scholarly journals Molecular analysis of genes involved in chitin degradation from the chitinolytic bacterium Bacillus velezensis

2022 ◽  
Author(s):  
Dinh Minh Tran ◽  
To Uyen Huynh ◽  
Thi Huyen Nguyen ◽  
Tu Oanh Do ◽  
Quang-Vinh Nguyen ◽  
...  
Keyword(s):  
Molecular Analysis ◽  
Bacillus Velezensis ◽  
Chitin Degradation ◽  
Bacterium Bacillus

scholarly journals A Novel Rhizospheric Bacterium, Bacillus Velezensis NKMV-3 as a Biocontrol Agent against Alternaria Leaf Blight in Tomato

2021 ◽  
Author(s):  
Vignesh Murthy ◽  
VedhaHari BodethalaNarayanan ◽  
MubarakAli Davoodbasha ◽  
MadhanShankar ShankarRamakrishanan
Keyword(s):  
Early Blight ◽  
Plant Pathogens ◽  
Alternaria Solani ◽  
Inhibitory Effect ◽  
Rrna Gene ◽  
Bacillus Velezensis ◽  
Bacterium Bacillus ◽  
Blight Disease ◽  
Molecular Sequencing

Abstract A novel strain of Bacillus isolated from rhizosphere has shown to be excellent biocontrol agents against various plant pathogens. In this study, a first report of a Bacillus strain NKMV-3 which effectively controlling Alternaria solani, which cause the Early Blight disease in tomato. Based on the cultural and molecular sequencing of 16S rRNA gene sequence, the identity of the strain was confirmed as Bacillus velezensis NKMV-3. The presence of the lipopeptide which are antibiotic synthesis genes namely Iturin C, Surfactin A, Fengycin B and D were confirmed through gene amplification. In addition, lipopetides was also confirmed through liquid chromatography. The extract showed inhibitory effect against A.solani in-vitro and detached tomato leaf assays. Bacillus velezensis strain NKMV-3 based formulations may provide an effective solution in controlling early blight disease in tomato and other crops.

scholarly journals Purification and characterization of a surfactin-like biosurfactant produced by Bacillus velezensis KLP2016 and its application towards engine oil degradation

2020 ◽  
Author(s):  
Khem Raj Meena ◽  
Rajni Dhiman ◽  
Kailash Singh ◽  
Sachin Kumar ◽  
Abhishek Sharma ◽  
...  
Keyword(s):  
Oil Pollution ◽  
Luria Bertani ◽  
Size Exclusion ◽  
Engine Oil ◽  
Bacillus Velezensis ◽  
Emulsification Index ◽  
Luria Bertani Broth ◽  
Pollution Problem ◽  
Exclusion Chromatography ◽  
Bacterium Bacillus

Abstract Engine oil used in automobiles is a threat to soil and water due to recalcitrant properties of its hydrocarbons. It pollute surrounding environment which affect both flora and fauna of earth. Microbes are able to degrade hydrocarbons containing engine oil to utilize as a substrate for their growth. Our results demonstrated that Bacillus velezensis KLP2016 (Gram +ve, endospore forming; Accession number KY214239) cell-free broth recorded an emulsification index (E 24 %) from 52.3% to 65.7% against different organic solvents, such as benzene, pentane, cyclohexane, xylene, n -hexane, toluene and engine oil. The surface tension of the cell-free broth of B. velezensis grown in Luria Bertani broth at 35°C decreased from 55 to 40 mN.m -1 at critical micelle concentration 17.2 µg/mL. The active biosurfactant molecule of cell-free broth of Bacillus velezensis KLP2016 was purified by Dietheylaminoethyl-cellulose and size exclusion chromatography, followed by HPLC (RT=1.130), UV-vis spectrophotometry (210 nm) and thin layer chromatography (R f =0.90). Purified biosurfactant molecular weight was found ~1.0 kDa, on the basis of Electron Spray Ionization-MS. A concentration of 1980×10 -2 parts per million of CO 2 was trapped in a KOH solution after 15 days incubating the bacterium in Luria Bertani broth containing engine oil (1%). Results suggests that bacterium Bacillus velezensis KLP2016 may be a promising solution to the engine oil pollution problem with achieving a bioactive biosurfactant molecule for further eco-friendly application(s).

scholarly journals A Novel Rhizospheric Bacterium: Bacillus velezensis NKMV-3 as a Biocontrol Agent Against Alternaria Leaf Blight in Tomato

2021 ◽  
Author(s):  
Murthy Vignesh ◽  
Shankar Ramakrishanan Madhan Shankar ◽  
Davoodbasha MubarakAli ◽  
Bodethala Narayanan Vedha Hari
Keyword(s):  
Biocontrol Agent ◽  
Leaf Blight ◽  
Bacillus Velezensis ◽  
Alternaria Leaf Blight ◽  
Bacterium Bacillus

scholarly journals Characterization and complete genome analysis of the surfactin-producing, plant-protecting bacterium Bacillus velezensis 9D-6

2019 ◽  
Vol 19 (1) ◽  
Author(s):  
Elliot Nicholas Grady ◽  
Jacqueline MacDonald ◽  
Margaret T. Ho ◽  
Brian Weselowski ◽  
Tim McDowell ◽  
...  
Keyword(s):  
Genome Analysis ◽  
Complete Genome ◽  
Bacillus Velezensis ◽  
Bacterium Bacillus ◽  
Complete Genome Analysis

scholarly journals Draft Genome Sequence of an Endophytic Biocontrol Bacterium, Bacillus velezensis PG12, Isolated from Apple Fruit

2019 ◽  
Vol 8 (41) ◽  
Author(s):  
Qingchao Zeng ◽  
Jianbo Xie ◽  
Yan Li ◽  
Xinyi Chen ◽  
Qi Wang
Keyword(s):  
Genome Sequence ◽  
Biocontrol Agent ◽  
Draft Genome ◽  
Pathogenic Fungi ◽  
Endophytic Bacterium ◽  
Apple Fruit ◽  
Draft Genome Sequence ◽  
Bacillus Velezensis ◽  
Content Type ◽  
Bacterium Bacillus

Bacillus velezensis PG12 is a biocontrol endophytic bacterium that is capable of inhibition of a broad range of plant-pathogenic fungi. The strain has the potential to be developed into a biocontrol agent for use in agriculture. Here, we report the draft genome sequence of B. velezensis PG12, which contains 22 scaffolds (3,990,845 bp), 3,884 coding sequences (CDSs), and an average G+C content of 46.45%.

scholarly journals Draft Genome Sequence of the Plant Growth-Promoting Bacterium Bacillus subtilis Strain BZR 517, Isolated from Winter Wheat, Now Reclassified as Bacillus velezensis Strain BZR 517

2020 ◽  
Vol 9 (40) ◽  
Author(s):  
Vitaly V. Radchenko ◽  
Ilya Y. Vasilyev ◽  
Elena V. Ilnitskaya ◽  
Alexey V. Garkovenko ◽  
Anzhela M. Asaturova ◽  
...  
Keyword(s):  
Plant Growth ◽  
Draft Genome ◽  
Biologically Active ◽  
Subtilis Strain ◽  
Bacillus Velezensis ◽  
Content Type ◽  
Plant Growth Promoting ◽  
Growth Promoting ◽  
Biocontrol Potential ◽  
Bacterium Bacillus

ABSTRACT Bacillus velezensis strain BZR 517 is a prospective plant growth-promoting rhizobacterium with known biocontrol properties, which may be used to improve soil quality. The genome sequencing was conducted as part of new biological agent development in order to determine the biocontrol potential of the strain, including the production of biologically active compounds.

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