scholarly journals Detection of Borrelia burgdorferi s.l., Anaplasma phagocytophilum and Babesia spp. in Dermacentor reticulatus ticks found within the city of Białystok, Poland—first data

2021 ◽  
Vol 85 (1) ◽  
pp. 63-73
Author(s):  
Anna Grochowska ◽  
Justyna Dunaj ◽  
Sławomir Pancewicz ◽  
Piotr Czupryna ◽  
Piotr Majewski ◽  
...  
Keyword(s):  
Borrelia Burgdorferi ◽  
Anaplasma Phagocytophilum ◽  
Animal Health ◽  
Dermacentor Reticulatus ◽  
Babesia Microti ◽  
Rural Landscapes ◽  
Babesia Vogeli ◽  
Borrelia Burgdorferi S.L ◽  
Babesia Venatorum ◽  
The City

AbstractPathogens carried by ticks pose a threat to both human and animal health across the world. Typically associated with rural landscapes, ticks appear to adapt well to life in urban recreational areas. Although Dermacentor reticulatus is commonly found across Europe, data on the prevalence of pathogens in this tick species, in an urban environment, are very limited. PCR was used to examine 368 D. reticulatus individuals collected in the Zwierzyniecki Forest Nature Reserve in Białystok, Poland. In total, 10.3% of ticks were infected, with Babesia spp. (9.2%), Anaplasma phagocytophilum (0.8%) and Borrelia burgdorferi sensu lato (0.3%). Rickettsia spp., Bartonella spp., and Coxiella burnetii were not detected. Sequence analysis for Babesia-positive samples identified 79.4% of them as Babesia canis, 8.8% as Babesia microti, 5.9% as Babesia spp., 2.9% as Babesia venatorum, and 2.9% as Babesia vogeli. Results obtained in this study indicate that D. reticulatus ticks found within the urban premises of the study area are infected with at least three pathogens and therefore are an important factor in public health risk for tick-borne diseases.

Molecular detection and identification of piroplasms (Babesia spp. and Theileria spp.) and Anaplasma phagocytophilum in questing ticks from northwest Spain

2020 ◽  
Author(s):  
Susana Remesar ◽  
Pablo Diaz ◽  
Alberto Prieto ◽  
David García-Dios ◽  
Rosario Panadero ◽  
...  
Keyword(s):  
Animal Health ◽  
Likelihood Estimation ◽  
Dermacentor Reticulatus ◽  
Species Level ◽  
Health Concern ◽  
Babesia Microti ◽  
Emerging Pathogens ◽  
Wide Range ◽  
Questing Ticks ◽  
Babesia Venatorum

Abstract Background: Ticks can transmit a wide range of pathogens; some of them are regarded as emerging or re-emerging pathogens causing a significant impact on human and animal health. Methods: In order to determine prevalence and zoonotic potential of Anaplasma spp., Candidatus Neoehrlichia mikurensis and piroplasms in questing ticks from north-western Spain, 1,056 Ixodes ricinus, nineteen Dermacentor marginatus, seventeen Dermacentor reticulatus, twelve Ixodes frontalis and a single Ixodes acuminatus were molecularly analysed. Also, 23 pools of I. ricinus larvae were investigated for the presence of piroplasms. Anaplasma and piroplasm DNA was firstly detected using two commercial qPCR assays. Samples positive to Anaplasma spp. were confirmed and identified at species level by sequence analysis of the groESL, 16S rRNA and msp2 genes. Those samples negative to Anaplasma spp. groESL were pooled and processed to detect a fragment of the groESL gene of Ca. N. mikurensis. qPCR piroplasm positive samples were molecularly identified at the species level by partial sequencing of the 18S rRNA and ITS1 genes.Results: Four pathogen species were detected in individual I. ricinus, namely Babesia venatorum (1.5%), A. phagocytophilum (0.7%), Babesia microti (0.3%) and Theileria sp. OT3 (0.2%). Babesia venatorum was also identified in a single I. ricinus larvae pool (maximum likelihood estimation 0.6%) whereas no I. ricinus pools were positive to Ca. N. mikurensis. In addition, one I. frontalis (8.3%) tested positive to A. phagocytophilum. Conclusions: Our results revealed that a low percentage of I. ricinus from northwest Spain were infected with A. phagocytophilum and piroplasms. Since a potentially pathogenic variant of A. phagocytophilum and two zoonotic Babesia species were detected, these results may have public health concern. Since the vector of Theileria sp. OT3 remains unknown, its identification in I. ricinus is especially interesting; nevertheless, further investigations are needed to unravel the role of I. ricinus in the transmission of this Theileria species.

scholarly journals Molecular Detection of Borrelia burgdorferi Sensu Lato and Anaplasma phagocytophilum in Ticks Collected from Dogs in Urban Areas of North-Eastern Poland

