Study of Cytokine Profile of Cultured “Early” and “Late” Endothelial Progenitor Cells in Peripheral Blood of Chronic Heart Failure Patients after Mobilization Course with Granulocyte Colony-Stimulating Factor

2015 ◽  
Vol 160 (2) ◽  
pp. 249-251
Author(s):  
N. A. Bondarenko ◽  
O. V. Poveshchenko ◽  
A. P. Lykov ◽  
I. I. Kim ◽  
M. A. Surovtseva ◽  
...  
Keyword(s):  
Heart Failure ◽  
Chronic Heart Failure ◽  
Progenitor Cells ◽  
Endothelial Progenitor Cells ◽  
Peripheral Blood ◽  
Granulocyte Colony Stimulating Factor ◽  
Colony Stimulating Factor ◽  
Endothelial Progenitor ◽  
Heart Failure Patients ◽  
Stimulating Factor

scholarly journals Efficacy of G-CSF mobilization CD34+ progenitor cells depending on ischemic history and age of patients with chronic heart failure

2018 ◽  
Vol 16 (1) ◽  
pp. 75
Author(s):  
I. I. Kim ◽  
O. V. Poveshchenko ◽  
V. I. Konenkov ◽  
Ye. A. Pokushalov ◽  
A. B. Romanov ◽  
...  
Keyword(s):  
Heart Failure ◽  
Chronic Heart Failure ◽  
Progenitor Cells ◽  
Peripheral Blood ◽  
Granulocyte Colony Stimulating Factor ◽  
Colony Stimulating Factor ◽  
Heart Attacks ◽  
Time Period ◽  
Cd34 Cells ◽  
Stimulating Factor

It is shown that administration of G-CSF (Granulocyte Colony Stimulating Factor) to patients with chronic heart failure has a gematologic effect, while increasing the number of leukocytes and neutrophils in the peripheral blood. G-CSF leads to mobilization of progenitor cells (PCs) from the bone marrow into the peripheral blood. The basal content of CD34+ cells, the number of heart attacks and the time period from the last myocardial infarction effect the number of CD34+ cells after mobilization.

scholarly journals Effect of Periodic Granulocyte Colony-Stimulating Factor Administration on Endothelial Progenitor Cells and Different Monocyte Subsets in Pediatric Patients with Muscular Dystrophies

2016 ◽  
Vol 2016 ◽  
pp. 1-9 ◽  
Author(s):  
Andrzej Eljaszewicz ◽  
Dorota Sienkiewicz ◽  
Kamil Grubczak ◽  
Bożena Okurowska-Zawada ◽  
Grażyna Paszko-Patej ◽  
...  
Keyword(s):  
Progenitor Cells ◽  
Endothelial Progenitor Cells ◽  
Neuromuscular Disorders ◽  
Muscular Dystrophies ◽  
Clinical Effects ◽  
Granulocyte Colony Stimulating Factor ◽  
Colony Stimulating Factor ◽  
Monocyte Subsets ◽  
Endothelial Progenitor ◽  
Stimulating Factor

Muscular dystrophies (MD) are heterogeneous group of diseases characterized by progressive muscle dysfunction. There is a large body of evidence indicating that angiogenesis is impaired in muscles of MD patients. Therefore, induction of dystrophic muscle revascularization should become a novel approach aimed at diminishing the extent of myocyte damage. Recently, we and others demonstrated that administration of granulocyte colony-stimulating factor (G-CSF) resulted in clinical improvement of patients with neuromuscular disorders. To date, however, the exact mechanisms underlying these beneficial effects of G-CSF have not been fully understood. Here we used flow cytometry to quantitate numbers of CD34+ cells, endothelial progenitor cells, and different monocyte subsets in peripheral blood of pediatric MD patients treated with repetitive courses of G-CSF administration. We showed that repetitive cycles of G-CSF administration induced efficient mobilization of above-mentioned cells including cells with proangiogenic potential. These findings contribute to better understanding the beneficial clinical effects of G-CSF in pediatric MD patients.

scholarly journals Functional characteristics of peripheral blood mononuclear cells after administration of granulocyte colony-stimulating factor in patients with chronic heart failure

2015 ◽  
Vol 18 (1) ◽  
pp. 26
Author(s):  
O. V. Poveshchenko ◽  
I. I. Kim ◽  
N. A. Bondarenko ◽  
A. P. Lykov ◽  
A. F. Poveshchenko ◽  
...  
Keyword(s):  
Heart Failure ◽  
Chronic Heart Failure ◽  
Peripheral Blood Mononuclear Cells ◽  
Peripheral Blood ◽  
Mononuclear Cells ◽  
Granulocyte Colony Stimulating Factor ◽  
Colony Stimulating Factor ◽  
Peripheral Blood Mononuclear ◽  
Blood Mononuclear Cells ◽  
Stimulating Factor

It is shown that the introduction of granulocyte colony stimulating factor (G-CSF) in patients with chronic heart failure increases the functional activity of peripheral blood mononuclear cells (MNC). Enriching peripheral blood MNCs with progenitor cells (PC) by mobilization of bone marrow leads to an increase of their proliferative capacity both under spontaneous culture conditions and when stimulated with ConA mitogen and cytokines G-CSF. Proliferative activity of MNCs is found to increase, as the number of CD34+ PC starts growing. It is also noted that MNCs produce a wide range of cytokines with regulatory action after mobilization in a 48-hour culture in vitro. The findings suggest the feasibility of using MNCs after G-CSF mobilization for stimulation of reparative processes in the myocardium.

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