuente en comunidades con desigualdad social. Zona de estudio: comunidad con desigualdad social periurbana de la ciudad de Ayacucho. Tipo de estudio: no experimental. Diseño de estudio: descriptivo-transversal. Muestra: no probabilística, individuos con signos de afecciones compatibles a micosis superficial. Metodología: muestras de escamas de piel, pelos, fragmentos de uñas de pies y manos, escamas de planta, espacios interdigitales y otras partes de cuerpo, fueron tomadas con una hoja de bisturí de filo romo o con el borde de un portaobjetos previa desinfección con alcohol al 70% y colocadas en sobres de papel oscuro etiquetados, en el laboratorio de Epidemiología y Micología de la Universidad Nacional de San Cristóbal de Huamanga, se realizó la observación directa con KOH al 20% y la siembra en placas Petri conteniendo Agar Sabouraud y Agar selectivo para hongos patógenos, después de una incubación a 25ºC por hasta 14 días; las colonias coincidentes con dermatofitos fueron observados al microscopio para identificarlos. Resultados: se ha encontrado que 85/153 (55,5%) presentaron diversas formas de dermatofitosis, no se observó preferencia de la dermatofitosis humana con relación al género (p> 0,05), los factores asociados a la dermatofitosis determinados estadísticamente (p< 0,05) fueron la higiene, el piso de la vivienda y la crianza de animales, se han identificado las especies de Trichophyton rubrum, Trichophyton mentagrophytes, Microsporum canis y Microsporum gypseum.

Assembly and analysis of the whole genome of Arthroderma uncinatum strain T10, compared with Microsporum canis and Trichophyton rubrum

Mycoses ◽  
2020 ◽  
Vol 63 (7) ◽  
pp. 683-693
Author(s):  
Hailin Zheng ◽  
Oliver Blechert ◽  
Huan Mei ◽  
Liyu Ge ◽  
Jia Liu ◽  
...  
Keyword(s):  
Trichophyton Rubrum ◽  
Microsporum Canis ◽  
Whole Genome

Contact-free inactivation of Trichophyton rubrum and Microsporum canis by cold atmospheric plasma treatment

2013 ◽  
Vol 8 (9) ◽  
pp. 1097-1106 ◽  
Author(s):  
Julia Heinlin ◽  
Tim Maisch ◽  
Julia L Zimmermann ◽  
Tetsuji Shimizu ◽  
Thomas Holzmann ◽  
...  
Keyword(s):  
Plasma Treatment ◽  
Trichophyton Rubrum ◽  
Atmospheric Plasma ◽  
Microsporum Canis ◽  
Cold Atmospheric Plasma ◽  
Contact Free

scholarly journals In vitro evaluation of the antifungal activity of Marco (Ambrosia arborescens Mill.) and Matico (Aristeguietia glutinosa Lam.) on the pathogenic fungi that cause dermatomycosis (ringworm)

F1000Research ◽  
2018 ◽  
Vol 7 ◽  
pp. 559
Author(s):  
Tatiana de los Ángeles Mosquera Tayupanta ◽  
Sandra Elizabeth Ayala Valarezo ◽  
Tatiana Alexandra Vasquez Villareal ◽  
María Belén Montaluisa Álvarez
Keyword(s):  
Candida Albicans ◽  
Antifungal Activity ◽  
Trichophyton Rubrum ◽  
Therapeutic Potential ◽  
Pathogenic Fungi ◽  
Trichophyton Mentagrophytes ◽  
Microsporum Canis ◽  
The One ◽  
One Way Anova

Background: Currently, there is a trend towards using natural and ethnopharmacological species with therapeutic potential. This investigation evaluated the antifungal activity of two species in the Ecuadorian Andes, which are used in treating dermatomycosis: Ambrosia arborescens Mill. (Marco) and Aristeguietia glutinosa Lam. (Matico). Methods: We worked with seven concentrations (100 to 700ppm) of Ambrosia arborescens Mill. extract and ten concentrations (0.5 to 5%) of essential oil (EO) of Aristeguietia glutinosa Lam. on Trichophyton mentagrophytes ATCC 9533, Trichophyton rubrum ATCC 28188, Microsporum canis ATCC 36299 and Candida albicans ATCC 10231. The methodology used was a modified version of the Kirby-Bauer method, using diffusion in agar wells. Results: The Tukey test, after the one-way Anova, determined effective concentrations of EO: 5% for Trichophyton mentagrophytes, 4.5% for Trichophyton rubrum, 5% for Microsporum canis and 2% for Candida albicans. In the extracts, the concentration of 700ppm was used for Trichophyton mentagrophytes, Trichophyton rubrum, and 600ppm for Microsporum canis and Candida albicans. Conclusions: The evaluation of the antifungal activity of the Ambrosia arborescens extract showed inhibition in the studied dermatophytes in each one of the planted concentrations (100 to 700ppm). The evaluation of the antifungal activity of Aristeguietia glutinosa EO showed inhibition in the studied dermatophytes in each of the planted concentrations (0.5 to 5%).

scholarly journals PCR MP Method for Differentiation of Clavibacter michiganensis Subsp. sepedonicus Isolates

2019 ◽  
Vol 96 (6) ◽  
pp. 588-604 ◽  
Author(s):  
Agnieszka Węgierek-Maciejewska ◽  
Aleksander Masny ◽  
Tomasz Wołkowicz ◽  
Agata Jagiełło ◽  
Edward Arseniuk
Keyword(s):  
Restriction Enzymes ◽  
Epidemiological Studies ◽  
Diagnostic Methods ◽  
Molecular Diagnostic ◽  
Strain Identification ◽  
Published Data ◽  
Clavibacter Michiganensis ◽  
Electrophoretic Patterns ◽  
Mp Method ◽  
Pcr Mp

AbstractThe published data indicate that the bacterium Clavibacter michiganensis subsp. sepedonicus (Cms) shows a notably low degree of intraspecific variation, and most of the molecular diagnostic methods could differentiate among subspecies groups but lacked the resolution to detect genetic diversity within subspecies. The present study describes effective differentiation of 50 isolates of C. michiganensis subsp. sepedonicus by the PCR MP method using five restriction enzymes (ApaI, PstI, BamHI, XmaI, HindIII). By analysing the electrophoretic patterns obtained by the PCR MP method performed with the applied restriction enzymes, each of the 50 tested isolates could be identified and distinguished from the remaining isolates. The PCR MP method of bacterial strain identification can be used in epidemiological studies, particularly for excluding the isolates of C. michiganensis subsp. sepedonicus as a source of primary infection.

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