An In Situ Multispectral Imaging System for Planar Optodes in Sediments: Examples of High-Resolution Seasonal Patterns of pH

2011 ◽  
Vol 17 (4-5) ◽  
pp. 457-471 ◽  
Author(s):  
Yanzhen Fan ◽  
Qingzhi Zhu ◽  
Robert C. Aller ◽  
Donald C. Rhoads
Keyword(s):  
High Resolution ◽  
Multispectral Imaging ◽  
Imaging System ◽  
Seasonal Patterns

scholarly journals A Cost-Effective In Situ Zooplankton Monitoring System Based on Novel Illumination Optimization

Sensors ◽  
2020 ◽  
Vol 20 (12) ◽  
pp. 3471
Author(s):  
Zhiqiang Du ◽  
Chunlei Xia ◽  
Longwen Fu ◽  
Nan Zhang ◽  
Bowei Li ◽  
...  
Keyword(s):  
High Resolution ◽  
Imaging System ◽  
Physiological State ◽  
Cost Effective ◽  
In Situ Monitoring ◽  
Measuring System ◽  
Target Area ◽  
Image Processing Algorithm ◽  
Led Array

A cost-effective and low-power-consumption underwater microscopic imaging system was developed to capture high-resolution zooplankton images in real-time. In this work, dark-field imaging was adopted to reduce backscattering and background noise. To produce an accurate illumination, a novel illumination optimization scheme for the light-emitting diode (LED) array was proposed and applied to design a lighting system for the underwater optical imaging of zooplankton. A multiple objective genetic algorithm was utilized to find the best location of the LED array, which resulted in the specific illumination level and most homogeneous irradiance in the target area. The zooplankton imaging system developed with the optimal configuration of LEDs was tested with Daphnia magna under laboratory conditions. The maximal field of view was 16 mm × 13 mm and the optical resolution was 15 μm. The experimental results showed that the imaging system developed could capture high-resolution and high-definition images of Daphnia. Subsequently, Daphnia individuals were accurately segmented and their geometrical characters were measured by using a classical image processing algorithm. This work provides a cost-effective zooplankton measuring system based on an optimization illumination configuration of an LED array, which has a great potential for minimizing the investment and operating costs associated with long-term in situ monitoring of the physiological state and population conditions of zooplankton.

High-resolution multispectral imaging system for improved color acquisition and reproduction

2000 ◽  
Author(s):  
Juerg Haefliger ◽  
R. Stein ◽  
Heinrich Walt ◽  
Peter F. Niederer
Keyword(s):  
High Resolution ◽  
Multispectral Imaging ◽  
Imaging System

scholarly journals SoilCam: A Fully Automated Minirhizotron using Multispectral Imaging for Root Activity Monitoring

Sensors ◽  
2020 ◽  
Vol 20 (3) ◽  
pp. 787
Author(s):  
Gazi Rahman ◽  
Hanif Sohag ◽  
Rakibul Chowdhury ◽  
Khan A. Wahid ◽  
Anh Dinh ◽  
...  
Keyword(s):  
White Light ◽  
Multispectral Imaging ◽  
Imaging System ◽  
Image Sensor ◽  
Motion Blur ◽  
Image Resolution ◽  
In Situ Monitoring ◽  
Geometric Distortion ◽  
Entire Area

A minirhizotron is an in situ root imaging system that captures components of root system architecture dynamics over time. Commercial minirhizotrons are expensive, limited to white-light imaging, and often need human intervention. The implementation of a minirhizotron needs to be low cost, automated, and customizable to be effective and widely adopted. We present a newly designed root imaging system called SoilCam that addresses the above mentioned limitations. The imaging system is multi-modal, i.e., it supports both conventional white-light and multispectral imaging, with fully automated operations for long-term in-situ monitoring using wireless control and access. The system is capable of taking 360° images covering the entire area surrounding the tube. The image sensor can be customized depending on the spectral imaging requirements. The maximum achievable image quality of the system is 8 MP (Mega Pixel)/picture, which is equivalent to a 2500 DPI (dots per inch) image resolution. The length of time in the field can be extended with a rechargeable battery and solar panel connectivity. Offline image-processing software, with several image enhancement algorithms to eliminate motion blur and geometric distortion and to reconstruct the 360° panoramic view, is also presented. The system is tested in the field by imaging canola roots to show the performance advantages over commercial systems.

mRNA localization by Electron microscopic in situ hybridization

1991 ◽  
Vol 49 ◽  
pp. 430-431
Author(s):  
J. A. Pollock ◽  
M. Martone ◽  
T. Deerinck ◽  
M. H. Ellisman
Keyword(s):  
Electron Microscopy ◽  
High Resolution ◽  
Nucleic Acids ◽  
Recombinant Dna ◽  
Light And Electron Microscopy ◽  
Electron Microscopic ◽  
High Voltage Electron Microscopy ◽  
Functional Sites ◽  
Voltage Electron

Localization of specific proteins in cells by both light and electron microscopy has been facilitate by the availability of antibodies that recognize unique features of these proteins. High resolution localization studies conducted over the last 25 years have allowed biologists to study the synthesis, translocation and ultimate functional sites for many important classes of proteins. Recently, recombinant DNA techniques in molecular biology have allowed the production of specific probes for localization of nucleic acids by “in situ” hybridization. The availability of these probes potentially opens a new set of questions to experimental investigation regarding the subcellular distribution of specific DNA's and RNA's. Nucleic acids have a much lower “copy number” per cell than a typical protein, ranging from one copy to perhaps several thousand. Therefore, sensitive, high resolution techniques are required. There are several reasons why Intermediate Voltage Electron Microscopy (IVEM) and High Voltage Electron Microscopy (HVEM) are most useful for localization of nucleic acids in situ.

Ultrastructural analysis of the spatial distribution of MRNA

1992 ◽  
Vol 50 (1) ◽  
pp. 552-553
Author(s):  
Gary Bassell ◽  
Robert H. Singer
Keyword(s):  
Electron Microscopy ◽  
Spatial Distribution ◽  
In Situ Hybridization ◽  
High Resolution ◽  
Nucleic Acids ◽  
Colloidal Gold ◽  
Dna Probe ◽  
Nucleotide Analogs ◽  
Gold Particles

We have been investigating the spatial distribution of nucleic acids intracellularly using in situ hybridization. The use of non-isotopic nucleotide analogs incorporated into the DNA probe allows the detection of the probe at its site of hybridization within the cell. This approach therefore is compatible with the high resolution available by electron microscopy. Biotinated or digoxigenated probe can be detected by antibodies conjugated to colloidal gold. Because mRNA serves as a template for the probe fragments, the colloidal gold particles are detected as arrays which allow it to be unequivocally distinguished from background.

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