Pathogens ◽  
2020 ◽  
Vol 9 (6) ◽  
pp. 455
Author(s):  
Mirosław M. Michalski ◽  
Katarzyna Kubiak ◽  
Magdalena Szczotko ◽  
Marta Chajęcka ◽  
Małgorzata Dmitryjuk
Keyword(s):  
Borrelia Burgdorferi ◽  
Anaplasma Phagocytophilum ◽  
Urban Areas ◽  
Molecular Detection ◽  
Dermacentor Reticulatus ◽  
Borrelia Burgdorferi Sensu Lato ◽  
16S Rna ◽  
North Eastern ◽  
The City ◽  
Rna Genes

From 2016 to 2018, ticks were collected from 272 dogs admitted to veterinary clinics in the city of Olsztyn (north-eastern Poland). Among 522 collected ticks, 423 were identified as Ixodes ricinus (413 females and 10 males) and 99 as Dermacentor reticulatus (62 females and 37 males). Non-engorged (86 individuals) and engorged (436 individuals) ticks were screened for the presence of Borrelia burgdorferi sensu lato and Anaplasma phagocytophilum DNA. Borrelia and A. phagocytophilum species detection was determined based on the sequence of the fla B and 16S RNA genes, respectively. DNA of B. burgdorferi s.l. was identified in 31.6% (165/522, 95% CI: 27.6–35.8%) of ticks (I. ricinus 151/423, 35.7%, 95% CI: 31.1–40.4%; D. reticulates 14/99, 14.1%, 95% CI: 7.9–22.6%). A. phagocytophilum was identified in 0.96% (5/522, 95% CI: 0.3–2.2%) of specimens. All positive samples were engorged I. ricinus females (5/402, 1.2%, 95% CI: 0.4–2.9%). In 85.4% (141/165, 95% CI: 79.1–90.4%) of Borrelia infected ticks, the DNA of one genospecies was revealed. The DNA of at least two different genospecies was detected in 14.5% of specimens (24/165, 95% CI: 9.5–20.8). The coexistence of B. burgdorferii s.l. and A. phagocytophilum was not detected.

scholarly journals Novel Protozoans in Austria Revealed through the Use of Dogs as Sentinels for Ticks and Tick-Borne Pathogens

2021 ◽  
Vol 9 (7) ◽  
pp. 1392
Author(s):  
Michiel Wijnveld ◽  
Anna-Margarita Schötta ◽  
Theresa Stelzer ◽  
Georg Duscher ◽  
Michael Leschnik ◽  
...  
Keyword(s):  
Anaplasma Phagocytophilum ◽  
Blot Hybridization ◽  
Dermacentor Reticulatus ◽  
Sensu Stricto ◽  
Babesia Microti ◽  
Rickettsia Helvetica ◽  
Haemaphysalis Concinna ◽  
Borrelia Lusitaniae ◽  
Rickettsia Monacensis ◽  
Borrelia Burgdorferi Sensu Stricto

We previously isolated and cultivated the novel Rickettsia raoultii strain Jongejan. This prompted us to ask whether this strain is unique or more widely present in Austria. To assess this issue, we retrospectively screened ticks collected from dogs in 2008. Of these collected ticks, we randomly selected 75 (47 females and 28 males) Dermacentor reticulatus, 44 (21 females, 7 males, and 16 nymphs) Haemaphysalis concinna, and 55 (52 females and 3 males) ticks of the Ixodes ricinus complex. Subsequently, these ticks were individually screened for the presence of tick-borne pathogens using the reverse line blot hybridization assay. In our current study, we detected DNA from the following microbes in D. reticulatus: Anaplasma phagocytophilum, Borrelia lusitaniae, Borrelia spielmanii, Borrelia valaisiana, and R. raoultii, all of which were R. raoultii strain Jongejan. In H. concinna, we found DNA of a Babesia sp., Rickettsia helvetica, and an organism closely related to Theileria capreoli. Lastly, I. ricinus was positive for Anaplasma phagocytophilum, Borrelia afzelii, Borrelia burgdorferi sensu stricto, Borrelia garinii/Borrelia bavariensis, B. lusitaniae, B. spielmanii, B. valaisiana, Candidatus Neoehrlichia mikurensis, Rickettsia helvetica, Rickettsia monacensis, and Theileria (Babesia) microti DNA. The detection of DNA of the Babesia sp. and an organism closely related to Theileria capreoli, both found in H. concinna ticks, is novel for Austria.

scholarly journals Detection of Anaplasma phagocytophilum, Babesia odocoilei, Babesia sp., Borrelia burgdorferi Sensu Lato, and Hepatozoon canis in Ixodes scapularis Ticks Collected in Eastern Canada

Pathogens ◽  
2021 ◽  
Vol 10 (10) ◽  
pp. 1265
Author(s):  
John D. Scott ◽  
Risa R. Pesapane
Keyword(s):  
Borrelia Burgdorferi ◽  
Anaplasma Phagocytophilum ◽  
Ixodes Scapularis ◽  
Babesia Microti ◽  
Borrelia Burgdorferi Sensu Lato ◽  
Hepatozoon Canis ◽  
Eastern Canada ◽  
Zoonotic Pathogens ◽  
First Time ◽  
Established Population

Tick-borne pathogens cause infectious diseases that inflict much societal and financial hardship worldwide. Blacklegged ticks, Ixodes scapularis, are primary vectors of several epizootic and zoonotic pathogens. The aim sets forth the pathogens and their prevalence. In Ontario and Quebec, 113 I. scapularis ticks were collected from songbirds, mammals, including humans, and by flagging. PCR and DNA sequencing detected five different microorganisms: Anaplasma phagocytophilum, 1 (0.9%); Babesia odocoilei, 17 (15.3%); Babesia microti-like sp., 1 (0.9%); Borrelia burgdorferi sensu lato (Bbsl), 29 (26.1%); and Hepatozoon canis, 1 (0.9%). Five coinfections of Bbsl and Babesia odocoilei occurred. Notably, H. canis was documented for the first time in Canada and, at the same time, demonstrates the first transstadial passage of H. canis in I. scapularis. Transstadial passage of Bbsl and B. odocoilei was also witnessed. A novel undescribed piroplasm (Babesia microti-like) was detected. An established population of I. scapularis ticks was detected at Ste-Anne-de-Bellevue, Quebec. Because songbirds widely disperse I. scapularis larvae and nymphs, exposure in an endemic area is not required to contract tick-borne zoonoses. Based on the diversity of zoonotic pathogens in I. scapularis ticks, clinicians need to be aware that people who are bitten by I. scapularis ticks may require select antimicrobial regimens.

The PCR and nested PCR detection of Borrelia burgdorferi sensu lato, Anaplasma phagocytophilum and Babesia microti in Dermacentor reticulatus F. collected in a new location in Poland (Trzciel, Western Poland)

2016 ◽  
Vol 61 (4) ◽  
Author(s):  
Patrycja Opalińska ◽  
Anna Wierzbicka ◽  
Marek Asman
Keyword(s):  
Tree Species ◽  
Nested Pcr ◽  
Anaplasma Phagocytophilum ◽  
Dermacentor Reticulatus ◽  
Babesia Microti ◽  
Borrelia Burgdorferi Sensu Lato ◽  
Forest Landscape ◽  
The West ◽  
West Part ◽  
New Location

AbstractThe study was performed in the Trzciel Forest Districts which is located in the west part of Poland. The Scots pine is the main tree species creating forest landscape there.

scholarly journals Occurrence of Babesia Species and Co-Infection with Hepatozoon canis in Symptomatic Dogs and in Their Ticks in Eastern Romania

Pathogens ◽  
2021 ◽  
Vol 10 (10) ◽  
pp. 1339
Author(s):  
Lavinia Ciuca ◽  
Gabriela Martinescu ◽  
Liviu Dan Miron ◽  
Constantin Roman ◽  
Dumitru Acatrinei ◽  
...  
Keyword(s):  
Vector Competence ◽  
Rhipicephalus Sanguineus ◽  
Dermacentor Reticulatus ◽  
Babesia Microti ◽  
Hepatozoon Canis ◽  
Babesia Species ◽  
Lack Of Information ◽  
Babesia Vogeli ◽  
Babesia Rossi ◽  
Group 3

Although the distribution of Babesia spp. and Hepatozoon canis is well known in Romania, there is still a marked lack of information in many places of the country. This study aimed to investigate the occurrence of these haemoparasites in symptomatic dogs and in their ticks in Iasi, eastern Romania. Ninety owned dogs were subjected to clinical examination at the Faculty of Veterinary Medicine of Iasi and all detectable ticks (58 ticks from 15 dogs) were collected. Additionally, 124 ticks collected from the coat of other dogs (no. = 23) were included. Three Babesia species were found in dogs: Babesia canis (94.4%), Babesia vogeli (3.3%), and Babesia rossi (2.2%). All the dogs resulted negative for H. canis. The ticks were identified as follows: Ixodes ricinus (64%), Dermacentor reticulatus (33%), and Rhipicephalus sanguineus group (3%). B. canis (Minimum Infection Rate; MIR = 81%), B. vogeli (MIR = 3%), and Babesia microti-like piroplasm (MIR = 1%) were found in ticks. Moreover, 15 ticks were positive for H. canis, 6 were co-infected with B. canis, and 1 with B. microti-like piroplasm. This is the first molecular identification of B. rossi in two symptomatic dogs from Romania, although further studies are needed to investigate the vector competence of other ticks from Europe.

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